{"id":7866,"date":"2024-02-23T15:31:28","date_gmt":"2024-02-23T08:01:28","guid":{"rendered":"https:\/\/abtvn.com\/?p=7866"},"modified":"2025-10-22T16:34:08","modified_gmt":"2025-10-22T09:04:08","slug":"chi-tiet-ve-do-tai-luong-hbv-bang-real-time-pcr","status":"publish","type":"post","link":"https:\/\/abtvn.com\/en\/chi-tiet-ve-do-tai-luong-hbv-bang-real-time-pcr\/","title":{"rendered":"Chi ti\u1ebft v\u1ec1 \u0111o t\u1ea3i l\u01b0\u1ee3ng HBV b\u1eb1ng Real-time PCR<\/strong>"},"content":{"rendered":"

1. T\u1ed5ng quan v\u1ec1 c\u00e1c x\u00e9t nghi\u1ec7m HBV<\/strong><\/h2>\n\n\n\n

Virus vi\u00eam gan B (HBV)<\/a><\/strong> l\u00e0 m\u1ed9t trong nh\u1eefng m\u1ed1i \u0111e d\u1ecda v\u1ec1 s\u1ee9c kh\u1ecfe l\u1edbn \u0111\u1ed1i v\u1edbi nh\u00e2n lo\u1ea1i hi\u1ec7n nay. T\u00ecnh tr\u1ea1ng vi\u00eam gan m\u00e3n t\u00ednh c\u00f3 th\u1ec3 d\u1eabn \u0111\u1ebfn c\u00e1c bi\u1ebfn ch\u1ee9ng nghi\u00eam tr\u1ecdng v\u1ec1 gan bao g\u1ed3m x\u01a1 gan v\u00e0 ung th\u01b0 gan t\u1eeb \u0111\u00f3 l\u00e0m t\u0103ng nguy c\u01a1 t\u1eed vong c\u1ee7a b\u1ec7nh nh\u00e2n. V\u00ec l\u00fd do \u0111\u00f3, song song v\u1edbi c\u00e1c ch\u01b0\u01a1ng tr\u00ecnh ti\u00eam ph\u00f2ng vaccine kh\u00e1ng HBV, c\u00e1c ph\u01b0\u01a1ng ph\u00e1p x\u00e9t nghi\u1ec7m c\u00f3 kh\u1ea3 n\u0103ng ph\u00e1t hi\u1ec7n s\u1ef1 x\u00e2m nhi\u1ec5m v\u00e0 t\u00ecnh tr\u1ea1ng x\u00e2m nhi\u1ec5m (c\u1ea5p t\u00ednh, m\u00e3n t\u00ednh hay th\u1ec3 \u1ea9n) c\u1ee7a HBV l\u00e0 h\u1ebft s\u1ee9c c\u1ea7n thi\u1ebft.<\/p>\n\n\n\n

\"X\u00e9t
X\u00e9t nghi\u1ec7m Vi\u00eam gan B (HBV) <\/em><\/figcaption><\/figure>\n\n\n\n

C\u00e1c x\u00e9t nghi\u1ec7m HBV \u0111\u01b0\u1ee3c chia th\u00e0nh 2 d\u1ea1ng ch\u00ednh g\u1ed3m x\u00e9t nghi\u1ec7m huy\u1ebft thanh h\u1ecdc (serological diagnosis) v\u00e0 x\u00e9t nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed (molecular diagnosis). <\/p>\n\n\n\n

X\u00e9t nghi\u1ec7m huy\u1ebft thanh t\u1eadp trung v\u00e0o vi\u1ec7c ph\u00e1t hi\u1ec7n c\u00e1c biomarker l\u00e0 c\u00e1c kh\u00e1ng nguy\u00ean nh\u01b0 HBsAg, HBeAg, HBcAg v\u00e0 c\u00e1c kh\u00e1ng th\u1ec3 c\u1ee7a ch\u00fang. D\u1ef1a tr\u00ean s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a c\u00e1c lo\u1ea1i kh\u00e1ng nguy\u00ean, kh\u00e1ng th\u1ec3 n\u00e0y, ta c\u00f3 th\u1ec3 ph\u00e2n bi\u1ec7t gi\u1eefa c\u00e1c tr\u01b0\u1eddng h\u1ee3p ti\u00eam vaccine HBV, nhi\u1ec5m HBV c\u1ea5p t\u00ednh, nhi\u1ec5m HBV m\u00e3n t\u00ednh ho\u1eb7c \u0111\u00e3 t\u1eebng nhi\u1ec5m HBV. <\/p>\n\n\n\n

