{"id":7436,"date":"2022-11-09T16:41:53","date_gmt":"2022-11-09T09:11:53","guid":{"rendered":"https:\/\/abtvn.com\/?p=7436"},"modified":"2023-04-06T14:41:21","modified_gmt":"2023-04-06T07:11:21","slug":"rpa-nguyen-ly-va-ung-dung","status":"publish","type":"post","link":"https:\/\/abtvn.com\/en\/rpa-nguyen-ly-va-ung-dung\/","title":{"rendered":"RPA – nguy\u00ean l\u00fd v\u00e0 \u1ee9ng d\u1ee5ng<\/strong>"},"content":{"rendered":"

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Trong l\u0129nh v\u1ef1c sinh h\u1ecdc ph\u1ea9n t\u1eed, vi\u1ec7c khu\u1ebfch \u0111\u1ea1i m\u1ed9t \u0111o\u1ea1n v\u1eadt ch\u1ea5t di truy\u1ec1n l\u00ean g\u1ea5p nhi\u1ec1u l\u1ea7n s\u1ebd gi\u00fap d\u1ec5 d\u00e0ng ph\u00e1t hi\u1ec7n \u0111\u01b0\u1ee3c s\u1ef1 t\u1ed3n t\u1ea1i c\u1ee7a c\u00e1c th\u1ef1c th\u1ec3 mang v\u1eadt ch\u1ea5t di truy\u1ec1n \u0111\u00f3. Khu\u1ebfch \u0111\u1ea1i chia l\u00e0m hai nguy\u00ean l\u00fd ch\u00ednh: khu\u1ebfch \u0111\u1ea1i lu\u00e2n nhi\u1ec7t v\u00e0 khu\u1ebfch \u0111\u1ea1i \u0111\u1eb3ng nhi\u1ec7t. B\u00eanh c\u1ea1nh nh\u1eefng ph\u01b0\u01a1ng ph\u00e1p khu\u1ebfch \u0111\u1ea1i lu\u00e2n nhi\u1ec7t ph\u1ed5 bi\u1ebfn hi\u1ec7n nay nh\u01b0 PCR, trong chu\u1ed7i b\u00e0i vi\u1ebft n\u00e0y, ch\u00fang t\u00f4i s\u1ebd gi\u1edbi thi\u1ec7u l\u1ea7n l\u01b0\u1ee3t c\u00e1c ph\u01b0\u01a1ng ph\u00e1p ph\u1ed5 bi\u1ebfn c\u1ee7a khu\u1ebfch \u0111\u1ea1i \u0111\u1eb3ng nhi\u1ec7t v\u00e0 nh\u1eefng \u1ee9ng d\u1ee5ng, b\u1eaft \u0111\u1ea7u l\u00e0 k\u1ef9 thu\u1eadt (RPA)<\/p>\n\n\n\n

Gi\u1edbi thi\u1ec7u k\u1ef9 thu\u1eadt khu\u1ebfch \u0111\u1ea1i gene<\/strong><\/h2>\n\n\n\n

Gene l\u00e0 c\u00e1c \u0111o\u1ea1n ch\u1ee9a c\u00e1c ph\u00e2n t\u1eed nucleic acid ch\u1ee9a th\u00f4ng tin di truy\u1ec1n c\u1ea7n thi\u1ebft \u0111\u1eb7c tr\u01b0ng cho s\u1ef1 h\u00ecnh th\u00e0nh, ph\u00e1t tri\u1ec3n v\u00e0 ho\u1ea1t \u0111\u1ed9ng c\u1ee7a m\u1ed9t c\u00e1 th\u1ec3. V\u1ec1 k\u00edch th\u01b0\u1edbc, h\u1ec7 gene n\u1eb1m trong t\u1ebf b\u00e0o v\u1edbi k\u00edch th\u01b0\u1edbc si\u00eau nh\u1ecf t\u00ednh b\u1eb1ng \u00b5m nh\u01b0ng chi\u1ec1u d\u00e0i c\u00f3 th\u1ec3 l\u00ean \u0111\u1ebfn h\u00e0ng t\u1ef7 km. V\u00ec v\u1eady vi\u1ec7c nghi\u00ean c\u1ee9u m\u1ed9t tr\u00ecnh t\u1ef1 nh\u1ea5t \u0111\u1ecbnh c\u00f3 chi\u1ec1u d\u00e0i kho\u1ea3ng v\u00e0i tr\u0103m \u0111\u1ebfn v\u00e0i ng\u00e0n trong h\u1ec7 gen l\u00e0 r\u1ea5t kh\u00f3. C\u00e1c ph\u01b0\u01a1ng ph\u00e1p nh\u00e2n b\u1ea3n gene \u0111\u01b0\u1ee3c ra \u0111\u1eddi \u0111\u1ec3 gi\u1ea3i quy\u1ebft v\u1ea5n \u0111\u1ec1 n\u00e0y, trong \u0111\u00f3 bao g\u1ed3m hai ph\u01b0\u01a1ng ph\u00e1p khu\u1ebfch \u0111\u1ea1i lu\u00e2n nhi\u1ec7t <\/strong>and khu\u1ebfch \u0111\u1ea1i \u0111\u1eb3ng nhi\u1ec7t<\/strong>. <\/p>\n\n\n\n

