{"id":9169,"date":"2023-10-30T19:44:36","date_gmt":"2023-10-30T12:14:36","guid":{"rendered":"https:\/\/abtvn.com\/?p=9169"},"modified":"2023-12-04T10:54:24","modified_gmt":"2023-12-04T03:24:24","slug":"cac-chuoi-oligonucleotide-duoc-tong-hop-nhu-the-nao","status":"publish","type":"post","link":"https:\/\/abtvn.com\/en\/cac-chuoi-oligonucleotide-duoc-tong-hop-nhu-the-nao\/","title":{"rendered":"<strong>C\u00e1c chu\u1ed7i Oligonucleotide \u0111\u01b0\u1ee3c t\u1ed5ng h\u1ee3p nh\u01b0 th\u1ebf n\u00e0o?<\/strong>"},"content":{"rendered":"<h2 class=\"wp-block-heading\"><strong>1. Oligonucleotide l\u00e0 g\u00ec?<\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Oligonucleotides (hay Oligos) l\u00e0 nh\u1eefng ph\u00e2n t\u1eed DNA ho\u1eb7c RNA ng\u1eafn, \u0111\u01b0\u1ee3c t\u1ea1o th\u00e0nh t\u1eeb s\u1ef1 k\u1ebft n\u1ed1i c\u1ee7a nhi\u1ec1u ti\u1ec3u ph\u00e2n t\u1eed nucleotide, li\u00ean k\u1ebft v\u1edbi nhau \u0111\u1ec3 t\u1ea1o th\u00e0nh c\u00e1c polyme sinh h\u1ecdc chu\u1ed7i \u0111\u01a1n. C\u00e1c \u0111\u01a1n ph\u00e2n trong qu\u00e1 tr\u00ecnh t\u1ed5ng h\u1ee3p Oligo l\u00e0 c\u00e1c nucleoside phosphoramidite. Phosphoramidite c\u00f3 b\u1ea3n ch\u1ea5t l\u00e0 c\u00e1c nucleoside nh\u01b0ng \u0111\u01b0\u1ee3c bi\u1ebfn \u0111\u1ed5i b\u1edfi v\u00ec m\u1ed9t nucleoside trong t\u1ef1 nhi\u00ean mang r\u1ea5t nhi\u1ec1u nh\u00f3m hydroxyl (OH) v\u00e0 nh\u00f3m amino (NH2), c\u1ea3 hai nh\u00f3m n\u00e0y \u0111\u1ec1u c\u00f3 th\u1ec3 d\u1ec5 d\u00e0ng t\u01b0\u01a1ng t\u00e1c v\u1edbi nhi\u1ec1u h\u1ee3p ch\u1ea5t kh\u00e1c \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng trong qu\u00e1 tr\u00ecnh t\u1ed5ng h\u1ee3p DNA.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">M\u1ed9t ph\u00e2n t\u1eed phosphoramidite ti\u00eau chu\u1ea9n ch\u1ee9a phosphit thay v\u00ec nh\u00f3m phosphat trong nucleotide, k\u00e8m theo \u0111\u00f3 l\u00e0 4 nh\u00f3m b\u1ea3o v\u1ec7 bao g\u1ed3m: nh\u00f3m b\u1ea3o v\u1ec7 Dimethoxytrityl (DMT), Diisopropylamino, 2-cyanoethyl v\u00e0 nh\u00f3m bi\u1ec3n \u0111\u1ed5i c\u00f3 ch\u1ee9c n\u0103ng b\u1ea3o v\u1ec7 c\u00e1c nh\u00f3m amino c\u1ee7a baz\u01a1.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">C\u00f4ng d\u1ee5ng ph\u1ed5 bi\u1ebfn nh\u1ea5t c\u1ee7a Oligos d\u00f9ng nh\u01b0 c\u00e1c \u0111\u1ea7u d\u00f2 ph\u00e2n t\u1eed ho\u1eb7c \u0111o\u1ea1n m\u1ed3i (primer) cho ph\u1ea3n \u1ee9ng chu\u1ed7i polymerase (PCR) nh\u1eb1m khu\u1ebfch \u0111\u1ea1i s\u1ed1 l\u01b0\u1ee3ng DNA t\u1eeb m\u1eabu g\u1ed1c. C\u00e1c \u1ee9ng d\u1ee5ng c\u1ee7a \u0111o\u1ea1n m\u1ed3i oligo bao g\u1ed3m gi\u1ea3i tr\u00ecnh t\u1ef1 DNA, bi\u1ec3u hi\u1ec7n gen, nh\u00e2n b\u1ea3n v\u00e0 ch\u1ea9n \u0111o\u00e1n ph\u00e2n t\u1eed. \u1ede vai tr\u00f2 \u0111\u1ea7u d\u00f2, Oligos \u0111\u01b0\u1ee3c d\u00f9ng trong c\u00e1c quy tr\u00ecnh blotting nh\u01b0 southern blotting, northern blotting nh\u1eb1m s\u00e0ng l\u1ecdc c\u00e1c b\u1ec7nh di truy\u1ec1n ho\u1eb7c x\u00e1c \u0111\u1ecbnh c\u00e1c m\u1ea7m b\u1ec7nh c\u1ee5 th\u1ec3 trong ch\u1ea9n \u0111o\u00e1n sinh h\u1ecdc ph\u00e2n t\u1eed. Ngo\u00e0i ra, oligo \u0111\u01b0\u1ee3c \u0111ang \u0111\u01b0\u1ee3c nghi\u00ean c\u1ee9u ph\u00e2n t\u1eed trong \u0111i\u1ec1u tr\u1ecb, \u1ee9c ch\u1ebf gen hay \u1ee9ng d\u1ee5ng nucleotide t\u1ed5ng h\u1ee3p trong t\u01b0\u01a1ng lai.