Trong c\u00e1c lo\u1ea1i kh\u00e1ng nguy\u00ean v\u00e0 kh\u00e1ng th\u1ec3, kh\u00e1ng nguy\u00ean HBsAg l\u00e0 d\u1ea1ng biomarker \u0111\u01b0\u1ee3c ph\u00e1t hi\u1ec7n \u0111\u1ea7u ti\u00ean v\u00e0 s\u1eed d\u1ee5ng ph\u1ed5 bi\u1ebfn nh\u1ea5t trong c\u00e1c x\u00e9t nghi\u1ec7m ch\u1ea9n \u0111o\u00e1n hi\u1ec7n nay. Tuy nhi\u00ean, m\u1ed9t trong nh\u1eefng nh\u01b0\u1ee3c \u0111i\u1ec3m l\u1edbn c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p x\u00e9t nghi\u1ec7m HBsAg n\u00f3i ri\u00eang v\u00e0 c\u00e1c x\u00e9t nghi\u1ec7m huy\u1ebft thanh n\u00f3i chung l\u00e0 vi\u1ec7c ch\u00fang kh\u00f4ng c\u00f3 kh\u1ea3 n\u0103ng ph\u00e1t hi\u1ec7n c\u00e1c tr\u01b0\u1eddng h\u1ee3p nhi\u1ec5m HBV th\u1ec3 \u1ea9n (do virus mang c\u00e1c \u0111\u1ed9t bi\u1ebfn tr\u00ean v\u00f9ng epitope c\u1ee7a kh\u00e1ng nguy\u00ean ho\u1eb7c \u00edt bi\u1ec3u hi\u1ec7n kh\u00e1ng nguy\u00ean tr\u00ean b\u1ec1 m\u1eb7t v\u1ecf).<\/p>\n\n\n\n

<\/div>\n\n\n\n

2. \u1ee8ng d\u1ee5ng c\u1ee7a c\u00e1c x\u00e9t nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed \u0111\u1ed1i v\u1edbi HBV<\/strong><\/h2>\n\n\n\n

V\u1edbi t\u00ecnh tr\u1ea1ng nhi\u1ec5m HBV th\u1ec3 \u1ea9n \u0111ang t\u0103ng d\u1ea7n, x\u00e9t nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed b\u1eaft \u0111\u1ea7u \u0111\u01b0\u1ee3c \u1ee9ng d\u1ee5ng nhi\u1ec1u h\u01a1n. X\u00e9t nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed cho HBV ph\u00e1t hi\u1ec7n virus th\u00f4ng qua vi\u1ec7c \u0111\u1ecbnh l\u01b0\u1ee3ng DNA v\u00e0 x\u00e1c \u0111\u1ecbnh ki\u1ec3u gene (genotype) c\u1ee7a ch\u00fang.<\/p>\n\n\n\n

\u0110\u1ecbnh l\u01b0\u1ee3ng DNA c\u1ee7a HBV (HBV DNA) l\u00e0 ph\u01b0\u01a1ng ph\u00e1p \u0111\u00e1nh gi\u00e1 ho\u1ea1t \u0111\u1ed9ng sao ch\u00e9p v\u00e0 nh\u00e2n b\u1ea3n c\u1ee7a virus t\u1eeb \u0111\u00f3 \u0111\u00e1nh gi\u00e1 t\u00ecnh tr\u1ea1ng c\u1ee7a b\u1ec7nh nh\u00e2n. T\u1ea3i l\u01b0\u1ee3ng DNA c\u00e0ng cao \u0111\u1ed3ng ngh\u0129a v\u1edbi di\u1ec5n ti\u1ebfn b\u1ec7nh c\u00e0ng nhanh v\u00e0 kh\u1ea3 n\u0103ng bi\u1ebfn ch\u1ee9ng th\u00e0nh ung th\u01b0 gan c\u00e0ng cao.<\/p>\n\n\n\n