Ph\u01b0\u01a1ng ph\u00e1p lu\u00e2n nhi\u1ec7t<\/strong> \u0111\u01b0\u1ee3c bi\u1ebft \u0111\u1ebfn l\u00e0 PCR (Polymerase Chain Reaction). PCR hi\u1ec7n \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng r\u1ed9ng r\u00e3i trong ch\u1ea9n \u0111o\u00e1n t\u00e1c nh\u00e2 \u1edf nhi\u1ec1u \u0111\u1ed1i t\u01b0\u1ee3ng kh\u00e1c nhau. M\u1ed9t s\u1ed1 k\u1ef9 thu\u1eadt kh\u00e1c \u0111\u01b0\u1ee3c c\u1ea3i ti\u1ebfn t\u1eeb PCR truy\u1ec1n th\u1ed1ng nh\u01b0 real-time PCR<\/a><\/strong>, Reverse-transcriptase PCR<\/strong>, hot-start PCR<\/strong>, \u2026 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng v\u1edbi nhi\u1ec1u m\u1ee5c \u0111\u00edch kh\u00e1c nhau. Tuy nhi\u00ean, PCR v\u00e0 c\u00e1c k\u1ef9 thu\u1eadt lu\u00e2n nhi\u1ec7t \u0111\u1ec1u c\u00f3 \u0111i\u1ec3m chung l\u00e0 ph\u1ea3i c\u00f3 m\u00e1y ch\u1ec9nh nhi\u1ec7t \u0111\u1eaft ti\u1ec1n, h\u1ec7 th\u1ed1ng ph\u00f2ng th\u00ed nghi\u1ec7m, y\u00eau c\u1ea7u m\u1eabu \u0111\u1ea7u v\u00e0o ph\u1ea3i \u0111\u01b0\u1ee3c tinh s\u1ea1ch v\u00e0 ph\u00e2n t\u00edch k\u1ebft qu\u1ea3 qua nhi\u1ec1u b\u01b0\u1edbc.<\/strong><\/p>\n\n\n\n

\"\u1ee9ng<\/figure>\n\n\n\n

Trong khi \u0111\u00f3, c\u00e1c ph\u01b0\u01a1ng ph\u00e1p \u0111\u1eb3ng nhi\u1ec7t<\/strong> \u0111\u00e3 ra \u0111\u1eddi v\u1edbi vi\u1ec7c th\u1ef1c hi\u1ec7n ph\u1ea3n \u1ee9ng nh\u00e2n b\u1ea3n gene m\u1ee5c ti\u00eau \u1edf m\u1ed9t nhi\u1ec7t \u0111\u1ed9 m\u00e0 kh\u00f4ng c\u1ea7n d\u00f9ng m\u00e1y lu\u00e2n nhi\u1ec7t. M\u1ed9t s\u1ed1 k\u1ef9 thu\u1eadt nh\u01b0 RPA, LAMP, HDA,<\/strong> \u2026 \u0111\u00e3 cho th\u1ea5y nh\u1eefng \u01b0u \u0111i\u1ec3m c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p \u0111\u1eb3ng nhi\u1ec7t so v\u1edbi lu\u00e2n nhi\u1ec7t. <\/p>\n\n\n\n

