{"id":11594,"date":"2024-09-25T14:59:38","date_gmt":"2024-09-25T07:29:38","guid":{"rendered":"https:\/\/abtvn.com\/?p=11594"},"modified":"2025-08-06T12:04:01","modified_gmt":"2025-08-06T04:34:01","slug":"tieu-chuan-thiet-ke-va-dau-do-cho-real-time-pcr","status":"publish","type":"post","link":"https:\/\/abtvn.com\/en\/tieu-chuan-thiet-ke-va-dau-do-cho-real-time-pcr\/","title":{"rendered":"<strong>Ti\u00eau chu\u1ea9n thi\u1ebft k\u1ebf m\u1ed3i v\u00e0 \u0111\u1ea7u d\u00f2 cho Real-time PCR \u0111\u1ecbnh l\u01b0\u1ee3ng<\/strong>"},"content":{"rendered":"<p class=\"wp-block-paragraph\"><strong><a href=\"https:\/\/abtvn.com\/en\/phan-tich-ket-qua-real-time-pcr\/\" data-type=\"link\" data-id=\"https:\/\/abtvn.com\/phan-tich-ket-qua-real-time-pcr\/\">Real-time PCR \u0111\u1ecbnh l\u01b0\u1ee3ng (qPCR)<\/a><\/strong> l\u00e0 k\u1ef9 thu\u1eadt \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng ph\u1ed5 bi\u1ebfn trong c\u00e1c l\u0129nh v\u1ef1c x\u00e9t nghi\u1ec7m ch\u1ea9n \u0111o\u00e1n l\u00e2m s\u00e0ng \u0111\u1ec3 \u0111\u1ecbnh l\u01b0\u1ee3ng ch\u00ednh x\u00e1c axit nucleic m\u1ee5c ti\u00eau c\u00f3 trong m\u1eabu. \u0110\u1ec3 th\u1ef1c hi\u1ec7n x\u00e9t nghi\u1ec7m Real-time PCR th\u00e0nh c\u00f4ng, qu\u00e1 tr\u00ecnh khu\u1ebfch \u0111\u1ea1i s\u1ea3n ph\u1ea9m ph\u1ea3i hi\u1ec7u qu\u1ea3 v\u00e0 c\u00f3 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u cao. C\u1ea3 m\u1ed3i v\u00e0 \u0111\u1ea7u d\u00f2 \u0111\u1ec1u c\u00f3 th\u1ec3 \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn hi\u1ec7u qu\u1ea3 v\u00e0 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u c\u1ee7a qu\u00e1 tr\u00ecnh khu\u1ebfch \u0111\u1ea1i v\u00e0 do \u0111\u00f3 \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn \u0111\u1ed9 ch\u00ednh x\u00e1c c\u1ee7a x\u00e9t nghi\u1ec7m Real-time PCR. V\u00ec v\u1eady, c\u1ea7n ph\u1ea3i l\u1ef1a ch\u1ecdn k\u1ef9 l\u01b0\u1ee1ng tr\u00ecnh t\u1ef1 m\u1ee5c ti\u00eau, thi\u1ebft k\u1ebf m\u1ed3i v\u00e0 \u0111\u1ea7u d\u00f2 m\u1ed9t c\u00e1ch c\u1ea9n th\u1eadn v\u00e0 ch\u00ednh x\u00e1c \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o k\u1ebft qu\u1ea3 real-time PCR \u0111\u1ea1t \u0111\u1ed9 ch\u00ednh x\u00e1c.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Khi thi\u1ebft k\u1ebf m\u1ed3i v\u00e0 \u0111\u1ea7u d\u00f2 cho x\u00e9t nghi\u1ec7m Real-time PCR \u0111\u1ecbnh l\u01b0\u1ee3ng c\u1ea7n th\u1ef1c hi\u1ec7n theo c\u00e1c b\u01b0\u1edbc sau:<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full\"><img fetchpriority=\"high\" decoding=\"async\" width=\"503\" height=\"703\" src=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/09\/image-4.png\" alt=\"H\u00ecnh 1. S\u01a1 \u0111\u1ed3 quy tr\u00ecnh chung \u0111\u1ec3 thi\u1ebft k\u1ebf v\u00e0 \u0111\u00e1nh gi\u00e1 c\u00e1c m\u1ed3i v\u00e0 \u0111\u1ea7u d\u00f2 cho ph\u1ea3n \u1ee9ng real-time PCR \u0111\u1ecbnh l\u01b0\u1ee3ng.\" class=\"wp-image-11595\" srcset=\"https:\/\/abtvn.com\/wp-content\/uploads\/2024\/09\/image-4.png 503w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/09\/image-4-215x300.png 215w, https:\/\/abtvn.com\/wp-content\/uploads\/2024\/09\/image-4-9x12.png 9w\" sizes=\"(max-width: 503px) 100vw, 503px\" \/><figcaption class=\"wp-element-caption\"><em><strong>Figure 1. <\/strong>S\u01a1 \u0111\u1ed3 quy tr\u00ecnh chung \u0111\u1ec3 thi\u1ebft k\u1ebf v\u00e0 \u0111\u00e1nh gi\u00e1 c\u00e1c m\u1ed3i v\u00e0 \u0111\u1ea7u d\u00f2 cho ph\u1ea3n \u1ee9ng real-time PCR \u0111\u1ecbnh l\u01b0\u1ee3ng<\/em>.<\/figcaption><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>X\u00e1c \u0111\u1ecbnh tr\u00ecnh t\u1ef1 m\u1ee5c ti\u00eau<\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Tr\u01b0\u1edbc khi ti\u1ebfn h\u00e0nh thi\u1ebft k\u1ebf m\u1ed3i\/\u0111\u1ea7u d\u00f2 cho ph\u1ea3n \u1ee9ng real-time PCR c\u1ea7n x\u00e1c \u0111\u1ecbnh tr\u00ecnh t\u1ef1 m\u1ee5c ti\u00eau c\u1ee5 th\u1ec3 c\u1ea7n khu\u1ebfch \u0111\u1ea1i. Tr\u00ecnh t\u1ef1 m\u1ee5c ti\u00eau n\u00e0y c\u1ea7n c\u00f3 s\u1ef1 \u1ed5n \u0111\u1ecbnh di truy\u1ec1n, \u0111\u1ed9 b\u1ea3o t\u1ed3n cao v\u00e0 c\u00f3 t\u00ednh chuy\u00ean bi\u1ec7t so v\u1edbi tr\u00ecnh t\u1ef1 c\u1ee7a vi sinh v\u1eadt kh\u00e1c.