{"id":13422,"date":"2025-09-27T08:55:00","date_gmt":"2025-09-27T01:25:00","guid":{"rendered":"https:\/\/abtvn.com\/?p=13422"},"modified":"2025-12-29T13:38:09","modified_gmt":"2025-12-29T06:08:09","slug":"huong-dan-doc-ket-qua-real-time-pcr-asfv","status":"publish","type":"post","link":"https:\/\/abtvn.com\/en\/huong-dan-doc-ket-qua-real-time-pcr-asfv\/","title":{"rendered":"<strong>H\u01b0\u1edbng d\u1eabn \u0111\u1ecdc k\u1ebft qu\u1ea3 Real-time PCR tr\u00ean D\u1ecbch t\u1ea3 l\u1ee3n ch\u00e2u Phi (ASFV)<\/strong>"},"content":{"rendered":"<p class=\"wp-block-paragraph\"><strong>D\u1ecbch t\u1ea3 l\u1ee3n Ch\u00e2u Phi (ASFV)<\/strong> l\u00e0 m\u1ed9t b\u1ec7nh do virus c\u00f3 kh\u1ea3 n\u0103ng l\u00e2y nhi\u1ec5m r\u1ea5t cao, g\u00e2y ra t\u1ef7 l\u1ec7 t\u1eed vong g\u1ea7n nh\u01b0 100% tr\u00ean l\u1ee3n nh\u00e0 v\u00e0 \u0111ang l\u00e0 m\u1ed1i \u0111e d\u1ecda kinh t\u1ebf nghi\u00eam tr\u1ecdng cho ng\u00e0nh ch\u0103n nu\u00f4i to\u00e0n c\u1ea7u. Virus ASF c\u00f3 24 ki\u1ec3u gen \u0111\u00e3 \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh, trong \u0111\u00f3 ki\u1ec3u gen II c\u00f3 \u0111\u1ed9c l\u1ef1c cao \u0111ang l\u01b0u h\u00e0nh r\u1ed9ng r\u00e3i t\u1ea1i ch\u00e2u \u00c2u v\u00e0 ch\u00e2u \u00c1, bao g\u1ed3m c\u1ea3 \u0110\u00f4ng Nam \u00c1. Vi\u1ec7c ph\u00e1t tri\u1ec3n m\u1ed9t c\u00f4ng c\u1ee5 ch\u1ea9n \u0111o\u00e1n ph\u00e2n t\u1eed nhanh v\u00e0 \u0111\u00e1ng tin c\u1eady l\u00e0 c\u1ef1c k\u1ef3 quan tr\u1ecdng \u0111\u1ec3 c\u00f3 th\u1ec3 \u00e1p d\u1ee5ng c\u00e1c bi\u1ec7n ph\u00e1p ki\u1ec3m so\u00e1t k\u1ecbp th\u1eddi. <\/p>\n\n\n\n<p class=\"wp-block-paragraph\">C\u00e1c ph\u01b0\u01a1ng ph\u00e1p truy\u1ec1n th\u1ed1ng nh\u01b0 ph\u00e2n l\u1eadp virus r\u1ea5t t\u1ed1n th\u1eddi gian, trong khi PCR truy\u1ec1n th\u1ed1ng l\u1ea1i c\u00f3 \u0111\u1ed9 nh\u1ea1y th\u1ea5p v\u00e0 \u0111\u00f2i h\u1ecfi c\u00e1c b\u01b0\u1edbc x\u1eed l\u00fd sau ph\u1ea3n \u1ee9ng. C\u00e1c c\u00f4ng ngh\u1ec7 m\u1edbi h\u01a1n nh\u01b0 LAMP hay RPA tuy c\u00f3 ti\u1ec1m n\u0103ng nh\u01b0ng thi\u1ebft k\u1ebf m\u1ed3i ph\u1ee9c t\u1ea1p v\u00e0 \u0111\u1ed9 nh\u1ea1y ch\u01b0a cao b\u1eb1ng PCR. Real-time PCR hi\u1ec7n l\u00e0 c\u00f4ng ngh\u1ec7 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng r\u1ed9ng r\u00e3i nh\u1ea5t nh\u1edd \u0111\u1ed9 nh\u1ea1y v\u00e0 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u v\u01b0\u1ee3t tr\u1ed9i.<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>T\u1ed1c \u0110\u1ed9 V\u01b0\u1ee3t Tr\u1ed9i: <\/strong>Real-time PCR c\u00f3 th\u1ec3 cung c\u1ea5p k\u1ebft qu\u1ea3 ch\u1ea9n \u0111o\u00e1n ch\u00ednh x\u00e1c trong v\u00f2ng ch\u01b0a \u0111\u1ea7y 4 gi\u1edd k\u1ec3 t\u1eeb khi m\u1eabu \u0111\u1ebfn ph\u00f2ng th\u00ed nghi\u1ec7m. T\u1ed1c \u0111\u1ed9 n\u00e0y cho ph\u00e9p c\u00e1c nh\u00e0 qu\u1ea3n l\u00fd trang tr\u1ea1i v\u00e0 c\u01a1 quan th\u00fa y \u0111\u01b0a ra c\u00e1c bi\u1ec7n ph\u00e1p can thi\u1ec7p ngay l\u1eadp t\u1ee9c\u2014c\u00e1ch ly, khoanh v\u00f9ng, kh\u1eed tr\u00f9ng\u2014nh\u1eb1m ng\u0103n ch\u1eb7n virus l\u00e2y lan r\u1ed9ng.