{"id":13435,"date":"2025-10-06T10:50:00","date_gmt":"2025-10-06T03:20:00","guid":{"rendered":"https:\/\/abtvn.com\/?p=13435"},"modified":"2025-10-24T16:50:24","modified_gmt":"2025-10-24T09:20:24","slug":"sach-ky-thuat-real-time-pcr-tu-ly-thuyet-den-thuc-hanh","status":"publish","type":"post","link":"https:\/\/abtvn.com\/en\/sach-ky-thuat-real-time-pcr-tu-ly-thuyet-den-thuc-hanh\/","title":{"rendered":"L\u1eadt m\u1edf t\u1eebng kh\u00eda c\u1ea1nh c\u1ee7a k\u1ef9 thu\u1eadt Real-time PCR v\u1edbi quy\u1ec3n s\u00e1ch \u0111\u01b0\u1ee3c bi\u00ean so\u1ea1n b\u1edfi ABT"},"content":{"rendered":"

Trong b\u1ed1i c\u1ea3nh c\u00e1c k\u1ef9 thu\u1eadt sinh h\u1ecdc ph\u00e2n t\u1eed ng\u00e0y c\u00e0ng ph\u00e1t tri\u1ec3n, PCR v\u00e0 Real-time PCR \u0111ang tr\u1edf th\u00e0nh c\u00f4ng c\u1ee5 kh\u00f4ng th\u1ec3 thi\u1ebfu trong nghi\u00ean c\u1ee9u v\u00e0 ch\u1ea9n \u0111o\u00e1n. Cu\u1ed1n s\u00e1ch\u201cK\u1ef9 thu\u1eadt PCR v\u00e0 Real-time PCR: T\u1eeb l\u00fd thuy\u1ebft \u0111\u1ebfn th\u1ef1c h\u00e0nh\u201d<\/em> mang \u0111\u1ebfn cho b\u1ea1n \u0111\u1ecdc m\u1ed9t h\u00e0nh tr\u00ecnh tr\u1ecdn v\u1eb9n t\u1eeb n\u1ec1n t\u1ea3ng l\u00fd thuy\u1ebft, l\u1ecbch s\u1eed h\u00ecnh th\u00e0nh k\u1ef9 thu\u1eadt PCR cho \u0111\u1ebfn quy tr\u00ecnh th\u1ef1c h\u00e0nh chi ti\u1ebft v\u00e0 \u1ee9ng d\u1ee5ng th\u1ef1c ti\u1ec5n trong ph\u00f2ng th\u00ed nghi\u1ec7m.<\/p>\n\n\n\n

1.<\/strong> T\u1ed5ng quan v\u1ec1 quy\u1ec3n s\u00e1ch \u0111\u1ea7u tay<\/strong><\/h2>\n\n\n\n

\u201cK\u1ef9 thu\u1eadt PCR v\u00e0 Real-time PCR: T\u1eeb l\u00fd thuy\u1ebft \u0111\u1ebfn th\u1ef1c h\u00e0nh\u201d ch\u00ednh l\u00e0 quy\u1ec3n s\u00e1ch c\u00f4 \u0111\u1ecdng t\u1ea5t c\u1ea3 c\u00e1c ki\u1ebfn th\u1ee9c v\u00e0 kinh nghi\u1ec7m th\u1ef1c ti\u1ec5n c\u1ee7a c\u00e1c t\u00e1c gi\u1ea3 trong l\u0129nh v\u1ef1c sinh h\u1ecdc ph\u00e2n t\u1eed. M\u1edf \u0111\u1ea7u b\u1eb1ng b\u1ed1i c\u1ea3nh ra \u0111\u1eddi v\u00e0 l\u1ecbch s\u1eed ph\u00e1t tri\u1ec3n c\u1ee7a k\u1ef9 thu\u1eadt PCR c\u00f9ng v\u1edbi c\u00e1c s\u1ef1 ki\u1ec7n li\u00ean quan kh\u00e1c nhau, t\u1ea5t c\u1ea3 \u0111\u01b0\u1ee3c t\u00f3m t\u1eaft theo tr\u1ee5c th\u1eddi gian theo H\u00ecnh 1 d\u01b0\u1edbi \u0111\u00e2y.<\/p>\n\n\n\n