Trong \u0111\u00f3 c\u1ee5 th\u1ec3 RPA l\u00e0 k\u1ef9 thu\u1eadt c\u00f3 \u0111i\u1ec3m m\u1ea1nh n\u1ed5i tr\u1ed9i<\/strong> khi c\u00f3 th\u1ec3 th\u1ef1c hi\u1ec7n ngo\u00e0i th\u1ef1c \u0111\u1ecba do ph\u1ea3n \u1ee9ng khu\u1ebfch \u0111\u1ea1i gene x\u1ea3y ra \u1edf nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng hay m\u00f4i tr\u01b0\u1eddng, chi ph\u00ed th\u1ea5p, th\u1eddi gian ph\u1ea3n \u1ee9ng v\u00e0 \u0111\u1ecdc k\u1ebft qu\u1ea3 nhanh ch\u00f3ng.<\/strong> M\u1eb7c d\u00f9 \u0111\u1ed9 ph\u1ee7 v\u1ec1 \u1ee9ng d\u1ee5ng th\u1ef1c t\u1ebf c\u1ee7a RPA ch\u01b0a r\u1ed9ng v\u00e0 ph\u1ed5 bi\u1ebfn nh\u01b0 PCR hay real-time PCR nh\u01b0ng k\u1ef9 thu\u1eadt n\u00e0y mang nhi\u1ec1u ti\u1ec1m n\u0103ng v\u00e0 c\u00f3 tri\u1ec3n v\u1ecdng \u0111\u1eb7c bi\u1ec7t l\u00e0 trong ch\u1ea9n \u0111o\u00e1n nhanh c\u00e1c t\u00e1c nh\u00e2n g\u00e2y b\u1ec7nh truy\u1ec1n nhi\u1ec5m \u1edf ngay t\u1ea1i n\u01a1i ph\u00e1t hi\u1ec7n nh\u1eb1m h\u1ea1n ch\u1ebf s\u1ef1 l\u00e2y lan th\u00e0nh d\u1ecbch b\u1ec7nh.<\/p>\n\n\n\n

\"Khu\u1ebfch
H\u00ecnh1: K\u1ef9 thu\u1eadt khu\u1ebfch \u0111\u1ea1i lu\u00e2n nhi\u1ec7t (h\u00ecnh b\u00ean tr\u00e1i) v\u00e0 \u0111\u1eb3ng nhi\u1ec7t (h\u00ecnh b\u00ean ph\u1ea3i).
(ngu\u1ed3n: https:\/\/www.goldbio.com)<\/em><\/figcaption><\/figure>\n\n\n\n

<\/p>\n\n\n\n

K\u1ef9 thu\u1eadt Recombinase polymerase amplification (RPA)<\/strong><\/h2>\n\n\n\n

Recombinase polymerase amplification<\/strong> (RPA)- l\u00e0 k\u1ef9 thu\u1eadt s\u1eed d\u1ee5ng recombinase v\u00e0 protein SSB (single-stranded DNA binding) \u0111\u1ec3 t\u00e1ch m\u1ea1ch \u0111\u00f4i DNA thay th\u1ebf cho b\u01b0\u1edbc bi\u1ebfn t\u00ednh d\u00f9ng nhi\u1ec7t trong PCR, sau \u0111\u00f3 k\u1ebft h\u1ee3p ho\u1ea1t t\u00ednh DNA polymerase \u0111\u1ec3 t\u1ed5ng h\u1ee3p gene. <\/p>\n\n\n\n

C\u1ee5 th\u1ec3, ph\u1ea3n \u1ee9ng c\u1ee7a RPA b\u1eaft \u0111\u1ea7u h\u00ecnh th\u00e0nh ph\u1ee9c h\u1ee3p nucleoprotein gi\u1eefa recombinase v\u00e0 m\u1ed3i. Ph\u1ee9c h\u1ee3p n\u00e0y s\u1ebd tr\u01b0\u1ee3t v\u00e0 t\u00ecm ki\u1ebfm \u0111o\u1ea1n tr\u00ecnh t\u1ef1 t\u01b0\u01a1ng \u0111\u1ed3ng tr\u00ean s\u1ee3i DNA m\u1ea1ch \u0111\u00f4i, sau \u0111\u00f3 h\u00ecnh th\u00e0nh c\u1ea5u tr\u00fac D-loop, l\u00fac n\u00e0y protein SSB s\u1ebd b\u00e1m v\u00e0 \u1ed5n \u0111\u1ecbnh c\u1ea5u tr\u00fac m\u1edf m\u1ea1ch \u0111\u00f4i. Cu\u1ed1i c\u00f9ng, DNA polymerase s\u1ebd b\u1eaft \u0111\u1ea7u t\u1ed5ng h\u1ee3p s\u1ea3n ph\u1ea9m t\u1eeb v\u1ecb tr\u00ed m\u1ed3i b\u1eaft c\u1eb7p. K\u1ebft qu\u1ea3 RPA \u0111\u01b0\u1ee3c ki\u1ec3m tra b\u1eb1ng nhi\u1ec1u c\u00e1ch bao g\u1ed3m \u0111i\u1ec7n di, que gi\u1ea5y v\u00e0 probe g\u1eafn hu\u1ef3nh quang (d\u00f9ng m\u00e1y real-time \u0111\u1ec3 \u0111\u1ecdc k\u1ebft qu\u1ea3), t\u00f9y thu\u1ed9c v\u00e0o m\u1ee5c \u0111\u00edch khu\u1ebfch \u0111\u1ea1i gene m\u00e0 s\u1eed d\u1ee5ng ph\u01b0\u01a1ng ph\u00e1p ph\u00f9 h\u1ee3p [1]. <\/p>\n\n\n\n