<\/li>\n\n\n\n<li><strong>\u0110\u1ed9 Nh\u1ea1y T\u1ed1i \u01afu: <\/strong>M\u1ed9t trong nh\u1eefng \u01b0u \u0111i\u1ec3m l\u1edbn nh\u1ea5t c\u1ee7a Real-time PCR l\u00e0 kh\u1ea3 n\u0103ng ph\u00e1t hi\u1ec7n m\u1ed9t l\u01b0\u1ee3ng r\u1ea5t nh\u1ecf v\u1eadt li\u1ec7u di truy\u1ec1n (DNA) c\u1ee7a virus. \u0110i\u1ec1u n\u00e0y c\u00f3 ngh\u0129a l\u00e0 c\u00f3 th\u1ec3 ph\u00e1t hi\u1ec7n l\u1ee3n nhi\u1ec5m b\u1ec7nh ngay c\u1ea3 trong giai \u0111o\u1ea1n \u0111\u1ea7u, th\u01b0\u1eddng l\u00e0 tr\u01b0\u1edbc khi ch\u00fang bi\u1ec3u hi\u1ec7n c\u00e1c tri\u1ec7u ch\u1ee9ng l\u00e2m s\u00e0ng r\u00f5 r\u00e0ng. Vi\u1ec7c ph\u00e1t hi\u1ec7n s\u1edbm n\u00e0y l\u00e0 y\u1ebfu t\u1ed1 then ch\u1ed1t \u0111\u1ec3 d\u1eadp t\u1eaft \u1ed5 d\u1ecbch.<\/li>\n\n\n\n<li><strong>&nbsp;\u0110\u1ed9 \u0110\u1eb7c Hi\u1ec7u Cao: <\/strong>X\u00e9t nghi\u1ec7m Real-time PCR s\u1eed d\u1ee5ng c\u00e1c \u0111o\u1ea1n m\u1ed3i (primer) v\u00e0 m\u1eabu d\u00f2 (probe) \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf \u0111\u1eb7c bi\u1ec7t \u0111\u1ec3 ch\u1ec9 b\u00e1m v\u00e0o DNA c\u1ee7a ASFV, c\u1ee5 th\u1ec3 l\u00e0 gene B646L (p72) c\u00f3 t\u00ednh b\u1ea3o t\u1ed3n cao. \u0110i\u1ec1u n\u00e0y gi\u00fap gi\u1ea3m thi\u1ec3u t\u1ed1i \u0111a nguy c\u01a1 d\u01b0\u01a1ng t\u00ednh gi\u1ea3 do nh\u1ea7m l\u1eabn v\u1edbi c\u00e1c virus kh\u00e1c, mang l\u1ea1i k\u1ebft qu\u1ea3 c\u00f3 \u0111\u1ed9 tin c\u1eady cao \u0111\u1ec3 b\u1ea1n \u0111\u01b0a ra nh\u1eefng quy\u1ebft \u0111\u1ecbnh quan tr\u1ecdng.<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"><em>Real-time PCR kh\u00f4ng ch\u1ec9 nhanh h\u01a1n, n\u00f3 c\u00f2n an to\u00e0n h\u01a1n v\u00e0 \u0111\u00e1ng tin c\u1eady h\u01a1n, tr\u1edf th\u00e0nh c\u00f4ng c\u1ee5 \u0111\u01b0\u1ee3c T\u1ed5 ch\u1ee9c Th\u00fa y Th\u1ebf gi\u1edbi (WOAH) v\u00e0 c\u00e1c c\u01a1 quan th\u00fa y h\u00e0ng \u0111\u1ea7u khuy\u1ebfn ngh\u1ecb cho vi\u1ec7c ch\u1ea9n \u0111o\u00e1n ASFV.<\/em><\/p>\n\n\n\n<h2 class=\"wp-block-heading\" style=\"font-size:30px\"><strong>1. Lo\u1ea1i m\u1eabu s\u1eed d\u1ee5ng cho x\u00e9t nghi\u1ec7m ASFV<\/strong><\/h2>\n\n\n\n<h3 class=\"wp-block-heading\" style=\"font-size:28px\"><strong>1.1. M\u1eabu m\u00e1u to\u00e0n ph\u1ea7n, huy\u1ebft thanh, c\u01a1 quan n\u1ed9i t\u1ea1ng<\/strong><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">M\u00e1u to\u00e0n ph\u1ea7n (kh\u00f4ng s\u1eed d\u1ee5ng \u1ed1ng ch\u1ee9a heparin v\u00ec s\u1ebd g\u00e2y \u1ee9c ch\u1ebf ph\u1ea3n \u1ee9ng, huy\u1ebft thanh (kh\u00f4ng ch\u1ee9a ch\u1ea5t ch\u1ed1ng \u0111\u00f4ng EDTA v\u00e0 heparin), l\u00e1ch, h\u1ea1ch b\u1ea1ch huy\u1ebft, v\u00e0 Amidan, th\u1eadn t\u1eeb l\u1ee3n&nbsp; l\u00e0 nh\u1eefng m\u1eabu \u0111\u01b0\u1ee3c l\u1ef1a ch\u1ecdn h\u00e0ng \u0111\u1ea7u do c\u00f3 t\u1ea3i l\u01b0\u1ee3ng virus cao.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" style=\"font-size:28px\"><strong>1.2. <strong>C\u00e1c lo\u1ea1i m\u1eabu kh\u00e1c cho gi\u00e1m s\u00e1t<\/strong><\/strong><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">D\u1ecbch mi\u1ec7ng thu th\u1eadp b\u1eb1ng d\u00e2y th\u1eebng l\u00e0 m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p \u00edt t\u1ed1n c\u00f4ng v\u00e0 hi\u1ec7u qu\u1ea3 v\u1ec1 chi ph\u00ed \u0111\u1ec3 theo d\u00f5i \u1edf c\u1ea5p \u0111\u1ed9 \u0111\u00e0n, c\u00f3 th\u1ec3 ph\u00e1t hi\u1ec7n DNA c\u1ee7a ASFV t\u1eeb 3-5 ng\u00e0y sau khi nhi\u1ec5m, th\u01b0\u1eddng l\u00e0 tr\u01b0\u1edbc khi c\u00f3 d\u1ea5u hi\u1ec7u l\u00e2m s\u00e0ng r\u00f5 r\u00e0ng. Tuy nhi\u00ean, n\u1ed3ng \u0111\u1ed9 virus trong d\u1ecbch mi\u1ec7ng th\u1ea5p h\u01a1n trong m\u00e1u, l\u00e0m t\u0103ng nguy c\u01a1 \u00e2m t\u00ednh gi\u1ea3. T\u0103m b\u00f4ng m\u00f4i tr\u01b0\u1eddng c\u00f3 th\u1ec3 ph\u00e1t hi\u1ec7n s\u1ef1 t\u1ed3n t\u1ea1i c\u1ee7a DNA virus nh\u01b0ng c\u00f3 th\u1ec3 kh\u00f4ng t\u01b0\u01a1ng quan v\u1edbi kh\u1ea3 n\u0103ng l\u00e2y nhi\u1ec5m.