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">T\u1ed5ng h\u1ee3p oligonucleotide \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n tr\u00ean gi\u00e1 \u0111\u1ee1 r\u1eafn (c\u00f2n \u0111\u01b0\u1ee3c g\u1ecdi l\u00e0 resin) th\u01b0\u1eddng c\u00f3 \u0111\u01b0\u1eddng k\u00ednh 50-200 \u03bcm. Nhi\u1ec1u lo\u1ea1i gi\u00e1 \u0111\u1ee1 r\u1eafn \u0111\u00e3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng, nh\u01b0ng CPG (controlled pore glass) v\u00e0 polystyrene \u0111\u00e3 \u0111\u01b0\u1ee3c ch\u1ee9ng minh l\u00e0 h\u1eefu \u00edch nh\u1ea5t.<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-large is-resized\"><img fetchpriority=\"high\" decoding=\"async\" width=\"1024\" height=\"726\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2023\/10\/ABT-Oligonucleotide-synthesis-cycle-2-1024x726.png\" alt=\"Quy tr\u00ecnh t\u1ed5ng h\u1ee3p Oligonucleotide\" class=\"wp-image-9176\" style=\"aspect-ratio:1.3969986357435198;width:906px;height:auto\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2023\/10\/ABT-Oligonucleotide-synthesis-cycle-2-1024x726.png 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/10\/ABT-Oligonucleotide-synthesis-cycle-2-300x213.png 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/10\/ABT-Oligonucleotide-synthesis-cycle-2-768x544.png 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/10\/ABT-Oligonucleotide-synthesis-cycle-2-1536x1089.png 1536w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/10\/ABT-Oligonucleotide-synthesis-cycle-2-18x12.png 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/10\/ABT-Oligonucleotide-synthesis-cycle-2-1707x1210.png 1707w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/10\/ABT-Oligonucleotide-synthesis-cycle-2.png 1799w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"wp-element-caption\"><strong><em>H\u00ecnh 1. Quy tr\u00ecnh t\u1ed5ng h\u1ee3p Oligonucleotides<\/em><\/strong><\/figcaption><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>2. Quy tr\u00ecnh t\u1ed5ng h\u1ee3p Oligonucleotide<\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Qu\u00e1 tr\u00ecnh t\u1ed5ng h\u1ee3p Oligonucleotide s\u1eed d\u1ee5ng phosphoramidite c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c ti\u1ebfn h\u00e0nh trong dung d\u1ecbch ho\u1eb7c tr\u00ean pha r\u1eafn. C\u00e1c tr\u00ecnh t\u1ef1 oligonucleotide c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c l\u1eafp r\u1eafp t\u1eebng b\u01b0\u1edbc theo chi\u1ec1u t\u1eeb 3\u2019 \u0111\u1ebfn 5\u2019 (ng\u01b0\u1ee3c l\u1ea1i v\u1edbi chi\u1ec1u t\u1ed5ng h\u1ee3p DNA trong qu\u00e1 tr\u00ecnh sao ch\u00e9p DNA) b\u1eb1ng c\u00e1ch l\u1eb7p l\u1ea1i c\u00e1c chu k\u1ef3 t\u1ed5ng h\u1ee3p. M\u1ed7i chu tr\u00ecnh t\u1ed5ng h\u1ee3p bao g\u1ed3m b\u1ed1n b\u01b0\u1edbc l\u00e0 b\u1ed1n ph\u1ea3n \u1ee9ng h\u00f3a h\u1ecdc bao g\u1ed3m: kh\u1eed DMT (Deblocking), gh\u00e9p n\u1ed1i (Coupling), kh\u00f3a (Capping) v\u00e0 oxy h\u00f3a (Oxidation).