&nbsp; Sau khi l\u1ef1a ch\u1ecdn tr\u00ecnh t\u1ef1 m\u1ee5c ti\u00eau \u0111\u00e3 ho\u00e0n t\u1ea5t, b\u01b0\u1edbc ti\u1ebfp theo l\u00e0 t\u00ecm c\u00e1c v\u00f9ng nh\u1eafm m\u1ee5c ti\u00eau b\u1eb1ng c\u00e1ch th\u1ee7 c\u00f4ng c\u0103n ch\u1ec9nh tr\u00ecnh t\u1ef1 ho\u1eb7c x\u1ebfp gi\u00f3ng c\u1ed9t t\u1ef1 \u0111\u1ed9ng b\u1eb1ng c\u00e1c ph\u1ea7n m\u1ec1m chuy\u00ean d\u1ee5ng.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">M\u1ed9t s\u1ed1 h\u01b0\u1edbng d\u1eabn \u0111\u1ec3 l\u1ef1a ch\u1ecdn tr\u00ecnh t\u1ef1 c\u1ea7n khu\u1ebfch \u0111\u1ea1i ph\u00f9 h\u1ee3p bao g\u1ed3m:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>S\u1ea3n ph\u1ea9m khu\u1ebfch \u0111\u1ea1i c\u00f3 k\u00edch th\u01b0\u1edbc t\u1eeb 75 \u2013 200 bp.<\/li>\n\n\n\n<li>Tr\u00e1nh thi\u1ebft k\u1ebf v\u00e0o c\u00e1c v\u00f9ng c\u00f3 c\u1ea5u tr\u00fac th\u1ee9 c\u1ea5p.<\/li>\n\n\n\n<li>Tr\u00e1nh c\u00e1c v\u00f9ng c\u00f3 c\u00e1c \u0111o\u1ea1n l\u1eb7p l\u1ea1i d\u00e0i (&gt;4) c\u1ee7a c\u00e1c nucleotide.<\/li>\n\n\n\n<li>Ch\u1ecdn v\u00f9ng c\u00f3 h\u00e0m l\u01b0\u1ee3ng GC t\u1eeb 50\u201360%, tr\u00e1nh c\u00e1c v\u00f9ng gi\u00e0u GC trong tr\u00ecnh t\u1ef1 m\u1ee5c ti\u00eau v\u00ec ch\u00fang kh\u00f3 khu\u1ebfch \u0111\u1ea1i.<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">C\u00f3 m\u1ed9t s\u1ed1 t\u00e0i c\u00f4ng c\u1ee5 tr\u1ef1c tuy\u1ebfn mi\u1ec5n ph\u00ed gi\u00fap x\u00e1c \u0111\u1ecbnh tr\u00ecnh t\u1ef1 m\u1ee5c ti\u00eau nh\u01b0 NCBI (<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene?cmd=search\" target=\"_blank\" rel=\"noopener\">https:\/\/www.ncbi.nlm.nih.gov\/gene?cmd=search<\/a>), Vi\u1ec7n Sanger\/Vi\u1ec7n tin sinh Ch\u00e2u \u00c2u (<a href=\"https:\/\/asia.ensembl.org\/index.html\" target=\"_blank\" rel=\"noopener\">https:\/\/asia.ensembl.org\/index.html<\/a>) ho\u1eb7c tham kh\u1ea3o t\u1eeb c\u00e1c nghi\u00ean c\u1ee9u khoa h\u1ecdc \u0111\u00e3 c\u00f4ng b\u1ed1 \u0111\u1ec3 t\u00ecm ki\u1ebfm c\u00e1c gene m\u1ee5c ti\u00eau ti\u1ec1m n\u0103ng.<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>Nguy\u00ean t\u1eafc thi\u1ebft k\u1ebf m\u1ed3i cho cho ph\u1ea3n \u1ee9ng Real-time PCR \u0111\u1ecbnh l\u01b0\u1ee3ng<\/strong><\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u0110\u1ed9 d\u00e0i c\u1ee7a m\u1ed3i:<\/strong> K\u00edch th\u01b0\u1edbc t\u1ed1i \u01b0u c\u1ee7a c\u00e1c \u0111o\u1ea1n m\u1ed3i th\u01b0\u1eddng n\u1eb1m trong kho\u1ea3ng t\u1eeb <strong>15 \u0111\u1ebfn 30 nucleotide<\/strong>. Thi\u1ebft k\u1ebf m\u1ed3i qu\u00e1 ng\u1eafn, l\u00e0m gi\u1ea3m \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u c\u1ee7a ph\u1ea3n \u1ee9ng real-time PCR. Ng\u01b0\u1ee3c l\u1ea1i, m\u1ed3i d\u00e0i c\u00f3 th\u1ec3 li\u00ean k\u1ebft t\u1ed1t v\u1edbi tr\u00ecnh t\u1ef1 m\u1ee5c ti\u00eau, nh\u01b0ng qu\u00e1 d\u00e0i l\u00e0m gi\u1ea3m hi\u1ec7u qu\u1ea3 khu\u1ebfch \u0111\u1ea1i PCR d\u1eabn \u0111\u1ebfn gi\u1ea3m n\u0103ng su\u1ea5t v\u00e0 ch\u1ea5t l\u01b0\u1ee3ng c\u1ee7a s\u1ea3n ph\u1ea9m real-time PCR \u0111\u1ecbnh l\u01b0\u1ee3ng.<\/li>\n\n\n\n<li><strong>Nhi\u1ec7t \u0111\u1ed9 n\u00f3ng ch\u1ea3y c\u1ee7a m\u1ed3i Tm:<\/strong> L\u00e0 th\u00f4ng s\u1ed1 r\u1ea5t quan tr\u1ecdng \u1edf giai \u0111o\u1ea1n g\u1eafn m\u1ed3i. Tm \u0111\u01b0\u1ee3c \u0111\u1ecbnh ngh\u0129a l\u00e0 \u0111i\u1ec3m nhi\u1ec7t t\u1ea1i \u0111\u00f3 50% s\u1ee3i \u0111\u00f4i ADN ph\u00e2n t\u00e1ch nhau t\u1ea1o s\u1ee3i \u0111\u01a1n. Gi\u00e1 tr\u1ecb Tm c\u1ee7a c\u00e1c \u0111o\u1ea1n m\u1ed3i n\u00ean n\u1eb1m trong kho\u1ea3ng t\u1eeb <strong>55 \u0111\u1ebfn 60\u00b0C<\/strong> . Ngo\u00e0i ra, Tm gi\u1eefa m\u1ed3i xu\u00f4i v\u00e0 m\u1ed3i ng\u01b0\u1ee3c kh\u00f4ng \u0111\u01b0\u1ee3c l\u1ec7ch nhau h\u01a1n <strong>2\u00b0C.