{"id":14399,"date":"2026-02-23T21:59:42","date_gmt":"2026-02-23T14:29:42","guid":{"rendered":"https:\/\/abtvn.com\/?p=14399"},"modified":"2026-05-04T09:31:18","modified_gmt":"2026-05-04T02:01:18","slug":"toi-uu-phan-ung-pcr-ky-thuat-so-vi-giot-ddpcr-cho-phat-hien-ehp-va-ahpnd","status":"publish","type":"post","link":"https:\/\/abtvn.com\/en\/toi-uu-phan-ung-pcr-ky-thuat-so-vi-giot-ddpcr-cho-phat-hien-ehp-va-ahpnd\/","title":{"rendered":"<strong>T\u1ed1i \u01b0u ph\u1ea3n \u1ee9ng PCR k\u1ef9 thu\u1eadt s\u1ed1 vi gi\u1ecdt (ddPCR) cho ph\u00e1t hi\u1ec7n EHP v\u00e0 AHPND<\/strong>"},"content":{"rendered":"<p class=\"wp-block-paragraph\"><strong>PCR k\u1ef9 thu\u1eadt s\u1ed1 vi gi\u1ecdt (droplet digital PCR \u2013 ddPCR)<\/strong> \u0111\u01b0\u1ee3c xem l\u00e0 k\u1ef9 thu\u1eadt c\u00f3 \u0111\u1ed9 nh\u1ea1y v\u00e0 \u0111\u1ed9 ch\u00ednh x\u00e1c cao, \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng cho multiplex v\u1edbi s\u1ed1 l\u01b0\u1ee3ng m\u1ee5c ti\u00eau h\u1ea1n ch\u1ebf v\u00e0 y\u00eau c\u1ea7u t\u1ed1i \u01b0u h\u00f3a cao, ph\u00f9 h\u1ee3p \u0111\u1ec3 ph\u00e1t hi\u1ec7n \u0111\u1ed3ng th\u1eddi nhi\u1ec1u t\u00e1c nh\u00e2n g\u00e2y b\u1ec7nh trong m\u1eabu th\u1ee7y s\u1ea3n. Tuy nhi\u00ean, \u0111\u1ec3 \u0111\u1ea1t hi\u1ec7u su\u1ea5t khu\u1ebfch \u0111\u1ea1i t\u1ed1i \u01b0u v\u00e0 ph\u00e2n t\u00e1ch r\u00f5 r\u00e0ng gi\u1eefa c\u00e1c gi\u1ecdt d\u01b0\u01a1ng t\u00ednh v\u00e0 \u00e2m t\u00ednh, vi\u1ec7c t\u1ed1i \u01b0u h\u00f3a \u0111i\u1ec1u ki\u1ec7n ph\u1ea3n \u1ee9ng ddPCR l\u00e0 b\u01b0\u1edbc b\u1eaft bu\u1ed9c.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">B\u00e0i vi\u1ebft n\u00e0y h\u01b0\u1edbng d\u1eabn ph\u01b0\u01a1ng ph\u00e1p t\u1ed1i \u01b0u h\u00f3a \u0111i\u1ec1u ki\u1ec7n c\u1ee7a ph\u1ea3n \u1ee9ng ddPCR k\u00e9p nh\u1eb1m ph\u00e1t hi\u1ec7n \u0111\u1ed3ng th\u1eddi<strong> <em>has become a major concern for the shrimp farming industry <\/em>(EHP)<\/strong> &#8211; t\u00e1c nh\u00e2n g\u00e2y h\u1ed9i ch\u1ee9ng ch\u1eadm l\u1edbn v\u00e0 <strong><em>Vibrio parahaemolyticus<\/em> (VPAHPND)<\/strong> &#8211; t\u00e1c nh\u00e2n g\u00e2y b\u1ec7nh ho\u1ea1i t\u1eed gan t\u1ee5y c\u1ea5p t\u00ednh tr\u00ean t\u00f4m.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">B\u00e0i vi\u1ebft tham kh\u1ea3o k\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u c\u1ee7a nh\u00f3m t\u00e1c gi\u1ea3 Zhang v\u00e0 c\u1ed9ng s\u1ef1 (2022) tr\u00ean t\u1ea1p ch\u00ed Journal of Fish Diseases. Quy tr\u00ecnh t\u1ed1i \u01b0u ho\u00e1 ddPCR g\u1ed3m c\u00e1c b\u01b0\u1edbc tham kh\u1ea3o nh\u01b0 sau:<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" style=\"font-size:32px\"><strong>1. ddPCR trong ch\u1ea9n \u0111o\u00e1n b\u1ec7nh tr\u00ean t\u00f4m<\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Droplet digital PCR (ddPCR) l\u00e0 m\u1ed9t k\u1ef9 thu\u1eadt khu\u1ebfch \u0111\u1ea1i axit nucleic cho ph\u00e9p \u0111\u1ecbnh l\u01b0\u1ee3ng tuy\u1ec7t \u0111\u1ed1i m\u00e0 kh\u00f4ng c\u1ea7n \u0111\u01b0\u1eddng chu\u1ea9n, \u0111\u1ed3ng th\u1eddi c\u00f3 \u0111\u1ed9 nh\u1ea1y v\u00e0 \u0111\u1ed9 ch\u00ednh x\u00e1c r\u1ea5t cao.<\/p>\n\n\n\n<p class=\"has-text-align-center wp-block-paragraph\"><strong><em><span style=\"text-decoration: underline;\">\u0110\u1ecdc th\u00eam: <a href=\"https:\/\/abtvn.com\/en\/tong-quan-ve-ky-thuat-pcr-ky-thuat-so-dpcr-va-pcr-nho-giot-ddpcr\/\" data-type=\"link\" data-id=\"https:\/\/abtvn.com\/tong-quan-ve-ky-thuat-pcr-ky-thuat-so-dpcr-va-pcr-nho-giot-ddpcr\/\">PCR K\u1ef9 Thu\u1eadt S\u1ed1 (dPCR) v\u00e0 Droplet Digital PCR (ddPCR) l\u00e0 g\u00ec?