<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>Step 1:<\/strong> <strong>Kh\u1eed DMT<\/strong> (<strong>Detritylation<\/strong>)<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">Nh\u00f3m b\u1ea3o v\u1ec7 DMT (4,4&#8242;-dimethoxytrityl) \u1edf \u0111\u1ea7u 5\u2019 c\u1ee7a chu\u1ed7i DNA li\u00ean k\u1ebft v\u1edbi resin ph\u1ea3i \u0111\u01b0\u1ee3c lo\u1ea1i b\u1ecf \u0111\u1ec3 gi\u1ea3i ph\u00f3ng nh\u00f3m 5\u2019-OH nh\u1eb1m gh\u00e9p n\u1ed1i v\u1edbi phosphoramidite ti\u1ebfp theo. Qu\u00e1 tr\u00ecnh lo\u1ea1i b\u1ecf nh\u00f3m b\u1ea3o v\u1ec7 DMT n\u00e0y c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c lo\u1ea1i b\u1ecf d\u01b0\u1edbi \u0111i\u1ec1u ki\u1ec7n axit, ch\u1eb3ng h\u1ea1n nh\u01b0 dung d\u1ecbch axit Trichloroacetic Acid (TCA) 3% ho\u1eb7c Dichloroacetic Acid (DCA) 3%.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>B\u01b0\u1edbc 2: Gh\u00e9p n\u1ed1i (Coupling)<\/strong><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">Sau qu\u00e1 tr\u00ecnh kh\u1eed trityl, phosphoramidite \u0111\u00e3 kh\u1eed DMT s\u1eb5n s\u00e0ng ph\u1ea3n \u1ee9ng v\u1edbi base ti\u1ebfp theo, \u0111\u01b0\u1ee3c th\u00eam v\u00e0o d\u01b0\u1edbi d\u1ea1ng \u0111\u01a1n ph\u00e2n monome nucleoside phosphoramidite. M\u1ed9t l\u01b0\u1ee3ng nucleoside phosphoramidite \u0111\u01b0\u1ee3c tr\u1ed9n v\u1edbi c\u00e1c dung d\u1ecbch gh\u00e9p n\u1ed1i (ch\u1ea5t ho\u1ea1t h\u00f3a &#8211; Activator solution) nh\u01b0 1H-tetrazole, \u0111\u01b0\u1ee3c h\u00f2a tan trong dung m\u00f4i acetonitrile. Nh\u00f3m amino c\u1ee7a nucleoside phosphoramidite \u0111\u01b0\u1ee3c proton h\u00f3a v\u00e0 sau \u0111\u00f3 nhanh ch\u00f3ng \u0111\u01b0\u1ee3c thay th\u1ebf b\u1eb1ng nh\u00f3m 5&#8242;-hydroxyl (5\u2019OH) c\u1ee7a phosphoramidite v\u00e0 m\u1ed9t li\u00ean k\u1ebft c\u1ee7a triester phosphite v\u1edbi gi\u00e1 th\u1ec3 \u0111\u01b0\u1ee3c h\u00ecnh th\u00e0nh.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>B\u01b0\u1edbc 3: Kh\u00f3a (Capping)<\/strong><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">Kh\u00f4ng ph\u1ea3i l\u00fac n\u00e0o hi\u1ec7u su\u1ea5t trong m\u1ed7i b\u01b0\u1edbc gh\u00e9p n\u1ed1i c\u0169ng \u0111\u1ea1t \u0111\u01b0\u1ee3c ng\u01b0\u1ee1ng l\u00fd t\u01b0\u1edfng 100%, v\u1eabn c\u00f2n m\u1ed9t v\u00e0i nh\u00f3m 5&#8242;-hydroxyl ch\u01b0a ph\u1ea3n \u1ee9ng tr\u00ean nucleotide li\u00ean k\u1ebft v\u1edbi resin.