<\/strong><\/li>\n\n\n\n<li><strong>Nhi\u1ec7t \u0111\u1ed9 g\u1eafn m\u1ed3i (Ta):<\/strong><strong> <\/strong>L\u00e0 nhi\u1ec7t \u0111\u1ed9 m\u1ed3i g\u1eafn l\u00ean DNA khu\u00f4n, \u0111\u01b0\u1ee3c t\u00ednh to\u00e1n d\u1ef1a v\u00e0o nhi\u1ec7t \u0111\u1ed9 n\u00f3ng ch\u1ea3y Tm c\u1ee7a m\u1ed3i. Ta th\u01b0\u1eddng th\u1ea5p h\u01a1n Tm kho\u1ea3ng <strong>3-5<sup>o<\/sup>C<\/strong>. Ta qu\u00e1 cao s\u1ebd t\u1ea1o ra qu\u00e1 tr\u00ecnh b\u1eaft c\u1eb7p b\u1ed5 sung gi\u1eefa m\u1ed3i v\u00e0 m\u1eabu kh\u00f4ng \u0111\u1ee7 d\u1eabn \u0111\u1ebfn n\u1ed3ng \u0111\u1ed9 s\u1ea3n ph\u1ea9m PCR th\u1ea5p. Ta qu\u00e1 th\u1ea5p c\u00f3 th\u1ec3 d\u1eabn \u0111\u1ebfn c\u00e1c s\u1ea3n ph\u1ea9m kh\u00f4ng \u0111\u1eb7c hi\u1ec7u.<\/li>\n\n\n\n<li><strong>H\u00e0m l\u01b0\u1ee3ng GC: <\/strong>Thi\u1ebft k\u1ebf c\u00e1c \u0111o\u1ea1n m\u1ed3i c\u00f3 h\u00e0m l\u01b0\u1ee3ng GC t\u1eeb 50\u201360%. Tr\u00e1nh c\u00e1c v\u00f9ng nhi\u1ec1u nu C v\u00e0 G trong c\u00e1c \u0111o\u1ea1n m\u1ed3i v\u00ec ch\u00fang c\u00f3 th\u1ec3 t\u1ea1o n\u00ean c\u1ea5u tr\u00fac tetraplex, r\u1ea5t \u1ed5n \u0111\u1ecbnh v\u00e0 kh\u00f4ng th\u1ec3 \u0111\u01b0\u1ee3c phi\u00ean m\u00e3 b\u1edfi polymerase.<\/li>\n\n\n\n<li><strong>K\u1eb9p GC:<\/strong> Ch\u1ecdn c\u00e1c \u0111o\u1ea1n m\u1ed3i ch\u1ec9 c\u00f3 m\u1ed9t ho\u1eb7c hai nu G\/C trong n\u0103m nucleotide cu\u1ed1i c\u00f9ng \u1edf \u0111\u1ea7u 3\u2032. H\u00e0m l\u01b0\u1ee3ng GC qu\u00e1 cao \u1edf \u0111\u1ea7u 3\u2019 c\u1ee7a m\u1ed3i c\u00f3 th\u1ec3 g\u00e2y li\u00ean k\u1ebft \u0111\u1ea7u 3\u2019 qu\u00e1 m\u1ea1nh khi\u1ebfn m\u1ed3i b\u1eaft c\u1eb7p kh\u00f4ng \u0111\u1eb7c hi\u1ec7u l\u00e0m gi\u1ea3m t\u00ednh \u0111\u1eb7c hi\u1ec7u c\u1ee7a ph\u1ea3n \u1ee9ng. M\u1ed3i n\u00ean c\u00f3 \u00edt nh\u1ea5t m\u1ed9t G ho\u1eb7c C \u1edf \u0111\u1ea7u 3\u2032 \u0111\u1ea3m b\u1ea3o li\u00ean k\u1ebft ch\u00ednh x\u00e1c v\u00e0 m\u1ea1nh v\u1edbi khu\u00f4n m\u1eabu.<\/li>\n\n\n\n<li><strong>Tr\u00e1nh c\u1ea5u tr\u00fac th\u1ee9 c\u1ea5p:<\/strong> \u0110\u00e2y l\u00e0 m\u1ed9t hi\u1ec7n t\u01b0\u1ee3ng ph\u1ed5 bi\u1ebfn trong ph\u1ea3n \u1ee9ng real-time PCR khi hai m\u1ed3i t\u1ef1 li\u00ean k\u1ebft v\u1edbi nhau thay v\u00ec v\u1edbi khu\u00f4n m\u1eabu, \u0111\u1eb7c bi\u1ec7t l\u00e0 g\u1ea7n \u0111\u1ea7u 3\u2032.&nbsp; C\u00e1c c\u1ea5u tr\u00fac th\u1ee9 c\u1ea5p nh\u01b0 k\u1eb9p t\u00f3c, primer-dimer, t\u01b0\u01a1ng \u0111\u1ed3ng ch\u00e9o, l\u1eb7p c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c h\u00ecnh th\u00e0nh v\u00e0 g\u00e2y ra hi\u1ec7n t\u01b0\u1ee3ng d\u01b0\u01a1ng t\u00ednh gi\u1ea3 ho\u1eb7c \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn c\u01b0\u1eddng \u0111\u1ed9 t\u00edn hi\u1ec7u k\u1ebft qu\u1ea3, v\u00ec v\u1eady c\u1ea7n tr\u00e1nh t\u1ea1o c\u00e1c c\u1ea5u tr\u00fac n\u00e0y nh\u1ea5t c\u00f3 th\u1ec3 khi thi\u1ebft k\u1ebf.<\/li>\n<\/ul>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>Nguy\u00ean t\u1eafc thi\u1ebft k\u1ebf \u0111\u1ea7u d\u00f2 cho ph\u1ea3n \u1ee9ng real-time PCR \u0111\u1ecbnh l\u01b0\u1ee3ng<\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/abtvn.com\/en\/phan-tich-ket-qua-real-time-pcr\/\">\u0110\u1ea7u d\u00f2 TaqMan<\/a>&nbsp;l\u00e0 m\u1ed9t lo\u1ea1i \u0111\u1ea7u d\u00f2 \u0111\u00e1nh d\u1ea5u hu\u1ef3nh quang ph\u1ed5 bi\u1ebfn s\u1eed d\u1ee5ng trong Real-time PCR \u0111\u1ec3 ph\u00e1t hi\u1ec7n v\u00e0 \u0111\u1ecbnh l\u01b0\u1ee3ng m\u1ed9t ho\u1eb7c nhi\u1ec1u t\u00e1c nh\u00e2n m\u1ee5c ti\u00eau c\u00f3 trong m\u1eabu. Khi thi\u1ebft k\u1ebf \u0111\u1ea7u d\u00f2 TaqMan c\u1ea7n l\u01b0u \u00fd c\u00e1c \u0111\u1eb7c \u0111i\u1ec3m sau:<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>\u0110\u1ed9 d\u00e0i c\u1ee7a \u0111\u1ea7u d\u00f2:<\/strong> Chi\u1ec1u d\u00e0i \u0111\u1ea7u d\u00f2 n\u00ean t\u1eeb <strong>18-30 nucleotide<\/strong> v\u00e0 t\u1ed1i \u01b0u nh\u1ea5t \u1edf 23 nu. Vi\u1ec7c s\u1eed d\u1ee5ng \u0111\u1ea7u d\u00f2 qu\u00e1 d\u00e0i \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn \u0111\u1ed9 nh\u1ea1y,\u00a0 s\u1ef1 d\u1eadp t\u1eaft t\u00edn hi\u1ec7u hu\u1ef3nh quang k\u00e9m hi\u1ec7u qu\u1ea3 h\u01a1n t\u1ea1o ra t\u00edn hu\u1ef3nh quang th\u1ea5p. Trong khi vi\u1ec7c s\u1eed d\u1ee5ng c\u00e1c \u0111\u1ea7u d\u00f2 qu\u00e1 ng\u1eafn l\u00e0m gi\u1ea3m \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u c\u1ee7a ph\u1ea3n \u1ee9ng.