Ngo\u00e0i m\u1ee5c \u0111\u00edch gi\u00fap x\u00e1c \u0111\u1ecbnh giai \u0111o\u1ea1n b\u1ec7nh, x\u00e9t nghi\u1ec7m DNA c\u00f2n l\u00e0 m\u1ed9t ti\u00eau ch\u00ed \u0111\u00e1nh gi\u00e1 \u0111\u1ec3 \u0111\u01b0a ra li\u1ec7u ph\u00e1p \u0111i\u1ec1u tr\u1ecb ph\u00f9 h\u1ee3p v\u1edbi t\u00ecnh tr\u1ea1ng c\u1ee7a b\u1ec7nh nh\u00e2n v\u00e0 \u0111\u1ed3ng th\u1eddi l\u00e0 \u0111\u00e1nh gi\u00e1 hi\u1ec7u qu\u1ea3 \u0111i\u1ec1u tr\u1ecb c\u1ee7a li\u1ec7u ph\u00e1p.<\/p>\n\n\n\n

<\/div>\n\n\n\n

3. C\u00e1c ph\u01b0\u01a1ng ph\u00e1p x\u00e9t nghi\u1ec7m HBV DNA<\/strong><\/h2>\n\n\n\n

Ph\u01b0\u01a1ng ph\u00e1p x\u00e9t nghi\u1ec7m DNA r\u1ea5t \u0111a d\u1ea1ng, c\u00f3 th\u1ec3 chia th\u00e0nh 2 d\u1ea1ng ch\u00ednh l\u00e0 d\u1ea1ng tr\u1ef1c ti\u1ebfp v\u00e0 d\u1ea1ng gi\u00e1n ti\u1ebfp. <\/p>\n\n\n\n

\u25ba Ph\u01b0\u01a1ng ph\u00e1p d\u1ea1ng tr\u1ef1c ti\u1ebfp<\/strong> nh\u01b0 ph\u01b0\u01a1ng ph\u00e1p lai (hybridization assay) ho\u1eb7c ph\u01b0\u01a1ng ph\u00e1p DNA nh\u00e1nh (branched DNA) s\u1eed d\u1ee5ng c\u00e1c \u0111o\u1ea1n m\u1eabu d\u00f2 \u0111\u1ec3 nh\u1eadn di\u1ec7n tr\u1ef1c ti\u1ebfp DNA c\u1ee7a virus trong m\u1eabu. C\u00e1c ph\u01b0\u01a1ng ph\u00e1p n\u00e0y kh\u00e1 \u0111\u01a1n gi\u1ea3n, d\u1ec5 th\u1ef1c hi\u1ec7n v\u00e0 kh\u00f4ng \u0111\u00f2i h\u1ecfi nhi\u1ec1u thi\u1ebft b\u1ecb ph\u1ee9c t\u1ea1p; tuy nhi\u00ean \u0111\u1ed9 nh\u1ea1y c\u1ee7a c\u00e1c ph\u01b0\u01a1ng ph\u00e1p d\u1ea1ng tr\u1ef1c ti\u1ebfp kh\u00e1 th\u1ea5p.<\/p>\n\n\n\n