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">V\u00ec th\u1ebf, n\u1ebfu kh\u00f4ng \u0111\u01b0\u1ee3c ki\u1ec3m tra lo\u1ea1i b\u1ecf, c\u00e1c nh\u00f3m 5&#8242;-hydroxyl n\u00e0y s\u1ebd s\u1eb5n s\u00e0ng \u0111\u1ec3 tham gia v\u00e0o b\u01b0\u1edbc gh\u00e9p n\u1ed1i ti\u1ebfp theo. \u0110\u1ec3 ng\u0103n ch\u1eb7n \u0111i\u1ec1u n\u00e0y, b\u01b0\u1edbc \u201ccapping\u201d \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n sau ph\u1ea3n \u1ee9ng gh\u00e9p n\u1ed1i gi\u00fap kh\u00f3a v\u00e0 ng\u0103n ch\u1eb7n c\u00e1c nh\u00f3m 5&#8242;-hydroxyl ch\u01b0a ph\u1ea3n \u1ee9ng b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng hai dung d\u1ecbch Capping: acetic anhydride v\u00e0 N-methylimidazole (NMI). Hai dung d\u1ecbch n\u00e0y \u0111\u01b0\u1ee3c h\u00f2a tan trong tetrahydrofuran ho\u1eb7c acetonitril (Capping A) \u0111\u01b0\u1ee3c tr\u1ed9n v\u1edbi dung d\u1ecbch N-methylimidazole (Capping B) c\u00f3 t\u00e1c d\u1ee5ng x\u00fac t\u00e1c. C\u00e1c nh\u00f3m hydroxyl t\u1ef1 do \u0111\u01b0\u1ee3c chuy\u1ec3n \u0111\u1ed5i th\u00e0nh axetat v\u00e0 b\u1ecb kh\u00f3a l\u1ea1i, ng\u0103n ch\u1eb7n s\u1ef1 kh\u1eed trityl v\u00e0 ng\u0103n s\u1ef1 k\u00e9o d\u00e0i chu\u1ed7i ti\u1ebfp theo c\u0169ng nh\u01b0 h\u00ecnh th\u00e0nh c\u00e1c oligonucleotide d\u00e0i v\u1edbi tr\u00ecnh t\u1ef1 kh\u00f4ng ch\u00ednh x\u00e1c.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>B\u01b0\u1edbc 4: Oxi h\u00f3a (Oxidation)<\/strong><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">Li\u00ean k\u1ebft Phosphite-triester (P(III)) \u0111\u01b0\u1ee3c h\u00ecnh th\u00e0nh trong b\u01b0\u1edbc gh\u00e9p n\u1ed1i kh\u00f4ng b\u1ec1n v\u1edbi axit v\u00e0 ph\u1ea3i \u0111\u01b0\u1ee3c chuy\u1ec3n \u0111\u1ed5i th\u00e0nh d\u1ea1ng h\u00f3a tr\u1ecb n\u0103m (V) \u1ed5n \u0111\u1ecbnh tr\u01b0\u1edbc khi b\u01b0\u1edbc qua qu\u00e1 tr\u00ecnh kh\u1eed DMT ti\u1ebfp theo. \u0110i\u1ec1u n\u00e0y \u0111\u1ea1t \u0111\u01b0\u1ee3c b\u1eb1ng c\u00e1ch oxy h\u00f3a i\u1ed1t v\u1edbi s\u1ef1 c\u00f3 m\u1eb7t c\u1ee7a n\u01b0\u1edbc v\u00e0 pyridin. \u1ede b\u01b0\u1edbc n\u00e0y, nh\u00f3m b\u1ea3o v\u1ec7 cyanoethyl ng\u0103n ch\u1eb7n c\u00e1c ph\u1ea3n \u1ee9ng kh\u00f4ng mong mu\u1ed1n v\u1edbi phosphorus trong c\u00e1c chu k\u1ef3 t\u1ed5ng h\u1ee3p ti\u1ebfp theo. Chu k\u1ef3 \u0111\u01b0\u1ee3c l\u1eb7p l\u1ea1i, m\u1ed9t l\u1ea7n \u0111\u1ed1i v\u1edbi m\u1ed7i base, \u0111\u1ec3 t\u1ea1o ra oligonucleotide c\u1ea7n thi\u1ebft.&nbsp;<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Sau khi ho\u00e0n th\u00e0nh qu\u00e1 tr\u00ecnh t\u1ed5ng h\u1ee3p chu\u1ed7i, qu\u00e1 tr\u00ecnh c\u1eaft oligonucleotide kh\u1ecfi gi\u00e1 th\u1ec3 r\u1eafn v\u00e0 kh\u1eed b\u1ea3o v\u1ec7 s\u1ebd gi\u00fap k\u1ebft th\u00fac qu\u00e1 tr\u00ecnh t\u1ed5ng h\u1ee3p Oligonucleotide th\u00f4 (ch\u01b0a \u0111\u01b0\u1ee3c tinh s\u1ea1ch). Amonium hydroxide \u0111\u01b0\u1ee3c d\u00f9ng trong qu\u00e1 tr\u00ecnh c\u1eaft v\u00e0 kh\u1eed b\u1ea3o v\u1ec7 ti\u00eau chu\u1ea9n. H\u00f3a ch\u1ea5t AMA (h\u1ed7n h\u1ee3p 1:1 gi\u1eefa metylamin v\u00e0 amonium hydroxide) v\u00e0 phosphoramidite ultramild trong Capping A \u0111\u01b0\u1ee3c d\u00f9ng t\u01b0\u01a1ng \u1ee9ng v\u1edbi trong qu\u00e1 tr\u00ecnh c\u1eaft v\u00e0 kh\u1eed b\u1ea3o v\u1ec7 nhanh (fast) v\u00e0 nh\u1eb9 (mild).