{"id":14285,"date":"2026-01-10T14:27:30","date_gmt":"2026-01-10T06:57:30","guid":{"rendered":"https:\/\/abtvn.com\/?p=14285"},"modified":"2026-01-30T14:52:32","modified_gmt":"2026-01-30T07:22:32","slug":"do-nhay-do-dac-hieu-ddpcr-phat-hien-ehp-va-vpahpnd","status":"publish","type":"post","link":"https:\/\/abtvn.com\/en\/do-nhay-do-dac-hieu-ddpcr-phat-hien-ehp-va-vpahpnd\/","title":{"rendered":"C\u00e1ch \u0111\u00e1nh gi\u00e1 \u0111\u1ed9 nh\u1ea1y v\u00e0 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u c\u1ee7a ddPCR trong ph\u00e1t hi\u1ec7n EHP v\u00e0 VPAHPND tr\u00ean t\u00f4m<\/strong>"},"content":{"rendered":"

Trong nu\u00f4i t\u00f4m c\u00f4ng nghi\u1ec7p, EHP (has become a major concern for the shrimp farming industry<\/em>)<\/strong> g\u00e2y b\u1ec7nh ch\u1eadm l\u1edbn v\u00e0 VPAHPND (Vibrio parahaemolyticus<\/em>) <\/strong>g\u00e2y h\u1ed9i ch\u1ee9ng ho\u1ea1i t\u1eed gan t\u1ee5y c\u1ea5p l\u00e0 hai t\u00e1c nh\u00e2n g\u00e2y thi\u1ec7t h\u1ea1i nghi\u00eam tr\u1ecdng, th\u01b0\u1eddng t\u1ed3n t\u1ea1i \u1edf n\u1ed3ng \u0111\u1ed9 th\u1ea5p trong giai \u0111o\u1ea1n s\u1edbm v\u00e0 kh\u00f3 ph\u00e1t hi\u1ec7n b\u1eb1ng c\u00e1c ph\u01b0\u01a1ng ph\u00e1p truy\u1ec1n th\u1ed1ng.<\/p>\n\n\n\n

PCR k\u1ef9 thu\u1eadt s\u1ed1 vi gi\u1ecdt (droplet digital PCR \u2013 ddPCR)<\/strong> \u0111ang \u0111\u01b0\u1ee3c xem l\u00e0 c\u00f4ng ngh\u1ec7 ti\u1ec1m n\u0103ng nh\u1edd kh\u1ea3 n\u0103ng ph\u00e1t hi\u1ec7n m\u1ee5c ti\u00eau v\u1edbi \u0111\u1ed9 nh\u1ea1y v\u00e0 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u cao. B\u00e0i vi\u1ebft n\u00e0y \u0111\u00e1nh gi\u00e1 \u0111\u1ed9 nh\u1ea1y v\u00e0 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u c\u1ee7a ddPCR nh\u1eb1m ph\u00e1t hi\u1ec7n EHP v\u00e0 VPAHPND \u1edf t\u00f4m. T\u1eeb \u0111\u00f3 gi\u00fap x\u00e1c \u0111\u1ecbnh hi\u1ec7u qu\u1ea3 c\u1ee7a m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p x\u00e9t nghi\u1ec7m nh\u1eb1m n\u00e2ng cao hi\u1ec7u qu\u1ea3 ch\u1ea9n \u0111o\u00e1n d\u1ecbch b\u1ec7nh th\u1ee7y s\u1ea3n.<\/p>\n\n\n\n

B\u00e0i vi\u1ebft tham kh\u1ea3o k\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u c\u1ee7a nh\u00f3m t\u00e1c gi\u1ea3 Zhang v\u00e0 c\u1ed9ng s\u1ef1 (2022) tr\u00ean t\u1ea1p ch\u00ed Journal of Fish Diseases [1]<\/em><\/p>\n\n\n\n

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1. ddPCR l\u00e0 g\u00ec?<\/strong><\/h2>\n\n\n\n