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Storage condition:<\/strong> M\u1eabu c\u1ea7n \u0111\u01b0\u1ee3c b\u1ea3o qu\u1ea3n l\u1ea1nh \u1edf nhi\u1ec7t \u0111\u1ed9 t\u1eeb 2\u00b0C \u0111\u1ebfn 8\u00b0C trong v\u00f2ng 7 ng\u00e0y, ho\u1eb7c \u0111\u00f4ng l\u1ea1nh \u1edf -20\u00b0C hay -80\u00b0C n\u1ebfu c\u1ea7n b\u1ea3o qu\u1ea3n l\u00e2u h\u01a1n.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" style=\"font-size:30px\"><strong>2. <strong>T\u00e1ch chi\u1ebft DNA\/RNA<\/strong><\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">T\u00f9y thu\u1ed9c v\u00e0o lo\u1ea1i m\u1eabu (v\u00ed d\u1ee5: m\u1eabu b\u1ec7nh ph\u1ea9m t\u1eeb l\u1ee3n nh\u01b0 m\u00e1u to\u00e0n ph\u1ea7n, l\u00e1ch, h\u1ea1ch b\u1ea1ch huy\u1ebft; ho\u1eb7c c\u00e1c m\u1eabu m\u00f4i tr\u01b0\u1eddng nh\u01b0 d\u1ecbch ph\u1ebft b\u1ec1 m\u1eb7t,..) v\u00e0 t\u00e1c nh\u00e2n g\u00e2y b\u1ec7nh l\u00e0 virus D\u1ecbch t\u1ea3 l\u1ee3n Ch\u00e2u Phi (ASFV) m\u00e0 c\u1ea7n l\u1ef1a ch\u1ecdn b\u1ed9 kit t\u00e1ch chi\u1ebft DNA cho ph\u00f9 h\u1ee3p. L\u01b0u \u00fd: Lu\u00f4n tu\u00e2n th\u1ee7 h\u01b0\u1edbng d\u1eabn s\u1eed d\u1ee5ng c\u1ee7a nh\u00e0 s\u1ea3n xu\u1ea5t b\u1ed9 kit.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" style=\"font-size:30px\"><strong>3. <strong>\u0110\u1ecdc v\u00e0 ph\u00e2n t\u00edch k\u1ebft qu\u1ea3 Real-time PCR<\/strong><\/strong><\/h2>\n\n\n\n<h3 class=\"wp-block-heading\" style=\"font-size:28px\"><strong>3.1. T\u1ea7m quan tr\u1ecdng c\u1ee7a c\u00e1c \u0111\u1ed1i ch\u1ee9ng trong Real-time PCR<\/strong><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u0110\u1ed1i ch\u1ee9ng \u00e2m t\u00e1ch chi\u1ebft (Extraction Negative Control): <\/strong>l\u00e0 m\u1eabu kh\u00f4ng ch\u1ee9a v\u1eadt ch\u1ea5t di truy\u1ec1n (DNA\/RNA) \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n to\u00e0n b\u1ed9 quy tr\u00ecnh t\u00e1ch chi\u1ebft t\u01b0\u01a1ng t\u1ef1 c\u00e1c m\u1eabu th\u1eadt nh\u1eb1m ki\u1ec3m tra qu\u00e1 tr\u00ecnh t\u00e1ch chi\u1ebft c\u00f3 b\u1ecb nhi\u1ec5m ch\u00e9o hay kh\u00f4ng.<\/li>\n\n\n\n<li><strong>\u0110\u1ed1i ch\u1ee9ng \u00e2m (Negative Control):<\/strong> l\u00e0 m\u1eabu kh\u00f4ng ch\u1ee9a tr\u00ecnh t\u1ef1 gene m\u1ee5c ti\u00eau trong h\u1ed7n h\u1ee3p ph\u1ea3n \u1ee9ng qPCR, \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u c\u1ee7a m\u1ed3i s\u1eed d\u1ee5ng v\u00e0 x\u00e1c nh\u1eadn k\u1ebft qu\u1ea3 ch\u1ec9 khu\u1ebfch \u0111\u1ea1i tr\u00ecnh t\u1ef1 gene m\u1ee5c ti\u00eau. N\u01b0\u1edbc si\u00eau s\u1ea1ch kh\u00f4ng ch\u1ee9a DNAse v\u00e0 RNAse (nuclease-free water) \u0111\u01b0\u1ee3c th\u00eam v\u00e0o h\u1ed7n h\u1ee3p master mix nh\u1eb1m ki\u1ec3m tra xem h\u00f3a ch\u1ea5t v\u00e0 thi\u1ebft b\u1ecb c\u00f3 b\u1ecb ngo\u1ea1i nhi\u1ec5m trong qu\u00e1 tr\u00ecnh n\u1ea1p m\u1eabu hay t\u1eeb s\u1ea3n ph\u1ea9m khu\u1ebfch \u0111\u1ea1i tr\u01b0\u1edbc \u0111\u00f3 hay kh\u00f4ng.