<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Sau khi ho\u00e0n th\u00e0nh b\u01b0\u1edbc c\u1eaft v\u00e0 kh\u1eed b\u1ea3o v\u1ec7, oligo \u0111\u01b0\u1ee3c tinh s\u1ea1ch b\u1eb1ng c\u00e1c ph\u01b0\u01a1ng ph\u00e1p kh\u00e1c nhau t\u00f9y thu\u1ed9c v\u00e0o chi\u1ec1u d\u00e0i, \u1ee9ng d\u1ee5ng, chi ph\u00ed, v.v. M\u1ed9t s\u1ed1 ph\u01b0\u01a1ng ph\u00e1p tinh s\u1ea1ch ph\u1ed5 bi\u1ebfn nh\u01b0: kh\u1eed mu\u1ed1i, cartridge (DMT-ON), RP-HPLC, v.v.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Trong \u0111\u00f3, <a href=\"https:\/\/oligox.abtvn.com\/\" data-type=\"link\" data-id=\"https:\/\/oligox.abtvn.com\/\">OligoX &#8211; D\u1ecbch v\u1ee5 t\u1ed5ng h\u1ee3p Oligonucleotides<\/a> c\u1ee7a ABT l\u1ef1a ch\u1ecdn 2 ph\u01b0\u01a1ng ph\u00e1p kh\u1eed mu\u1ed1i, cartridge (DMT-ON) nh\u1eb1m t\u1ed1i \u01b0u \u0111\u1ed9 tinh s\u1ea1ch c\u1ee7a s\u1ea3n ph\u1ea9m v\u00e0 chi ph\u00ed \u0111\u1ed1i v\u1edbi kh\u00e1ch h\u00e0ng.<\/strong><\/p>\n\n\n\n<div class=\"wp-block-getwid-advanced-spacer\" style=\"height:31px\" aria-hidden=\"true\"><\/div>\n\n\n\n<div class=\"wp-block-uagb-buttons uagb-buttons__outer-wrap uagb-btn__default-btn uagb-btn-tablet__default-btn uagb-btn-mobile__default-btn uagb-block-7b318d4f\"><div class=\"uagb-buttons__wrap uagb-buttons-layout-wrap\">\n<div class=\"wp-block-uagb-buttons-child uagb-buttons__outer-wrap uagb-block-920f4fe9 wp-block-button\"><div class=\"uagb-button__wrapper\"><a class=\"uagb-buttons-repeater wp-block-button__link\" href=\"https:\/\/oligox.abtvn.com\/\" onclick=\"return true;\" rel=\"follow noopener\" target=\"_blank\"><div class=\"uagb-button__link\">OligoX  i Xem th\u00eam t\u1ea1i \u0111\u00e2y<\/div><span class=\"uagb-button__icon uagb-button__icon-position-after\"><svg xmlns=\"https:\/\/www.w3.org\/2000\/svg\" viewbox=\"0 0 448 512\"><path d=\"M438.6 278.6l-160 160C272.4 444.9 264.2 448 256 448s-16.38-3.125-22.62-9.375c-12.5-12.5-12.5-32.75 0-45.25L338.8 288H32C14.33 288 .0016 273.7 .0016 256S14.33 224 32 224h306.8l-105.4-105.4c-12.5-12.5-12.5-32.75 0-45.25s32.75-12.5 45.25 0l160 160C451.1 245.9 451.1 266.1 438.6 278.6z\"><\/path><\/svg><\/span><\/a><\/div><\/div>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-getwid-advanced-spacer\" style=\"height:31px\" aria-hidden=\"true\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>3. \u0110i\u1ec1u ki\u1ec7n b\u1eaft bu\u1ed9c trong t\u1ed5ng h\u1ee3p Oligonucleotides<\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Trong qu\u00e1 tr\u00ecnh t\u1ed5ng h\u1ee3p Oligo, tr\u00ecnh t\u1ef1 nucleotide c\u00e0ng d\u00e0i v\u00e0 c\u00e0ng ph\u1ee9c t\u1ea1p th\u00ec c\u00e0ng kh\u00f3 \u0111\u00e1p \u1ee9ng c\u00e1c m\u1ee5c