Droplet digital PCR (ddPCR)<\/a> <\/strong>l\u00e0 k\u1ef9 thu\u1eadt PCR th\u1ebf h\u1ec7 m\u1edbi, trong \u0111\u00f3 ph\u1ea3n \u1ee9ng \u0111\u01b0\u1ee3c ph\u00e2n t\u00e1ch th\u00e0nh h\u00e0ng ch\u1ee5c ngh\u00ecn vi gi\u1ecdt, cho ph\u00e9p \u0111\u1ecbnh l\u01b0\u1ee3ng tuy\u1ec7t \u0111\u1ed1i DNA\/RNA m\u1ee5c ti\u00eau m\u00e0 kh\u00f4ng c\u1ea7n \u0111\u01b0\u1eddng chu\u1ea9n. \u0110i\u1ec1u n\u00e0y gi\u00fap ddPCR \u0111\u1eb7c bi\u1ec7t ph\u00f9 h\u1ee3p cho c\u00e1c m\u1eabu th\u1ee7y s\u1ea3n c\u00f3 n\u1ec1n ph\u1ee9c t\u1ea1p v\u00e0 n\u1ed3ng \u0111\u1ed9 m\u1ea7m b\u1ec7nh th\u1ea5p.<\/p>\n\n\n\n

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2. Ph\u01b0\u01a1ng ph\u00e1p \u0111\u00e1nh gi\u00e1 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u c\u1ee7a ddPCR<\/strong><\/h2>\n\n\n\n

Specificity <\/strong>ph\u1ea3n \u00e1nh kh\u1ea3 n\u0103ng ph\u00e1t hi\u1ec7n ch\u00ednh x\u00e1c t\u00e1c nh\u00e2n m\u1ee5c ti\u00eau m\u00e0 kh\u00f4ng ph\u00e1t hi\u1ec7n c\u00e1c tr\u00ecnh t\u1ef1 t\u01b0\u01a1ng t\u1ef1 kh\u00e1c c\u00f3 trong m\u1eabu.<\/p>\n\n\n\n

\u0110\u1ec3 \u0111\u00e1nh gi\u00e1 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p PCR k\u1ef9 thu\u1eadt s\u1ed1 vi gi\u1ecdt trong vi\u1ec7c ph\u00e1t hi\u1ec7n EHP v\u00e0 VPAHPND, m\u1eabu t\u00f4m nhi\u1ec5m c\u00e1c t\u00e1c nh\u00e2n g\u00e2y b\u1ec7nh kh\u00e1c nhau \u0111\u01b0\u1ee3c thu th\u1eadp bao g\u1ed3m:<\/p>\n\n\n\n

PC. Ch\u1ee9ng d\u01b0\u01a1ng
1. WSSV (White Spot Syndrome Virus)  – virus g\u00e2y b\u1ec7nh \u0111\u1ed1m tr\u1eafng
2. TSV (Taura Syndrome Virus) – virus g\u00e2y h\u1ed9i ch\u1ee9ng Taura
3. YHV(Yellow Head Virus) – virus g\u00e2y b\u1ec7nh \u0111\u1ea7u v\u00e0ng
4. VH (Vibrio harveyi) – vi khu\u1ea9n g\u00e2y b\u1ec7nh ph\u00e1t s\u00e1ng v\u00e0 nhi\u1ec5m tr\u00f9ng \u0111\u01b0\u1eddng ru\u1ed9t
5. DIV1 (Decapod Iridescent Virus 1) – virus DNA nguy hi\u1ec3m g\u00e2y ra b\u1ec7nh DIV1
6. VP (Vibrio parahaemolyticus) –  vi khu\u1ea9n g\u00e2y b\u1ec7nh ho\u1ea1i t\u1eed gan t\u1ee5y c\u1ea5p t\u00ednh (AHPND)
7. NHPB (Necrotizing Hepatopancreatitis Bacterium) –  vi khu\u1ea9n g\u00e2y b\u1ec7nh \u0111\u1ed1m \u0111en
8. IHHNV (Infectious Hypodermal and Hematopoietic Necrosis Virus) – virus g\u00e2y ra b\u1ec7nh ho\u1ea1i t\u1eed d\u01b0\u1edbi v\u1ecf v\u00e0 c\u01a1 quan t\u1ea1o m\u00e1u \u1edf t\u00f4m.
9. EHP (Enterocytozoon hepatopenaei) – vi b\u00e0o t\u1eed tr\u00f9ng g\u00e2y ra b\u1ec7nh c\u00f2i c\u1ecdc tr\u00ean t\u00f4m
10. VPAHPND (Vibrio parahaemolyticus) \u2013 vi khu\u1ea9n g\u00e2y b\u1ec7nh ho\u1ea1i t\u1eed gan t\u1ee5y c\u1ea5p t\u00ednh
NC.    N\u01b0\u1edbc c\u1ea5t hai l\u1ea7n –  \u0111\u1ed1i ch\u1ee9ng \u00e2m<\/p>\n\n\n\n