<\/li>\n\n\n\n<li><strong>\u0110\u1ed1i ch\u1ee9ng d\u01b0\u01a1ng<\/strong> <strong>(Positive Control): <\/strong>l\u00e0 m\u1ed9t ch\u1ea5t chu\u1ea9n (Standard) ho\u1eb7c l\u00e0 m\u1eabu axit nucleic c\u00f3 s\u1ed1 l\u01b0\u1ee3ng b\u1ea3n sao \u0111\u00e3 bi\u1ebft tr\u01b0\u1edbc (Amplicon) gi\u1ed1ng v\u1edbi tr\u00ecnh t\u1ef1 c\u1ee7a DNA \u0111\u00edch. K\u1ebft qu\u1ea3 c\u1ee7a \u0111\u1ed1i ch\u1ee9ng d\u01b0\u01a1ng lu\u00f4n d\u01b0\u01a1ng t\u00ednh \u0111\u1ec3 ch\u1ee9ng minh s\u1ef1 ho\u1ea1t \u0111\u1ed9ng hi\u1ec7u qu\u1ea3 c\u1ee7a PCR mix \u0111ang s\u1eed d\u1ee5ng, \u0111\u1ea3m b\u1ea3o \u0111\u1ed9 nh\u1ea1y v\u00e0 \u0111\u1eb7c hi\u1ec7u c\u1ee7a c\u1eb7p m\u1ed3i, x\u00e1c nh\u1eadn nhi\u1ec7t \u0111\u1ed9 b\u1eaft c\u1eb7p l\u00e0 ch\u00ednh x\u00e1c, th\u1eddi gian k\u00e9o d\u00e0i \u0111\u1ee7 v\u00e0 kh\u00f4ng c\u00f3 ch\u1ea5t \u1ee9c ch\u1ebf PCR.<\/li>\n\n\n\n<li><strong>Ch\u1ee9ng n\u1ed9i (Internal Control \u2013 IC):<\/strong> l\u00e0 \u0111o\u1ea1n DNA\/RNA \u0111\u01b0\u1ee3c khu\u1ebfch \u0111\u1ea1i c\u00f9ng v\u1edbi tr\u00ecnh t\u1ef1 m\u1ee5c ti\u00eau trong c\u00f9ng m\u1ed9t ph\u1ea3n \u1ee9ng real-time PCR ho\u1eb7c trong c\u00f9ng m\u1ed9t l\u1ea7n ch\u1ea1y real-time PCR. Ch\u1ee9ng n\u1ed9i th\u01b0\u1eddng d\u00f9ng m\u1ed9t \u0111o\u1ea1n gene kh\u00f4ng c\u1ea1nh tranh v\u1edbi gene \u0111\u00edch, \u0111\u01b0\u1ee3c \u0111\u01b0a v\u00e0o qu\u00e1 tr\u00ecnh t\u00e1ch chi\u1ebft ho\u1eb7c pha mix, m\u1ee5c \u0111\u00edch \u0111\u1ec3 ki\u1ec3m qua ch\u1ea5t l\u01b0\u1ee3ng ph\u1ea3n \u1ee9ng PCR, ki\u1ec3m tra s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a ch\u1ea5t \u1ee9c ch\u1ebf c\u00f3 trong m\u1eabu.<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\" style=\"font-size:28px\"><strong>3.2. Ph\u00e2n t\u00edch k\u1ebft qu\u1ea3 Real-time PCR tr\u00ean ASFV<\/strong><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">Khi <strong><a href=\"https:\/\/abtvn.com\/en\/phan-tich-ket-qua-real-time-pcr\/\" data-type=\"link\" data-id=\"https:\/\/abtvn.com\/phan-tich-ket-qua-real-time-pcr\/\">ph\u00e2n t\u00edch k\u1ebft qu\u1ea3 real-time PCR<\/a><\/strong> c\u1ea7n \u0111\u1ecdc k\u1ebft h\u1ee3p d\u1eef li\u1ec7u th\u00f4 v\u00e0 d\u1eef li\u1ec7u ph\u00e2n t\u00edch l\u00e0 r\u1ea5t quan tr\u1ecdng \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o \u0111\u1ed9 tin c\u1eady v\u00e0 \u0111\u00e1nh gi\u00e1 ch\u00ednh x\u00e1c k\u1ebft qu\u1ea3, h\u1ea1n ch\u1ebf c\u00e1c tr\u01b0\u1eddng h\u1ee3p \u00e2m t\u00ednh gi\u1ea3, d\u01b0\u01a1ng t\u00ednh gi\u1ea3.<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>D\u1eef li\u1ec7u th\u00f4<\/strong> l\u00e0 d\u1eef li\u1ec7u g\u1ed1c thu \u0111\u01b0\u1ee3c tr\u1ef1c ti\u1ebfp t\u1eeb m\u00e1y real-time PCR trong qu\u00e1 tr\u00ecnh ch\u1ea1y ph\u1ea3n \u1ee9ng, tr\u01b0\u1edbc khi \u0111\u01b0\u1ee3c ph\u1ea7n m\u1ec1m x\u1eed l\u00fd t\u1ef1 \u0111\u1ed9ng.<\/li>\n\n\n\n<li><strong>D\u1eef li\u1ec7u ph\u00e2n t\u00edch<\/strong> l\u00e0 k\u1ebft qu\u1ea3 \u0111\u00e3 \u0111\u01b0\u1ee3c ph\u1ea7n m\u1ec1m x\u1eed l\u00fd t\u1eeb d\u1eef li\u1ec7u th\u00f4, cho ra c\u00e1c gi\u00e1 tr\u1ecb c\u1ee5 th\u1ec3 d\u00f9ng \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 \u0111\u1ecbnh t\u00ednh ho\u1eb7c \u0111\u1ecbnh l\u01b0\u1ee3ng.<\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full\"><img fetchpriority=\"high\" decoding=\"async\" width=\"975\" height=\"437\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2025\/10\/image-2.png\" alt=\"H\u00ecnh 1. K\u1ebft qu\u1ea3 Real-time PCR\" class=\"wp-image-13423\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2025\/10\/image-2.png 975w, https:\/\/abtvn.com\/wp-content\/uploads\/2025\/10\/image-2-300x134.png 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2025\/10\/image-2-768x344.png 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2025\/10\/image-2-18x8.png 