<\/a><\/span><\/em><\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Sau khu\u1ebfch \u0111\u1ea1i, c\u00e1c gi\u1ecdt \u0111\u01b0\u1ee3c ph\u00e2n lo\u1ea1i:<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Gi\u1ecdt d\u01b0\u01a1ng t\u00ednh (c\u00f3 t\u00edn hi\u1ec7u hu\u1ef3nh quang)<\/li>\n\n\n\n<li>Gi\u1ecdt \u00e2m t\u00ednh (kh\u00f4ng ph\u00e1t t\u00edn hi\u1ec7u hu\u1ef3nh quang)<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">D\u1ef1a tr\u00ean ph\u00e2n b\u1ed1 Poisson, h\u1ec7 th\u1ed1ng s\u1ebd t\u00ednh to\u00e1n s\u1ed1 b\u1ea3n sao DNA m\u1ee5c ti\u00eau m\u1ed9t c\u00e1ch \u0111\u1ecbnh l\u01b0\u1ee3ng tuy\u1ec7t \u0111\u1ed1i, kh\u00f4ng c\u1ea7n \u0111\u01b0\u1eddng chu\u1ea9n.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>\u01afu \u0111i\u1ec3m c\u1ee7a ddPCR trong th\u1ee7y s\u1ea3n:<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u0110\u1ed9 \u1ed5n \u0111\u1ecbnh v\u00e0 kh\u1ea3 n\u0103ng ph\u00e1t hi\u1ec7n t\u1ed1t \u1edf n\u1ed3ng \u0111\u1ed9 th\u1ea5p<\/li>\n\n\n\n<li>\u00cdt ch\u1ecbu \u1ea3nh h\u01b0\u1edfng b\u1edfi ch\u1ea5t \u1ee9c ch\u1ebf h\u01a1n so v\u1edbi qPCR trong m\u1ed9t s\u1ed1 tr\u01b0\u1eddng h\u1ee3p<\/li>\n\n\n\n<li>Ph\u00f9 h\u1ee3p cho ph\u00e1t hi\u1ec7n \u0111\u1ed3ng th\u1eddi nhi\u1ec1u t\u00e1c nh\u00e2n<\/li>\n\n\n\n<li>Ph\u00e2n t\u00e1ch r\u00f5 r\u00e0ng gi\u1eefa t\u00edn hi\u1ec7u d\u01b0\u01a1ng v\u00e0 \u00e2m<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">Tuy nhi\u00ean, \u0111\u1ec3 \u0111\u1ea1t \u0111\u01b0\u1ee3c s\u1ef1 ph\u00e2n t\u00e1ch t\u1ed1i \u01b0u gi\u1eefa c\u00e1c gi\u1ecdt, c\u1ea7n t\u1ed1i \u01b0u c\u00e1c y\u1ebfu t\u1ed1 nh\u01b0 n\u1ed3ng \u0111\u1ed9 m\u1ed3i, \u0111\u1ea7u d\u00f2 v\u00e0 nhi\u1ec7t \u0111\u1ed9 lai, v.v.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" style=\"font-size:32px\"><strong>2. <strong>Quy tr\u00ecnh t\u1ed1i \u01b0u h\u00f3a ddPCR ph\u00e1t hi\u1ec7n EHP v\u00e0 VPAHPND<\/strong><\/strong><\/h2>\n\n\n\n<h3 class=\"wp-block-heading\" style=\"font-size:28px\"><strong><strong>2.1. L\u1ef1a ch\u1ecdn gene m\u1ee5c ti\u00eau<\/strong><\/strong><\/h3>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"wp-block-paragraph\">Ph\u1ea3n \u1ee9ng khu\u1ebfch \u0111\u1ea1i gene m\u1ee5c ti\u00eau trong quy tr\u00ecnh ddPCR tu\u00e2n theo c\u00e1c nguy\u00ean t\u1eafc t\u01b0\u01a1ng t\u1ef1 nh\u01b0 qPCR. C\u1ee5 th\u1ec3 s\u1ea3n ph\u1ea9m khu\u1ebfch \u0111\u1ea1i c\u00f3 k\u00edch th\u01b0\u1edbc t\u1eeb 60 &#8211; 200 bp, h\u00e0m l\u01b0\u1ee3ng GC t\u1eeb 40-60% v\u00e0 tr\u00e1nh c\u00e1c v\u00f9ng c\u00f3 c\u1ea5u tr\u00fac th\u1ee9 c\u1ea5p.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Hai gene m\u1ee5c ti\u00eau \u0111\u01b0\u1ee3c l\u1ef1a ch\u1ecdn g\u1ed3m:<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>EHP: Gene <em>SWP<\/em> (spore wall protein 1; GeneBank No. MH365434.1)<\/li>\n\n\n\n<li>VPAHPND: Gene <em>pirA<\/em><\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\" style=\"font-size:28px\"><strong><strong>2.2. <strong>Thi\u1ebft k\u1ebf m\u1ed3i v\u00e0 \u0111\u1ea7u d\u00f2 (probe)<\/strong><\/strong><\/strong><\/h3>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"has-text-align-center wp-block-paragraph\"><em><strong><span style=\"text-decoration: underline;\">\u0110\u1ecdc th\u00eam: <a href=\"https:\/\/abtvn.com\/en\/tieu-chuan-thiet-ke-va-dau-do-cho-real-time-pcr\/\" data-type=\"link\" data-id=\"https:\/\/abtvn.com\/tieu-chuan-thiet-ke-va-dau-do-cho-real-time-pcr\/\">Ti\u00eau chu\u1ea9n thi\u1ebft k\u1ebf m\u1ed3i v\u00e0 \u0111\u1ea7u d\u00f2.