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Nhi\u1ec7t \u0111\u1ed9 n\u00f3ng ch\u1ea3y c\u1ee7a \u0111\u1ea7u d\u00f2 Tm:<\/strong> Tm c\u1ee7a \u0111\u1ea7u d\u00f2 ph\u1ea3i cao <strong>h\u01a1n 8\u201310\u00b0C<\/strong> so v\u1edbi c\u00e1c \u0111o\u1ea1n m\u1ed3i v\u00ec \u0111\u1ea7u d\u00f2 s\u1ebd li\u00ean k\u1ebft v\u1edbi m\u1ea1ch khu\u00f4n tr\u01b0\u1edbc c\u00e1c m\u1ed3i xu\u00f4i v\u00e0 m\u1ed3i ng\u01b0\u1ee3c trong qu\u00e1 tr\u00ecnh khu\u1ebfch \u0111\u1ea1i.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>H\u00e0m l\u01b0\u1ee3ng GC:<\/strong> T\u1ef7 l\u1ec7 GC t\u1eeb <strong>30 \u0111\u1ebfn 80%.<\/strong> Nucleotide \u0111\u1ea7u ti\u00ean \u1edf \u0111\u1ea7u 5\u2032 c\u1ee7a \u0111\u1ea7u d\u00f2 kh\u00f4ng thi\u1ebft k\u1ebf nu G \u0111\u1ec3 tr\u00e1nh hi\u1ec7n t\u01b0\u1ee3ng d\u1eadp t\u1eaft t\u00edn hi\u1ec7u ngay c\u1ea3 sau khi \u0111\u1ea7u d\u00f2 ph\u00e2n c\u1eaft, d\u1eabn \u0111\u1ebfn gi\u1ea3m gi\u00e1 tr\u1ecb t\u00edn hi\u1ec7u hu\u1ef3nh quang. H\u01a1n n\u1eefa, \u0111\u1ea7u d\u00f2 ph\u1ea3i ch\u1ee9a nhi\u1ec1u C h\u01a1n G cho ph\u00e9p c\u00e1c t\u00edn hi\u1ec7u d\u01b0\u01a1ng t\u00ednh th\u1ea5p c\u00f3 th\u1ec3 d\u1ec5 d\u00e0ng ph\u00e2n bi\u1ec7t h\u01a1n v\u1edbi t\u00edn hi\u1ec7u n\u1ec1n<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>C\u1ea5u tr\u00fac th\u1ee9 c\u1ea5p<\/strong>: \u0110\u1ea7u d\u00f2 c\u1ea7n tr\u00e1nh c\u1ea5u tr\u00fac th\u1ee9 c\u1ea5p, ngo\u00e0i ra kh\u00f4ng \u0111\u01b0\u1ee3c ch\u1ed3ng l\u00ean ho\u1eb7c b\u1ed5 sung cho b\u1ea5t k\u1ef3 \u0111o\u1ea1n m\u1ed3i n\u00e0o.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>L\u1ef1a ch\u1ecdn Reporter v\u00e0 quencher th\u00edch h\u1ee3p:<\/strong>&nbsp; L\u1ef1a ch\u1ecdn reporter v\u00e0 quencher c\u1ea7n xem x\u00e9t ba ti\u00eau ch\u00ed: (a) c\u00e1c \u0111\u1ea7u d\u00f2 ph\u1ea3i \u0111\u01b0\u1ee3c g\u1eafn reporter c\u00f3 ph\u1ed5 hu\u1ef3nh quang t\u00e1ch bi\u1ec7t t\u1ed1t ho\u1eb7c ch\u1ec9 c\u00f3 s\u1ef1 ch\u1ed3ng ch\u00e9o t\u1ed1i thi\u1ec3u, (b) &nbsp;k\u1ebft h\u1ee3p reporter v\u00e0 quencher ph\u1ea3i t\u01b0\u01a1ng th\u00edch v\u1edbi kh\u1ea3 n\u0103ng ph\u00e1t hi\u1ec7n c\u1ee7a thi\u1ebft b\u1ecb real-time PCR v\u00e0 (c) n\u00ean s\u1eed d\u1ee5ng quencher d\u1ea1ng kh\u00f4ng hu\u1ef3nh quang. Tham kh\u1ea3o c\u00e1c ch\u1ea5t reporter v\u00e0 quencher t\u1ea1i b\u00e0i vi\u1ebft <a href=\"https:\/\/abtvn.com\/en\/vi-sao-lua-chon-taqman-probe-cho-real-time-pcr\/\">V\u00ec sao l\u1ef1a ch\u1ecdn Taqman probe cho Real-time PCR?<\/a><\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"wp-block-paragraph\">C\u00e1c th\u00f4ng s\u1ed1 ti\u00eau chu\u1ea9n khi thi\u1ebft k\u1ebf m\u1ed3i\/\u0111\u1ea7u d\u00f2 cho ph\u1ea3n \u1ee9ng real-time PCR \u0111\u01b0\u1ee3c t\u00f3m t\u1eaft trong b\u1ea3ng sau:<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td><strong>Y\u1ebfu t\u1ed1<\/strong><\/td><td><strong>Primers<\/strong><\/td><td><strong>Probes<\/strong><\/td><\/tr><tr><td><strong>Chi\u1ec1u d\u00e0i<\/strong><strong><\/strong><\/td><td>Chi\u1ec1u&nbsp;d\u00e0i&nbsp;15-30 Nu v\u00e0 \u0111\u1ed9 d\u00e0i t\u1ed1i \u01b0u l\u00e0 kho\u1ea3ng t\u1eeb 18-22 nu<\/td><td>Chi\u1ec1u&nbsp;d\u00e0i&nbsp;18-30&nbsp;Nu v\u00e0 t\u1ed1i \u01b0u nh\u1ea5t \u1edf 23 nu.