\"H\u00ecnh
Figure 1. <\/strong>\u0110\u1ed9 \u0111\u1eb7c hi\u1ec7u c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p ddPCR.<\/em>
(a) t\u00edn hi\u1ec7u c\u1ee7a EHP k\u00eanh Ch1 (b) t\u00edn hi\u1ec7u c\u1ee7a VPAHPND k\u00eanh Ch2. \u0110\u01b0\u1eddng m\u00e0u h\u1ed3ng ng\u01b0\u1ee1ng ph\u00e2n bi\u1ec7t d\u01b0\u01a1ng t\u00ednh v\u00e0 \u00e2m t\u00ednh.<\/em><\/figcaption><\/figure>\n\n\n\n

K\u1ebft qu\u1ea3 cho th\u1ea5y:<\/strong> ch\u1ee9ng d\u01b0\u01a1ng (PC) \u1edf c\u1ea3 2 th\u1eed nghi\u1ec7m \u0111\u1ec1u cho k\u1ebft qu\u1ea3 d\u01b0\u01a1ng t\u00ednh v\u00e0 ch\u1ee9ng \u00e2m (NC) \u0111\u1ec1u \u00e2m t\u00ednh kh\u00f4ng ghi nh\u1eadn t\u00edn hi\u1ec7u.<\/p>\n\n\n\n

M\u1eabu t\u00f4m nhi\u1ec5m EHP (h\u00ecnh 1a \u2013 Lane 9) v\u00e0 VPAHPND (h\u00ecnh 1b \u2013 Lane 10) cho k\u1ebft qu\u1ea3 d\u01b0\u01a1ng t\u00ednh r\u00f5 r\u00e0ng v\u1edbi t\u00edn hi\u1ec7u v\u01b0\u1ee3t ng\u01b0\u1ee1ng trong \u0111\u1ed3 th\u1ecb.<\/p>\n\n\n\n

C\u00e1c m\u1eabu c\u00f2n l\u1ea1i \u0111\u1ec1u kh\u00f4ng ghi nh\u1eadn\u00a0t\u00edn hi\u1ec7u cho th\u1ea5y kh\u00f4ng c\u00f3 ph\u1ea3n \u1ee9ng ch\u00e9o v\u1edbi c\u00e1c t\u00e1c nh\u00e2n kh\u00e1c nh\u01b0 WSSV, TSV, YHV, DIV1 hay IHHNV. K\u1ebft qu\u1ea3 n\u00e0y ch\u1ee9ng minh r\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p ddPCR c\u00f3 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u cao trong ph\u00e1t hi\u1ec7n EHP v\u00e0 VPAHPND tr\u00ean t\u00f4m.<\/p>\n\n\n\n

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3. Ph\u01b0\u01a1ng ph\u00e1p \u0111\u00e1nh gi\u00e1 \u0111\u1ed9 nh\u1ea1y c\u1ee7a ddPCR<\/strong><\/h2>\n\n\n\n

Analytical sensitivity <\/strong>l\u00e0 kh\u1ea3 n\u0103ng ph\u00e1t hi\u1ec7n \u0111\u01b0\u1ee3c n\u1ed3ng \u0111\u1ed9 r\u1ea5t th\u1ea5p m\u1ed9t m\u1ee5c ti\u00eau c\u00f3 trong m\u1eabu. Ch\u1ec9 s\u1ed1 n\u00e0y th\u01b0\u1eddng \u0111\u01b0\u1ee3c bi\u1ec3u th\u1ecb b\u1eb1ng gi\u1edbi h\u1ea1n ph\u00e1t hi\u1ec7n (LOD \u2013 Limit of Detection) t\u1ee9c l\u00e0 n\u1ed3ng \u0111\u1ed9 th\u1ea5p nh\u1ea5t c\u1ee7a m\u1ee5c ti\u00eau m\u00e0 x\u00e9t nghi\u1ec7m c\u00f3 th\u1ec3 ph\u00e1t hi\u1ec7n \u0111\u01b0\u1ee3c m\u1ed9t c\u00e1ch \u1ed5n \u0111\u1ecbnh, th\u01b0\u1eddng \u0111\u1ea1t t\u1ef7 l\u1ec7 \u226595% s\u1ed1 m\u1eabu.<\/p>\n\n\n\n