<\/a><\/span><\/strong><\/em><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Trong quy tr\u00ecnh n\u00e0y, c\u00e1c \u0111o\u1ea1n m\u1ed3i v\u00e0 \u0111\u1ea7u d\u00f2 \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf b\u1eb1ng Ph\u1ea7n m\u1ec1m Primer Express\u00ae (Phi\u00ean b\u1ea3n 3.0.1, Applied Biosystems, Foster City, CA, Hoa K\u1ef3 ) nh\u01b0 trong B\u1ea3ng 1.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong><em>B\u1ea3ng 1. Tr\u00ecnh t\u1ef1 m\u1ed3i v\u00e0 \u0111\u1ea7u d\u00f2 c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p ddPCR \u0111\u1ecbnh l\u01b0\u1ee3ng EHP v\u00e0 VPAHPND<\/em><\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img fetchpriority=\"high\" decoding=\"async\" width=\"638\" height=\"358\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2026\/03\/image-1.png\" alt=\"\" class=\"wp-image-14401\" style=\"width:549px;height:auto\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2026\/03\/image-1.png 638w, https:\/\/abtvn.com\/wp-content\/uploads\/2026\/03\/image-1-300x168.png 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2026\/03\/image-1-18x10.png 18w\" sizes=\"(max-width: 638px) 100vw, 638px\" \/><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\">T\u00ednh \u0111\u1eb7c hi\u1ec7u c\u1ee7a m\u1ed3i v\u00e0 \u0111\u1ea7u d\u00f2 \u0111\u01b0\u1ee3c ki\u1ec3m tra b\u1eb1ng BLAST <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/tools\/primer-blast\/index.cgi\" target=\"_blank\" rel=\"noopener\">https:\/\/www.ncbi.nlm.nih.gov\/tools\/primer-blast\/index.cgi<\/a><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">C\u1ea5u tr\u00fac th\u1ee9 c\u1ea5p c\u1ee7a m\u1ed3i v\u00e0 \u0111\u1ea7u d\u00f2 nh\u01b0 c\u1ea5u tr\u00fac k\u1eb9p t\u00f3c hairpin ho\u1eb7c dimer \u0111\u01b0\u1ee3c ki\u1ec3m tra b\u1eb1ng ph\u1ea7n m\u1ec1m Oligo Analyser (<a href=\"https:\/\/www.idtdna.com\/pages\/tools\/oligoanalyzer\" target=\"_blank\" rel=\"noopener\">https:\/\/www.idtdna.com\/pages\/tools\/oligoanalyzer<\/a>)<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u0110\u1ea7u d\u00f2 ph\u00e1t hi\u1ec7n t\u00e1c nh\u00e2n EHP \u0111\u01b0\u1ee3c g\u1eafn v\u1edbi ch\u1ea5t ph\u00e1t hu\u1ef3nh quang (reporter) l\u00e0 FAM v\u00e0 ch\u1ea5t h\u1ea5p ph\u1ee5 hu\u1ef3nh quang (quencher) l\u00e0 MGB. Trong khi \u0111\u1ea7u d\u00f2 ph\u00e1t hi\u1ec7n t\u00e1c nh\u00e2n VPAHPND \u0111\u01b0\u1ee3c g\u1eafn ch\u1ea5t ph\u00e1t hu\u1ef3nh quang l\u00e0 VIC v\u00e0 ch\u1ea5t h\u1ea5p ph\u1ee5 hu\u1ef3nh quang l\u00e0 MGB. <\/p>\n\n\n\n<p class=\"has-text-align-center wp-block-paragraph\"><em><strong><span style=\"text-decoration: underline;\">\u0110\u1ecdc th\u00eam: <a href=\"https:\/\/oligox.abtvn.com\/reporter-va-quencher-cho-probe-qpcr\/\" data-type=\"link\" data-id=\"https:\/\/oligox.abtvn.com\/reporter-va-quencher-cho-probe-qpcr\/\">C\u00e1ch l\u1ef1a ch\u1ecdn reporter v\u00e0 quencher ph\u00f9 h\u1ee3p cho \u0111\u1ea7u d\u00f2<\/a><\/span><\/strong><\/em><\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\" style=\"font-size:28px\"><strong><strong>2.3. <strong>T\u1ed1i \u01b0u h\u00f3a \u0111i\u1ec1u ki\u1ec7n ph\u1ea3n \u1ee9ng ddPCR<\/strong><\/strong><\/strong><\/h3>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"wp-block-paragraph\">Ph\u1ea3n \u1ee9ng