\"\"
H\u00ecnh 1: C\u00e1c c\u1ed9t m\u1ed1c quan tr\u1ecdng trong l\u1ecbch s\u1eed t\u00ecm ki\u1ebfm ngu\u1ed3n g\u1ed1c v\u00e0 ph\u00e1t tri\u1ec3n c\u1ee7a DNA<\/figcaption><\/figure>\n\n\n\n

C\u00f9ng v\u1edbi s\u1ef1 ph\u00e1t tri\u1ec3n c\u1ee7a k\u1ef9 thu\u1eadt khu\u1ebfch \u0111\u1ea1i ph\u00e2n t\u1eed, \u0111i\u1ec7n di l\u00e0 ph\u01b0\u01a1ng ph\u00e1p ph\u1ed5 bi\u1ebfn \u0111\u1ec3 \u0111\u1ecdc k\u1ebft qu\u1ea3 m\u00e0 k\u1ef9 thu\u1eadt PCR \u0111\u01b0a ra, gi\u00fap ph\u00e2n t\u00e1ch v\u00e0 ph\u00e1t hi\u1ec7n c\u00e1c ph\u00e2n t\u1eed sinh h\u1ecdc v\u1edbi c\u00e1c k\u00edch th\u01b0\u1edbc kh\u00e1c nhau nh\u01b0 nucleic acid v\u00e0 protein. Tuy nhi\u00ean, c\u00f3 nhi\u1ec1u y\u1ebfu t\u1ed1 \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn qu\u00e1 tr\u00ecnh \u0111i\u1ec7n di gel agarose nh\u01b0 t\u00ednh ch\u1ea5t c\u00f9a m\u1eabu, gel agarose, n\u1ed3ng \u0111\u1ed9 gel, \u0111\u1ed9 d\u00e0y gel, k\u00edch th\u01b0\u1edbc gi\u1ebfng,\u2026. Nh\u1eefng v\u1ea5n \u0111\u1ec1 th\u01b0\u1eddng g\u1eb7p trong qu\u00e1 tr\u00ecnh \u0111i\u1ec7n di v\u00e0 c\u00e1ch d\u1ef1 \u0111o\u00e1n nguy\u00ean nh\u00e2n \u0111\u00e3 \u0111\u01b0\u1ee3c c\u00e1c t\u00e1c gi\u1ea3 li\u1ec7t k\u00ea chi ti\u1ebft \u0111i k\u00e8m v\u1edbi c\u00e1c thi\u1ebft b\u1ecb th\u1ef1c hi\u1ec7n x\u00e9t nghi\u1ec7m.<\/p>\n\n\n\n

Theo th\u1eddi gian, ng\u00e0y c\u00e0ng c\u00f3 nhi\u1ec1u lo\u1ea1i k\u1ef9 thu\u1eadt PCR ph\u1ed5 bi\u1ebfn kh\u00e1c ra \u0111\u1eddi nh\u01b0 ph\u1ea3n \u1ee9ng chu\u1ed7i polymerase phi\u00ean m\u00e3 ng\u01b0\u1ee3c – reverse transcription polymerase chain reaction (RT-PCR), PCR l\u1ed3ng (nested PCR), PCR \u0111a t\u00e1c nh\u00e2n (Multiplex PCR). Trong s\u1ed1 \u0111\u00f3, Real-time PCR l\u00e0 m\u1ed9t c\u1ea3i ti\u1ebfn quan tr\u1ecdng c\u1ee7a PCR c\u01a1 b\u1ea3n, k\u1ef9 thu\u1eadt n\u00e0y kh\u00e1c bi\u1ec7t \u1edf vi\u1ec7c s\u1eed d\u1ee5ng thu\u1ed1c nhu\u1ed9m hu\u1ef3nh quang \u0111\u1ec3 t\u1ea1o ra c\u00e1c t\u00edn hi\u1ec7u khi nh\u00e2n b\u1ea3n DNA m\u1ee5c ti\u00eau v\u00e0 ph\u1ea3n \u00e1nh k\u1ebft qu\u1ea3 ph\u1ea3n \u1ee9ng theo th\u1eddi gian th\u1ef1c b\u1eb1ng thi\u1ebft b\u1ecb chuy\u00ean d\u1ee5ng.<\/p>\n\n\n\n

