{"id":11572,"date":"2024-09-01T08:34:00","date_gmt":"2024-09-01T01:04:00","guid":{"rendered":"https:\/\/abtvn.com\/?p=11572"},"modified":"2025-08-06T12:10:43","modified_gmt":"2025-08-06T04:40:43","slug":"tach-chiet-cot-silica-cho-nen-mau-thu-y","status":"publish","type":"post","link":"https:\/\/abtvn.com\/en\/tach-chiet-cot-silica-cho-nen-mau-thu-y\/","title":{"rendered":"T\u00c1CH CHI\u1ebeT C\u1ed8T SILICA CHO N\u1ec0N M\u1eaaU TH\u00da Y: Ph\u01b0\u01a1ng ph\u00e1p T\u00e1ch chi\u1ebft hi\u1ec7u qu\u1ea3 trong x\u00e9t nghi\u1ec7m<\/strong>"},"content":{"rendered":"

T\u00e1ch chi\u1ebft nucleic acid<\/strong> l\u00e0 ph\u01b0\u01a1ng ph\u00e1p c\u01a1 b\u1ea3n trong x\u00e9t nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed. Hi\u1ec7n nay, nhi\u1ec1u ph\u01b0\u01a1ng ph\u00e1p t\u00e1ch chi\u1ebft thu nh\u1eadn nucleic acid tinh s\u1ea1ch \u0111\u00e3 \u0111\u01b0\u1ee3c ph\u00e1t tri\u1ec3n th\u00e0nh c\u00e1c b\u1ed9 kit th\u01b0\u01a1ng m\u1ea1i. Trong s\u1ed1 \u0111\u00f3, t\u00e1ch chi\u1ebft b\u1eb1ng c\u1ed9t silica l\u00e0 ph\u01b0\u01a1ng ph\u00e1p ph\u1ed5 bi\u1ebfn d\u1ef1a tr\u00ean s\u1ef1 t\u01b0\u01a1ng t\u00e1c gi\u1eefa nucleic acid v\u00e0 ch\u1ea5t h\u1ea5p th\u1ee5 r\u1eafn (th\u01b0\u1eddng l\u00e0 h\u1ea1t silica) trong c\u00e1c \u0111i\u1ec1u ki\u1ec7n c\u1ee5 th\u1ec3.<\/p>\n\n\n\n

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T\u00e1ch chi\u1ebft c\u1ed9t silica<\/strong><\/h2>\n\n\n\n

Silica, hay dioxide silic (SiO2<\/sub>), l\u00e0 m\u1ed9t d\u1ea1ng silica x\u1ed1p c\u00f3 c\u1ea5u tr\u00fac v\u00f4 \u0111\u1ecbnh h\u00ecnh. Silica bao g\u1ed3m m\u1ed9t m\u1ea1ng l\u01b0\u1edbi c\u00e1c h\u1ea1t c\u00f3 k\u00edch th\u01b0\u1edbc t\u1eeb 2-20 nm k\u1ebft n\u1ed1i v\u1edbi nhau t\u1ea1o th\u00e0nh ch\u1ea5t h\u1ea5p th\u1ee5 r\u1eafn c\u00f3 \u00e1i l\u1ef1c li\u00ean k\u1ebft m\u1ea1nh v\u00e0 \u0111\u1eb7c hi\u1ec7u v\u1edbi nucleic acid trong \u0111i\u1ec1u ki\u1ec7n ki\u1ec1m v\u00e0 mu\u1ed1i cao. Nguy\u00ean l\u00fd c\u1ee7a t\u00e1ch chi\u1ebft c\u1ed9t silica d\u1ef1a tr\u00ean \u00e1i l\u1ef1c gi\u1eefa nucleic acid t\u00edch \u0111i\u1ec7n \u00e2m v\u00e0 b\u1ec1 m\u1eb7t h\u1ea1t silica t\u00edch \u0111i\u1ec7n d\u01b0\u01a1ng trong \u0111i\u1ec1u ki\u1ec7n n\u1ed3ng \u0111\u1ed9 mu\u1ed1i chaotropic cao, gi\u00fap g\u1eafn nucleic acid l\u00ean c\u1ed9t. Trong khi \u0111\u00f3, c\u00e1c th\u00e0nh ph\u1ea7n t\u1ebf b\u00e0o v\u00e0 h\u00f3a ch\u1ea5t kh\u00e1c kh\u00f4ng b\u00e1m l\u00ean c\u1ed9t s\u1ebd b\u1ecb r\u1eeda tr\u00f4i. Cu\u1ed1i c\u00f9ng, DNA \u0111\u01b0\u1ee3c r\u1eeda gi\u1ea3i kh\u1ecfi m\u00e0ng silica b\u1eb1ng n\u01b0\u1edbc ho\u1eb7c dung d\u1ecbch \u0111\u1ec7m c\u00f3 h\u00e0m l\u01b0\u1ee3ng mu\u1ed1i th\u1ea5p. Quy tr\u00ecnh th\u1ef1c hi\u1ec7n t\u00e1ch chi\u1ebft nucleic acid b\u1eb1ng c\u1ed9t silica bao g\u1ed3m c\u00e1c b\u01b0\u1edbc: ly gi\u1ea3i t\u1ebf b\u00e0o, g\u1eafn c\u1ed9t, r\u1eeda c\u1ed9t, l\u00e0m kh\u00f4 c\u1ed9t v\u00e0 r\u1eeda gi\u1ea3i.<\/p>\n\n\n\n

