{"id":11346,"date":"2024-06-21T17:09:24","date_gmt":"2024-06-21T09:39:24","guid":{"rendered":"https:\/\/abtvn.com\/?p=11346"},"modified":"2025-08-06T13:45:51","modified_gmt":"2025-08-06T06:15:51","slug":"vi-sao-lua-chon-taqman-probe-cho-real-time-pcr","status":"publish","type":"post","link":"https:\/\/abtvn.com\/en\/vi-sao-lua-chon-taqman-probe-cho-real-time-pcr\/","title":{"rendered":"V\u00ec sao l\u1ef1a ch\u1ecdn Taqman probe cho Real-time PCR?<\/strong>"},"content":{"rendered":"

Real-time PCR l\u00e0 g\u00ec?<\/strong><\/h2>\n\n\n\n

Real-time PCR<\/a>,<\/strong> hay c\u00f2n g\u1ecdi l\u00e0 PCR \u0111\u1ecbnh l\u01b0\u1ee3ng theo th\u1eddi gian th\u1ef1c (qPCR), \u0111\u01b0\u1ee3c ph\u00e1t tri\u1ec3n v\u00e0o n\u0103m 1993 \u0111\u1ec3 ph\u00e1t hi\u1ec7n v\u00e0 \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic. C\u00f4ng ngh\u1ec7 n\u00e0y \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng r\u1ed9ng r\u00e3i trong h\u1ea7u h\u1ebft c\u00e1c l\u0129nh v\u1ef1c nghi\u00ean c\u1ee9u y sinh, n\u00f4ng nghi\u1ec7p, th\u1ef1c ph\u1ea9m v\u00e0 khoa h\u1ecdc m\u00f4i tr\u01b0\u1eddng. M\u1eb7c d\u00f9 c\u00f3 nhi\u1ec1u ph\u01b0\u01a1ng ph\u00e1p ch\u1ea9n \u0111o\u00e1n m\u1edbi \u0111\u01b0\u1ee3c ph\u00e1t tri\u1ec3n trong nh\u1eefng n\u0103m g\u1ea7n \u0111\u00e2y nh\u01b0 ph\u01b0\u01a1ng ph\u00e1p khu\u1ebfch \u0111\u1ea1i \u0111\u1eb3ng nhi\u1ec7t, ph\u01b0\u01a1ng ph\u00e1p \u0111\u1eb3ng nhi\u1ec7t trung gian v\u00f2ng l\u1eb7p (LAMP), khu\u1ebfch \u0111\u1ea1i v\u00f2ng tr\u00f2n l\u0103n (RCA), khu\u1ebfch \u0111\u1ea1i axit nucleic \u0111\u1eb3ng nhi\u1ec7t b\u1eb1ng Recombinase Polymerase Amplification (RPA) v\u00e0 k\u1ef9 thu\u1eadt ch\u1ea9n \u0111o\u00e1n d\u1ef1a tr\u00ean CRISPR c\u00f3 \u0111\u1ed9 nh\u1ea1y cao (SHERLOCK). qPCR v\u1eabn \u0111\u01b0\u1ee3c coi l\u00e0 c\u00f4ng ngh\u1ec7 m\u1ea1nh m\u1ebd nh\u1ea5t trong x\u00e9t nghi\u1ec7m ch\u1ea9n \u0111o\u00e1n l\u00e2m s\u00e0ng, \u0111\u1eb7c bi\u1ec7t \u0111\u1ed1i v\u1edbi c\u00e1c b\u1ec7nh truy\u1ec1n nhi\u1ec5m.<\/p>\n\n\n\n