\u25ba Ph\u01b0\u01a1ng ph\u00e1p d\u1ea1ng gi\u00e1n ti\u1ebfp <\/strong>c\u00f3 th\u1ec3 kh\u1eafc ph\u1ee5c \u0111\u01b0\u1ee3c nh\u01b0\u1ee3c \u0111i\u1ec3m n\u00e0y b\u1eb1ng m\u1ed9t b\u01b0\u1edbc khu\u1ebfch \u0111\u1ea1i s\u1ed1 l\u01b0\u1ee3ng m\u1ed9t \u0111o\u1ea1n DNA \u0111\u1eb7c hi\u1ec7u \u1edf virus v\u00e0 sau \u0111\u00f3 ghi nh\u1eadn t\u00edn hi\u1ec7u. C\u00e1c ph\u01b0\u01a1ng ph\u00e1p khu\u1ebfch \u0111\u1ea1i DNA r\u1ea5t \u0111a d\u1ea1ng nh\u01b0 PCR, TMA, LAMP, NASBA,… C\u00e1c ph\u01b0\u01a1ng ph\u00e1p n\u00e0y ch\u1ee7 y\u1ebfu kh\u00e1c nhau v\u1ec1 c\u01a1 ch\u1ebf khu\u1ebfch \u0111\u1ea1i (lu\u00e2n nhi\u1ec7t hay \u0111\u1eb3ng nhi\u1ec7t), enzyme s\u1eed d\u1ee5ng (DNA polymerase, RNA polymerase, Reverse transcriptase) hay c\u00e1ch thi\u1ebft k\u1ebf m\u1ed3i (b\u1ea3ng 1).<\/p>\n\n\n\n

C\u00e1c ph\u01b0\u01a1ng ph\u00e1p tr\u00ean \u0111\u1ec1u c\u00f3 \u0111\u1ed9 nh\u1ea1y cao v\u00e0 \u0111\u00e3 \u0111\u01b0\u1ee3c \u1ee9ng d\u1ee5ng \u0111\u1ec3 ph\u00e1t hi\u1ec7n s\u1ef1 x\u00e2m nhi\u1ec5m c\u1ee7a nhi\u1ec1u lo\u1ea1i virus nh\u01b0 HSV, HPV hay HIV, tuy nhi\u00ean \u0111\u1ed1i v\u1edbi c\u00e1c x\u00e9t nghi\u1ec7m DNA c\u1ee7a HBV, c\u00e1c b\u1ed9 kit th\u01b0\u01a1ng m\u1ea1i hi\u1ec7n t\u1ea1i ch\u1ee7 y\u1ebfu v\u1eabn d\u1ef1a tr\u00ean ph\u01b0\u01a1ng ph\u00e1p PCR, v\u00e0 \u0111a s\u1ed1 l\u00e0 Real-time PCR<\/strong><\/a> \u0111\u1ec3 ph\u00f9 h\u1ee3p cho nhu c\u1ea7u \u0111\u1ecbnh l\u01b0\u1ee3ng.<\/p>\n\n\n\n

Method<\/strong><\/td>C\u01a1 ch\u1ebf khu\u1ebfch \u0111\u1ea1i<\/strong><\/td>Enzyme<\/strong><\/td>M\u1ed3i<\/strong><\/td><\/tr>
PCR\/ Real time PCR<\/td>Lu\u00e2n nhi\u1ec7t<\/td>DNA polymerase<\/td>\u0110\u01a1n gi\u1ea3n, \u2265 2 \u0111o\u1ea1n m\u1ed3i<\/td><\/tr>
LCR<\/td>Lu\u00e2n nhi\u1ec7t<\/td>DNA Ligase, DNA polymerase<\/td>\u0110\u01a1n gi\u1ea3n,  4 \u0111o\u1ea1n m\u1ed3i<\/td><\/tr>
TMA<\/td>\u0110\u1eb3ng nhi\u1ec7t<\/td>Reverse transcriptase, RNA polymerase<\/td>\u0110\u01a1n gi\u1ea3n, 2 \u0111o\u1ea1n m\u1ed3i<\/td><\/tr>
LAMP<\/td>\u0110\u1eb3ng nhi\u1ec7t<\/td>Bst DNA polymerase<\/td>Ph\u1ee9c t\u1ea1p, 4-6 \u0111o\u1ea1n m\u1ed3i<\/td><\/tr>
NASBA<\/td>\u0110\u1eb3ng nhi\u1ec7t<\/td>Reverse transcriptase, T7 RNA polymerase, RNAse H<\/td>\u0110\u01a1n gi\u1ea3n, 2 \u0111o\u1ea1n m\u1ed3i<\/td><\/tr><\/tbody><\/table>
B\u1ea3ng 1: <\/strong>So s\u00e1nh c\u00e1c ph\u01b0\u01a1ng ph\u00e1p khu\u1ebfch \u0111\u1ea1i DNA<\/figcaption><\/figure>\n\n\n\n