<\/td><\/tr><tr><td><strong>%GC<\/strong><strong><\/strong><\/td><td>Dao \u0111\u1ed9ng t\u1eeb 50-60%<\/td><td>Dao \u0111\u1ed9ng t\u1eeb&nbsp; 30-80%<\/td><\/tr><tr><td><strong>\u0110\u1eb7c&nbsp;\u0111i\u1ec3m&nbsp;tr\u00ecnh&nbsp;t\u1ef1<\/strong><strong><\/strong><\/td><td>Kh\u00f4ng&nbsp;n\u00ean&nbsp;\u0111\u1ec3&nbsp;li\u00ean&nbsp;ti\u1ebfp&nbsp;3 nu GGG li\u00ean ti\u1ebfp nhau. M\u1ed3i xu\u00f4i v\u00e0 m\u1ed3i ng\u01b0\u1ee3c kh\u00f4ng c\u00e1ch qu\u00e1 xa nhau. 5 nu \u1edf \u0111\u1ea7u k\u1ebft th\u00fac 3\u2019 kh\u00f4ng nhi\u1ec1u h\u01a1n 2 G,C<\/td><td>Kh\u00f4ng&nbsp;n\u00ean&nbsp;\u0111\u1ec3&nbsp;li\u00ean&nbsp;ti\u1ebfp&nbsp;3 nu GGG li\u00ean ti\u1ebfp nhau. Kh\u00f4ng n\u00ean c\u00f3 G \u1edf \u0111\u1ea7u k\u1ebft th\u00fac 5\u2019 Ch\u1ee9a nhi\u1ec1u C h\u01a1n G<\/td><\/tr><tr><td><strong>Nhi\u1ec7t&nbsp;\u0111\u1ed9&nbsp;n\u00f3ng&nbsp;ch<\/strong><strong>\u1ea3y (Tm)<\/strong><strong><\/strong><\/td><td>N\u1eb1m trong kho\u1ea3ng t\u1eeb&nbsp;50-60\u2103. Tm gi\u1eefa m\u1ed3i xu\u00f4i v\u00e0 m\u1ed3i ng\u01b0\u1ee3c kh\u00f4ng \u0111\u01b0\u1ee3c l\u1ec7ch nhau h\u01a1n 2\u00b0C.<\/td><td>Tm c\u1ee7a probe cao\u00a0h\u01a1n\u00a0m\u1ed3i\u00a08-10\u2103<\/td><\/tr><tr><td><strong>C\u1ea5u tr\u00fac th\u1ee9 c\u1ea5p<\/strong><strong><\/strong><\/td><td colspan=\"2\">H\u1ea1n ch\u1ebf h\u00ecnh th\u00e0nh c\u1ea5u tr\u00fac th\u1ee9 c\u1ea5p \u0394G&nbsp;&gt;&nbsp;-9kcalo\/mol<\/td><\/tr><tr><td><strong>Specificity<\/strong><strong><\/strong><\/td><td colspan=\"2\">Kh\u1ea3 n\u0103ng b\u1eaft c\u1eb7p chuy\u00ean bi\u1ec7t l\u00ean tr\u00ecnh t\u1ef1 m\u1ee5c ti\u00eau quan t\u00e2m<\/td><\/tr><\/tbody><\/table><figcaption class=\"wp-element-caption\"><em><strong>B\u1ea3ng 1. <\/strong>C\u00e1c th\u00f4ng s\u1ed1 c\u1ea7n quan t\u00e2m khi thi\u1ebft k\u1ebf m\u1ed3i\/\u0111\u1ea7u d\u00f2 cho ph\u1ea3n \u1ee9ng real-time PCR<\/em><\/figcaption><\/figure>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>C\u00e1c ph\u1ea7n m\u1ec1m h\u1ed7 tr\u1ee3 thi\u1ebft k\u1ebf m\u1ed3i v\u00e0 \u0111\u1ea7u d\u00f2<\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Thi\u1ebft k\u1ebf m\u1ed3i v\u00e0 \u0111\u1ea7u d\u00f2 trong th\u1ef1c t\u1ebf c\u00f3 th\u1ec3 th\u1ef1c hi\u1ec7n b\u1eb1ng c\u00e1ch th\u1ee7 c\u00f4ng, tuy nhi\u00ean vi\u1ec7c n\u00e0y t\u1ed1n nhi\u1ec1u th\u1eddi gian th\u1ef1c hi\u1ec7n v\u00ec c\u00f3 nhi\u1ec1u th\u00f4ng s\u1ed1 c\u1ea7n t\u00ednh to\u00e1n. Do \u0111\u00f3, hi\u1ec7n nay c\u00f3 nhi\u1ec1u c\u00f4ng c\u1ee5 tin sinh h\u1ecdc tr\u1ef1c tuy\u1ebfn v\u00e0 th\u01b0\u01a1ng m\u1ea1i \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng th\u01b0\u1eddng xuy\u00ean \u0111\u1ec3 thi\u1ebft k\u1ebf m\u1ed3i\/\u0111\u1ea7u d\u00f2 \u0111\u1eb7c hi\u1ec7u, \u0111\u1ea1t hi\u1ec7u qu\u1ea3 khu\u1ebfch \u0111\u1ea1i qPCR, kh\u1ea3 n\u0103ng t\u00e1i t\u1ea1o v\u00e0 \u0111\u1ed9 nh\u1ea1y cao h\u01a1n.<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\" style=\"font-size:25px\"><strong>M\u1ed9t s\u1ed1 c\u00f4ng c\u1ee5 ph\u00e2n t\u00edch tr\u00ecnh t\u1ef1 v\u00e0 thi\u1ebft k\u1ebf m\u1ed3i ph\u1ed5 bi\u1ebfn:<\/strong><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Clustal:&nbsp; S\u1eafp gi\u00f3ng c\u1ed9t c\u00e1c tr\u00ecnh t\u1ef1<\/li>\n\n\n\n<li>Annhyb: Ph\u00e2n t\u00edch tr\u00ecnh t\u1ef1<\/li>\n\n\n\n<li>Bioedit: Thi\u1ebft k\u1ebf m\u1ed3i, ph\u00e2n t\u00edch tr\u00ecnh t\u1ef1<\/li>\n\n\n\n<li><a href=\"http:\/\/frodo.wi.mit.edu\/primer3\/\" target=\"_blank\" rel=\"noopener\">Primer3 (Whitehead Institute, MIT)<\/a><\/li>\n\n\n\n<li><a href=\"http:\/\/bibiserv.techfak.uni-bielefeld.de\/genefisher\/\" target=\"_blank\" rel=\"noopener\">GeneFisher (Bielefeld University)<\/a><\/li>\n\n\n\n<li><a href=\"http:\/\/primerdigital.com\/fastpcr.html\" target=\"_blank\" rel=\"noopener\">FastPCR (PrimerDigital, Helsinki, Finland)<\/a><\/li>\n\n\n\n<li><a href=\"http:\/\/perlprimer.sourceforge.net\/\" target=\"_blank\" rel=\"noopener\">PerlPrimer (Owen Marshall)<\/a><\/li>\n\n\n\n<li><a href=\"http:\/\/dbb.nhri.org.tw\/primer\/\" target=\"_blank\" rel=\"noopener\">Primer Design Assistant (Division of Biostatistics and Bioinformatics, NHRI)<\/a><\/li>\n\n\n\n<li><a href=\"http:\/\/www.premierbiosoft.com\/molecular_beacons\/index.html\" target=\"_blank\" rel=\"noopener\">Beacon Designer (Premier Biosoft Intl)*<\/a><\/li>\n<\/ul>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\" style=\"font-size:25px\"><strong>C\u00f4ng c\u1ee5 ki\u1ec3m tra \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u c\u1ee7a