t\u1ed1i \u01b0u ho\u00e1 \u0111i\u1ec1u ki\u1ec7n cho m\u1ed9t ph\u1ea3n \u1ee9ng vi gi\u1ecdt ddPCR \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n theo h\u01b0\u1edbng d\u1eabn c\u1ee7a nh\u00e0 s\u1ea3n xu\u1ea5t (Bio-Rad). Quy tr\u00ecnh tham kh\u1ea3o nh\u01b0 sau:<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Chu\u1ea9n b\u1ecb th\u00e0nh ph\u1ea7n ph\u1ea3n \u1ee9ng ddPCR:<\/strong> T\u1ed5ng th\u1ec3 t\u00edch 20 \u03bcl bao g\u1ed3m 10 \u03bcl 2\u00d7ddPCR&#x2122; Supermix probe (Bio-Rad), 400 \u2013 500 nM m\u1ed3i v\u00e0 200 \u2013 300 nM \u0111\u1ea7u d\u00f2 (1 \u03bcl khu\u00f4n m\u1eabu v\u00e0 b\u1ed5 sung n\u01b0\u1edbc c\u1ea5t 2 l\u1ea7n ddH<sub>2<\/sub>O l\u00ean \u0111\u1ebfn 20 \u03bcl).<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>T\u1ed1i \u01b0u ho\u00e1 n\u1ed3ng \u0111\u1ed9 m\u1ed3i v\u00e0 \u0111\u1ea7u d\u00f2:<\/strong> C\u00e1c n\u1ed3ng \u0111\u1ed9 m\u1ed3i v\u00e0 \u0111\u1ea7u d\u00f2 \u0111\u01b0\u1ee3c t\u1ed1i \u01b0u ho\u00e1 g\u1ed3m:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>M\u1ed3i 500 nM v\u00e0 \u0111\u1ea7u d\u00f2 250 nM<\/li>\n\n\n\n<li>M\u1ed3i 500 nM v\u00e0 \u0111\u1ea7u d\u00f2 300 nM<\/li>\n\n\n\n<li>M\u1ed3i 500 nM v\u00e0 \u0111\u1ea7u d\u00f2 200 nM<\/li>\n\n\n\n<li>M\u1ed3i 400 nM v\u00e0 \u0111\u1ea7u d\u00f2 200 nM<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>T\u1ea1o vi gi\u1ecdt:<\/strong> M\u00e1y t\u1ea1o gi\u1ecdt QX200&#x2122; droplet generator (Bio-Rad, USA) \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 t\u1ea1o vi gi\u1ecdt. <\/p>\n\n\n\n<p class=\"has-text-align-center wp-block-paragraph\"><strong><em>\u0110\u1ecdc th\u00eam: <a href=\"https:\/\/abtvn.com\/en\/dpcr-dua-tren-chip-va-tren-giot-hay-nhu-tuong\/\" data-type=\"link\" data-id=\"https:\/\/abtvn.com\/dpcr-dua-tren-chip-va-tren-giot-hay-nhu-tuong\/\">Quy tr\u00ecnh th\u1ef1c hi\u1ec7n PCR k\u1ef9 thu\u1eadt s\u1ed1 d\u1ef1a tr\u00ean chip v\u00e0 tr\u00ean gi\u1ecdt\/nh\u0169 t\u01b0\u01a1ng.<\/a><\/em><\/strong><\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Ph\u1ea3n \u1ee9ng khu\u1ebfch \u0111\u1ea1i c\u00e1c vi gi\u1ecdt:<\/strong> C\u00e1c gi\u1ecdt sau khi h\u00ecnh th\u00e0nh \u0111\u01b0\u1ee3c chuy\u1ec3n sang \u0111\u0129a PCR 96 gi\u1ebfng (Bio-Rad, Hoa K\u1ef3) \u0111\u1ec3 khu\u1ebfch \u0111\u1ea1i t\u00e1c nh\u00e2n m\u1ee5c ti\u00eau. Nhi\u1ec7t \u0111\u1ed9 lai (annealing temperature) c\u1ee7a m\u1ed3i \u0111\u01b0\u1ee3c t\u1ed1i \u01b0u ho\u00e1 t\u1eeb 55 \u0111\u1ebfn 62<sup>0<\/sup>C nh\u1eb1m li\u00ean k\u1ebft v\u1edbi tr\u00ecnh t\u1ef1 DNA m\u1ee5c ti\u00eau gi\u00fap t\u0103ng \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u v\u00e0 hi\u1ec7u su\u1ea5t khu\u1ebfch \u0111\u1ea1i, t\u1eeb \u0111\u00f3 t\u0103ng kh\u1ea3 n\u0103ng ph\u00e2n bi\u1ec7t gi\u1eefa c\u00e1c gi\u1ecdt d\u01b0\u01a1ng t\u00ednh v\u00e0 \u00e2m t\u00ednh.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Quy tr\u00ecnh PCR bao g\u1ed3m:<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">&#8211; 1 chu k\u1ef3: \u00a0\u00a0\u00a0\u00a0\u00a0 95\u00b0C trong 10 ph\u00fat<br>&#8211; 40 chu k\u1ef3: \u00a0\u00a0\u00a094\u00b0C trong 30 gi\u00e2y <br>                        55 \u2013 62\u00b0C trong 60 gi\u00e2y<br>&#8211; 1 chu k\u1ef3:\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 98\u00b0C trong 10 ph\u00fat, gi\u1eef \u1edf 4\u00b0C<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>\u0110\u1ecdc t\u00edn hi\u1ec7u trong c\u00e1c gi\u1ecdt:<\/strong> Sau khi khu\u1ebfch \u0111\u1ea1i PCR, c\u00e1c gi\u1ecdt \u0111\u01b0\u1ee3c \u0111\u1ecdc ri\u00eang bi\u1ec7t b\u1eb1ng h\u1ec7 th\u1ed1ng QX200 Droplet Reader (Bio-Rad, Hoa K\u1ef3) v\u00e0 ph\u00e2n t\u00edch b\u1eb1ng ph\u1ea7n m\u1ec1m QuantaSoft&#x2122; (Bio-Rad, Hoa K\u1ef3).