PCR: Polymerase chain reaction; LCR: Ligase chain reaction; LAMP: Loop mediated isothermal amplification; NASBA: Nucleic acid sequence based amplification; TMA: Transcription mediated amplification;<\/em><\/p>\n\n\n\n

<\/div>\n\n\n\n

4. \u0110o t\u1ea3i l\u01b0\u1ee3ng HBV b\u1eb1ng Real-time PCR<\/strong><\/h2>\n\n\n\n
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4.1. Quy tr\u00ecnh th\u1ef1c hi\u1ec7n x\u00e9t nghi\u1ec7m HBV DNA b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p Real-time PCR <\/strong><\/h3>\n\n\n\n

Theo quy \u0111\u1ecbnh c\u1ee7a B\u1ed9 Y t\u1ebf v\u1ec1 x\u00e9t nghi\u1ec7m HBV (Quy\u1ebft \u0111\u1ecbnh s\u1ed1 1868\/Q\u0110-BYT), quy tr\u00ecnh x\u00e9t nghi\u1ec7m b\u1eaft \u0111\u1ea7u b\u1eb1ng qu\u00e1 tr\u00ecnh thu m\u1eabu m\u00e1u t\u0129nh m\u1ea1ch t\u1eeb b\u1ec7nh nh\u00e2n, sau \u0111\u00f3 m\u1eabu \u0111\u01b0\u1ee3c ly t\u00e2m \u0111\u1ec3 thu l\u1ea1i huy\u1ebft thanh v\u00e0 \u0111\u01b0\u1ee3c v\u1eadn chuy\u1ec3n \u0111\u1ebfn c\u01a1 s\u1edf x\u00e9t nghi\u1ec7m \u0111\u1ec3 th\u1ef1c hi\u1ec7n t\u00e1ch chi\u1ebft DNA, \u0111\u1eb7t ph\u1ea3n \u1ee9ng PCR v\u00e0 ph\u00e2n t\u00edch k\u1ebft qu\u1ea3. Qu\u00e1 tr\u00ecnh t\u00e1ch chi\u1ebft DNA \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n nh\u1eb1m thu nh\u1eadn l\u1ea1i DNA c\u1ee7a HBV v\u00e0 \u0111\u1ed3ng th\u1eddi lo\u1ea1i b\u1ecf c\u00e1c ch\u1ea5t trong m\u1eabu huy\u1ebft thanh c\u00f3 kh\u1ea3 n\u0103ng \u1ee9c ch\u1ebf ph\u1ea3n \u1ee9ng PCR nh\u01b0 IgG, lactoferrin v\u00e0 m\u1ed9t y\u1ebfu t\u1ed1 kh\u00e1ng virus.<\/p>\n\n\n\n

S\u1ea3n ph\u1ea9m DNA sau t\u00e1ch chi\u1ebft \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng l\u00e0 m\u1ea1ch khu\u00f4n cho ph\u1ea3n \u1ee9ng PCR. Qu\u00e1 tr\u00ecnh PCR c\u0169ng c\u1ea7n ph\u00e2n t\u00edch c\u00e1c m\u1eabu ki\u1ec3m so\u00e1t ch\u1ea5t l\u01b0\u1ee3ng<\/strong><\/a> song song v\u1edbi c\u00e1c m\u1eabu th\u1eed, c\u00e1c m\u1eabu n\u00e0y bao g\u1ed3m m\u1eabu ch\u1ee9ng d\u01b0\u01a1ng (ki\u1ec3m so\u00e1t qu\u00e1 tr\u00ecnh PCR), m\u1eabu ch\u1ee9ng \u00e2m (ki\u1ec3m so\u00e1t t\u1ea1p nhi\u1ec5m) v\u00e0 m\u1eabu ki\u1ec3m so\u00e1t qu\u00e1 tr\u00ecnh t\u00e1ch chi\u1ebft (th\u00f4ng th\u01b0\u1eddng ch\u1ee9ng n\u1ed9i b\u1ed5 sung tr\u01b0\u1edbc khi t\u00e1ch chi\u1ebft). K\u1ebft qu\u1ea3 \u0111\u1ecbnh t\u00ednh c\u00f3 th\u1ec3 x\u00e1c \u0111\u1ecbnh th\u00f4ng qua gi\u00e1 tr\u1ecb Ct. Tuy nhi\u00ean, \u0111a s\u1ed1 c\u00e1c b\u1ed9 kit Real-time PCR cho x\u00e9t nghi\u1ec7m HBV l\u00e0 d\u1ea1ng kit \u0111\u1ecbnh l\u01b0\u1ee3ng t\u1ea3i l\u01b0\u1ee3ng DNA virus cho n\u00ean qu\u00e1 tr\u00ecnh ph\u00e2n t\u00edch k\u1ebft qu\u1ea3 s\u1ebd ph\u1ee9c t\u1ea1p h\u01a1n.<\/p>\n\n\n\n