ti\u00eau v\u1ec1 ch\u1ea5t l\u01b0\u1ee3ng, \u0111\u1ed9 tinh s\u1ea1ch v\u00e0 chi ph\u00ed. Vi\u1ec7c \u0111\u1ea3m b\u1ea3o c\u00e1c y\u00eau c\u1ea7u v\u1ec1 ki\u1ec3m so\u00e1t nhi\u1ec7t \u0111\u1ed9, pH, \u0111\u1ed9 \u1ea9m, c\u00e1c ch\u1ea5t c\u00f3 kh\u1ea3 n\u0103ng g\u00e2y nhi\u1ec5m hay b\u1ee5i m\u1ecbn r\u1ea5t quan tr\u1ecdng, \u0111\u00f3ng vai tr\u00f2 trong vi\u1ec7c \u0111\u00e1p \u1ee9ng c\u00e1c y\u00eau c\u1ea7u tr\u00ean.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Nhi\u1ec7t \u0111\u1ed9, \u0111\u1ed9 \u1ea9m<\/strong>:<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u0110\u1ed9 \u1ea9m c\u00f3 th\u1ec3 t\u00e1c \u0111\u1ed9ng \u0111\u1ebfn t\u1ed1c \u0111\u1ed9 v\u00e0 hi\u1ec7u su\u1ea5t c\u1ee7a c\u00e1c ph\u1ea3n \u1ee9ng h\u00f3a h\u1ecdc trong qu\u00e1 tr\u00ecnh t\u1ed5ng h\u1ee3p oligonucleotide. Trong qu\u00e1 tr\u00ecnh t\u1ed5ng h\u1ee3p, c\u00f3 th\u1ec3 xu\u1ea5t hi\u1ec7n c\u00e1c s\u1ea3n ph\u1ea9m ph\u1ee5 ho\u1eb7c ch\u1ea5t c\u1eb7n kh\u00f4ng mong mu\u1ed1n. \u0110\u1ed9 \u1ea9m c\u00f3 th\u1ec3 \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn s\u1ef1 t\u1ea1o th\u00e0nh c\u1ee7a nh\u1eefng impurities n\u00e0y. S\u1ef1 thi\u1ebfu h\u1ee5t ho\u1eb7c qu\u00e1 nhi\u1ec1u \u0111\u1ed9 \u1ea9m \u0111\u1ec1u c\u00f3 th\u1ec3 g\u00e2y ph\u00e1 v\u1ee1 ho\u1eb7c l\u00e0m m\u1ea5t t\u00ednh ch\u1ea5t c\u1ee7a oligonucleotide \u0111\u00e3 t\u1ed5ng h\u1ee3p.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>\u0110\u1ed9 pH<\/strong>:<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u0110\u1ed9 pH \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn t\u1ed1c \u0111\u1ed9 c\u1ee7a c\u00e1c ph\u1ea3n \u1ee9ng h\u00f3a h\u1ecdc trong qu\u00e1 tr\u00ecnh t\u1ed5ng h\u1ee3p. Nhi\u1ec1u ph\u1ea3n \u1ee9ng t\u1ed5ng h\u1ee3p oligonucleotide c\u1ea7n m\u00f4i tr\u01b0\u1eddng, dung m\u00f4i c\u00f3 \u0111\u1ed9 pH c\u1ee5 th\u1ec3 \u0111\u1ec3 di\u1ec5n ra hi\u1ec7u qu\u1ea3. \u0110i\u1ec1u n\u00e0y bao g\u1ed3m c\u1ea3 c\u00e1c b\u01b0\u1edbc li\u00ean quan \u0111\u1ebfn ph\u1ea3n \u1ee9ng gh\u00e9p n\u1ed1i, c\u1eaft v\u00e0 kh\u1eed b\u1ea3o v\u1ec7 v\u00e0 c\u00e1c b\u01b0\u1edbc x\u1eed l\u00fd kh\u00e1c.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>B\u1ee5i m\u1ecbn v\u00e0 ch\u1ea5t g\u00e2y nhi\u1ec5m (contaminant)<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">B\u1ee5i m\u1ecbn v\u00e0 ch\u1ea5t c\u00f3 kh\u1ea3 n\u0103ng g\u00e2y nhi\u1ec5m c\u00f3 th\u1ec3 ch\u1ee9a c\u00e1c t\u00e1c nh\u00e2n nhi\u1ec5m \u0111\u1ed9c ho\u1eb7c enzyme ngo\u1ea1i lai c\u00f3 th\u1ec3 l\u00e0m \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn qu\u00e1 tr\u00ecnh t\u1ed5ng h\u1ee3p. N\u1ebfu ch\u00fang ti\u1ebfp x\u00fac v\u1edbi m\u1eabu oligonucleotide ho\u1eb7c c\u00e1c d\u1ee5ng c\u1ee5 trong ph\u00f2ng th\u00ed nghi\u1ec7m, ch\u00fang c\u00f3 th\u1ec3 g\u00e2y nhi\u1ec5m b\u1ea9n cho s\u1ea3n ph\u1ea9m cu\u1ed1i c\u00f9ng v\u00e0 t\u1ea1o ra c\u00e1c ph\u1ea3n \u1ee9ng kh\u00f4ng mong mu\u1ed1n.