<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\" style=\"font-size:32px\"><strong><strong>3. <\/strong>Ph\u00e2n t\u00edch k\u1ebft qu\u1ea3 t\u1ed1i \u01b0u m\u1ed3i v\u00e0 \u0111\u1ea7u d\u00f2 cho PCR k\u1ef9 thu\u1eadt s\u1ed1 vi gi\u1ecdt ddPCR<\/strong><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">Nghi\u00ean c\u1ee9u c\u1ee5 th\u1ec3 c\u1ee7a Zhang v\u00e0 c\u1ed9ng s\u1ef1 (2022) tr\u00ecnh b\u00e0y k\u1ebft qu\u1ea3 t\u1ed1i \u01b0u h\u00f3a n\u1ed3ng \u0111\u1ed9 m\u1ed3i v\u00e0 \u0111\u1ea7u d\u00f2 cho ph\u01b0\u01a1ng ph\u00e1p PCR k\u1ef9 thu\u1eadt s\u1ed1 vi gi\u1ecdt (ddPCR) \u0111\u1ed1i v\u1edbi hai \u0111\u1ed1i t\u01b0\u1ee3ng l\u00e0 EHP v\u00e0 VPAHPND.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">T\u1ed5ng 8 m\u1eabu (lane 1 \u0111\u1ebfn 8) v\u1edbi c\u00e1c \u0111i\u1ec1u ki\u1ec7n kh\u00e1c nhau v\u1ec1 n\u1ed3ng \u0111\u1ed9 m\u1ed3i v\u00e0 \u0111\u1ea7u d\u00f2, bao g\u1ed3m:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Lane 1 &amp; 2: 500 nM m\u1ed3i + 250 nM \u0111\u1ea7u d\u00f2 (l\u1eb7p l\u1ea1i 2 l\u1ea7n)<\/li>\n\n\n\n<li>Lane 3 &amp; 4: 500 nM m\u1ed3i + 300 nM \u0111\u1ea7u d\u00f2 (l\u1eb7p l\u1ea1i 2 l\u1ea7n)<\/li>\n\n\n\n<li>Lane 5 &amp; 6: 500 nM m\u1ed3i + 200 nM \u0111\u1ea7u d\u00f2 (l\u1eb7p l\u1ea1i 2 l\u1ea7n)<\/li>\n\n\n\n<li>Lane 7 &amp; 8: 400 nM m\u1ed3i + 200 nM \u0111\u1ea7u d\u00f2 (l\u1eb7p l\u1ea1i 2 l\u1ea7n)<\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full\"><img decoding=\"async\" width=\"646\" height=\"508\" data-src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2026\/03\/image-2.png\" alt=\"H\u00ecnh 1. T\u1ed1i \u01b0u h\u00f3a n\u1ed3ng \u0111\u1ed9 m\u1ed3i v\u00e0 \u0111\u1ea7u d\u00f2 cho ph\u01b0\u01a1ng ph\u00e1p ddPCR (a) EHP gi\u1ecdt d\u01b0\u01a1ng m\u00e0u xanh d\u01b0\u01a1ng, gi\u1ecdt \u00e2m m\u00e0u n\u00e2u. (b) VPAHPND gi\u1ecdt d\u01b0\u01a1ng m\u00e0u xanh l\u00e1, gi\u1ecdt \u00e2m m\u00e0u n\u00e2u.\" class=\"wp-image-14402 lazyload\" data-srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2026\/03\/image-2.png 646w, https:\/\/abtvn.com\/wp-content\/uploads\/2026\/03\/image-2-300x236.png 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2026\/03\/image-2-15x12.png 15w\" data-sizes=\"(max-width: 646px) 100vw, 646px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 646px; --smush-placeholder-aspect-ratio: 646\/508;\" \/><figcaption class=\"wp-element-caption\"><em><strong>Figure 1.<\/strong> T\u1ed1i \u01b0u h\u00f3a n\u1ed3ng \u0111\u1ed9 m\u1ed3i v\u00e0 \u0111\u1ea7u d\u00f2 cho ph\u01b0\u01a1ng ph\u00e1p ddPCR (a) EHP gi\u1ecdt d\u01b0\u01a1ng m\u00e0u xanh d\u01b0\u01a1ng, gi\u1ecdt \u00e2m m\u00e0u n\u00e2u. (b) VPAHPND gi\u1ecdt d\u01b0\u01a1ng m\u00e0u xanh l\u00e1, gi\u1ecdt \u00e2m m\u00e0u n\u00e2u.<\/em><\/figcaption><\/figure>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"wp-block-paragraph\">K\u1ebft qu\u1ea3 cho th\u1ea5y khi s\u1eed d\u1ee5ng n\u1ed3ng \u0111\u1ed9 500 nM m\u1ed3i v\u00e0 300 nM \u0111\u1ea7u d\u00f2 (lane 3 v\u00e0 4) cho c\u00e1c gi\u1ecdt d\u01b0\u01a1ng t\u00ednh v\u00e0 \u00e2m t\u00ednh \u0111\u01b0\u1ee3c ph\u00e2n t\u00e1ch r\u00f5 r\u00e0ng so v\u1edbi \u0111\u01b0\u1eddng ng\u01b0\u1ee1ng v\u1edbi c\u1ea3 EHP v\u00e0 VPAHPND. Nh\u01b0 v\u1eady, n\u1ed3ng \u0111\u1ed9 500 nM m\u1ed3i v\u00e0 300 nM \u0111\u1ea7u d\u00f2 l\u00e0 \u0111i\u1ec1u ki\u1ec7n t\u1ed1i \u01b0u c\u1ee7a m\u1ed3i v\u00e0 \u0111\u1ea7u d\u00f2 cho ph\u1ea3n \u1ee9ng ddPCR.