\"Nguy\u00ean
H\u00ecnh 2. Nguy\u00ean t\u1eafc c\u1ee7a k\u1ef9 thu\u1eadt Real-time PCR<\/figcaption><\/figure>\n\n\n\n

Thi\u1ebft b\u1ecb d\u00f9ng \u0111\u1ec3 th\u1ef1c hi\u1ec7n Real-time PCR kh\u00e1c v\u1edbi PCR \u1edf b\u1ed9 ph\u1eadn quang h\u1ecdc c\u00f3 ch\u1ee9c n\u0103ng \u0111\u1ecdc t\u00edn hi\u1ec7u hu\u1ef3nh quang \u0111\u01b0\u1ee3c ph\u00e1t ra t\u1eeb ph\u1ea3n \u1ee9ng. Hi\u1ec7n nay, tr\u00ean th\u1ecb tr\u01b0\u1eddng c\u00f3 nhi\u1ec1u d\u00f2ng m\u00e1y kh\u00e1c nhau v\u00e0 vi\u1ec7c l\u1ef1a ch\u1ecdn d\u00f2ng m\u00e1y ph\u00f9 h\u1ee3p c\u0169ng \u0111\u01b0\u1ee3c tr\u00ecnh b\u00e0y chi ti\u1ebft \u1edf n\u1ed9i dung thi\u1ebft b\u1ecb th\u1ef1c hi\u1ec7n Real-time PCR, c\u00f9ng m\u1ed9t s\u1ed1 l\u01b0u \u00fd v\u1ec1 c\u00e0i \u0111\u1eb7t v\u00e0 th\u00f4ng s\u1ed1 \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o \u0111\u1ed9 nh\u1ea1y v\u00e0 ch\u00ednh x\u00e1c. <\/p>\n\n\n\n

Nh\u00ecn chung, k\u1ef9 thu\u1eadt PCR v\u00e0 Real-time PCR \u0111\u01b0\u1ee3c \u1ee9ng d\u1ee5ng r\u1ed9ng r\u00e3i trong c\u00e1c l\u0129nh v\u1ef1c ch\u1ea9n \u0111o\u00e1n v\u00e0 nghi\u00ean c\u1ee9u b\u1edfi t\u00ednh ch\u00ednh x\u00e1c v\u00e0 kh\u1ea3 n\u0103ng th\u1ef1c hi\u1ec7n t\u01b0\u01a1ng \u0111\u1ed1i \u0111\u01a1n gi\u1ea3n.<\/p>\n\n\n\n