<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-large is-resized\"><img decoding=\"async\" width=\"1024\" height=\"683\" data-src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2023\/10\/tu-atsh-2-1024x683.png\" alt=\"T\u1ed5ng h\u1ee3p Oligonucleotide\" class=\"wp-image-9173 lazyload\" style=\"--smush-placeholder-width: 1024px; --smush-placeholder-aspect-ratio: 1024\/683;aspect-ratio:1.499267935578331;width:910px;height:auto\" data-srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2023\/10\/tu-atsh-2-1024x683.png 1024w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/10\/tu-atsh-2-300x200.png 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/10\/tu-atsh-2-768x512.png 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/10\/tu-atsh-2-1536x1024.png 1536w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/10\/tu-atsh-2-18x12.png 18w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/10\/tu-atsh-2-1707x1138.png 1707w, https:\/\/abtvn.com\/wp-content\/uploads\/2023\/10\/tu-atsh-2.png 1875w\" data-sizes=\"(max-width: 1024px) 100vw, 1024px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" \/><figcaption class=\"wp-element-caption\"><strong><em>H\u00ecnh 2: T\u1ee7 An to\u00e0n Sinh h\u1ecdc c\u1ea5p II do ABT s\u1ea3n xu\u1ea5t<\/em><\/strong><\/figcaption><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\">V\u00ec v\u1eady, c\u00e1c \u0111i\u1ec1u ki\u1ec7n v\u1ec1 \u0111\u1ed9 \u1ea9m, \u0111\u1ed9 pH, ch\u1ea5t c\u00f3 kh\u1ea3 n\u0103ng g\u00e2y nhi\u1ec5m v.v lu\u00f4n \u0111\u01b0\u1ee3c ki\u1ec3m so\u00e1t nghi\u00eam ng\u1eb7t v\u00e0 c\u1ea7n \u0111\u01b0\u1ee3c thao t\u00e1c trong <strong><a href=\"https:\/\/abtvn.com\/en\/huong-dan-su-dung-tu-an-toan-sinh-hoc-cap-ii-abt\/\" data-type=\"link\" data-id=\"https:\/\/abtvn.com\/huong-dan-su-dung-tu-an-toan-sinh-hoc-cap-ii-abt\/\">T\u1ee7 An to\u00e0n Sinh h\u1ecdc<\/a><\/strong> trong c\u00e1c b\u01b0\u1edbc h\u1ed7 tr\u1ee3 qu\u00e1 tr\u00ecnh t\u1ed5ng h\u1ee3p Oligonucleotides nh\u1eb1m \u0111\u1ea3m b\u1ea3o \u0111\u1ed9 ch\u00ednh x\u00e1c v\u00e0 an to\u00e0n cho ng\u01b0\u1eddi thao t\u00e1c th\u00ed nghi\u1ec7m.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Xem th\u00eam chi ti\u1ebft v\u1ec1 <strong><a href=\"https:\/\/abtvn.com\/en\/product\/tu-an-toan-sinh-hoc-cap-ii-bcs-w1000\/\" target=\"_blank\" rel=\"noreferrer noopener\">Biological Safety Cabinet Level II (BCS-W1000)<\/a><\/strong> t\u1ea1i \u0111\u00e2y.