m\u1ed3i:<\/strong><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/tools\/primer-blast\/index.cgi?LINK_LOC=BlastHome\" target=\"_blank\" rel=\"noopener\">Primer-Blast<\/a> (NCBI)<\/li>\n<\/ul>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\" style=\"font-size:25px\"><strong>C\u00f4ng c\u1ee5 ki\u1ec3m tra m\u1ed3i:<\/strong><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"http:\/\/www.idtdna.com\/analyzer\/Applications\/OligoAnalyzer\/\" target=\"_blank\" rel=\"noopener\">OligoAnalyzer (IDT)<\/a><\/li>\n\n\n\n<li><a href=\"http:\/\/www.premierbiosoft.com\/netprimer\/netprlaunch\/netprlaunch.html\" target=\"_blank\" rel=\"noopener\">NetPrimer (Premier Biosoft)<\/a><\/li>\n\n\n\n<li><a href=\"http:\/\/www.basic.northwestern.edu\/biotools\/oligocalc.html\" target=\"_blank\" rel=\"noopener\">Oligonucleotide Properties Calculator (Northwestern University)<\/a><\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">Thi\u1ebft k\u1ebf t\u1ed1i \u01b0u c\u00e1c \u0111o\u1ea1n m\u1ed3i v\u00e0 \u0111\u1ea7u d\u00f2 cho real-time PCR b\u1eb1ng m\u1ed9t s\u1ed1 c\u00f4ng c\u1ee5 tr\u00ean l\u00e0 \u0111i\u1ec1u c\u1ea7n thi\u1ebft \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o khu\u1ebfch \u0111\u1ea1i hi\u1ec7u qu\u1ea3 v\u00e0 \u0111\u1eb7c hi\u1ec7u c\u00e1c s\u1ea3n ph\u1ea9m khu\u1ebfch \u0111\u1ea1i. Ng\u01b0\u1eddi s\u1eed d\u1ee5ng c\u1ea7n ki\u1ec3m tra k\u1ef9 l\u01b0\u1ee1ng c\u00e1c \u01b0u \u0111i\u1ec3m v\u00e0 nh\u01b0\u1ee3c \u0111i\u1ec3m c\u1ee7a ph\u1ea7n m\u1ec1m tr\u01b0\u1edbc khi l\u1ef1a ch\u1ecdn, \u0111\u1eb7c bi\u1ec7t tr\u00e1nh c\u00e1c \u0111o\u1ea1n m\u1ed3i c\u00f3 c\u1ea5u tr\u00fac th\u1ee9 c\u1ea5p.<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"wp-block-paragraph\">D\u01b0\u1edbi \u0111\u00e2y l\u00e0 m\u1ed9t b\u1ea3ng so s\u00e1nh c\u00e1c c\u00f4ng c\u1ee5 thi\u1ebft k\u1ebf m\u1ed3i v\u00e0 ph\u00e2n t\u00edch oligonucleotide v\u1edbi c\u00e1c ph\u1ea7n m\u1ec1m thi\u1ebft k\u1ebf v\u00e0 ph\u00e2n t\u00edch m\u1ed3i tr\u1ef1c tuy\u1ebfn ph\u1ed5 bi\u1ebfn kh\u00e1c do c\u00f4ng ty cung c\u1ea5p ph\u1ea7n m\u1ec1m jPCR Tool &amp;FastPCR th\u1ed1ng k\u00ea.<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td><strong>\u0110\u1eb7c \u0111i\u1ec3m<\/strong><\/td><td><a href=\"https:\/\/primerdigital.com\/fastpcr.html\" target=\"_blank\" rel=\"noopener\"><strong><br>FastPCR<\/strong><\/a><\/td><td><a href=\"http:\/\/www.ncbi.nlm.nih.gov\/tools\/primer-blast\/\" target=\"_blank\" rel=\"noopener\"><strong>NCBI\/Primer-BLAST (Primer3)<\/strong><\/a><\/td><td><a href=\"http:\/\/batchprimer3.bioinformatics.ucdavis.edu\/cgi-bin\/batchprimer3\/batchprimer3.cgi\" target=\"_blank\" rel=\"noopener\"><strong>BatchPrimer3 (Primer3)<\/strong><\/a><\/td><td><a href=\"http:\/\/www.idtdna.com\/pages\/scitools\" target=\"_blank\" rel=\"noopener\"><strong>IDT SciTools: PrimerQuest, OligoAnalyzer 3.1<\/strong><\/a><\/td><td><a href=\"http:\/\/perlprimer.sourceforge.net\/\" target=\"_blank\" rel=\"noopener\"><strong>PerlPrimer<\/strong><\/a><\/td><td><a href=\"http:\/\/bisearch.enzim.hu\/\" target=\"_blank\" rel=\"noopener\"><strong>BiSearch Web server<\/strong><\/a><\/td><td><a href=\"http:\/\/www.changbioscience.com\/primo\/\" target=\"_blank\" rel=\"noopener\"><strong>Primo Pro 3.4<\/strong><\/a><\/td><\/tr><tr><td>\u0110\u1ed9 d\u00e0i m\u1ed3i\/\u0111\u1ea7u d\u00f2 (nt)<\/td><td>12-500<\/td><td>15-30<\/td><td>&nbsp;<\/td><td>16-35<\/td><td>12-30<\/td><td>10-35<\/td><td>13-36<\/td><\/tr><tr><td>Gi\u1edbi h\u1ea1n \u0111\u1ed9 d\u00e0i khu\u00f4n m\u1eabu (nt)<\/td><td>Kh\u00f4ng gi\u1edbi h\u1ea1n<\/td><td>50,000<\/td><td>&nbsp;<\/td><td>Kh\u00f4ng gi\u1edbi h\u1ea1n<\/td><td>Kh\u00f4ng gi\u1edbi h\u1ea1n<\/td><td>5000<\/td><td>Kh\u00f4ng gi\u1edbi h\u1ea1n<\/td><\/tr><tr><td>T\u1ed1c \u0111\u1ed9 t\u00ednh to\u00e1n<\/td><td>R\u1ea5t nhanh<\/td><td>Ch\u1eadm<\/td><td>Ch\u1eadm<\/td><td>Ch\u1eadm<\/td><td>Ch\u1eadm<\/td><td>R\u1ea5t ch\u1eadm<\/td><td>Nhanh<\/td><\/tr><tr><td>Cho ph\u00e9p ch\u1ea1y th\u00f4ng l\u01b0\u1ee3ng cao v\u1edbi nhi\u1ec1u m\u1eabu v\u00e0 nhi\u1ec1u m\u1ee5c ti\u00eau tr\u00ean khu\u00f4n c\u00f9ng l\u00fac<\/td><td>Yes<\/td><td>No<\/td><td>Yes<\/td><td>No<\/td><td>No<\/td><td>No<\/td><td>Yes<\/td><\/tr><tr><td>T\u00f9y ch\u1ecdn PCR cho khu\u00f4n m\u1eabu<\/td><td>Yes<\/td><td>Yes<\/td><td>Yes<\/td><td>Yes<\/td><td>Yes<\/td><td>Yes<\/td><td>Yes<\/td><\/tr><tr><td>C\u00e1c nucleotide tho\u00e1i h\u00f3a (t\u00ednh to\u00e1n Tm, t\u00ecm ki\u1ebfm v\u00e0 thi\u1ebft k\u1ebf m\u1ed3i, v.v.)