B\u00ean c\u1ea1nh \u0111\u00f3, \u0111\u1ec3 gi\u00fap cho \u0111\u1ed9c gi\u1ea3 n\u1eafm \u0111\u01b0\u1ee3c ki\u1ebfn th\u1ee9c c\u01a1 b\u1ea3n v\u1ec1 m\u1ed9t ph\u00f2ng th\u00ed nghi\u1ec7m \u0111\u1ea1t chu\u1ea9n<\/a>, nh\u00f3m t\u00e1c gi\u1ea3 \u0111\u00e3 \u0111\u1eb7c bi\u1ec7t bi\u00ean so\u1ea1n ch\u01b0\u01a1ng thi\u1ebft l\u1eadp ph\u00f2ng x\u00e9t nghi\u1ec7m ch\u1ea9n \u0111o\u00e1n sinh h\u1ecdc ph\u00e2n t\u1eed ti\u00eau chu\u1ea9n nh\u1eb1m h\u01b0\u1edbng d\u1eabn c\u00e1ch set up ph\u00f2ng th\u00ed nghi\u1ec7m v\u00e0 ki\u1ec3m so\u00e1t k\u1ebft qu\u1ea3 x\u00e9t nghi\u1ec7m sao cho \u0111\u1ea3m b\u1ea3o ch\u1ea5t l\u01b0\u1ee3ng nh\u1ea5t nh\u01b0 n\u1ed9i ki\u1ec3m, ngo\u1ea1i ki\u1ec3m v\u00e0 c\u00e1c bi\u1ec7n ph\u00e1p kh\u00e1c.<\/p>\n\n\n\n

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H\u00ecnh 3. S\u01a1 \u0111\u1ed3 thi\u1ebft k\u1ebf ph\u00f2ng th\u00ed nghi\u1ec7m Sinh h\u1ecdc ph\u00e2n t\u1eed theo h\u01b0\u1edbng d\u1eabn c\u1ee7a WHO<\/figcaption><\/figure>\n\n\n\n

Kh\u00f4ng ch\u1ec9 d\u1eebng l\u1ea1i \u1edf nh\u1eefng trang l\u00fd thuy\u1ebft, cu\u1ed1n s\u00e1ch c\u00f2n \u0111\u01b0\u1ee3c x\u00e2y d\u1ef1ng nh\u01b0 m\u1ed9t \u201cprotocol\u201d trong ph\u00f2ng th\u00ed nghi\u1ec7m. N\u1ed9i dung th\u1ef1c h\u00e0nh \u0111i t\u1eeb h\u01b0\u1edbng d\u1eabn thi\u1ebft k\u1ebf m\u1ed3i chuy\u00ean bi\u1ec7t \u0111\u1ebfn t\u00e1ch chi\u1ebft m\u1eabu, th\u1ef1c h\u00e0nh c\u00e1c k\u1ef9 thu\u1eadt PCR, k\u1ef9 thu\u1eadt Real-time PCR \u0111\u1ecbnh t\u00ednh, \u0111\u1ecbnh l\u01b0\u1ee3ng v\u00e0 phi\u00ean m\u00e3 ng\u01b0\u1ee3c. <\/p>\n\n\n\n

M\u1ed7i ch\u01b0\u01a1ng th\u1ef1c h\u00e0nh c\u00f3 m\u1ee5c ti\u00eau r\u00f5 r\u00e0ng, v\u1eadt li\u1ec7u ho\u00e1 ch\u1ea5t d\u1ee5ng c\u1ee5 c\u1ea7n chu\u1ea9n b\u1ecb v\u00e0 quy tr\u00ecnh chi ti\u1ebft \u0111\u1ebfn t\u1eebng b\u01b0\u1edbc. B\u1ea1n \u0111\u1ecdc c\u00f3 th\u1ec3 \u00e1p d\u1ee5ng quy tr\u00ecnh c\u00e1c b\u01b0\u1edbc, thay \u0111\u1ed5i v\u00e0 \u0111i\u1ec1u ch\u1ec9nh cho ph\u00f9 h\u1ee3p \u0111\u1ec3 th\u1ef1c h\u00e0nh trong ph\u00f2ng th\u00ed nghi\u1ec7m c\u1ee7a m\u00ecnh.<\/p>\n\n\n\n

2.<\/strong> \u0110\u1ed1i t\u01b0\u1ee3ng c\u1ee7a quy\u1ec3n s\u00e1ch<\/strong><\/h2>\n\n\n\n