18w\" sizes=\"(max-width: 975px) 100vw, 975px\" \/><figcaption class=\"wp-element-caption\"><em><strong>Figure 1. <\/strong>K\u1ebft qu\u1ea3 Real-time PCR tr\u00ean D\u1ecbch t\u1ea3 l\u1ee3n ch\u00e2u Phi (ASFV)<\/em><\/figcaption><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Step 1:<\/strong> Ki\u1ec3m tra h\u1ec7 th\u1ed1ng \u0111\u1ed1i ch\u1ee9ng<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Ch\u1ee9ng \u00e2m t\u00e1ch chi\u1ebft<\/strong> ph\u1ea3i cho k\u1ebft qu\u1ea3 \u00e2m t\u00ednh, \u0111\u1ea3m b\u1ea3o h\u00f3a ch\u1ea5t t\u00e1ch chi\u1ebft v\u00e0 qu\u00e1 tr\u00ecnh thao t\u00e1c t\u00e1ch chi\u1ebft kh\u00f4ng b\u1ecb ngo\u1ea1i nhi\u1ec5m.<\/li>\n\n\n\n<li><strong>Ch\u1ee9ng \u00e2m PCR<\/strong> ph\u1ea3i cho k\u1ebft qu\u1ea3 \u00e2m t\u00ednh, \u0111\u1ea3m b\u1ea3o \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u c\u1ee7a ph\u1ea3n \u1ee9ng, h\u00f3a ch\u1ea5t real-time PCR v\u00e0 thi\u1ebft b\u1ecb kh\u00f4ng b\u1ecb ngo\u1ea1i nhi\u1ec5m trong qu\u00e1 tr\u00ecnh n\u1ea1p m\u1eabu, hay t\u1eeb s\u1ea3n ph\u1ea9m khu\u1ebfch \u0111\u1ea1i tr\u01b0\u1edbc \u0111\u00f3.<\/li>\n\n\n\n<li><strong>Ch\u1ee9ng d\u01b0\u01a1ng<\/strong> ph\u1ea3i cho k\u1ebft qu\u1ea3 d\u01b0\u01a1ng t\u00ednh, \u0111\u1ea3m b\u1ea3o c\u00e1c th\u00e0nh ph\u1ea7n trong ph\u1ea3n \u1ee9ng real-time PCR v\u00e0 thi\u1ebft b\u1ecb ho\u1ea1t \u0111\u1ed9ng b\u00ecnh th\u01b0\u1eddng.<\/li>\n\n\n\n<li><strong>Ch\u1ee9ng n\u1ed9i IC<\/strong>:\n<ul class=\"wp-block-list\">\n<li>Th\u00eam v\u00e0o qu\u00e1 tr\u00ecnh t\u00e1ch chi\u1ebft: Ch\u1ee9ng \u00e2m t\u00e1ch chi\u1ebft v\u00e0 c\u00e1c m\u1eabu ph\u1ea3i cho k\u1ebft qu\u1ea3 d\u01b0\u01a1ng t\u00ednh v\u1edbi ch\u1ee9ng n\u1ed9i. T\u1eeb \u0111\u00f3 ki\u1ec3m tra qu\u00e1 tr\u00ecnh t\u00e1ch chi\u1ebft c\u00f3 th\u00e0nh c\u00f4ng hay kh\u00f4ng, m\u1eabu c\u00f3 ch\u1ea5t \u1ee9c ch\u1ebf ph\u1ea3n \u1ee9ng real-time PCR hay kh\u00f4ng.<\/li>\n\n\n\n<li>Th\u00eam v\u00e0o qu\u00e1 tr\u00ecnh n\u1ea1p m\u1eabu: Ch\u1ee9ng \u00e2m v\u00e0 c\u00e1c m\u1eabu ph\u1ea3i cho k\u1ebft qu\u1ea3 d\u01b0\u01a1ng t\u00ednh v\u1edbi ch\u1ee9ng n\u1ed9i. T\u1eeb \u0111\u00f3 ki\u1ec3m tra m\u1eabu c\u00f3 ch\u1ea5t \u1ee9c ch\u1ebf ph\u1ea3n \u1ee9ng real-time PCR hay kh\u00f4ng.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Step 2:<\/strong> Thi\u1ebft l\u1eadp baseline (\u0111\u01b0\u1eddng n\u1ec1n)<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Baseline:<\/strong> l\u00e0 \u0111\u01b0\u1eddng bi\u1ec3u di\u1ec5n m\u1ee9c \u0111\u1ed9 t\u00edn hi\u1ec7u trong su\u1ed1t nh\u1eefng chu k\u1ef3 \u0111\u1ea7u c\u1ee7a real-time PCR. Vi\u1ec7c thi\u1ebft l\u1eadp baseline l\u00e0 b\u01b0\u1edbc quan tr\u1ecdng trong ph\u00e2n t\u00edch k\u1ebft qu\u1ea3 real-time PCR do baseline kh\u00f4ng c\u1ed1 \u0111\u1ecbnh, gi\u00e1 tr\u1ecb baseline s\u1ebd \u1ea3nh h\u01b0\u1edfng nhi\u1ec1u \u0111\u1ebfn k\u1ebft qu\u1ea3 cu\u1ed1i c\u00f9ng. N\u1ebfu \u0111i\u1ec1u ch\u1ec9nh gi\u00e1 tr\u1ecb baseline qu\u00e1 th\u1ea5p, k\u1ebft qu\u1ea3 s\u1ebd r\u1ea5t d\u1ec5 thu nh\u1eadn t\u00edn hi\u1ec7u nhi\u1ec5u (d\u01b0\u01a1ng t\u00ednh gi\u1ea3), ng\u01b0\u1ee3c l\u1ea1i gi\u00e1 tr\u1ecb baseline qu\u00e1 cao, k\u1ebft qu\u1ea3 s\u1ebd c\u00f3 \u0111\u1ed9 ch\u00ednh x\u00e1c th\u1ea5p v\u00ec b\u1ecf qua c\u00e1c m\u1eabu d\u01b0\u01a1ng y\u1ebfu (\u00e2m t\u00ednh gi\u1ea3). V\u00ec v\u1eady, baseline n\u00ean \u0111\u01b0\u1ee3c \u0111i\u1ec1u ch\u1ec9nh v\u1ec1 kho\u1ea3ng 5% so v\u1edbi gi\u00e1 tr\u1ecb t\u00edn hi\u1ec7u hu\u1ef3nh quang ch\u1ee9ng d\u01b0\u01a1ng.