\"H\u00ecnh
Figure 1. <\/strong>Ph\u01b0\u01a1ng ph\u00e1p t\u00e1ch chi\u1ebft DNA b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p c\u1ed9t silica. (a) Quy tr\u00ecnh t\u00e1ch chi\u1ebft DNA. (b) Dung d\u1ecbch \u0111\u1ec7m r\u1eeda gi\u1ea3i c\u00f2n s\u00f3t l\u1ea1i cu\u1ed1i c\u00f9ng trong m\u00e0ng silica v\u1edbi l\u1ef1c ly t\u00e2m cao (20.000\u2009g). (c) C\u00e2n b\u1eb1ng l\u1ef1c trong \u1ed1ng m\u00e0ng silica gi\u1eefa l\u1ef1c c\u0103ng b\u1ec1 m\u1eb7t c\u1ee7a \u0111\u1ec7m r\u1eeda gi\u1ea3i (Fst) v\u00e0 l\u1ef1c tr\u1ecdng tr\u01b0\u1eddng (Fg) ho\u1eb7c l\u1ef1c \u00e9p (Fp), b\u00e1n k\u00ednh mao qu\u1ea3n R, g\u00f3c gi\u1ecdt \u03b1 v\u00e0 gi\u1ecdt n\u01b0\u1edbc kh\u1ed1i l\u01b0\u1ee3ng m. (d) H\u00ecnh \u1ea3nh hi\u1ec3n vi c\u1ee7a m\u00e0ng silica.<\/em><\/figcaption><\/figure>\n\n\n\n

Trong ph\u01b0\u01a1ng ph\u00e1p t\u00e1ch c\u1ed9t silica, c\u1ed9t quay (spin-column) l\u00e0 th\u00e0nh ph\u1ea7n quan tr\u1ecdng \u0111\u1ec3 h\u1ed7 tr\u1ee3 cho qu\u00e1 tr\u00ecnh t\u00e1ch chi\u1ebft th\u1ee7 c\u00f4ng. C\u1ed9t th\u01b0\u1eddng \u0111\u01b0\u1ee3c l\u00e0m b\u1eb1ng ch\u1ea5t li\u1ec7u nh\u1ef1a \u0111\u1eb7c bi\u1ec7t (th\u01b0\u1eddng l\u00e0 polypropylene d\u00f9ng trong y t\u1ebf), c\u1ed9t ly t\u00e2m c\u00f3 k\u00edch th\u01b0\u1edbc nh\u1ecf h\u01a1n so v\u1edbi \u1ed1ng thu m\u1eabu v\u1edbi l\u1ed7 h\u1eb9p d\u01b0\u1edbi \u0111\u00e1y \u0111\u1ec3 c\u00f3 th\u1ec3 l\u1eafp v\u1eeba kh\u00edt v\u1edbi \u1ed1ng thu. Ngay ph\u00eda tr\u00ean l\u1ed7 h\u1eb9p l\u00e0 l\u1edbp m\u00e0ng silica gel c\u00f3 \u0111i\u1ec7n t\u00edch d\u01b0\u01a1ng gi\u00fap g\u1eafn v\u00e0 li\u00ean k\u1ebft nucleic acid t\u00edch \u0111i\u1ec7n \u00e2m v\u1edbi ch\u1ea5t n\u1ec1n.<\/p>\n\n\n\n