<\/td><td>Yes<\/td><td>No<\/td><td>Yes<\/td><td>Yes<\/td><td>No<\/td><td>Yes<\/td><td>No<\/td><\/tr><tr><td>Bi\u1ebfn \u0111\u1ed5i LNA v\u00e0 nucleotide\u00a0 kh\u00e1c<\/td><td>Yes<\/td><td>No<\/td><td>No<\/td><td>Yes<\/td><td>No<\/td><td>No<\/td><td>No<\/td><\/tr><tr><td>Hi\u1ec7u qu\u1ea3 PCR m\u1ed3i v\u00e0 x\u00e1c \u0111\u1ecbnh t\u00ednh ph\u1ee9c t\u1ea1p.<\/td><td>C\u00f3,<br><strong>LC=91.1\u00b13.6% (6000 primers)<\/strong><\/td><td><a href=\"https:\/\/primerdigital.com\/image\/LC_FastPCR_NCBI.png\" target=\"_blank\" rel=\"noopener\">No<\/a>,<br><strong>LC=<\/strong><a href=\"https:\/\/primerdigital.com\/image\/PQ_FastPCR_NCBI.png\" target=\"_blank\" rel=\"noopener\"><strong>79.6\u00b19.4% (6000 primers)<\/strong><\/a><\/td><td>No<\/td><td>Kh\u00f4ng,<br><strong>LC=84.4\u00b18.1% (407 primers)<\/strong><\/td><td>No<\/td><td>Kh\u00f4ng,<br><a href=\"https:\/\/primerdigital.com\/tools\/lc_error.txt\" target=\"_blank\" rel=\"noopener\"><strong>LC=73.2\u00b110.8% (524 primers)<\/strong><\/a><\/td><td>No<\/td><\/tr><tr><td>T\u00ednh to\u00e1n nhi\u1ec7t \u0111\u1ed9 \u1ee7 t\u1ed1i \u01b0u<\/td><td>Yes<\/td><td>No<\/td><td>No<\/td><td>No<\/td><td>No<\/td><td>No<\/td><td>No<\/td><\/tr><tr><td>Ki\u1ec3m tra \u0111\u1ea7u 3&#8242; c\u1ee7a Primer v\u00e0 t\u1ef1 t\u1ea1o dimer<\/td><td>Yes<\/td><td><a href=\"https:\/\/primerdigital.com\/tools\/blast_primers_dimers.txt\" target=\"_blank\" rel=\"noopener\"><strong>Error<\/strong><\/a><\/td><td>Yes<\/td><td>Yes<\/td><td>Yes<\/td><td>Yes<\/td><td>Yes<\/td><\/tr><tr><td>Ki\u1ec3m tra m\u1ed3i n\u1ed9i giao v\u00e0 t\u1ef1 t\u1ea1p dimer<\/td><td>Yes<\/td><td><a href=\"https:\/\/primerdigital.com\/tools\/blast_primers_dimers.txt\" target=\"_blank\" rel=\"noopener\"><strong>Error<\/strong><\/a><strong><\/strong><\/td><td>No<\/td><td><a href=\"https:\/\/primerdigital.com\/tools\/IDT_primers_dimers.txt\" target=\"_blank\" rel=\"noopener\"><strong>Error<\/strong><\/a><\/td><td>Yes<\/td><td>Yes<\/td><td>No<\/td><\/tr><tr><td>T\u00ecm ki\u1ebfm khu\u00f4n m\u1eabu Blast<\/td><td>No<\/td><td>Yes<strong><\/strong><\/td><td>No<\/td><td>No<\/td><td>Yes<\/td><td>Yes<\/td><td>Yes<\/td><\/tr><tr><td>C\u00e1c th\u00f4ng s\u1ed1 thi\u1ebft k\u1ebf m\u1ed3i \u0111\u01b0\u1ee3c \u0111\u00e1nh gi\u00e1 trong ph\u00f2ng th\u00ed nghi\u1ec7m<\/td><td>Yes<\/td><td>Yes<\/td><td>Yes<\/td><td>Yes<\/td><td>Yes<\/td><td>Yes<\/td><td>Yes<\/td><\/tr><\/tbody><\/table><figcaption class=\"wp-element-caption\"><em><strong>B\u1ea3ng 2. <\/strong>So s\u00e1nh c\u00e1c c\u00f4ng c\u1ee5 thi\u1ebft k\u1ebf m\u1ed3i\/\u0111\u1ea7u d\u00f2 ph\u1ed5 bi\u1ebfn hi\u1ec7n nay<\/em><br><em>*C\u00e1c th\u00f4ng s\u1ed1 so s\u00e1nh ch\u1ec9 mang gi\u00e1 tr\u1ecb tham kh\u1ea3o.<\/em><\/figcaption><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\">Sau khi&nbsp; m\u1ed3i v\u00e0 \u0111\u1ea7u d\u00f2 \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf th\u00e0nh c\u00f4ng c\u1ea7n ki\u1ec3m tra t\u00ednh \u0111\u1eb7c hi\u1ec7u v\u00e0 kh\u1ea3 n\u0103ng khu\u1ebfch \u0111\u1ea1i b\u1eb1ng c\u00e1ch ki\u1ec3m tra in silico v\u00e0 PCR th\u1ef1c nghi\u1ec7m.