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">T\u00edn hi\u1ec7u c\u1ee7a gi\u1ecdt \u00e2m t\u00ednh \u1edf VPAHPND (h\u00ecnh b) cao h\u01a1n so v\u1edbi EHP (h\u00ecnh a) do quencher MGB ho\u1ea1t \u0111\u1ed9ng hi\u1ec7u qu\u1ea3 h\u01a1n v\u1edbi thu\u1ed1c nhu\u1ed9m FAM (d\u00f9ng cho EHP) so v\u1edbi VIC (d\u00f9ng cho VPAHPND).<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\" style=\"font-size:32px\"><strong>4. Ph\u00e2n t\u00edch k\u1ebft qu\u1ea3 t\u1ed1i \u01b0u nhi\u1ec7t \u0111\u1ed9 lai cho PCR k\u1ef9 thu\u1eadt s\u1ed1 vi gi\u1ecdt ddPCR\u00a0<\/strong><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">T\u1ed1i \u01b0u h\u00f3a nhi\u1ec7t \u0111\u1ed9 lai cho hai \u0111\u1ed1i t\u01b0\u1ee3ng EHP v\u00e0 VPAHPND \u1edf nhi\u1ec7t \u0111\u1ed9 kh\u1ea3o s\u00e1t t\u1eeb 55\u00b0C (lanes 1 &amp; 2), 55.9\u00b0C (lanes 3 &amp; 4), 58.3\u00b0C (lanes 5 &amp; 6), 59\u00b0C (lanes 7 &amp; 8) v\u00e0 62.1\u00b0C (lanes 9 &amp; 10).<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full\"><img decoding=\"async\" width=\"760\" height=\"353\" data-src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2026\/03\/image-3.png\" alt=\"H\u00ecnh 2. T\u1ed1i \u01b0u h\u00f3a nhi\u1ec7t \u0111\u1ed9 lai cho ph\u01b0\u01a1ng ph\u00e1p ddPCR. (a) EHP gi\u1ecdt d\u01b0\u01a1ng m\u00e0u xanh d\u01b0\u01a1ng, gi\u1ecdt \u00e2m m\u00e0u n\u00e2u; (b) VPAHPND gi\u1ecdt d\u01b0\u01a1ng m\u00e0u xanh l\u00e1, gi\u1ecdt \u00e2m m\u00e0u n\u00e2u\" class=\"wp-image-14403 lazyload\" data-srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2026\/03\/image-3.png 760w, https:\/\/abtvn.com\/wp-content\/uploads\/2026\/03\/image-3-300x139.png 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2026\/03\/image-3-18x8.png 18w\" data-sizes=\"(max-width: 760px) 100vw, 760px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 760px; --smush-placeholder-aspect-ratio: 760\/353;\" \/><figcaption class=\"wp-element-caption\"><em><strong>Figure 2. <\/strong>T\u1ed1i \u01b0u h\u00f3a nhi\u1ec7t \u0111\u1ed9 lai cho ph\u01b0\u01a1ng ph\u00e1p ddPCR. (a) EHP gi\u1ecdt d\u01b0\u01a1ng m\u00e0u xanh d\u01b0\u01a1ng, gi\u1ecdt \u00e2m m\u00e0u n\u00e2u; (b) VPAHPND gi\u1ecdt d\u01b0\u01a1ng m\u00e0u xanh l\u00e1, gi\u1ecdt \u00e2m m\u00e0u n\u00e2u<\/em>.<\/figcaption><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\">\u0110\u1ed1i v\u1edbi EHP, khi nhi\u1ec7t \u0111\u1ed9 lai t\u0103ng d\u1ea7n th\u00ec kho\u1ea3ng c\u00e1ch gi\u1eefa c\u00e1c gi\u1ecdt d\u01b0\u01a1ng t\u00ednh v\u00e0 \u00e2m t\u00ednh d\u1ea7n thu h\u1eb9p d\u1eabn \u0111\u1ebfn s\u1ef1 ph\u00e2n t\u00e1ch t\u00edn hi\u1ec7u kh\u00f4ng r\u00f5 r\u00e0ng. \u1ede m\u1ee9c nhi\u1ec7t \u0111\u1ed9 55\u00b0C (lanes 1 v\u00e0 2), s\u1ef1 ph\u00e2n t\u00e1ch gi\u1eefa gi\u1ecdt d\u01b0\u01a1ng v\u00e0 gi\u1ecdt \u00e2m l\u00e0 r\u00f5 r\u00e0ng nh\u1ea5t cho th\u1ea5y \u0111i\u1ec1u ki\u1ec7n n\u00e0y l\u00e0 t\u1ed1i \u01b0u \u0111\u1ec3 khu\u1ebfch \u0111\u1ea1i \u0111\u1eb7c hi\u1ec7u v\u00e0 ch\u00ednh x\u00e1c t\u00e1c nh\u00e2n EHP m\u1ee5c ti\u00eau<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u0110\u1ed1i v\u1edbi VPAHPND, nhi\u1ec7t \u0111\u1ed9 lai c\u00f3 \u1ea3nh h\u01b0\u1edfng kh\u00f4ng \u0111\u00e1ng k\u1ec3 \u0111\u1ebfn s\u1ef1 ph\u00e2n t\u00e1ch gi\u1eefa c\u00e1c gi\u1ecdt d\u01b0\u01a1ng t\u00ednh v\u00e0 \u00e2m t\u00ednh, t\u00edn hi\u1ec7u v\u1eabn \u1ed5n \u0111\u1ecbnh \u1edf c\u00e1c m\u1ee9c nhi\u1ec7t \u0111\u1ed9 kh\u00e1c nhau.