\"H\u00ecnh
Figure 2.<\/strong> C\u1ea5u t\u1ea1o c\u1ed9t v\u00e0 t\u01b0\u01a1ng t\u00e1c gi\u1eefa silica (SiO2-)<\/sup> trong c\u1ed9t spin-column<\/em><\/figcaption><\/figure>\n\n\n\n
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Nhi\u1ec1u nghi\u00ean c\u1ee9u \u0111\u00e3 ch\u1ee9ng minh c\u01a1 ch\u1ebf li\u00ean k\u1ebft c\u1ee7a axit nucleic tr\u00ean b\u1ec1 m\u1eb7t silica ch\u1ecbu s\u1ef1 t\u00e1c \u0111\u1ed9ng c\u1ee7a  b\u1ed1n y\u1ebfu t\u1ed1 ch\u00ednh g\u1ed3m: li\u00ean k\u1ebft hydro, c\u1ea7u mu\u1ed1i, l\u1ef1c t\u0129nh \u0111i\u1ec7n h\u00ecnh th\u00e0nh gi\u1eefa axit nucleic v\u00e0 b\u1ec1 m\u1eb7t silica v\u00e0 \u0111\u1ed9 h\u00f2a tan c\u1ee7a axit nucleic. Do \u0111\u00f3, li\u00ean k\u1ebft axit nucleic \u0111\u01b0\u1ee3c t\u1ed1i \u01b0u h\u00f3a v\u1edbi c\u00e1c dung d\u1ecbch \u0111\u1ec7m \u0111\u1eb7c tr\u01b0ng \u1edf n\u1ed3ng \u0111\u1ed9 mu\u1ed1i v\u00e0 pH ch\u00ednh x\u00e1c v\u1edbi lo\u1ea1i m\u1eabu c\u1ea7n t\u00e1ch chi\u1ebft.<\/p>\n\n\n\n

M\u1ed7i lo\u1ea1i t\u1ebf b\u00e0o m\u1ee5c ti\u00eau th\u01b0\u1eddng \u0111\u01b0\u1ee3c \u0111\u1eb7c tr\u01b0ng b\u1edfi c\u1ea5u t\u1ea1o l\u1edbp m\u00e0ng v\u00e0 th\u00e0nh ph\u1ea7n v\u00e1ch t\u1ebf b\u00e0o kh\u00e1c nhau n\u00ean s\u1ebd ph\u00f9 h\u1ee3p v\u1edbi c\u00e1c lo\u1ea1i h\u00f3a ch\u1ea5t ly gi\u1ea3i kh\u00e1c nhau. V\u00ed d\u1ee5, \u0111\u1ec3 t\u00e1ch chi\u1ebft m\u1eabu m\u00f4, sinh thi\u1ebft v\u00e0 d\u1ecbch ph\u1ebft y t\u1ebf ch\u1ea9n \u0111o\u00e1n ung th\u01b0 c\u1ed5 t\u1eed cung (HPV), ABT s\u1eed d\u1ee5ng dung d\u1ecbch \u0111\u1ec7m \u0111\u1eb7c tr\u01b0ng l\u00e0 TL buffer v\u00e0 CL buffer. Tr\u01b0\u1eddng h\u1ee3p ch\u1ea9n \u0111o\u00e1n Vi\u00eam gan B v\u00e0 Vi\u00eam gan C , ABT s\u1eed d\u1ee5ng dung d\u1ecbch \u0111\u1ec7m NL buffer v\u00e0 RL buffer t\u00e1ch chi\u1ebft m\u1eabu huy\u1ebft thanh\/huy\u1ebft t\u01b0\u01a1ng. Nh\u01b0 v\u1eady, m\u1ed7i lo\u1ea1i n\u1ec1n m\u1eabu c\u00f3 th\u1ec3 t\u1ed1i \u01b0u v\u1edbi lo\u1ea1i buffer ly gi\u1ea3i  v\u00e0 quy tr\u00ecnh x\u1eed l\u00fd kh\u00e1c nhau. \u0110\u1ed1i v\u1edbi c\u00e1c lo\u1ea1i n\u1ec1n m\u1eabu m\u1edbi, ng\u01b0\u1eddi s\u1eed d\u1ee5ng c\u1ea7n \u0111\u00e1nh gi\u00e1 \u0111\u1ec3 l\u1ef1a ch\u1ecdn b\u1ed9 kit ly tr\u00edch hi\u1ec7u qu\u1ea3 nh\u1ea5t cho lo\u1ea1i n\u1ec1n m\u1eabu m\u00e0 m\u00ecnh c\u1ea7n ly tr\u00edch.<\/p>\n\n\n\n