Nghi\u00ean c\u1ee9u cho th\u1ea5y ng\u01b0\u1ee1ng ph\u00e1t hi\u1ec7n gene c\u1ee7a RPA c\u00f3 th\u1ec3 \u0111\u1ea1t 1 copy DNA m\u1ee5c ti\u00eau (ho\u1eb7c <10 b\u1ea3n copy RNA) m\u00e0 kh\u00f4ng y\u00eau c\u1ea7u cao v\u1ec1 \u0111\u1ed9 tinh s\u1ea1ch c\u1ee7a m\u1eabu \u0111\u1ea7u v\u00e0o, c\u00f3 th\u1ec3 d\u00f9ng tr\u1ef1c ti\u1ebfp m\u1eabu m\u00f4 hay nh\u1ef1a l\u00e1 [2,3]. Th\u1eddi gian RPA ch\u1ec9 kho\u1ea3ng 10-20 ph\u00fat, to\u00e0n b\u1ed9 ph\u1ea3n \u1ee9ng x\u1ea3y ra \u1edf nhi\u1ec7t \u0111\u1ed9 kho\u1ea3ng 37 0<\/sup>C \u2013 42 0<\/sup>C [4]. C\u00f9ng v\u1edbi \u0111\u00f3, que gi\u1ea5y c\u0169ng l\u00e0 ph\u01b0\u01a1ng ph\u00e1p \u0111\u01b0\u1ee3c k\u1ebft h\u1ee3p khi l\u00e0m RPA0 th\u1ef1c \u0111\u1ecba, d\u1ef1a tr\u00ean nguy\u00ean t\u1eafc s\u1eafc k\u00fd mi\u1ec5n d\u1ecbch s\u1eed d\u1ee5ng kh\u00e1ng th\u1ec3 \u0111\u1ea7u h\u1ea1t v\u00e0ng v\u00e0 ligand biotin \u0111\u1ec3 g\u1eafn l\u1ea7n l\u01b0\u1ee3t t\u01b0\u01a1ng \u1ee9ng v\u1edbi gene m\u1ee5c ti\u00eau c\u00f3 \u0111\u1ea7u FAM v\u00e0 biotin.<\/p>\n\n\n\n

K\u1ebft qu\u1ea3 c\u00f3 th\u1ec3 \u0111\u1ecdc nhanh b\u1eb1ng m\u1eaft th\u01b0\u1eddng, n\u1ebfu d\u01b0\u01a1ng t\u00ednh th\u00ec que gi\u1ea5y s\u1ebd xu\u1ea5t hi\u1ec7n 2 v\u1ea1ch v\u00e0 \u00e2m t\u00ednh l\u00e0 m\u1ed9t v\u1ea1ch. Trong khi hai ph\u01b0\u01a1ng ph\u00e1p \u0111i\u1ec7n di v\u00e0 d\u00f9ng probe y\u00eau c\u1ea7u nhi\u1ec1u thi\u1ebft b\u1ecb ph\u00f2ng th\u00ed nghi\u1ec7m \u0111i k\u00e8m nh\u01b0 b\u1ed9 \u0111i\u1ec7n di, \u0111\u00e8n UV hay m\u00e1y real-time. Ngo\u00e0i nh\u1eefng \u01b0u \u0111i\u1ec3m v\u1ec1 th\u1ef1c hi\u1ec7n ph\u1ea3n \u1ee9ng v\u00e0 \u0111\u1ecdc k\u1ebft qu\u1ea3, c\u00e1c th\u00e0nh ph\u1ea7n RPA th\u01b0\u1eddng \u0111\u01b0\u1ee3c tr\u1eef \u1edf d\u1ea1ng \u0111\u00f4ng kh\u00f4, ti\u1ec7n l\u1ee3i cho vi\u1ec7c s\u1eed d\u1ee5ng v\u1eadn chuy\u1ec3n v\u00e0 b\u1ea3o qu\u1ea3n. Nh\u1eefng \u0111i\u1ec1u n\u00e0y gi\u00fap RPA c\u00f3 kh\u1ea3 n\u0103ng tr\u1edf th\u00e0nh ph\u01b0\u01a1ng ph\u00e1p \u0111\u01b0\u1ee3c l\u1ef1a ch\u1ecdn h\u00e0ng \u0111\u1ea7u khi ti\u1ebfn h\u00e0nh ph\u1ea3n \u1ee9ng t\u1ea1i n\u01a1i l\u1ea5y m\u1eabu.<\/p>\n\n\n\n