\u1ede c\u00e1c b\u1ed9 kit \u0111\u1ecbnh l\u01b0\u1ee3ng nh\u01b0 B\u1ed9 kit ABT\u00ae DNA QUANTITATIVE PCR KIT (Code: SQH-001)<\/a><\/strong> s\u1ea3n xu\u1ea5t t\u1eeb ABT, m\u1eabu ch\u1ee9ng d\u01b0\u01a1ng s\u1ebd \u0111\u01b0\u1ee3c thay b\u1eb1ng m\u1ed9t d\u00e3y c\u00e1c m\u1eabu chu\u1ea9n \u0111\u00e3 bi\u1ebft tr\u01b0\u1edbc n\u1ed3ng \u0111\u1ed9 DNA. T\u1eeb k\u1ebft qu\u1ea3 ch\u1ea1y c\u1ee7a c\u00e1c m\u1eabu chu\u1ea9n n\u00e0y, ta c\u00f3 th\u1ec3 d\u1ef1ng n\u00ean m\u1ed9t \u0111\u01b0\u1eddng chu\u1ea9n bi\u1ec3u di\u1ec5n t\u01b0\u01a1ng quan gi\u1eefa s\u1ed1 l\u01b0\u1ee3ng b\u1ea3n sao DNA v\u00e0 gi\u00e1 tr\u1ecb Ct \u1edf m\u1ed7i n\u1ed3ng \u0111\u1ed9. D\u1ef1a tr\u00ean \u0111\u01b0\u1eddng chu\u1ea9n \u0111\u00e3 ch\u1ea1y, s\u1ed1 l\u01b0\u1ee3ng b\u1ea3n sao DNA c\u1ee7a virus trong m\u1eabu th\u1eed c\u00f3 th\u1ec3 t\u00ednh \u0111\u01b0\u1ee3c t\u1eeb gi\u00e1 tr\u1ecb Ct \u0111\u01b0\u1ee3c tr\u1ea3 t\u1eeb thi\u1ebft b\u1ecb Real time PCR.<\/p>\n\n\n\n

\"K\u1ebft
K\u1ebft qu\u1ea3 \u0111\u1ecbnh l\u01b0\u1ee3ng v\u00e0 \u0111\u01b0\u1eddng chu\u1ea9n <\/em><\/figcaption><\/figure>\n\n\n\n

4.2. Ph\u00e2n t\u00edch v\u00e0 \u0111\u1ecdc k\u1ebft qu\u1ea3 Real-time PCR<\/strong><\/h3>\n\n\n\n

N\u1ed3ng \u0111\u1ed9 c\u1ee7a HBV \u1edf c\u00e1c m\u1eabu d\u01b0\u01a1ng t\u00ednh \u0111\u01b0\u1ee3c t\u00ednh ho\u00e0n to\u00e0n d\u1ef1a tr\u00ean \u0111\u01b0\u1eddng chu\u1ea9n \u0111\u01b0\u1ee3c x\u00e2y d\u1ef1ng t\u1eeb c\u00e1c chu\u1ea9n \u0111\u1ecbnh l\u01b0\u1ee3ng.<\/p>\n\n\n\n