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C\u00e1c b\u01b0\u1edbc th\u1ef1c hi\u1ec7n t\u00e1ch chi\u1ebft c\u1ed9t silica c\u1ee7a ABT tr\u00ean n\u1ec1n m\u1eabu th\u00fa y<\/strong><\/h2>\n\n\n\n

Kit t\u00e1ch c\u1ed9t silica do ABT s\u1ea3n xu\u1ea5t \u1ee9ng d\u1ee5ng trong x\u00e9t nghi\u1ec7m ch\u1ea9n \u0111o\u00e1n b\u1ec7nh trong \u0111a d\u1ea1ng l\u0129nh v\u1ef1c kh\u00e1c nhau. Chi ti\u1ebft c\u00e1c b\u01b0\u1edbc th\u1ef1c hi\u1ec7n t\u00e1ch chi\u1ebft c\u1ed9t silica tr\u00ean n\u1ec1n m\u1eabu th\u00fa y nh\u1eb1m ch\u1ea9n \u0111o\u00e1n m\u1ed9t s\u1ed1 b\u1ec7nh tr\u00ean heo, ch\u00f3, m\u00e8o\u2026 \u0111\u01b0\u1ee3c tr\u00ecnh b\u00e0y nh\u01b0 \u1edf quy tr\u00ecnh sau:<\/p>\n\n\n\n

\"H\u00ecnh<\/a>
Figure 3.<\/em><\/strong> Quy tr\u00ecnh th\u1ef1c hi\u1ec7n t\u00e1ch chi\u1ebft c\u1ed9t silica s\u1eed d\u1ee5ng b\u1ed9 kit TopPURE\u00ae VIRAL DNA\/RNA EXTRACTION KIT (HI-172)<\/em><\/figcaption><\/figure>\n\n\n\n

Ly gi\u1ea3i t\u1ebf b\u00e0o:<\/strong> M\u1eabu th\u00fa y bao g\u1ed3m m\u1eabu t\u1ebf b\u00e0o nu\u00f4i c\u1ea5y, vi khu\u1ea9n, huy\u1ec5n d\u1ecbch, m\u1eabu qu\u00e9t b\u1ec1 m\u1eb7t, m\u1eabu d\u1ecbch ph\u1ebft (y t\u1ebf), d\u1ecbch ph\u1ebft (h\u1ecdng\/m\u0169i), huy\u1ebft thanh, huy\u1ebft t\u01b0\u01a1ng v\u00e0 m\u1eabu m\u00e1u to\u00e0n ph\u1ea7n t\u1eeb ng\u01b0\u1eddi v\u00e0 \u0111\u1ed9ng v\u1eadt c\u00f3 v\u00fa \u0111\u01b0\u1ee3c ly gi\u1ea3i nh\u1edd dung d\u1ecbch \u0111\u1ec7m VBL Buffer. Trong dung d\u1ecbch \u0111\u1ec7m ly gi\u1ea3i th\u01b0\u1eddng ch\u1ee9a c\u00e1c ch\u1ea5t c\u00f3 kh\u1ea3 n\u0103ng ly gi\u1ea3i m\u1eabu \u00a0nh\u01b0 Triton X100, SDS, Tween 20 v\u00e0 mu\u1ed1i chaotropic n\u1ed3ng \u0111\u1ed9 cao gi\u00fap h\u1ed7 tr\u1ee3 h\u00f2a tan protein. Sau \u0111\u00f3 b\u1ed5 sung\u00a0 proteinase K v\u00e0 \u1ee7 \u1edf 72\u00b0C nh\u1eb1m h\u1ed7 tr\u1ee3 ph\u00e1 v\u1ee1 c\u00e1c protein m\u00e0ng, gi\u1ea3i ph\u00f3ng nucleic acid ra kh\u1ecfi t\u1ebf b\u00e0o v\u00e0 m\u00f4.<\/p>\n\n\n\n