\"Nguy\u00ean
H\u00ecnh 2: K\u1ef9 thu\u1eadt RPA (ngu\u1ed3n: https:\/\/international.neb.com\/<\/a>; https:\/\/2ncbio.co.kr\/)<\/em>. Hi\u1ec7n nay recombinase ch\u1ee7 y\u1ebfu \u0111\u01b0\u1ee3c th\u01b0\u01a1ng m\u1ea1i h\u00f3a t\u1eeb Escherichia coli (RecA) hay T4<\/sub>\u00a0UvsX. Protein T4<\/sub>\u00a0UvsY h\u1ed7 tr\u1ee3 s\u1ef1 h\u00ecnh th\u00e0nh ph\u1ee9c h\u1ee3p nucleoprotein v\u00e0 Carbowax20 (CW20) gi\u00fap t\u0103ng c\u01b0\u1eddng s\u1ef1 t\u01b0\u01a1ng t\u00e1c v\u1edbi DNA, thay cho polyethylene glycol. B\u00ean c\u1ea1nh \u0111\u00f3, recombinase ch\u1ee9a v\u1ecb tr\u00ed g\u1eafn ATP trong trung t\u00e2m ho\u1ea1t \u0111\u1ed9ng n\u00ean c\u00f3 kh\u1ea3 n\u0103ng chuy\u1ec3n h\u00f3a ph\u00e2n t\u1eed n\u00e0y t\u1ea1o n\u0103ng l\u01b0\u1ee3ng cho ph\u1ea3n \u1ee9ng. G32P \u0111\u00f3ng vai tr\u00f2 thay cho SSB, trong khi Bsu polymerase t\u1ed5ng h\u1ee3p s\u1ea3n ph\u1ea9m.<\/em><\/figcaption><\/figure>\n\n\n\n

H\u1ea7u h\u1ebft c\u00e1c kit th\u01b0\u01a1ng m\u1ea1i hi\u1ec7n nay \u0111\u1ec1u s\u1eed d\u1ee5ng recombinase RecA t\u1eeb\u00a0 v\u00e0\u00a0Sau<\/em>\u00a0DNA polymerase t\u1eeb\u00a0Staphylococcus aureus<\/em>. B\u00ean c\u1ea1nh \u0111\u00f3, protein T4<\/sub>\u00a0UvsY h\u1ed7 tr\u1ee3 s\u1ef1 h\u00ecnh th\u00e0nh ph\u1ee9c h\u1ee3p nucleoprotein, polyethylene glycol l\u00e0 dung m\u00f4i nh\u1eb1m t\u0103ng c\u01b0\u1eddng kh\u1ea3 n\u0103ng t\u01b0\u01a1ng t\u00e1c gi\u1eefa c\u00e1c th\u00e0nh ph\u1ea7n ph\u1ea3n \u1ee9ng v\u1edbi ph\u00e2n t\u1eed ADN. Enzyme creatine kinase x\u00fac t\u00e1c qu\u00e1 tr\u00ecnh t\u1ea1o ATP t\u1eeb phosphocreatine \u0111\u1ec3 cung c\u1ea5p n\u0103ng l\u01b0\u1ee3ng cho ho\u1ea1t \u0111\u1ed9ng c\u1ee7a c\u00e1c enzyme kh\u00e1c. \u0110\u1ebfn nay, m\u1ed9t s\u1ed1 h\u1ee3p ch\u1ea5t c\u1ea7n thi\u1ebft c\u0169ng \u0111\u01b0\u1ee3c b\u1ed5 sung v\u00e0o kit nh\u1eb1m t\u1ed1i \u01b0u h\u00f3a t\u00ednh \u0111\u1eb7c hi\u1ec7u v\u00e0 kh\u1ea3 n\u0103ng nh\u00e2n \u0111o\u1ea1n tr\u00ecnh t\u1ef1 m\u1ee5c ti\u00eau c\u1ee7a ph\u1ea3n \u1ee9ng RPA. <\/p>\n\n\n\n