\u0110\u1ec3 \u0111\u00e1nh gi\u00e1 \u0111\u1ed9 nh\u1ea1y c\u1ee7a k\u1ef9 thu\u1eadt ddPCR trong ph\u00e1t hi\u1ec7n \u0111\u1ed3ng th\u1eddi EHP v\u00e0 VPAHPND, gene SWP1<\/em> c\u1ee7a EHP v\u00e0 gene pirA<\/em> c\u1ee7a VPAHPND \u0111\u01b0\u1ee3c t\u1ed5ng h\u1ee3p v\u00e0 n\u1ed1i v\u00e0o vector pUC57 t\u1ea1o th\u00e0nh hai plasmid pUC57-EHP v\u00e0 pUC57-VPAHPND.<\/p>\n\n\n\n

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C\u00e1c plasmid n\u00e0y \u0111\u01b0\u1ee3c pha lo\u00e3ng b\u1eadc 10 b\u1eb1ng dung d\u1ecbch TE 1X \u0111\u1ec3 t\u1ea1o d\u1ea3i n\u1ed3ng \u0111\u1ed9 chu\u1ea9n g\u1ed3m:<\/p>\n\n\n\n

pUC57-EHP (ch\u1ea5m xanh d\u01b0\u01a1ng)<\/strong><\/td>pUC57-VPAHPND<\/strong> (ch\u1ea5m xanh l\u00e1)<\/strong><\/td><\/tr>
2.6\u00d7104 <\/sup>copies\/ \u03bcL (Lane 1 & 2) 2.6\u00d7103 <\/sup>copies\/ \u03bcL (Lane 3 & 4) 2.6\u00d7102<\/sup>copies\/ \u03bcL (Lane 5 & 6) 2.6\u00d7101 <\/sup>copies\/ \u03bcL (Lane 7 & 8) 2.6\u00d7100 <\/sup>copies\/ \u03bcL (Lane 9 & 10) Ch\u1ee9ng \u00e2m (Lane 11 & 12)<\/td>5\u00d7104<\/sup> copies\/\u03bcL (Lane 1 & 2) 5\u00d7103<\/sup> copies\/\u03bcL (Lane 3 & 4) 5\u00d7102<\/sup> copies\/\u03bcL (Lane 5 & 6) 5\u00d7101<\/sup> copies\/\u03bcL (Lane 7 & 8) 5\u00d7100<\/sup> copies\/\u03bcL (Lane 9 & 10) Ch\u1ee9ng \u00e2m (Lane 11 & 12)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

M\u1ed7i n\u1ed3ng \u0111\u1ed9 \u0111\u01b0\u1ee3c ph\u00e2n c\u00e1ch b\u1eb1ng c\u00e1c \u0111\u01b0\u1eddng m\u00e0u v\u00e0ng v\u00e0 l\u1eb7p l\u1ea1i hai l\u1ea7n \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh gi\u1edbi h\u1ea1n ph\u00e1t hi\u1ec7n (LOD). Sau \u0111\u00f3, n\u1ed3ng \u0111\u1ed9 th\u1ea5p nh\u1ea5t c\u00f3 th\u1ec3 ph\u00e1t hi\u1ec7n \u0111\u01b0\u1ee3c \u0111\u01b0\u1ee3c l\u1eb7p l\u1ea1i 8 l\u1ea7n nh\u1eb1m x\u00e1c nh\u1eadn \u0111\u1ed9 nh\u1ea1y ph\u00e2n t\u00edch c\u1ee7a ddPCR.<\/p>\n\n\n\n

Ngo\u00e0i ra, c\u00e1c \u0111\u1ed9 pha lo\u00e3ng t\u01b0\u01a1ng t\u1ef1 c\u0169ng \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n v\u1edbi m\u1eabu DNA t\u00f4m kh\u00f4ng nhi\u1ec5m EHP v\u00e0 VPAHPND \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 xem DNA trong m\u1eabu t\u00f4m c\u00f3 \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn \u0111\u1ed9 nh\u1ea1y c\u1ee7a ddPCR hay kh\u00f4ng.<\/p>\n\n\n\n