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V\u1ec1 m\u1eb7t \u0111\u1ecbnh ngh\u0129a, k\u1ef9 thu\u1eadt Real-time PCR<\/a><\/strong> \u0111\u01b0\u1ee3c ph\u00e1t tri\u1ec3n d\u1ef1a tr\u00ean k\u1ef9 thu\u1eadt PCR. Real-time PCR s\u1eed d\u1ee5ng c\u00e1c ph\u00e2n t\u1eed ph\u00e1t hu\u1ef3nh quang c\u00f3 kh\u1ea3 n\u0103ng li\u00ean k\u1ebft v\u1edbi gene m\u1ee5c ti\u00eau, t\u1eeb \u0111\u00f3 cho ph\u00e9p theo d\u00f5i tr\u1ef1c ti\u1ebfp\u00a0 s\u1ed1 l\u01b0\u1ee3ng b\u1ea3n sao DNA m\u1ee5c ti\u00eau t\u1ea1o ra ngay sau m\u1ed7i chu k\u1ef3 nhi\u1ec7t c\u1ee7a ph\u1ea3n \u1ee9ng b\u1eb1ng c\u00f4ng ngh\u1ec7 hu\u1ef3nh quang. Nh\u01b0 v\u1eady, c\u01b0\u1eddng \u0111\u1ed9 t\u00edn hi\u1ec7u hu\u1ef3nh quang m\u00e1y ghi nh\u1eadn s\u1ebd ph\u1ea3n \u00e1nh tr\u1ef1c ti\u1ebfp l\u01b0\u1ee3ng DNA khu\u1ebfch \u0111\u1ea1i gi\u00fap ng\u01b0\u1eddi l\u00e0m th\u00ed nghi\u1ec7m c\u00f3 th\u1ec3 quan s\u00e1t t\u1ea1i c\u00e1c th\u1eddi \u0111i\u1ec3m c\u1ee5 th\u1ec3.<\/p>\n\n\n\n

\"H\u00ecnh
Figure 1.<\/em><\/strong> Quy tr\u00ecnh Real-time PCR. Th\u00f4ng tin chi ti\u1ebft tham kh\u1ea3o b\u00e0i vi\u1ebft https:\/\/abtvn.com\/real-time-pcr\/<\/em><\/figcaption><\/figure>\n\n\n\n

D\u1ef1a tr\u00ean ch\u1ea5t ph\u00e1t hu\u1ef3nh quang s\u1eed d\u1ee5ng v\u00e0 t\u00ednh \u0111\u1eb7c hi\u1ec7u c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p ph\u00e1t hi\u1ec7n, Real-time PCR c\u00f3 th\u1ec3 s\u1eed d\u1ee5ng (1) c\u00e1c ph\u00e2n t\u1eed hu\u1ef3nh quang g\u1eafn ch\u00e8n v\u00e0o DNA s\u1ee3i \u0111\u00f4i (SYBR Green ho\u1eb7c SYTO9) v\u00e0 (2) c\u00e1c ph\u00e2n t\u1eed \u0111\u1ea7u d\u00f2 \u0111\u00e1nh d\u1ea5u hu\u1ef3nh quang g\u1ed3m \u0111\u1ea7u d\u00f2 th\u1ee7y ph\u00e2n (th\u01b0\u1eddng l\u00e0 TaqMan probes) ho\u1eb7c \u0111\u1ea7u d\u00f2 lai v\u00e0 ph\u00e2n t\u1eed beacons. Trong \u0111\u00f3, c\u00e1c \u0111\u1ea7u d\u00f2 TaqMan \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng r\u1ed9ng r\u00e3i \u0111\u1ec3 ph\u00e1t hi\u1ec7n v\u00e0 \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic v\u00e0 th\u01b0\u1eddng \u0111\u01b0\u1ee3c ph\u00e1t tri\u1ec3n th\u00e0nh b\u1ed9 kit th\u01b0\u01a1ng m\u1ea1i \u0111\u1ec3 ch\u1ea9n \u0111o\u00e1n l\u00e2m s\u00e0ng.<\/p>\n\n\n\n

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C\u01a1 ch\u1ebf hu\u1ef3nh quang c\u1ee7a \u0111\u1ea7u d\u00f2 Taqman (Taqman Probe)<\/strong><\/h2>\n\n\n\n

\u0110\u1ea7u d\u00f2 TaqMan<\/a><\/strong> l\u00e0 m\u1ed9t lo\u1ea1i \u0111\u1ea7u d\u00f2 \u0111\u00e1nh d\u1ea5u hu\u1ef3nh quang ph\u1ed5 bi\u1ebfn s\u1eed d\u1ee5ng trong Real-time PCR \u0111\u1ec3 ph\u00e1t hi\u1ec7n v\u00e0 \u0111\u1ecbnh l\u01b0\u1ee3ng m\u1ed9t ho\u1eb7c nhi\u1ec1u t\u00e1c nh\u00e2n m\u1ee5c ti\u00eau c\u00f3 trong m\u1eabu.<\/p>\n\n\n\n