<\/p>\n\n\n\n

Nghi\u00ean c\u1ee9u v\u00e0 \u1ee9ng d\u1ee5ng RPA<\/strong><\/h2>\n\n\n\n

Tr\u00ean th\u1ebf gi\u1edbi hi\u1ec7n nay, c\u00e1c c\u00f4ng b\u1ed1 v\u1ec1 \u1ee9ng d\u1ee5ng RPA c\u0169ng \u0111\u00e3 \u0111\u01b0\u1ee3c ghi nh\u1eadn. Theo \u0111\u00f3, nh\u00e0 virus h\u1ecdc Ahmed Abd El Wahed \u0111\u00e3 ch\u1ebf t\u1ea1o ra chi\u1ebfc vali nh\u1ecf g\u1ecdn ch\u1ee9a to\u00e0n b\u1ed9 nh\u1eefng th\u1ee9 c\u1ea7n thi\u1ebft cho RPA \u0111\u1ec3 ph\u00e2n t\u00edch DNA c\u1ee7a virus Ebola ngay t\u1ea1i ch\u1ed7 [5]. K\u1ef9 thu\u1eadt n\u00e0y \u0111\u00e3 khu\u1ebfch \u0111\u1ea1i DNA m\u1ee5c ti\u00eau ch\u1ec9 trong v\u00f2ng m\u01b0\u1eddi ph\u00fat, ho\u1ea1t \u0111\u1ed9ng \u1edf nhi\u1ec7t \u0111\u1ed9 37 0<\/sup>C v\u00e0 kh\u00f4ng c\u1ea7n l\u00e0m thi\u1ebft b\u1ecb m\u00e1t trong qu\u00e1 tr\u00ecnh v\u1eadn chuy\u1ec3n. B\u00ean c\u1ea1nh \u0111\u00f3, RPA c\u00f3 th\u1ec3 ki\u1ec3m tra c\u00e1c m\u1eabu m\u00e1u c\u1ee7a 8 lo\u1ea1i virus kh\u00e1c nhau t\u1eeb 3 d\u00f2ng Ebola – \u0111i\u1ec1u m\u00e0 c\u00e1c kit test nhanh kh\u00f4ng \u0111\u00e1p \u1ee9ng \u0111\u01b0\u1ee3c. <\/p>\n\n\n\n

Ngo\u00e0i ra, m\u1ed9t s\u1ed1 t\u00e1c nh\u00e2n kh\u00e1c nh\u01b0 virus ASF g\u00e2y d\u1ecbch t\u1ea3 l\u1ee3n ch\u00e2u Phi hay Adenovirus g\u00e2y nhi\u1ec1u b\u1ec7nh l\u00fd \u1edf ng\u01b0\u1eddi c\u0169ng \u0111\u00e3 ghi nh\u1eadn c\u00f3 th\u1ec3 ph\u00e1t hi\u1ec7n b\u1eb1ng RPA [6,7]. C\u00e1c nghi\u00ean c\u1ee9u m\u1edbi \u0111\u00e2y c\u0169ng \u0111\u00e3 \u1ee9ng d\u1ee5ng RPA trong ch\u1ea9n \u0111o\u00e1n nhanh SARS-CoV-2 nh\u01b0 s\u1eed d\u1ee5ng h\u1ec7 th\u1ed1ng vi gi\u1ecdt [8] hay kh\u1ea9u trang ph\u00e1t hi\u1ec7n SARS-CoV-2 b\u1eb1ng c\u00f4ng ngh\u1ec7 RT-RPA k\u1ebft h\u1ee3p CRISPR-Cas12a [9]. <\/p>\n\n\n\n