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Do \u0111\u00f3, trong nghi\u00ean c\u1ee9u n\u00e0y, nhi\u1ec7t \u0111\u1ed9 55\u00b0C \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh l\u00e0 nhi\u1ec7t \u0111\u1ed9 lai m\u1ed3i t\u1ed1i \u01b0u cho c\u1ea3 hai t\u00e1c nh\u00e2n \u0111\u00edch (EHP v\u00e0 VPAHPND) trong ph\u1ea3n \u1ee9ng ddPCR.<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"wp-block-paragraph\">Nh\u01b0 v\u1eady, t\u1ed1i \u01b0u h\u00f3a ph\u1ea3n \u1ee9ng <strong>PCR k\u1ef9 thu\u1eadt s\u1ed1 vi gi\u1ecdt (ddPCR)<\/strong> l\u00e0 b\u01b0\u1edbc quy\u1ebft \u0111\u1ecbnh \u0111\u1ec3 x\u00e2y d\u1ef1ng quy tr\u00ecnh ph\u00e1t hi\u1ec7n \u0111a m\u1ee5c ti\u00eau c\u00f3 \u0111\u1ed9 tin c\u1eady cao, \u0111\u1eb7c bi\u1ec7t trong ph\u1ea3n \u1ee9ng <strong>ddPCR k\u00e9p<\/strong> nh\u1eb1m ph\u00e1t hi\u1ec7n \u0111\u1ed3ng th\u1eddi <strong>Enterocytozoon hepatopenaei (EHP)<\/strong> and <strong>Vibrio parahaemolyticus g\u00e2y AHPND (VPAHPND)<\/strong>. Vi\u1ec7c hi\u1ec7u ch\u1ec9nh c\u00e1c th\u00f4ng s\u1ed1 quan tr\u1ecdng nh\u01b0 n\u1ed3ng \u0111\u1ed9 m\u1ed3i, n\u1ed3ng \u0111\u1ed9 \u0111\u1ea7u d\u00f2 v\u00e0 nhi\u1ec7t \u0111\u1ed9 lai gi\u00fap t\u0103ng hi\u1ec7u su\u1ea5t khu\u1ebfch \u0111\u1ea1i, t\u1ea1o s\u1ef1 ph\u00e2n t\u00e1ch t\u00edn hi\u1ec7u r\u00f5 r\u00e0ng gi\u1eefa gi\u1ecdt d\u01b0\u01a1ng t\u00ednh v\u00e0 \u00e2m t\u00ednh, \u0111\u1ed3ng th\u1eddi h\u1ea1n ch\u1ebf nhi\u1ec5u n\u1ec1n v\u00e0 ph\u1ea3n \u1ee9ng ch\u00e9o.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u0110\u1ed1i v\u1edbi ng\u00e0nh nu\u00f4i t\u00f4m, \u0111\u00e2y kh\u00f4ng ch\u1ec9 l\u00e0 c\u1ea3i ti\u1ebfn v\u1ec1 m\u1eb7t k\u1ef9 thu\u1eadt m\u00e0 c\u00f2n l\u00e0 c\u00f4ng c\u1ee5 gi\u00e1m s\u00e1t d\u1ecbch b\u1ec7nh c\u00f3 gi\u00e1 tr\u1ecb th\u1ef1c ti\u1ec5n cao. Quy tr\u00ecnh ddPCR k\u00e9p \u0111\u01b0\u1ee3c t\u1ed1i \u01b0u gi\u00fap ph\u00e1t hi\u1ec7n s\u1edbm nguy c\u01a1 nhi\u1ec5m <strong>EHP<\/strong> and <strong>AHPND<\/strong>, nh\u1eadn di\u1ec7n tr\u01b0\u1eddng h\u1ee3p \u0111\u1ed3ng nhi\u1ec5m, h\u1ed7 tr\u1ee3 theo d\u00f5i t\u1ea3i l\u01b0\u1ee3ng m\u1ea7m b\u1ec7nh v\u00e0 \u0111\u01b0a ra quy\u1ebft \u0111\u1ecbnh x\u1eed l\u00fd k\u1ecbp th\u1eddi. B\u00ean c\u1ea1nh \u0111\u00f3, vi\u1ec7c gi\u1ea3m s\u1ed1 l\u1ea7n l\u1eb7p l\u1ea1i, ti\u1ebft ki\u1ec7m h\u00f3a ch\u1ea5t v\u00e0 t\u1ed1i \u01b0u th\u1eddi gian ph\u00e2n t\u00edch c\u0169ng g\u00f3p ph\u1ea7n n\u00e2ng cao hi\u1ec7u qu\u1ea3 v\u1eadn h\u00e0nh ph\u00f2ng x\u00e9t nghi\u1ec7m.<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\" style=\"font-size:32px\"><strong>5. T\u00e0i li\u1ec7u tham kh\u1ea3o<\/strong><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">Zhang, H., Gong, H. Y., Cao, W. W., Que, M. Y., Ye, L., &amp; Shi, L. (2022). Duplex droplet digital PCR method for the detection of Enterocytozoon hepatopenaei and Vibrio parahaemolyticus acute hepatopancreatic necrosis disease. Journal of fish diseases, 45(6), 761\u2013769. <a href=\"https:\/\/doi.org\/10.1111\/jfd.13600\" target=\"_blank\" rel=\"noopener\">https:\/\/doi.org\/10.1111\/jfd.13600<\/a><\/p>","protected":false},"excerpt":{"rendered":"<p>PCR k\u1ef9 thu\u1eadt s\u1ed1 