{"id":11721,"date":"2024-12-09T11:26:06","date_gmt":"2024-12-09T03:56:06","guid":{"rendered":"https:\/\/abtvn.com\/?post_type=product&p=11721"},"modified":"2025-10-09T09:33:37","modified_gmt":"2025-10-09T02:03:37","slug":"topspec-ihhnv-pcr-kit-sps-001","status":"publish","type":"product","link":"https:\/\/abtvn.com\/en\/product\/topspec-ihhnv-pcr-kit-sps-001\/","title":{"rendered":"TopSPEC\u00ae IHHNV PCR Kit (SPS-001)"},"content":{"rendered":"

Gi\u1edbi thi\u1ec7u TopSPEC \u00ae IHHNV PCR Kit <\/strong><\/h4>\n

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Infectious Hypodermal and Hematopoietic Necrosis (IHHN) in shrimp is caused by the Infectious Hypodermal & Hematopoietic Necrosis Virus. Once infected, the virus destroys gland cells, lymphoid cells, and nerve cells, and it can spread both vertically and horizontally. Shrimp often die in large numbers 10-20 days after stocking. Adverse environmental factors combined with the presence of the virus exacerbate the damage significantly.<\/p>\n

TopSPEC\u00ae IHHNV PCR Kit<\/strong><\/em> is designed to detect the presence of IHHNV in shrimp samples using PCR technology. The kit offers quick testing time, high accuracy, a simple procedure, and the ability to detect multiple pathogens in a single amplification reaction.<\/p>\n

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TopSPEC\u00ae IHHNV PCR Kit<\/span><\/strong><\/em><\/p>\n

Specification<\/strong><\/strong><\/h4>\n\n\n\n\n\n\n\n\n\n\n
Targets<\/strong><\/td>\nVirus <\/span>Infectious Hypodermal & Hematopoietic Necrosis<\/span><\/em> (IHHNV)<\/span><\/td>\n<\/tr>\n
Input sample<\/strong><\/td>\nGills, swimming legs, stool samples from shrimp<\/span><\/td>\n<\/tr>\n
Input sample volume<\/strong><\/td>\n200 \u00b5L<\/td>\n<\/tr>\n
DNA volume<\/strong><\/td>\n5 \u00b5L<\/td>\n<\/tr>\n
PCR time<\/strong><\/td>\n2 hours \u2013 2 hours 30 minutes (depending on the model)<\/td>\n<\/tr>\n
Specificity<\/strong><\/td>\nOnly IHHNV is detected<\/td>\n<\/tr>\n
Storage<\/strong><\/td>\n12 months at -20o<\/sup>C<\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\nIHHNV PCR Mix, negative control, positive control, PCR tube<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

The recommended extraction kit to use is: <\/strong>TopPURE \u00ae Highpure Viral extraction kit (EXS-021)<\/a><\/p>\n

The recommended electrophoresis kit to use is: <\/strong>TopSPEC\u00ae Agarose Electrophoresis (AEP-01)<\/a><\/p>\n

Result analysis<\/strong><\/p>\n

\u2013 After electrophoresis is completed, the results are visualized on a UV transilluminator or a gel documentation system equipped with UV light.<\/p>\n

\u2013 100bp DNA ladder used includes 10 lines from 100 to 1000 bp<\/p>\n

\u2013 Expected results are as follows:<\/p>\n