S\u1ef1 kh\u00e1c bi\u1ec7t l\u00e0m n\u00ean gi\u00e1 tr\u1ecb c\u1ee7a cu\u1ed1n s\u00e1ch ch\u00ednh l\u00e0 ki\u1ebfn th\u1ee9c \u0111\u01b0\u1ee3c c\u00f4 \u0111\u1ecdng v\u00e0 bi\u00ean so\u1ea1n b\u1edfi nh\u1eefng ng\u01b0\u1eddi tr\u1ef1c ti\u1ebfp l\u00e0m nghi\u00ean c\u1ee9u m\u1ed7i ng\u00e0y, l\u00e0 quy\u1ec3n s\u00e1ch hay t\u1eeb ch\u00ednh tay “th\u1ee3 test” chuy\u00ean nghi\u1ec7p vi\u1ebft n\u00ean. M\u1ed7i ch\u01b0\u01a1ng kh\u00f4ng ch\u1ec9 tr\u00ecnh b\u00e0y quy tr\u00ecnh chu\u1ea9n, m\u00e0 c\u00f2n k\u00e8m theo nhi\u1ec1u case study th\u1ef1c t\u1ebf v\u00e0 m\u1eb9o x\u1eed l\u00fd l\u1ed7i khi l\u00e0m th\u00ed nghi\u1ec7m \u2013 nh\u1eefng kinh nghi\u1ec7m m\u00e0 hi\u1ebfm cu\u1ed1n s\u00e1ch tham kh\u1ea3o n\u00e0o \u0111\u1ec1 c\u1eadp chi ti\u1ebft \u0111\u1ebfn t\u1eebng nguy\u00ean nh\u00e2n v\u00e0 t\u00ecnh hu\u1ed1ng.<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u0110\u00e2y kh\u00f4ng ch\u1ec9 l\u00e0 m\u1ed9t t\u00e0i li\u1ec7u h\u1ecdc thu\u1eadt, quy\u1ec3n s\u00e1ch c\u00f2n \u0111\u00f3ng vai tr\u00f2 nh\u01b0 c\u1ea7u n\u1ed1i gi\u1eefa l\u00fd thuy\u1ebft v\u00e0 th\u1ef1c ti\u1ec5n ph\u00f2ng th\u00ed nghi\u1ec7m, tr\u1edf th\u00e0nh tr\u1ee3 th\u1ee7 \u0111\u1eafc l\u1ef1c cho k\u1ef9 thu\u1eadt vi\u00ean ph\u00f2ng x\u00e9t nghi\u1ec7m v\u00e0 c\u00e1n b\u1ed9 nghi\u00ean c\u1ee9u \u0111ang t\u00ecm hi\u1ec3u v\u1ec1 l\u0129nh v\u1ef1c sinh h\u1ecdc ph\u00e2n t\u1eed, c\u0169ng nh\u01b0 sinh vi\u00ean chuy\u00ean ng\u00e0nh sinh h\u1ecdc, c\u00f4ng ngh\u1ec7 sinh h\u1ecdc v\u00e0 c\u00e1c ng\u00e0nh x\u00e9t nghi\u1ec7m.<\/p>\n\n\n\n

Th\u00f4ng qua quy\u1ec3n s\u00e1ch, nh\u00f3m t\u00e1c gi\u1ea3 mong mu\u1ed1n chia s\u1ebb nh\u1eefng kinh nghi\u1ec7m \u201cth\u1ef1c chi\u1ebfn\u201d \u0111\u01b0\u1ee3c \u0111\u00fac k\u1ebft t\u1eeb nhi\u1ec1u n\u0103m g\u1eafn b\u00f3 v\u1edbi ph\u00f2ng th\u00ed nghi\u1ec7m, kh\u00f4ng ch\u1ec9 gi\u00fap ng\u01b0\u1eddi \u0111\u1ecdc hi\u1ec3u r\u00f5 nguy\u00ean l\u00fd, m\u00e0 c\u00f2n c\u00f3 th\u1ec3 l\u00e0m ch\u1ee7 k\u1ef9 thu\u1eadt PCR v\u00e0 Real-time PCR, \u1ee9ng d\u1ee5ng hi\u1ec7u qu\u1ea3 v\u00e0o nghi\u00ean c\u1ee9u, gi\u1ea3ng d\u1ea1y v\u00e0 th\u1ef1c ti\u1ec5n ch\u1ea9n \u0111o\u00e1n.<\/p>\n\n\n\n