C\u1ea5u t\u1ea1o c\u1ee7a \u0111\u1ea7u d\u00f2 Taqman l\u00e0 \u0111o\u1ea1n oligonucleotide ng\u1eafn kho\u1ea3ng 25-30 nu c\u00f3 kh\u1ea3 n\u0103ng li\u00ean k\u1ebft b\u1ed5 sung v\u1edbi gene m\u1ee5c ti\u00eau \u1edf gi\u1eefa 2 m\u1ed3i xu\u00f4i v\u00e0 ng\u01b0\u1ee3c. \u0110\u1ea7u 5\u2019 c\u1ee7a \u0111\u1ea7u d\u00f2 \u0111\u01b0\u1ee3c \u0111\u00e1nh d\u1ea5u\u00a0b\u1eb1ng m\u1ed9t ch\u1ea5t ph\u00e1t hu\u1ef3nh quang (donor hay reporter) v\u00e0 \u0111\u1ea7u 3\u2019 \u0111\u01b0\u1ee3c \u0111\u00e1nh d\u1ea5u b\u1eb1ng m\u1ed9t ch\u1ea5t h\u1ea5p th\u1ee5 (acceptor hay quencher). N\u1ebfu m\u1ed9t ch\u1ea5t ph\u00e1t hu\u1ef3nh quang h\u1ea5p th\u1ee5 n\u0103ng l\u01b0\u1ee3ng \u00e1nh s\u00e1ng \u1edf b\u01b0\u1edbc s\u00f3ng k\u00edch th\u00edch (H\u00ecnh 2)<\/em><\/strong>. Theo nguy\u00ean t\u1eafc truy\u1ec1n n\u0103ng l\u01b0\u1ee3ng c\u1ed9ng h\u01b0\u1edfng hu\u1ef3nh quang (FRET), ch\u1ea5t ph\u00e1t hu\u1ef3nh quang v\u00e0 ch\u1ea5t h\u1ea5p th\u1ee5 n\u1eb1m g\u1ea7n nhau v\u00e0 n\u0103ng l\u01b0\u1ee3ng k\u00edch th\u00edch t\u1eeb ch\u1ea5t ph\u00e1t hu\u1ef3nh quang s\u1ebd b\u1ecb d\u1eadp t\u1eaft b\u1ecb b\u1edfi ch\u1ea5t h\u1ea5p th\u1ee5, do \u0111\u00f3 kh\u00f4ng c\u00f3 t\u00edn hi\u1ec7u hu\u1ef3nh quang ph\u00e1t ra.<\/p>\n\n\n\n

\"H\u00ecnh
Figure 2.<\/strong> M\u00f4 h\u00ecnh ho\u1ea1t \u0111\u1ed9ng c\u1ee7a \u0111\u1ea7u d\u00f2 Taqman<\/em><\/figcaption><\/figure>\n\n\n\n

Khi c\u00f3 s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a tr\u00ecnh t\u1ef1 gene m\u1ee5c ti\u00eau, enzyme polymerase k\u00e9o d\u00e0i m\u1ed3i tr\u00ean m\u1ea1ch khu\u00f4n, b\u1ed5 sung nucleotide t\u1ea1o m\u1ed9t chu\u1ed7i DNA b\u1ed5 sung. Khi t\u1edbi v\u1ecb tr\u00ed \u0111\u1ea7u d\u00f2, polymerase kh\u00f4ng th\u1ec3 k\u00e9o d\u00e0i \u0111\u1ea7u d\u00f2 v\u00ec n\u00f3 kh\u00f4ng ch\u1ee9a nh\u00f3m OH t\u1ef1 do. L\u00fac n\u00e0y Taqman s\u1ebd b\u1ecb th\u1ee7y ph\u00e2n \u00a0b\u1edfi ho\u1ea1t t\u00ednh 5\u2019-3\u2019 exonuclease c\u1ee7a Polymerase trong ph\u1ea3n \u1ee9ng lu\u00e2n nhi\u1ec7t. Ch\u1ea5t ph\u00e1t hu\u1ef3nh quang \u1edf \u0111\u1ea7u 5\u2019 \u0111\u01b0\u1ee3c gi\u1ea3i ph\u00f3ng kh\u1ecfi ch\u1ea5t h\u1ea5p th\u1ee5, ch\u1ea5t h\u1ea5p th\u1ee5 kh\u00f4ng c\u00f2n kh\u1ea3 n\u0103ng h\u1ea5p th\u1ee5 t\u00edn hi\u1ec7u hu\u1ef3nh quang ph\u00e1t ra do \u0111\u00f3 s\u1ebd ph\u00e1t t\u00edn hi\u1ec7u hu\u1ef3nh quang khi nh\u1eadn \u0111\u01b0\u1ee3c \u00e1nh s\u00e1ng k\u00edch th\u00edch. H\u1ec7 th\u1ed1ng ghi nh\u1eadn c\u01b0\u1eddng \u0111\u1ed9 hu\u1ef3nh quang t\u0103ng sau m\u1ed7i chu k\u1ef3 t\u1ef7 l\u1ec7 thu\u1eadn v\u1edbi l\u01b0\u1ee3ng DNA khu\u1ebfch \u0111\u1ea1i \u0111\u01b0\u1ee3c t\u1ea1o ra.<\/p>\n\n\n\n