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Tr\u00ecnh t\u1ef1 m\u1ed3i\/\u0111\u1ea7u d\u00f2 sau khi thi\u1ebft k\u1ebf \u0111\u01b0\u1ee3c g\u1eedi \u0111\u1ebfn c\u00e1c c\u00f4ng ty <strong>d\u1ecbch v\u1ee5 t\u1ed5ng h\u1ee3p oligonucleotide <\/strong>\u0111\u1ec3 ti\u1ebfn h\u00e0nh t\u1ed5ng h\u1ee3p chu\u1ea9n b\u1ecb cho c\u00e1c th\u00ed nghi\u1ec7m. Hi\u1ec7n nay,<strong><a href=\"https:\/\/oligox.abtvn.com\/saner\/\" data-type=\"link\" data-id=\"https:\/\/oligox.abtvn.com\/saner\/\"> OligoX<\/a><\/strong> l\u00e0 th\u01b0\u01a1ng hi\u1ec7u thu\u1ed9c c\u00f4ng ty<strong> ABT<\/strong> ph\u00e1t tri\u1ec3n cung c\u1ea5p c\u00e1c lo\u1ea1i m\u1ed3i\/\u0111\u1ea7u d\u00f2 t\u1ed5ng h\u1ee3p ch\u1ea5t l\u01b0\u1ee3ng cao, \u0111\u00e1p \u1ee9ng \u0111\u1ea7y \u0111\u1ee7 c\u00e1c y\u00eau c\u1ea7u c\u1ee7a kh\u00e1ch h\u00e0ng trong nhi\u1ec1u l\u0129nh v\u1ef1c \u1ee9ng d\u1ee5ng kh\u00e1c nhau, t\u1eeb nghi\u00ean c\u1ee9u khoa h\u1ecdc \u0111\u1ebfn \u1ee9ng d\u1ee5ng y t\u1ebf v\u00e0 c\u00f4ng ngh\u1ec7.<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>T\u00e0i li\u1ec7u kham kh\u1ea3o<\/strong><\/h2>\n\n\n\n<ol start=\"1\" class=\"wp-block-list\">\n<li>Basu, Chhandak. (2015). [Methods in Molecular Biology] PCR Primer Design Volume 1275 || Design of Primers and Probes for Quantitative Real-Time PCR Methods. , 10.1007\/978-1-4939-2365-6(Chapter 3), 31\u201356.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; doi:10.1007\/978-1-4939-2365-6_3&nbsp;<\/li>\n\n\n\n<li>Juntaek Lim; Seung Gu Shin; Seungyong Lee; Seokhwan Hwang. (2011). Design and use of group-specific primers and probes for real-time quantitative PCR. , 5(1), 28\u201339.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; doi:10.1007\/s11783-011-0302-x&nbsp;<\/li>\n\n\n\n<li><a href=\"http:\/\/primerdigital.com\/tools\/soft.html\" target=\"_blank\" rel=\"noopener\"><strong>PrimerDigital<\/strong><\/a>, <a href=\"https:\/\/primerdigital.com\/tools\/soft.html\" target=\"_blank\" rel=\"noopener\">Online PCR primer software comparision (primerdigital.com)<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.bio-rad.com\/en-vn\/applications-technologies\/qpcr-assay-design-optimization?ID=LUSO7RIVK\" target=\"_blank\" rel=\"noopener\"><strong>qPCR Assay Design and Optimization | Bio-Rad<\/strong><\/a><\/li>\n\n\n\n<li>Sinh h\u1ecdc Vi\u1ec7t Nam, (2018), Thi\u1ebft k\u1ebf m\u1ed3i cho ph\u1ea3n \u1ee9ng PCR<\/li>\n<\/ol>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>","protected":false},"excerpt":{"rendered":"<p>Real-time PCR \u0111\u1ecbnh l\u01b0\u1ee3ng (qPCR) l\u00e0 k\u1ef9 thu\u1eadt \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng ph\u1ed5 bi\u1ebfn trong c\u00e1c l\u0129nh v\u1ef1c x\u00e9t nghi\u1ec7m ch\u1ea9n \u0111o\u00e1n l\u00e2m s\u00e0ng \u0111\u1ec3 \u0111\u1ecbnh l\u01b0\u1ee3ng ch\u00ednh x\u00e1c axit nucleic m\u1ee5c ti\u00eau c\u00f3 trong m\u1eabu. \u0110\u1ec3 th\u1ef1c hi\u1ec7n x\u00e9t nghi\u1ec7m Real-time PCR th\u00e0nh c\u00f4ng, qu\u00e1 tr\u00ecnh khu\u1ebfch \u0111\u1ea1i s\u1ea3n ph\u1ea9m ph\u1ea3i hi\u1ec7u qu\u1ea3 v\u00e0 c\u00f3 [&hellip;]<\/p>","protected":false},"author":1,"featured_media":11598,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/admin\/"},"uagb_comment_info":0,"uagb_excerpt":"Real-time PCR \u0111\u1ecbnh l\u01b0\u1ee3ng (qPCR) l\u00e0 k\u1ef9 thu\u1eadt \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng ph\u1ed5 bi\u1ebfn trong c\u00e1c l\u0129nh v\u1ef1c x\u00e9t nghi\u1ec7m ch\u1ea9n \u0111o\u00e1n l\u00e2m s\u00e0ng \u0111\u1ec3 \u0111\u1ecbnh l\u01b0\u1ee3ng ch\u00ednh x\u00e1c axit nucleic m\u1ee5c ti\u00eau c\u00f3 trong m\u1eabu. \u0110\u1ec3 th\u1ef1c hi\u1ec7n x\u00e9t nghi\u1ec7m Real-time PCR th\u00e0nh c\u00f4ng, qu\u00e1 tr\u00ecnh khu\u1ebfch \u0111\u1ea1i s\u1ea3n ph\u1ea9m ph\u1ea3i hi\u1ec7u qu\u1ea3 v\u00e0 c\u00f3&hellip;","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/11594","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=11594"}],"version-history":[{"count":5,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/11594\/revisions"}],"predecessor-version":[{"id":13062,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/11594\/revisions\/13062"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/11598"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=11594"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/categories?post=11594"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/tags?post=11594"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}