3. Chia s\u1ebb v\u1ec1 h\u00e0nh tr\u00ecnh vi\u1ebft s\u00e1ch<\/strong><\/h2>\n\n\n\n

H\u00e0nh tr\u00ecnh bi\u00ean so\u1ea1n cu\u1ed1n s\u00e1ch b\u1eaft \u0111\u1ea7u t\u1eeb n\u0103m 2022, khi \u00fd t\u01b0\u1edfng \u0111\u01b0\u1ee3c h\u00ecnh th\u00e0nh v\u00e0 b\u1ea3n \u0111\u1ec1 c\u01b0\u01a1ng \u0111\u1ea7u ti\u00ean ra \u0111\u1eddi. Trong su\u1ed1t hai n\u0103m ti\u1ebfp theo, nh\u00f3m t\u00e1c gi\u1ea3 v\u1eeba \u0111\u1ea3m nhi\u1ec7m c\u00f4ng t\u00e1c chuy\u00ean m\u00f4n, v\u1eeba ki\u00ean tr\u00ec thu th\u1eadp t\u00e0i li\u1ec7u, nghi\u00ean c\u1ee9u v\u00e0 vi\u1ebft n\u00ean nh\u1eefng b\u1ea3n th\u1ea3o ban \u0111\u1ea7u.<\/p>\n\n\n\n

\u0110\u1ebfn cu\u1ed1i n\u0103m 2024, b\u1ea3n th\u1ea3o \u0111\u01b0\u1ee3c ho\u00e0n thi\u1ec7n v\u00e0 ch\u00ednh th\u1ee9c th\u00e0nh l\u1eadp h\u1ed9i \u0111\u1ed3ng nghi\u1ec7m thu v\u00e0o ng\u00e0y 09\/12\/2024. T\u1eeb nh\u1eefng g\u00f3p \u00fd s\u00e2u s\u1eafc c\u1ee7a qu\u00fd th\u1ea7y c\u00f4 trong h\u1ed9i \u0111\u1ed3ng, nh\u00f3m t\u00e1c gi\u1ea3 l\u1ea1i ti\u1ebfp t\u1ee5c h\u00e0nh tr\u00ecnh \u201cch\u1ea1y n\u01b0\u1edbc r\u00fat\u201d: ch\u1ec9nh s\u1eeda c\u1ea5u tr\u00fac, b\u1ed5 sung n\u1ed9i dung, tinh ch\u1ec9nh t\u1eebng ch\u01b0\u01a1ng, v\u1ebd l\u1ea1i h\u00ecnh minh h\u1ecda \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o tu\u00e2n th\u1ee7 b\u1ea3n quy\u1ec1n. <\/p>\n\n\n\n