\"H\u00ecnh
Figure 2. <\/strong>\u0110\u1ed9 nh\u1ea1y ph\u00e2n t\u00edch c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p ddPCR. (A) plasmid pha lo\u00e3ng v\u1edbi TE 1X; (B) plasmid pha lo\u00e3ng v\u1edbi DNA t\u00f4m; t\u00edn hi\u1ec7u c\u1ee7a EHP k\u00eanh Ch1: m\u00e0u xanh d\u01b0\u01a1ng; t\u00edn hi\u1ec7u c\u1ee7a VPAHPND k\u00eanh Ch2: m\u00e0u xanh l\u00e1<\/em><\/figcaption><\/figure>\n\n\n\n
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Ph\u01b0\u01a1ng ph\u00e1p ddPCR cho th\u1ea5y ng\u01b0\u1ee1ng ph\u00e1t hi\u1ec7n th\u1ea5p nh\u1ea5t c\u1ee7a EHP khi pha lo\u00e3ng v\u1edbi TE l\u00e0 2.6 copies\/\u03bcL (Lane 10) v\u00e0 khi pha lo\u00e3ng v\u1edbi DNA t\u00f4m l\u00e0 2.3 copies\/\u03bcL (Lane 10). T\u01b0\u01a1ng t\u1ef1, VPAHPND c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c ph\u00e1t hi\u1ec7n \u1edf n\u1ed3ng \u0111\u1ed9 th\u1ea5p nh\u1ea5t l\u00e0 3.7 copies\/\u03bcL khi pha v\u1edbi TE 1X v\u00e0 pha lo\u00e3ng v\u1edbi DNA t\u00f4m l\u00e0 4.6 copies\/\u03bcL. \u0110i\u1ec1u n\u00e0y ch\u1ee9ng minh ddPCR c\u00f3 \u0111\u1ed9 nh\u1ea1y cao ph\u00e1t hi\u1ec7n EHP v\u00e0 VPAHPND \u1edf n\u1ed3ng \u0111\u1ed9 th\u1ea5p, n\u1ec1n m\u1eabu kh\u00e1c nhau kh\u00f4ng \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn \u0111\u1ed9 nh\u1ea1y c\u1ee7a th\u1eed nghi\u1ec7m.<\/p>\n\n\n\n

\"H\u00ecnh
Figure 3. <\/strong>\u0110\u1ed9 nh\u1ea1y ph\u00e2n t\u00edch v\u00e0 \u0111\u01b0\u1eddng chu\u1ea9n c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p qPCR.<\/em>
(a) \u0110\u1ed9 nh\u1ea1y v\u1edbi EHP; (b) \u0110\u1ed9 nh\u1ea1y v\u1edbi VPAHPND; (c) \u0110\u01b0\u1eddng chu\u1ea9n c\u1ee7a EHP; (d) \u0110\u01b0\u1eddng chu\u1ea9n v\u1edbi VPAHPND<\/em><\/figcaption><\/figure>\n\n\n\n
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So s\u00e1nh v\u1edbi ph\u01b0\u01a1ng ph\u00e1p Real-time PCR \u0111\u1ecbnh l\u01b0\u1ee3ng, EHP ch\u1ec9 \u0111\u01b0\u1ee3c ph\u00e1t hi\u1ec7n \u1edf ng\u01b0\u1ee1ng th\u1ea5p nh\u1ea5t l\u00e0 2.6 \u00d7 10\u00b9 copies\/\u03bcL (H\u00ecnh 3a \u0111\u01b0\u1eddng m\u00e0u v\u00e0ng) v\u00e0 VPAHPND t\u1eeb 5.0 \u00d7 10\u00b9 copies\/\u03bcL tr\u1edf l\u00ean (H\u00ecnh 3b \u0111\u01b0\u1eddng m\u00e0u v\u00e0ng).<\/p>\n\n\n\n

M\u1eb7c d\u00f9 c\u00e1c ph\u1ea3n \u1ee9ng qPCR \u0111\u1ec1u c\u00f3 h\u1ec7 s\u1ed1 tuy\u1ebfn t\u00ednh (R\u00b2) > 0.999 ch\u1ee9ng t\u1ecf \u0111\u1ed9 ch\u00ednh x\u00e1c v\u00e0 tuy\u1ebfn t\u00ednh cao, tuy nhi\u00ean \u0111\u1ed9 nh\u1ea1y ph\u00e1t hi\u1ec7n c\u1ee7a qPCR v\u1eabn th\u1ea5p h\u01a1n kho\u1ea3ng 10 l\u1ea7n so v\u1edbi ddPCR \u0111\u1eb7c bi\u1ec7t \u1edf c\u00e1c n\u1ed3ng \u0111\u1ed9 c\u1ef1c th\u1ea5p.<\/p>\n\n\n\n