G\u1eafn c\u1ed9t:<\/strong> H\u1ed7n h\u1ee3p sau ly gi\u1ea3i s\u1ebd \u0111\u01b0\u1ee3c chuy\u1ec3n l\u00ean c\u1ed9t silica. L\u00fac n\u00e0y, mu\u1ed1i chaotropic n\u1ed3ng \u0111\u1ed9 cao cung c\u1ea5p \u0111i\u1ec7n t\u00edch d\u01b0\u01a1ng l\u00e0m c\u1ea7u n\u1ed1i \u0111\u1ec3 DNA b\u00e1m l\u00ean m\u00e0ng silica, ethanol c\u1ea1nh tranh n\u01b0\u1edbc v\u1edbi nucleic acid gi\u00fap h\u1ed7 tr\u1ee3 cho vi\u1ec7c g\u1eafn acid nucleic l\u00ean m\u00e0ng silica. C\u00e1c th\u00e0nh ph\u1ea7n t\u1ea1p nhi\u1ec5m kh\u00e1c \u0111\u01b0\u1ee3c lo\u1ea1i b\u1ecf th\u00f4ng qua b\u01b0\u1edbc ly t\u00e2m. Trong qu\u00e1 tr\u00ecnh n\u00e0y, ethanol n\u1ed3ng \u0111\u1ed9 cao th\u01b0\u1eddng \u0111\u01b0\u1ee3c b\u1ed5 sung gi\u00fap t\u0103ng c\u01b0\u1eddng qu\u00e1 tr\u00ecnh g\u1eafn nucleic acid l\u00ean c\u1ed9t do \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn s\u1ef1 li\u00ean k\u1ebft c\u1ee7a nucleic acid v\u1edbi silica. <\/p>\n\n\n\n

* Note: <\/strong>Ethanol \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn kh\u1ea3 n\u0103ng li\u00ean k\u1ebft v\u1edbi nucleic acid. Do \u0111\u00f3, l\u01b0\u1ee3ng ethanol b\u1ed5 sung \u0111\u01b0\u1ee3c ABT t\u1ed1i \u01b0u h\u00f3a cho t\u1eebng b\u1ed9 kit t\u00e1ch chi\u1ebft s\u1eed d\u1ee5ng. N\u1ebfu s\u1eed d\u1ee5ng qu\u00e1 nhi\u1ec1u ethanol s\u1ebd \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn ch\u1ea5t l\u01b0\u1ee3ng axit nucleic thu nh\u1eadn d\u1eabn \u0111\u1ebfn DNA\/RNA d\u1ec5 b\u1ecb ph\u00e2n h\u1ee7y \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn k\u1ebft qu\u1ea3 A260\/A280 v\u00e0 hi\u1ec7u su\u1ea5t thu h\u1ed3i. Qu\u00e1 \u00edt Ethanol s\u1ebd kh\u00f3 r\u1eeda s\u1ea1ch h\u1ebft mu\u1ed1i kh\u1ecfi m\u00e0ng.<\/em><\/p>\n\n\n\n

Lo\u1ea1i b\u1ecf t\u1ea1p ch\u1ea5t<\/strong>:<\/strong> Dung d\u1ecbch WB1 ch\u1ee9a mu\u1ed1i chaotropic n\u1ed3ng \u0111\u1ed9 th\u1ea5p \u0111\u1ec3 r\u1eeda protein v\u00e0 s\u1eafc t\u1ed1. Dung d\u1ecbch WB2  ch\u1ee9a ethanol n\u1ed3ng \u0111\u1ed9 cao \u0111\u1ec3 lo\u1ea1i mu\u1ed1i chaotropic v\u00e0 c\u00e1c th\u00e0nh ph\u1ea7n c\u00f2n s\u00f3t l\u1ea1i. Vi\u1ec7c lo\u1ea1i b\u1ecf mu\u1ed1i chaotropic l\u00e0 r\u1ea5t quan tr\u1ecdng gi\u00fap n\u00e2ng cao hi\u1ec7u su\u1ea5t thu h\u1ed3i v\u00e0 ch\u1ea5t l\u01b0\u1ee3ng nucleic acid.<\/p>\n\n\n\n