vi gi\u1ecdt (droplet digital PCR \u2013 ddPCR) \u0111\u01b0\u1ee3c xem l\u00e0 k\u1ef9 thu\u1eadt c\u00f3 \u0111\u1ed9 nh\u1ea1y v\u00e0 \u0111\u1ed9 ch\u00ednh x\u00e1c cao, \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng cho multiplex v\u1edbi s\u1ed1 l\u01b0\u1ee3ng m\u1ee5c ti\u00eau h\u1ea1n ch\u1ebf v\u00e0 y\u00eau c\u1ea7u t\u1ed1i \u01b0u h\u00f3a cao, ph\u00f9 h\u1ee3p \u0111\u1ec3 ph\u00e1t hi\u1ec7n \u0111\u1ed3ng th\u1eddi nhi\u1ec1u t\u00e1c nh\u00e2n g\u00e2y b\u1ec7nh [&hellip;]<\/p>","protected":false},"author":1,"featured_media":14570,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[228,267],"tags":[478,550,480,448],"class_list":["post-14399","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-bai-viet","category-sinh-hoc-phan-tu-bai-viet","tag-benh-tren-tom","tag-ddpcr","tag-pcr","tag-sinh-hoc-phan-tu"],"uagb_featured_image_src":{"full":["https:\/\/abtvn.com\/wp-content\/uploads\/2026\/02\/Banner-blog-website.webp",1920,1080,false],"thumbnail":["https:\/\/abtvn.com\/wp-content\/uploads\/2026\/02\/Banner-blog-website-150x150.webp",150,150,true],"medium":["https:\/\/abtvn.com\/wp-content\/uploads\/2026\/02\/Banner-blog-website-300x169.webp",300,169,true],"medium_large":["https:\/\/abtvn.com\/wp-content\/uploads\/2026\/02\/Banner-blog-website-768x432.webp",768,432,true],"large":["https:\/\/abtvn.com\/wp-content\/uploads\/2026\/02\/Banner-blog-website-1024x576.webp",1024,576,true],"1536x1536":["https:\/\/abtvn.com\/wp-content\/uploads\/2026\/02\/Banner-blog-website-1536x864.webp",1536,864,true],"2048x2048":["https:\/\/abtvn.com\/wp-content\/uploads\/2026\/02\/Banner-blog-website.webp",1920,1080,false],"trp-custom-language-flag":["https:\/\/abtvn.com\/wp-content\/uploads\/2026\/02\/Banner-blog-website-18x10.webp",18,10,true],"woocommerce_thumbnail":["https:\/\/abtvn.com\/wp-content\/uploads\/2026\/02\/Banner-blog-website-1707x960.webp",1707,960,true],"woocommerce_single":["https:\/\/abtvn.com\/wp-content\/uploads\/2026\/02\/Banner-blog-website.webp",1920,1080,false],"woocommerce_gallery_thumbnail":["https:\/\/abtvn.com\/wp-content\/uploads\/2026\/02\/Banner-blog-website-100x100.webp",100,100,true]},"uagb_author_info":{"display_name":"ABT Team","author_link":"https:\/\/abtvn.com\/en\/author\/admin\/"},"uagb_comment_info":0,"uagb_excerpt":"PCR k\u1ef9 thu\u1eadt s\u1ed1 vi gi\u1ecdt (droplet digital PCR \u2013 ddPCR) \u0111\u01b0\u1ee3c xem l\u00e0 k\u1ef9 thu\u1eadt c\u00f3 \u0111\u1ed9 nh\u1ea1y v\u00e0 \u0111\u1ed9 ch\u00ednh x\u00e1c cao, \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng cho multiplex v\u1edbi s\u1ed1 l\u01b0\u1ee3ng m\u1ee5c ti\u00eau h\u1ea1n ch\u1ebf v\u00e0 y\u00eau c\u1ea7u t\u1ed1i \u01b0u h\u00f3a cao, ph\u00f9 h\u1ee3p \u0111\u1ec3 ph\u00e1t hi\u1ec7n \u0111\u1ed3ng th\u1eddi nhi\u1ec1u t\u00e1c nh\u00e2n g\u00e2y b\u1ec7nh&hellip;","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/14399","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=14399"}],"version-history":[{"count":6,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/14399\/revisions"}],"predecessor-version":[{"id":14569,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/14399\/revisions\/14569"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/14570"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=14399"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/categories?post=14399"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/tags?post=14399"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}