<\/li>\n\n\n\n<li><strong>Gi\u00e1 tr\u1ecb ng\u01b0\u1ee1ng c\u1eaft (Cut-off):<\/strong> M\u1ed7i x\u00e9t nghi\u1ec7m \u0111\u00e3 \u0111\u01b0\u1ee3c x\u00e1c th\u1ef1c \u0111\u1ec1u c\u00f3 m\u1ed9t gi\u00e1 tr\u1ecb Ct Cut-off \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh tr\u01b0\u1edbc \u0111\u1ec3 ph\u00e2n bi\u1ec7t k\u1ebft qu\u1ea3 d\u01b0\u01a1ng t\u00ednh v\u00e0 \u00e2m t\u00ednh. Ng\u01b0\u1ee1ng n\u00e0y \u0111\u01b0\u1ee3c thi\u1ebft l\u1eadp d\u1ef1a tr\u00ean Gi\u1edbi h\u1ea1n ph\u00e1t hi\u1ec7n (LOD) c\u1ee7a b\u1ed9 kit.<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Step 3: <\/strong>Result analysis<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Sau khi \u0111i\u1ec1u ch\u1ec9nh baseline, ti\u1ebfn h\u00e0nh \u0111\u1ecdc k\u1ebft qu\u1ea3, ghi nh\u1eadn gi\u00e1 tr\u1ecb Ct v\u00e0 ph\u00e2n t\u00edch k\u1ebft qu\u1ea3 theo h\u01b0\u1edbng d\u1eabn s\u1eed d\u1ee5ng c\u1ee7a b\u1ed9 kit real-time \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng.<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full\"><img decoding=\"async\" width=\"975\" height=\"276\" data-src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2025\/10\/image-3.png\" alt=\"H\u00ecnh 2. Ph\u00e2n t\u00edch k\u1ebft qu\u1ea3 Real-time PCR tr\u00ean D\u1ecbch t\u1ea3 l\u1ee3n ch\u00e2u Phi (ASFV)\" class=\"wp-image-13424 lazyload\" data-srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2025\/10\/image-3.png 975w, https:\/\/abtvn.com\/wp-content\/uploads\/2025\/10\/image-3-300x85.png 300w, https:\/\/abtvn.com\/wp-content\/uploads\/2025\/10\/image-3-768x217.png 768w, https:\/\/abtvn.com\/wp-content\/uploads\/2025\/10\/image-3-18x5.png 18w\" data-sizes=\"(max-width: 975px) 100vw, 975px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 975px; --smush-placeholder-aspect-ratio: 975\/276;\" \/><figcaption class=\"wp-element-caption\"><em><strong>Figure 2. <\/strong>Analysis of Real-Time PCR Results for DNA RNA Detection<\/em> <em>tr\u00ean D\u1ecbch t\u1ea3 l\u1ee3n ch\u00e2u Phi (ASFV)<\/em><\/figcaption><\/figure>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>M\u1eabu \u00e2m t\u00ednh: <\/strong>l\u00e0 m\u1eabu kh\u00f4ng c\u00f3 \u0111\u01b0\u1eddng t\u00edn hi\u1ec7u khu\u1ebfch \u0111\u1ea1i v\u00e0 kh\u00f4ng c\u00f3 gi\u00e1 tr\u1ecb Ct \u1edf k\u00eanh t\u00e1c nh\u00e2n (No Ct: 0.00). \u0110\u1ed1i v\u1edbi m\u1eabu \u00e2m t\u00ednh, b\u1eaft bu\u1ed9c lu\u00f4n xu\u1ea5t hi\u1ec7n \u0111\u01b0\u1eddng t\u00edn hi\u1ec7u khu\u1ebfch \u0111\u1ea1i \u1edf k\u00eanh ch\u1ee9ng n\u1ed9i.<\/li>\n\n\n\n<li><strong>M\u1eabu D\u01b0\u01a1ng t\u00ednh: <\/strong>l\u00e0 m\u1eabu c\u00f3 \u0111\u01b0\u1eddng t\u00edn hi\u1ec7u khu\u1ebfch \u0111\u1ea1i v\u00e0 c\u00f3 gi\u00e1 tr\u1ecb Ct (gi\u00e1 tr\u1ecb Ct &lt; 35) \u1edf k\u00eanh t\u00e1c nh\u00e2n. \u0110\u1ed1i v\u1edbi m\u1eabu d\u01b0\u01a1ng t\u00ednh, xu\u1ea5t hi\u1ec7n \u0111\u01b0\u1eddng t\u00edn hi\u1ec7u khu\u1ebfch \u0111\u1ea1i c\u1ee7a c\u1ea3 k\u00eanh t\u00e1c nh\u00e2n v\u00e0 k\u00eanh ch\u1ee9ng n\u1ed9i. Trong m\u1ed9t s\u1ed1 tr\u01b0\u1eddng h\u1ee3p gi\u00e1 tr\u1ecb Ct c\u1ee7a k\u00eanh t\u00e1c nh\u00e2n l\u00ean s\u1edbm d\u1eabn \u0111\u1ebfn t\u00edn hi\u1ec7u k\u00eanh ch\u1ee9ng n\u1ed9i \u00e2m t\u00ednh.