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>C\u00e1c th\u00f4ng s\u1ed1 t\u1ee7 An to\u00e0n Sinh h\u1ecdc c\u1ea5p II:<\/strong><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u0110i\u1ec7n \u00e1p: 220V\/50hz<\/li>\n\n\n\n<li>T\u1ed1c \u0111\u1ed9 kh\u00ed trung b\u00ecnh: \u22650.4m\/s<\/li>\n\n\n\n<li>T\u1ed1c \u0111\u1ed9 kh\u00ed xu\u1ed1ng: 0.25-0.5m\/s<\/li>\n\n\n\n<li>C\u01b0\u1eddng \u0111\u1ed9 UV: \u22650.4\u00b5w\/cm\u00b2<\/li>\n\n\n\n<li>C\u01b0\u1eddng \u0111\u1ed9 s\u00e1ng: &gt;430 Lux<\/li>\n\n\n\n<li>V\u1eadt li\u1ec7u: INOX 304<\/li>\n\n\n\n<li>\u0110i\u1ec1u khi\u1ec3n: M\u00e0n h\u00ecnh c\u00f3 n\u00fat nh\u1ea5n<\/li>\n\n\n\n<li>5mm thick tempered glass sliding door<\/li>\n\n\n\n<li>L\u1ecdc HEPA: 99,99% \u0111\u1ed1i v\u1edbi c\u00e1c h\u1ea1t c\u00f3 k\u00edch th\u01b0\u1edbt 0.3 micro<\/li>\n\n\n\n<li>K\u00edch th\u01b0\u1edbc model: BSC-W1000\n<ul class=\"wp-block-list\">\n<li>K\u00edch th\u01b0\u1edbc ngo\u00e0i LxWxH:1000x740x1200<\/li>\n\n\n\n<li>K\u00edch th\u01b0\u1edbc bu\u1ed3ng thao t\u00e1c:935x590x540<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li>K\u00edch th\u01b0\u1edbc model: BSC-W1200\n<ul class=\"wp-block-list\">\n<li>K\u00edch th\u01b0\u1edbc ngo\u00e0i LxWxH:1200x800x1314<\/li>\n\n\n\n<li>K\u00edch th\u01b0\u1edbc bu\u1ed3ng thao t\u00e1c:1133x646x538<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"><strong><strong>Highlights<\/strong><\/strong>:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>C\u00f3 h\u1ec7 th\u1ed1ng m\u00e0ng l\u1ecdc HEPA l\u1ecdc \u0111\u01b0\u1ee3c 99,99% c\u00e1c h\u1ea1t c\u00f3 k\u00edch th\u01b0\u1edbc 0,3 micro<\/li>\n\n\n\n<li>70% kh\u00ed c\u1ea5p kh\u00ed xu\u1ed1ng khu v\u1ef1c l\u00e0m vi\u1ec7c, 30% ra ngo\u00e0i m\u00f4i tr\u01b0\u1eddng<\/li>\n\n\n\n<li>C\u00f3 c\u1ea3m bi\u1ebfn h\u00e0nh tr\u00ecnh l\u00e0m vi\u1ec7c \u0111\u1ea3m b\u1ea3o an to\u00e0n cho ng\u01b0\u1eddi s\u1eed d\u1ee5ng v\u00e0 b\u1ed9 \u0111i\u1ec1u khi\u1ec3n b\u1eb1ng n\u00fat nh\u1ea5n v\u00e0 m\u00e0n h\u00ecnh<\/li>\n\n\n\n<li>Thi\u1ebft k\u1ebf module gi\u00fap l\u1eafp \u0111\u1eb7t d\u1ec5 d\u00e0ng, v\u1eadt li\u1ec7u inox cao c\u1ea5p, ch\u1ed1ng ch\u1ecbu h\u00f3a ch\u1ea5t v\u00e0 d\u1ec5 d\u00e0ng v\u1ec7 sinh, th\u1ea9m m\u1ef9 cao<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>T\u00e0i li\u1ec7u tham kh\u1ea3o<\/strong><\/h3>\n\n\n\n<ol class=\"wp-block-list\">\n<li>Hao M, Qiao J, Qi H. Current and Emerging Methods for the Synthesis of Single-Stranded DNA. Genes (Basel). 2020 Jan 21;11(2):116. doi: 10.3390\/genes11020116. PMID: 31973021; PMCID: PMC7073533. <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/31973021\/\" target=\"_blank\" rel=\"noopener\">https:\/\/pubmed.ncbi.nlm.nih.gov\/31973021\/<\/a><\/li>\n<\/ol>","protected":false},"excerpt":{"rendered":"<p>Oligonucleotides (hay Oligos) l\u00e0 nh\u1eefng ph\u00e2n t\u1eed DNA ho\u1eb7c RNA ng\u1eafn, \u0111\u01b0\u1ee3c t\u1ea1o th\u00e0nh t\u1eeb s\u1ef1 k\u1ebft n\u1ed1i c\u1ee7a nhi\u1ec1u ti\u1ec3u ph\u00e2n t\u1eed nucleotide, li\u00ean k\u1ebft v\u1edbi nhau \u0111\u1ec3 t\u1ea1o th\u00e0nh c\u00e1c polyme sinh h\u1ecdc chu\u1ed7i \u0111\u01a1n.<\/p>","protected":false},"author":1,"featured_media":9177,"comment_status":"open","ping_status":"open","sticky":true,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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