K\u1ebft qu\u1ea3 \u0111\u1ecbnh l\u01b0\u1ee3ng HBV (IU\/ml) \u0111\u01b0\u1ee3c t\u00ednh to\u00e1n tr\u00ean c\u00f4ng th\u1ee9c b\u00ean d\u01b0\u1edbi<\/strong><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

[HBV] (IU\/ml): <\/strong>N\u1ed3ng \u0111\u1ed9 HBV-DNA trong 1mL m\u1eabu.
[KQ] (IU\/ml): <\/strong>K\u1ebft qu\u1ea3 xu\u1ea5t ra t\u1eeb m\u00e1y.
V (DNA): <\/strong>Th\u1ec3 t\u00edch DNA thu \u0111\u01b0\u1ee3c sau t\u00e1ch chi\u1ebft (\u0111\u01a1n v\u1ecb \u03bcL).
V (m\u1eabu): <\/strong>Th\u1ec3 t\u00edch m\u1eabu s\u1eed d\u1ee5ng cho t\u00e1ch chi\u1ebft (\u0111\u01a1n v\u1ecb mL)<\/em>.<\/p>\n\n\n\n

<\/div>\n\n\n\n

X\u00e9t nghi\u1ec7m \u0111\u1ecbnh l\u01b0\u1ee3ng virus vi\u00eam gan B (HBV DNA) l\u00e0 m\u1ed9t ph\u1ea7n quan tr\u1ecdng trong ch\u1ea9n \u0111o\u00e1n v\u00e0 \u0111i\u1ec1u tr\u1ecb vi\u00eam gan B. Vi\u1ec7c \u0111\u1ecdc hi\u1ec3u c\u00e1c ch\u1ec9 s\u1ed1 trong x\u00e9t nghi\u1ec7m n\u00e0y c\u0169ng g\u00f3p ph\u1ea7n v\u00e0o qu\u00e1 tr\u00ecnh \u0111\u00e1nh gi\u00e1 m\u1ee9c \u0111\u1ed9 nhi\u1ec5m virus trong c\u01a1 th\u1ec3 b\u1ec7nh nh\u00e2n, t\u00ecnh tr\u1ea1ng b\u1ec7nh v\u00e0 \u0111\u00e1nh gi\u00e1 hi\u1ec7u qu\u1ea3 c\u1ee7a li\u1ec7u ph\u00e1p, \u0111\u01b0a ra ph\u00e1c \u0111\u1ed3 \u0111i\u1ec1u tr\u1ecb ph\u00f9 h\u1ee3p.<\/p>\n\n\n\n

\u0110\u1ed3ng th\u1eddi, vi\u1ec7c l\u1ef1a ch\u1ecdn gi\u1ea3i ph\u00e1p x\u00e9t nghi\u1ec7m v\u00e0 ch\u1ea9n \u0111o\u00e1n c\u0169ng l\u00e0 ch\u00eca kh\u00f3a gi\u00fap ph\u00e1t hi\u1ec7n b\u1ec7nh s\u1edbm v\u00e0 ng\u0103n ng\u1eeba hi\u1ec7u qu\u1ea3. B\u1ed9 kit ABT\u00ae DNA QUANTITATIVE PCR KIT<\/a><\/strong>  \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 ph\u00e1t hi\u1ec7n \u0111\u1ecbnh l\u01b0\u1ee3ng s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a DNA-HBV c\u00f3 trong m\u1eabu \u0111\u01b0\u1ee3c ly tr\u00edch t\u1eeb m\u1eabu huy\u1ebft thanh\/huy\u1ebft t\u01b0\u01a1ng b\u1eb1ng k\u1ef9 thu\u1eadt real-time PCR.<\/p>\n\n\n\n

\"\"
Tham kh\u1ea3o b\u1ed9 kit ABT\u00ae DNA QUANTITATIVE PCR KIT<\/a> d\u00f9ng trong \u0111\u1ecbnh l\u01b0\u1ee3ng HBV t\u1ea1i \u0111\u00e2y<\/strong><\/a>.<\/figcaption><\/figure>\n\n\n\n