B\u01b0\u1edbc sang 2025, sau khi b\u1ea3n th\u1ea3o ho\u00e0n ch\u1ec9nh \u0111\u01b0\u1ee3c chuy\u1ec3n \u0111\u1ebfn NXB \u0110\u1ea1i h\u1ecdc Qu\u1ed1c gia TP.HCM \u2013 m\u1ed9t \u0111\u01a1n v\u1ecb uy t\u00edn trong l\u0129nh v\u1ef1c gi\u00e1o d\u1ee5c, cu\u1ed1n s\u00e1ch \u201cK\u1ef9 thu\u1eadt PCR v\u00e0 Real-time PCR: T\u1eeb l\u00fd thuy\u1ebft \u0111\u1ebfn th\u1ef1c h\u00e0nh\u201d ch\u00ednh th\u1ee9c ra m\u1eaft b\u1ea1n \u0111\u1ecdc. <\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Nh\u00ecn l\u1ea1i ch\u1eb7ng \u0111\u01b0\u1eddng \u1ea5y, nh\u00f3m t\u00e1c gi\u1ea3 v\u1eabn nh\u1edb m\u00e3i nh\u1eefng k\u1ef7 ni\u1ec7m kh\u00f3 qu\u00ean: nh\u1eefng \u0111\u00eam th\u1ee9c tr\u1eafng mi\u1ec7t m\u00e0i b\u00ean trang b\u1ea3n th\u1ea3o, nh\u1eefng l\u1ea7n c\u0103ng th\u1eb3ng h\u1ecdp ch\u1ec9nh s\u1eeda hay nh\u1eefng l\u00fac x\u1eed l\u00fd h\u00ecnh \u1ea3nh tr\u00e1nh b\u1ea3n quy\u1ec1n. T\u1ea5t c\u1ea3 \u0111\u00e3 g\u00f3p ph\u1ea7n t\u1ea1o n\u00ean m\u1ed9t t\u00e1c ph\u1ea9m ghi d\u1ea5u l\u1ea1i th\u00e0nh t\u1ef1u nghi\u00ean c\u1ee9u c\u00f9ng s\u1ef1 ki\u00ean tr\u00ec v\u00e0 t\u00e2m huy\u1ebft c\u1ee7a nh\u00f3m t\u00e1c gi\u1ea3.<\/p>\n\n\n\n

4. T\u1ed5ng k\u1ebft<\/strong><\/h2>\n\n\n\n

Ch\u00fang t\u00f4i tr\u00e2n tr\u1ecdng m\u1eddi qu\u00fd \u0111\u1ed3ng nghi\u1ec7p, b\u1ea1n \u0111\u1ecdc quan t\u00e2m \u0111\u1ebfn l\u0129nh v\u1ef1c sinh h\u1ecdc ph\u00e2n t\u1eed \u0111\u00f3n \u0111\u1ecdc cu\u1ed1n s\u00e1ch \u201cK\u1ef9 thu\u1eadt PCR v\u00e0 Real-time PCR: T\u1eeb l\u00fd thuy\u1ebft \u0111\u1ebfn th\u1ef1c h\u00e0nh\u201d, chia s\u1ebb tr\u1ea3i nghi\u1ec7m v\u00e0 \u0111\u00f3ng g\u00f3p \u00fd ki\u1ebfn, m\u1ed7i ph\u1ea3n h\u1ed3i \u0111\u1ec1u l\u00e0 ngu\u1ed3n \u0111\u1ed9ng l\u1ef1c qu\u00fd gi\u00e1 gi\u00fap ch\u00fang t\u00f4i ti\u1ebfp t\u1ee5c ho\u00e0n thi\u1ec7n n\u1ed9i dung trong nh\u1eefng l\u1ea7n t\u00e1i b\u1ea3n sau. B\u00ean c\u1ea1nh \u0111\u00f3, ch\u00fang t\u00f4i mong mu\u1ed1n cu\u1ed1n s\u00e1ch s\u1ebd tr\u1edf th\u00e0nh c\u1ea7u n\u1ed1i g\u1eafn k\u1ebft c\u1ed9ng \u0111\u1ed3ng nghi\u00ean c\u1ee9u v\u00e0 th\u1ef1c h\u00e0nh sinh h\u1ecdc ph\u00e2n t\u1eed c\u00f9ng nhau chinh ph\u1ee5c th\u1ebf gi\u1edbi ph\u00e2n t\u1eed trong c\u00f4ng ngh\u1ec7 sinh h\u1ecdc.<\/p>\n\n\n\n