Thu nh\u1eadn nucleic acid:<\/strong> Sau b\u01b0\u1edbc r\u1eeda hai l\u1ea7n, h\u1ea7u h\u1ebft c\u00e1c quy tr\u00ecnh \u0111\u1ec1u c\u00f3 m\u1ed9t b\u01b0\u1edbc ly t\u00e2m \u0111\u1ec3 l\u00e0m l\u00e0m kh\u00f4 c\u1ed9t. Vi\u1ec7c n\u00e0y gi\u00fap lo\u1ea1i b\u1ecf ethanol v\u00e0 l\u00e0 c\u1ea7n thi\u1ebft \u0111\u1ec3 c\u00f3 dung d\u1ecbch r\u1eeda gi\u1ea3i s\u1ea1ch. Sau\u00a0 \u0111\u00f3, dung d\u1ecbch ly gi\u1ea3i EB (n\u01b0\u1edbc x\u1eed l\u00fd DEPC cho RNA ho\u1eb7c dung d\u1ecbch TE c\u00f3 pH>9) \u00a0gi\u00fap gi\u1ea3i ph\u00f3ng c\u00e1c DNA\/RNA s\u1ea1ch kh\u1ecfi m\u00e0ng silica. Nucleic acid tinh s\u1ea1ch \u0111\u01b0\u1ee3c b\u1ea3o qu\u1ea3n \u1edf -20\u00b0C ph\u1ee5c v\u1ee5 cho c\u00e1c th\u00ed nghi\u1ec7m ti\u1ebfp theo.<\/p>\n\n\n\n

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C\u00e1c l\u01b0u \u00fd an to\u00e0n ph\u00f2ng th\u00ed nghi\u1ec7m<\/strong><\/h2>\n\n\n\n

T\u00e1ch chi\u1ebft silica l\u00e0 m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p quan tr\u1ecdng trong vi\u1ec7c thu nh\u1eadn nucleic acid tinh s\u1ea1ch. Ph\u01b0\u01a1ng ph\u00e1p n\u00e0y c\u00f3 \u01b0u \u0111i\u1ec3m t\u00e1ch chi\u1ebft nhanh ch\u00f3ng, \u0111\u01a1n gi\u1ea3n thu nh\u1eadn nucleic acid tinh s\u1ea1ch cao. Ngo\u00e0i ra, b\u1ed9 kit c\u00f3 \u01b0u \u0111i\u1ec3m kh\u00f4ng s\u1eed d\u1ee5ng h\u00f3a ch\u1ea5t \u0111\u1ed9c h\u1ea1i nh\u01b0 phenol, chloroform. Tuy nhi\u00ean \u0111\u1ec3 s\u1eed d\u1ee5ng b\u1ed9 kit ng\u01b0\u1eddi s\u1eed d\u1ee5ng c\u1ea7n trang b\u1ecb c\u00e1c thi\u1ebft b\u1ecb h\u1ed7 tr\u1ee3 nh\u01b0 m\u00e1y ly t\u00e2m, vortex v\u00e0 tu\u00e2n th\u1ee7 \u0111\u00fang quy tr\u00ecnh \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o k\u1ebft qu\u1ea3 t\u00e1ch chi\u1ebft ch\u00ednh x\u00e1c:<\/p>\n\n\n\n

C\u00e1c m\u1eabu x\u00e9t nghi\u1ec7m ph\u1ea3i \u0111\u01b0\u1ee3c x\u1eed l\u00fd theo \u0111\u00fang quy tr\u00ecnh \u0111\u1ec1 ph\u00f2ng ph\u00e1t t\u00e1n vi khu\u1ea9n\/virus ra ngo\u00e0i m\u00f4i tr\u01b0\u1eddng. Kit ph\u1ea3i \u0111\u01b0\u1ee3c thao t\u00e1c trong t\u1ee7 thao t\u00e1c PCR ho\u1eb7c t\u1ee7 ATSH v\u00e0 lu\u00f4n lu\u00f4n m\u1eb7c \u00e1o Blouse, s\u1eed d\u1ee5ng g\u0103ng tay, kh\u1ea9u trang.<\/p>\n\n\n\n