<\/li>\n\n\n\n<li><strong>Kh\u00f4ng th\u1ec3 k\u1ebft lu\u1eadn: <\/strong>\u0110\u01b0\u1eddng t\u00edn hi\u1ec7u \u1edf c\u1ea3 k\u00eanh t\u00e1c nh\u00e2n v\u00e0 k\u00eanh ch\u1ee9ng n\u1ed9i \u0111\u1ec1u \u00e2m t\u00ednh. Trong tr\u01b0\u1eddng h\u1ee3p n\u00e0y, c\u00f3 th\u1ec3 m\u1eabu \u0111ang t\u1ed3n t\u1ea1i ch\u1ea5t \u1ee9c ch\u1ebf, c\u1ea7n pha lo\u00e3ng m\u1eabu 10 l\u1ea7n \u0111\u1ec3 ti\u1ebfn h\u00e0nh x\u00e9t nghi\u1ec7m l\u1ea1i.<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">Trong ch\u1ea9n \u0111o\u00e1n b\u1ec7nh tr\u00ean l\u1ee3n, k\u1ebft qu\u1ea3 d\u01b0\u01a1ng t\u00ednh b\u1eb1ng Real-time PCR v\u1edbi ASFV c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c ph\u00e1t hi\u1ec7n ngay c\u1ea3 trong giai \u0111o\u1ea1n \u1ee7 b\u1ec7nh, tr\u01b0\u1edbc khi l\u1ee3n bi\u1ec3u hi\u1ec7n tri\u1ec7u ch\u1ee9ng l\u00e2m s\u00e0ng r\u00f5 r\u1ec7t. Nh\u1edd \u0111\u1ed9 nh\u1ea1y v\u00e0 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u v\u01b0\u1ee3t tr\u1ed9i, ph\u01b0\u01a1ng ph\u00e1p n\u00e0y cho ph\u00e9p x\u00e1c \u0111\u1ecbnh s\u1ef1 c\u00f3 m\u1eb7t c\u1ee7a m\u1ea7m b\u1ec7nh \u1edf n\u1ed3ng \u0111\u1ed9 th\u1ea5p, \u0111\u00f3ng vai tr\u00f2 nh\u01b0 m\u1ed9t h\u1ec7 th\u1ed1ng c\u1ea3nh b\u00e1o s\u1edbm c\u1ef1c k\u1ef3 gi\u00e1 tr\u1ecb.Vi\u1ec7c ph\u00e1t hi\u1ec7n s\u1edbm n\u00e0y l\u00e0 y\u1ebfu t\u1ed1 then ch\u1ed1t, cho ph\u00e9p c\u00e1c c\u01a1 quan ch\u1ee9c n\u0103ng v\u00e0 ng\u01b0\u1eddi ch\u0103n nu\u00f4i tri\u1ec3n khai ngay l\u1eadp t\u1ee9c c\u00e1c bi\u1ec7n ph\u00e1p an to\u00e0n sinh h\u1ecdc, khoanh v\u00f9ng, c\u00e1ch ly v\u00e0 x\u1eed l\u00fd d\u1ecbch b\u1ec7nh theo quy \u0111\u1ecbnh.Do \u0111\u00f3, k\u1ebft qu\u1ea3 ch\u1ea9n \u0111o\u00e1n b\u1eb1ng Real-time PCR kh\u00f4ng ch\u1ec9 \u0111\u01a1n thu\u1ea7n x\u00e1c nh\u1eadn t\u00ecnh tr\u1ea1ng b\u1ec7nh c\u1ee7a m\u1ed9t c\u00e1 th\u1ec3, m\u00e0 c\u00f2n l\u00e0 c\u01a1 s\u1edf khoa h\u1ecdc v\u1eefng ch\u1eafc v\u00e0 k\u1ecbp th\u1eddi nh\u1eb1m ng\u0103n ch\u1eb7n s\u1ef1 l\u00e2y lan c\u1ee7a D\u1ecbch t\u1ea3 l\u1ee3n Ch\u00e2u Phi, b\u1ea3o v\u1ec7 t\u1ed5ng \u0111\u00e0n v\u00e0 gi\u1ea3m thi\u1ec3u thi\u1ec7t h\u1ea1i kinh t\u1ebf \u1edf m\u1ee9c t\u1ed1i \u0111a.<\/p>\n\n\n\n<div style=\"height:26px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" style=\"font-size:30px\"><strong>T\u00e0i li\u1ec7u tham kh\u1ea3o<\/strong><\/h2>\n\n\n\n<ol class=\"wp-block-list\">\n<li>Hwang, H. J., Choi, Y. S., Song, K., Frant, M., &amp; Kim, J. H. (2023). <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/36686190\/\" data-type=\"link\" data-id=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/36686190\/\" target=\"_blank\" rel=\"noopener\">Development and validation of a fast quantitative real-time PCR assay for the detection of African swine fever virus.&nbsp;<\/a><em>Frontiers in Veterinary Science<\/em>,&nbsp;<em>9<\/em>, 1037728.<\/li>\n\n\n\n<li>Galindo, I., &amp; Alonso, C. (2017). African swine fever virus: a review.&nbsp;<em>Viruses<\/em>,&nbsp;<em>9<\/em>(5), 103.<\/li>\n\n\n\n<li>Penrith, M. L., Van Emmenes, J., Hakizimana, J. N., Heath, L., Kabuuka, T., Misinzo, G., &#8230; &amp; Luka, P. D. (2024). African swine fever diagnosis in Africa: challenges and opportunities.&nbsp;<em>Pathogens<\/em>,&nbsp;<em>13<\/em>(4), 296.<\/li>\n\n\n\n<li>Chi C\u1ee5c Th\u00fa Y V\u00f9ng VI, 2017.&nbsp;<em>Quy tr\u00ecnh ph\u00e1t hi\u1ec7n vi r\u00fat d\u1ecbch t\u1ea3 l\u1ee3n Ch\u00e2u Phi b\u1eb1ng k\u1ef9 thu\u1eadt realtime PCR.