Kh\u00f4ng ch\u1ec9 c\u00f3 \u0111\u1ed9 nh\u1ea1y v\u00e0 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u cao, ddPCR kh\u00f4ng c\u1ea7n \u0111\u01b0\u1eddng chu\u1ea9n, \u00edt b\u1ecb \u1ea3nh h\u01b0\u1edfng b\u1edfi ch\u1ea5t \u1ee9c ch\u1ebf trong m\u1eabu v\u00e0 \u0111\u1eb7c bi\u1ec7t ph\u00f9 h\u1ee3p cho ph\u00e1t hi\u1ec7n \u0111\u1ed3ng th\u1eddi nhi\u1ec1u t\u00e1c nh\u00e2n g\u00e2y b\u1ec7nh. V\u1edbi nh\u1eefng \u01b0u \u0111i\u1ec3m v\u01b0\u1ee3t tr\u1ed9i, ddPCR \u0111ang tr\u1edf th\u00e0nh xu h\u01b0\u1edbng c\u00f4ng ngh\u1ec7 x\u00e9t nghi\u1ec7m hi\u1ec7n \u0111\u1ea1i trong ng\u00e0nh th\u1ee7y s\u1ea3n, g\u00f3p ph\u1ea7n n\u00e2ng cao hi\u1ec7u qu\u1ea3 v\u00e0 an to\u00e0n sinh h\u1ecdc trong nu\u00f4i t\u00f4m c\u00f4ng nghi\u1ec7p.<\/p>\n\n\n\n

<\/div>\n\n\n\n

K\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u cho th\u1ea5y ddPCR l\u00e0 c\u00f4ng c\u1ee5 ch\u1ea9n \u0111o\u00e1n hi\u1ec7u qu\u1ea3 trong ph\u00e1t hi\u1ec7n EHP v\u00e0 VPAHPND, v\u01b0\u1ee3t tr\u1ed9i h\u01a1n qPCR v\u1ec1 \u0111\u1ed9 nh\u1ea1y, \u0111\u1ed3ng th\u1eddi \u0111\u1ea3m b\u1ea3o \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u cao. \u0110\u00e2y l\u00e0 n\u1ec1n t\u1ea3ng quan tr\u1ecdng cho vi\u1ec7c \u1ee9ng d\u1ee5ng ddPCR trong qu\u1ea3n l\u00fd d\u1ecbch b\u1ec7nh th\u1ee7y s\u1ea3n hi\u1ec7n \u0111\u1ea1i.<\/p>\n\n\n\n

<\/div>\n\n\n\n

4. T\u00e0i li\u1ec7u tham kh\u1ea3o<\/strong><\/h2>\n\n\n\n

Zhang, H., Gong, H. Y., Cao, W. W., Que, M. Y., Ye, L., & Shi, L. (2022). Duplex droplet digital PCR method for the detection of Enterocytozoon hepatopenaei and Vibrio parahaemolyticus acute hepatopancreatic necrosis disease. Journal of fish diseases, 45(6), 761\u2013769. https:\/\/doi.org\/10.1111\/jfd.13600.<\/a><\/p>\n\n\n\n

<\/p>","protected":false},"excerpt":{"rendered":"

Ph\u00e2n t\u00edch \u0111\u1ed9 nh\u1ea1y v\u00e0 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u c\u1ee7a k\u1ef9 thu\u1eadt ddPCR trong ph\u00e1t hi\u1ec7n EHP v\u00e0 VPAHPND tr\u00ean t\u00f4m, so s\u00e1nh v\u1edbi qPCR v\u00e0 \u00fd ngh\u0129a \u1ee9ng d\u1ee5ng th\u1ef1c ti\u1ec5n.<\/p>","protected":false},"author":1,"featured_media":14301,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/admin\/"},"uagb_comment_info":0,"uagb_excerpt":"Ph\u00e2n t\u00edch \u0111\u1ed9 nh\u1ea1y v\u00e0 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u c\u1ee7a k\u1ef9 thu\u1eadt ddPCR trong ph\u00e1t hi\u1ec7n EHP v\u00e0 VPAHPND tr\u00ean t\u00f4m, so s\u00e1nh v\u1edbi qPCR v\u00e0 \u00fd ngh\u0129a \u1ee9ng d\u1ee5ng th\u1ef1c ti\u1ec5n.","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/14285","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=14285"}],"version-history":[{"count":7,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/14285\/revisions"}],"predecessor-version":[{"id":14302,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/14285\/revisions\/14302"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/14301"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=14285"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/categories?post=14285"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/tags?post=14285"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}