5. T\u00e0i li\u1ec7u tham kh\u1ea3o<\/strong><\/h2>\n\n\n\n

1. Datta, S., Chatterjee, S., & Veer, V. (2014). Recent advances in molecular diagnostics of hepatitis B virus. World Journal of Gastroenterology: WJG<\/em>, 20<\/em>(40), 14615.<\/p>\n\n\n\n

2. Song, J. E. (2016). Diagnosis of hepatitis B. Annals of translational medicine<\/em>, 4<\/em>(18).<\/p>\n\n\n\n

3. https:\/\/thuvienphapluat.vn\/van-ban\/The-thao-Y-te\/Quyet-dinh-1868-QD-BYT-2020-ban-hanh-Huong-dan-xet-nghiem-vi-rut-viem-gan-B-C-465161.aspx<\/a><\/p>","protected":false},"excerpt":{"rendered":"

Virus vi\u00eam gan B (HBV) l\u00e0 m\u1ed9t trong nh\u1eefng m\u1ed1i \u0111e d\u1ecda v\u1ec1 s\u1ee9c kh\u1ecfe l\u1edbn \u0111\u1ed1i v\u1edbi nh\u00e2n lo\u1ea1i hi\u1ec7n nay. T\u00ecnh tr\u1ea1ng vi\u00eam gan m\u00e3n t\u00ednh c\u00f3 th\u1ec3 d\u1eabn \u0111\u1ebfn c\u00e1c bi\u1ebfn ch\u1ee9ng nghi\u00eam tr\u1ecdng v\u1ec1 gan bao g\u1ed3m x\u01a1 gan v\u00e0 ung th\u01b0 gan t\u1eeb \u0111\u00f3 l\u00e0m t\u0103ng nguy c\u01a1 t\u1eed vong c\u1ee7a b\u1ec7nh nh\u00e2n. V\u00ec l\u00fd do \u0111\u00f3, song song v\u1edbi c\u00e1c ch\u01b0\u01a1ng tr\u00ecnh ti\u00eam ph\u00f2ng vaccine kh\u00e1ng HBV, c\u00e1c ph\u01b0\u01a1ng ph\u00e1p x\u00e9t nghi\u1ec7m c\u00f3 kh\u1ea3 n\u0103ng ph\u00e1t hi\u1ec7n s\u1ef1 x\u00e2m nhi\u1ec5m v\u00e0 t\u00ecnh tr\u1ea1ng x\u00e2m nhi\u1ec5m (c\u1ea5p t\u00ednh, m\u00e3n t\u00ednh hay th\u1ec3 \u1ea9n) c\u1ee7a HBV l\u00e0 h\u1ebft s\u1ee9c c\u1ea7n thi\u1ebft.<\/p>","protected":false},"author":1,"featured_media":9808,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/admin\/"},"uagb_comment_info":1,"uagb_excerpt":"Virus vi\u00eam gan B (HBV) l\u00e0 m\u1ed9t trong nh\u1eefng m\u1ed1i \u0111e d\u1ecda v\u1ec1 s\u1ee9c kh\u1ecfe l\u1edbn \u0111\u1ed1i v\u1edbi nh\u00e2n lo\u1ea1i hi\u1ec7n nay. T\u00ecnh tr\u1ea1ng vi\u00eam gan m\u00e3n t\u00ednh c\u00f3 th\u1ec3 d\u1eabn \u0111\u1ebfn c\u00e1c bi\u1ebfn ch\u1ee9ng nghi\u00eam tr\u1ecdng v\u1ec1 gan bao g\u1ed3m x\u01a1 gan v\u00e0 ung th\u01b0 gan t\u1eeb \u0111\u00f3 l\u00e0m t\u0103ng nguy c\u01a1 t\u1eed vong…","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/7866","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=7866"}],"version-history":[{"count":10,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/7866\/revisions"}],"predecessor-version":[{"id":13751,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/7866\/revisions\/13751"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/9808"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=7866"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/categories?post=7866"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/tags?post=7866"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}