B\u1ea1n \u0111\u1ecdc c\u00f3 th\u1ec3 c\u1eadp nh\u1eadt ki\u1ebfn th\u1ee9c v\u00e0 s\u1edf h\u1eefu cu\u1ed1n s\u00e1ch qua website: abtvn.com ho\u1eb7c fanpage: https:\/\/www.facebook.com\/abtcorporation<\/a><\/p>\n\n\n\n

Khi s\u1eed d\u1ee5ng s\u00e1ch l\u00e0m t\u00e0i li\u1ec7u tham kh\u1ea3o, xin vui l\u00f2ng tr\u00edch d\u1eabn ngu\u1ed3n t\u00e0i li\u1ec7u tham kh\u1ea3o nh\u01b0 sau:<\/p>\n\n\n\n

Nguy\u1ec5n Th\u1ecb Thu Th\u1ea3o, \u0110\u1ed7 Ng\u1ecdc Di\u1ec5m Tr\u00fac, Nguy\u1ec5n Duy Kh\u00e1nh, & Phan Th\u1ecb Ng\u1ecdc Di\u1ec5m. (2025). K\u1ef9 thu\u1eadt PCR v\u00e0 Real-time PCR T\u1eeb l\u00fd thuy\u1ebft \u0111\u1ebfn th\u1ef1c h\u00e0nh<\/em>. Nh\u00e0 xu\u1ea5t b\u1ea3n \u0110\u1ea1i h\u1ecdc Qu\u1ed1c gia Th\u00e0nh ph\u1ed1 H\u1ed3 Ch\u00ed Minh.<\/p>\n\n\n\n

Nh\u00f3m t\u00e1c gi\u1ea3 c\u00f9ng t\u1eadp th\u1ec3 ABT xin ch\u00e2n th\u00e0nh c\u1ea3m \u01a1n s\u1ef1 quan t\u00e2m v\u00e0 y\u00eau th\u01b0\u01a1ng \u0111\u1ebfn t\u1eeb c\u00e1c \u0111\u1ed9c gi\u1ea3!<\/p>\n\n\n\n

<\/p>","protected":false},"excerpt":{"rendered":"

Trong b\u1ed1i c\u1ea3nh c\u00e1c k\u1ef9 thu\u1eadt sinh h\u1ecdc ph\u00e2n t\u1eed ng\u00e0y c\u00e0ng ph\u00e1t tri\u1ec3n, PCR v\u00e0 Real-time PCR \u0111ang tr\u1edf th\u00e0nh c\u00f4ng c\u1ee5 kh\u00f4ng th\u1ec3 thi\u1ebfu trong nghi\u00ean c\u1ee9u v\u00e0 ch\u1ea9n \u0111o\u00e1n. Cu\u1ed1n s\u00e1ch\u201cK\u1ef9 thu\u1eadt PCR v\u00e0 Real-time PCR: T\u1eeb l\u00fd thuy\u1ebft \u0111\u1ebfn th\u1ef1c h\u00e0nh\u201d mang \u0111\u1ebfn cho b\u1ea1n \u0111\u1ecdc m\u1ed9t h\u00e0nh tr\u00ecnh tr\u1ecdn v\u1eb9n 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Cu\u1ed1n s\u00e1ch\u201cK\u1ef9 thu\u1eadt PCR v\u00e0 Real-time PCR: T\u1eeb l\u00fd thuy\u1ebft \u0111\u1ebfn th\u1ef1c h\u00e0nh\u201d mang \u0111\u1ebfn cho b\u1ea1n \u0111\u1ecdc m\u1ed9t h\u00e0nh tr\u00ecnh tr\u1ecdn v\u1eb9n…","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/13435","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=13435"}],"version-history":[{"count":5,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/13435\/revisions"}],"predecessor-version":[{"id":13769,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/13435\/revisions\/13769"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/13759"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=13435"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/categories?post=13435"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/tags?post=13435"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}