Nh\u01b0 v\u1eady, RPA hi\u1ec7n \u0111ang \u0111\u01b0\u1ee3c nghi\u00ean c\u1ee9u nhi\u1ec1u trong s\u00e0ng l\u1ecdc nhanh ch\u00f3ng v\u00e0 h\u1ea1n ch\u1ebf s\u1ef1 l\u00e2y lan nhanh ch\u00f3ng c\u1ee7a t\u00e1c nh\u00e2n g\u00e2y b\u1ec7nh, \u0111\u1eb7c bi\u1ec7t l\u00e0 b\u1ec7nh truy\u1ec1n nhi\u1ec3m do virus. M\u1eb7c d\u00f9 v\u1eady, b\u00ean c\u1ea1nh nh\u1eefng \u01b0u \u0111i\u1ec3m, RPA c\u0169ng c\u00f3 m\u1eb7t h\u1ea1n ch\u1ebf, v\u00ec s\u1eed d\u1ee5ng m\u1ed9t nhi\u1ec7t \u0111\u1ed9 cho ph\u1ea3n \u1ee9ng n\u00ean r\u1ea5t kh\u00f3 t\u1ed1i \u01b0u ph\u1ea3n \u1ee9ng v\u00e0 kh\u00f3 ki\u1ec3m so\u00e1t c\u00e1c s\u1ea3n ph\u1ea9m kh\u00f4ng \u0111\u1eb7c hi\u1ec7u. Do v\u1eady, k\u1ef9 thu\u1eadt real-time PCR hay PCR c\u1ea3i ti\u1ebfn kh\u00e1c nh\u01b0 digital PCR v\u1eabn \u0111ang chi\u1ebfm \u01b0u th\u1ebf v\u00e0 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng ph\u1ed5 bi\u1ebfn, \u0111\u1eb7c bi\u1ec7t l\u00e0 trong ch\u1ea9n \u0111o\u00e1n hay \u0111\u1ecbnh l\u01b0\u1ee3ng t\u00e1c nh\u00e2n g\u00e2y b\u1ec7nh.<\/p>\n\n\n\n

\"\u1ee9ng
H\u00ecnh2: M\u1ed9t s\u1ed1 nghi\u00ean c\u1ee9u v\u00e0 \u1ee9ng d\u1ee5ng c\u1ee7a RPA. (a)B\u1ed9 ph\u1ea3n \u1ee9ng RPA di \u0111\u1ed9ng (https:\/\/www.sciencedaily.com\/<\/a>). (b)Kh\u1ea9u trang test nhanh SARS-Cov-2 (https:\/\/www.news-medical.net\/).(c)H\u1ec7 th\u1ed1ng vi gi\u1ecdt RPA (Zhou et al., 2022)<\/figcaption><\/figure>\n\n\n\n

\u1ede Vi\u1ec7t Nam, RPA ch\u01b0a ph\u1ed5 bi\u1ebfn. H\u1ea7u h\u1ebft, vi\u1ec7c ch\u1ea9n \u0111o\u00e1n, x\u00e9t nghi\u1ec7m c\u00e1c t\u00e1c nh\u00e2n g\u00e2y b\u1ec7nh hi\u1ec7n nay v\u1eabn s\u1eed d\u1ee5ng kit real-time PCR. RPA m\u1edbi ch\u1ec9 \u0111\u01b0\u1ee3c nghi\u00ean c\u1ee9u tr\u00ean m\u1ed9t s\u1ed1 lo\u00e0i nh\u01b0 Listeria monocytogenes<\/em> g\u00e2y ng\u1ed9 \u0111\u1ed9c th\u1ef1c ph\u1ea9m [10] hay Plasmodium falciparum<\/em> and Plasmodium vivax<\/em> g\u00e2y b\u1ec7nh s\u1ed1t r\u00e9t [11]. D\u00f9 v\u1eady, v\u1edbi nhi\u1ec1u \u01b0u \u0111i\u1ec3m ph\u00f9 h\u1ee3p \u1ee9ng d\u1ee5ng \u1edf th\u1ef1c \u0111\u1ecba, k\u1ef9 thu\u1eadt RPA v\u1eabn \u0111\u1ea7y tri\u1ec3n v\u1ecdng c\u00f3 th\u1ec3 tr\u1edf th\u00e0nh m\u1ed9t trong nh\u1eefng ph\u01b0\u01a1ng ph\u00e1p ch\u1ea9n \u0111o\u00e1n c\u00e1c t\u00e1c nh\u00e2n g\u00e2y b\u1ec7nh m\u1ed9t c\u00e1ch hi\u1ec7u qu\u1ea3 trong th\u1eddi gian t\u1edbi.<\/p>","protected":false},"excerpt":{"rendered":"