<\/em><\/li>\n\n\n\n<li>TCVN 8400-41:2019 v\u1ec1 B\u1ec7nh \u0111\u1ed9ng v\u1eadt &#8211; Quy tr\u00ecnh ch\u1ea9n \u0111o\u00e1n &#8211; Ph\u1ea7n 41: B\u1ec7nh D\u1ecbch t\u1ea3 l\u1ee3n ch\u00e2u Phi.<\/li>\n<\/ol>","protected":false},"excerpt":{"rendered":"<p>D\u1ecbch t\u1ea3 l\u1ee3n Ch\u00e2u Phi (ASFV) l\u00e0 m\u1ed9t b\u1ec7nh do virus c\u00f3 kh\u1ea3 n\u0103ng l\u00e2y nhi\u1ec5m r\u1ea5t cao, g\u00e2y ra t\u1ef7 l\u1ec7 t\u1eed vong g\u1ea7n nh\u01b0 100% tr\u00ean l\u1ee3n nh\u00e0 v\u00e0 \u0111ang l\u00e0 m\u1ed1i \u0111e d\u1ecda kinh t\u1ebf nghi\u00eam tr\u1ecdng cho ng\u00e0nh ch\u0103n nu\u00f4i to\u00e0n c\u1ea7u. Virus ASF c\u00f3 24 ki\u1ec3u gen \u0111\u00e3 \u0111\u01b0\u1ee3c x\u00e1c [&hellip;]<\/p>","protected":false},"author":1,"featured_media":13482,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[228,267],"tags":[476,448],"class_list":["post-13422","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-bai-viet","category-sinh-hoc-phan-tu-bai-viet","tag-real-time-pcr","tag-sinh-hoc-phan-tu"],"uagb_featured_image_src":{"full":["https:\/\/abtvn.com\/wp-content\/uploads\/2025\/09\/B-ASFV.png",1920,1080,false],"thumbnail":["https:\/\/abtvn.com\/wp-content\/uploads\/2025\/09\/B-ASFV-150x150.png",150,150,true],"medium":["https:\/\/abtvn.com\/wp-content\/uploads\/2025\/09\/B-ASFV-300x169.png",300,169,true],"medium_large":["https:\/\/abtvn.com\/wp-content\/uploads\/2025\/09\/B-ASFV-768x432.png",768,432,true],"large":["https:\/\/abtvn.com\/wp-content\/uploads\/2025\/09\/B-ASFV-1024x576.png",1024,576,true],"1536x1536":["https:\/\/abtvn.com\/wp-content\/uploads\/2025\/09\/B-ASFV-1536x864.png",1536,864,true],"2048x2048":["https:\/\/abtvn.com\/wp-content\/uploads\/2025\/09\/B-ASFV.png",1920,1080,false],"trp-custom-language-flag":["https:\/\/abtvn.com\/wp-content\/uploads\/2025\/09\/B-ASFV-18x10.png",18,10,true],"woocommerce_thumbnail":["https:\/\/abtvn.com\/wp-content\/uploads\/2025\/09\/B-ASFV-1707x960.png",1707,960,true],"woocommerce_single":["https:\/\/abtvn.com\/wp-content\/uploads\/2025\/09\/B-ASFV.png",1920,1080,false],"woocommerce_gallery_thumbnail":["https:\/\/abtvn.com\/wp-content\/uploads\/2025\/09\/B-ASFV-100x100.png",100,100,true]},"uagb_author_info":{"display_name":"ABT Team","author_link":"https:\/\/abtvn.com\/en\/author\/admin\/"},"uagb_comment_info":0,"uagb_excerpt":"D\u1ecbch t\u1ea3 l\u1ee3n Ch\u00e2u Phi (ASFV) l\u00e0 m\u1ed9t b\u1ec7nh do virus c\u00f3 kh\u1ea3 n\u0103ng l\u00e2y nhi\u1ec5m r\u1ea5t cao, g\u00e2y ra t\u1ef7 l\u1ec7 t\u1eed vong g\u1ea7n nh\u01b0 100% tr\u00ean l\u1ee3n nh\u00e0 v\u00e0 \u0111ang l\u00e0 m\u1ed1i \u0111e d\u1ecda kinh t\u1ebf nghi\u00eam tr\u1ecdng cho ng\u00e0nh ch\u0103n nu\u00f4i to\u00e0n c\u1ea7u. Virus ASF c\u00f3 24 ki\u1ec3u gen \u0111\u00e3 \u0111\u01b0\u1ee3c x\u00e1c&hellip;","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/13422","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=13422"}],"version-history":[{"count":7,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/13422\/revisions"}],"predecessor-version":[{"id":14175,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/13422\/revisions\/14175"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/13482"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=13422"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/categories?post=13422"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/tags?post=13422"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}