Ngo\u00e0i ra, c\u1ea7n s\u1eed d\u1ee5ng h\u00f3a ch\u1ea5t \u0111\u00fang c\u00e1ch, tr\u00e1nh ti\u1ebfp x\u00fac tr\u1ef1c ti\u1ebfp, v\u00e0 l\u00e0m vi\u1ec7c trong khu v\u1ef1c th\u00f4ng gi\u00f3 t\u1ed1t. Khi ph\u00e1t hi\u1ec7n s\u1ef1 c\u1ed1 nh\u01b0 r\u00f2 r\u1ec9 h\u00f3a ch\u1ea5t, c\u1ea7n x\u1eed l\u00fd ngay theo quy tr\u00ecnh an to\u00e0n c\u1ee7a ph\u00f2ng th\u00ed nghi\u1ec7m.<\/p>\n\n\n\n

<\/div>\n\n\n\n

T\u00e0i li\u1ec7u tham kh\u1ea3o<\/strong><\/h2>\n\n\n\n
    \n
  1. Ali, Nasir; Rampazzo, Rita de C\u00e1ssia Pontello; Costa, Alexandre Dias Tavares; Krieger, Marco Aurelio (2017). Current Nucleic Acid Extraction Methods and Their Implications to Point-of-Care Diagnostics. BioMed Research International, 2017(), 1\u201313. <\/em>doi:10.1155\/2017\/9306564<\/li>\n\n\n\n
  2. Hu, WP., Chen, YC. & Chen, WY. Improve sample preparation process for miRNA isolation from the culture cells by using silica fiber membrane. Sci Rep<\/em> 10<\/strong>, 21132 (2020). https:\/\/doi.org\/10.1038\/s41598-020-78202-8<\/a><\/li>\n\n\n\n
  3. Sahu, O., & Singh, N.K. (2019). Significance of bioadsorption process on textile industry wastewater. The Impact and Prospects of Green Chemistry for Textile Technology<\/em>. Lee, H., Na, W., Park, C. et al.<\/em> Centrifugation-free extraction of circulating nucleic acids using immiscible liquid under vacuum pressure. Sci Rep<\/em> 8, 5467 (2018). https:\/\/doi.org\/10.1038\/s41598-018-23766-9<\/a><\/li>\n<\/ol>\n\n\n\n

    <\/p>","protected":false},"excerpt":{"rendered":"

    T\u00e1ch chi\u1ebft nucleic acid l\u00e0 ph\u01b0\u01a1ng ph\u00e1p c\u01a1 b\u1ea3n trong x\u00e9t nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed. Hi\u1ec7n nay, nhi\u1ec1u ph\u01b0\u01a1ng ph\u00e1p t\u00e1ch chi\u1ebft thu nh\u1eadn nucleic acid tinh s\u1ea1ch \u0111\u00e3 \u0111\u01b0\u1ee3c ph\u00e1t tri\u1ec3n th\u00e0nh c\u00e1c b\u1ed9 kit th\u01b0\u01a1ng m\u1ea1i. Trong s\u1ed1 \u0111\u00f3, t\u00e1ch chi\u1ebft b\u1eb1ng c\u1ed9t silica l\u00e0 ph\u01b0\u01a1ng ph\u00e1p ph\u1ed5 bi\u1ebfn d\u1ef1a tr\u00ean s\u1ef1 […]<\/p>","protected":false},"author":1,"featured_media":12564,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/admin\/"},"uagb_comment_info":0,"uagb_excerpt":"T\u00e1ch chi\u1ebft nucleic acid l\u00e0 ph\u01b0\u01a1ng ph\u00e1p c\u01a1 b\u1ea3n trong x\u00e9t nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed. Hi\u1ec7n nay, nhi\u1ec1u ph\u01b0\u01a1ng ph\u00e1p t\u00e1ch chi\u1ebft thu nh\u1eadn nucleic acid tinh s\u1ea1ch \u0111\u00e3 \u0111\u01b0\u1ee3c ph\u00e1t tri\u1ec3n th\u00e0nh c\u00e1c b\u1ed9 kit th\u01b0\u01a1ng m\u1ea1i. Trong s\u1ed1 \u0111\u00f3, t\u00e1ch chi\u1ebft b\u1eb1ng c\u1ed9t silica l\u00e0 ph\u01b0\u01a1ng ph\u00e1p ph\u1ed5 bi\u1ebfn d\u1ef1a tr\u00ean s\u1ef1…","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/11572","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=11572"}],"version-history":[{"count":8,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/11572\/revisions"}],"predecessor-version":[{"id":13067,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/11572\/revisions\/13067"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/12564"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=11572"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/categories?post=11572"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/tags?post=11572"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}