Trong l\u0129nh v\u1ef1c sinh h\u1ecdc ph\u1ea9n t\u1eed, vi\u1ec7c khu\u1ebfch \u0111\u1ea1i m\u1ed9t \u0111o\u1ea1n v\u1eadt ch\u1ea5t di truy\u1ec1n l\u00ean g\u1ea5p nhi\u1ec1u l\u1ea7n s\u1ebd gi\u00fap d\u1ec5 d\u00e0ng ph\u00e1t hi\u1ec7n \u0111\u01b0\u1ee3c s\u1ef1 t\u1ed3n t\u1ea1i c\u1ee7a c\u00e1c th\u1ef1c th\u1ec3 mang v\u1eadt ch\u1ea5t di truy\u1ec1n \u0111\u00f3. Khu\u1ebfch \u0111\u1ea1i chia l\u00e0m hai nguy\u00ean l\u00fd ch\u00ednh: khu\u1ebfch \u0111\u1ea1i lu\u00e2n nhi\u1ec7t v\u00e0 khu\u1ebfch \u0111\u1ea1i […]<\/p>","protected":false},"author":1,"featured_media":7442,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[267,228],"tags":[],"class_list":["post-7436","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-sinh-hoc-phan-tu-bai-viet","category-bai-viet"],"uagb_featured_image_src":{"full":["https:\/\/abtvn.com\/wp-content\/uploads\/2022\/11\/image-3.png",1024,512,false],"thumbnail":["https:\/\/abtvn.com\/wp-content\/uploads\/2022\/11\/image-3-150x150.png",150,150,true],"medium":["https:\/\/abtvn.com\/wp-content\/uploads\/2022\/11\/image-3-300x150.png",300,150,true],"medium_large":["https:\/\/abtvn.com\/wp-content\/uploads\/2022\/11\/image-3-768x384.png",768,384,true],"large":["https:\/\/abtvn.com\/wp-content\/uploads\/2022\/11\/image-3.png",1024,512,false],"1536x1536":["https:\/\/abtvn.com\/wp-content\/uploads\/2022\/11\/image-3.png",1024,512,false],"2048x2048":["https:\/\/abtvn.com\/wp-content\/uploads\/2022\/11\/image-3.png",1024,512,false],"trp-custom-language-flag":["https:\/\/abtvn.com\/wp-content\/uploads\/2022\/11\/image-3-18x9.png",18,9,true],"woocommerce_thumbnail":["https:\/\/abtvn.com\/wp-content\/uploads\/2022\/11\/image-3.png",1024,512,false],"woocommerce_single":["https:\/\/abtvn.com\/wp-content\/uploads\/2022\/11\/image-3.png",1024,512,false],"woocommerce_gallery_thumbnail":["https:\/\/abtvn.com\/wp-content\/uploads\/2022\/11\/image-3-100x100.png",100,100,true]},"uagb_author_info":{"display_name":"ABT Team","author_link":"https:\/\/abtvn.com\/en\/author\/admin\/"},"uagb_comment_info":0,"uagb_excerpt":"Trong l\u0129nh v\u1ef1c sinh h\u1ecdc ph\u1ea9n t\u1eed, vi\u1ec7c khu\u1ebfch \u0111\u1ea1i m\u1ed9t \u0111o\u1ea1n v\u1eadt ch\u1ea5t di truy\u1ec1n l\u00ean g\u1ea5p nhi\u1ec1u l\u1ea7n s\u1ebd gi\u00fap d\u1ec5 d\u00e0ng ph\u00e1t hi\u1ec7n \u0111\u01b0\u1ee3c s\u1ef1 t\u1ed3n t\u1ea1i c\u1ee7a c\u00e1c th\u1ef1c th\u1ec3 mang v\u1eadt ch\u1ea5t di truy\u1ec1n \u0111\u00f3. Khu\u1ebfch \u0111\u1ea1i chia l\u00e0m hai nguy\u00ean l\u00fd ch\u00ednh: khu\u1ebfch \u0111\u1ea1i lu\u00e2n nhi\u1ec7t v\u00e0 khu\u1ebfch \u0111\u1ea1i…","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/7436","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=7436"}],"version-history":[{"count":3,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/7436\/revisions"}],"predecessor-version":[{"id":7856,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/7436\/revisions\/7856"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/7442"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=7436"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/categories?post=7436"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/tags?post=7436"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}