Qu\u00e1 tr\u00ecnh th\u1ee7y ph\u00e2n \u0111\u1ea7u d\u00f2 c\u0169ng gi\u00fap lo\u1ea1i b\u1ecf \u0111\u1ea7u d\u00f2 kh\u1ecfi gene m\u1ee5c ti\u00eau, cho ph\u00e9p ti\u1ebfp t\u1ee5c k\u00e9o d\u00e0i m\u1ed3i  \u0111\u1ec3 t\u1ed5ng h\u1ee3p \u0111\u1ebfn cu\u1ed1i s\u1ee3i DNA khu\u00f4n. Do \u0111\u00f3, vi\u1ec7c \u0111\u01b0a \u0111\u1ea7u d\u00f2 v\u00e0o kh\u00f4ng g\u00e2y c\u1ea3n tr\u1edf qu\u00e1 tr\u00ecnh khu\u1ebfch \u0111\u1ea1i.<\/p>\n\n\n\n

Trong th\u1ef1c t\u1ebf, kh\u1ea3 n\u0103ng d\u1eadp t\u1eaft t\u00edn hi\u1ec7u c\u1ee7a ch\u1ea5t h\u1ea5p th\u1ee5 kh\u00f4ng ho\u00e0n to\u00e0n v\u00e0 ph\u1ee5 thu\u1ed9c v\u00e0o nhi\u1ec1u y\u1ebfu t\u1ed1 kh\u00e1c nhau. H\u01a1n n\u1eefa, s\u1ed1 l\u01b0\u1ee3ng DNA \u0111\u00edch nh\u00e2n l\u00ean trong giai \u0111o\u1ea1n \u0111\u1ea7u ch\u01b0a \u0111\u1ee7 \u0111\u1ec3 ch\u1ea5t ph\u00e1t hu\u1ef3nh quang nh\u1eadn \u0111\u01b0\u1ee3c \u00e1nh s\u00e1ng k\u00edch th\u00edch v\u00e0 ph\u00e1t ra \u00e1nh s\u00e1ng hu\u1ef3nh quang \u0111\u1ec3 m\u00e1y thu nh\u1eadn. Do \u0111\u00f3, giai \u0111o\u1ea1n \u1ee7 th\u01b0\u1eddng t\u1ea1o ra t\u00edn hi\u1ec7u n\u1ec1n (baseline) trong ph\u1ea3n \u1ee9ng Real-time PCR s\u1eed d\u1ee5ng \u0111\u1ea7u d\u00f2 Taqman.<\/p>\n\n\n\n

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B\u1ea3ng 1. <\/strong>Th\u00f4ng tin v\u1ec1 ch\u1ea5t ph\u00e1t hu\u1ef3nh quang \u2013 ch\u1ea5t h\u1ea5p th\u1ee5 ph\u00f9 h\u1ee3p v\u1edbi b\u01b0\u1edbc s\u00f3ng c\u1ee5 th\u1ec3.<\/strong><\/em><\/p>\n\n\n\n

\"B\u1ea3ng<\/figure>\n\n\n\n

\u0110\u1ec3 ch\u1ecdn l\u1ef1a hu\u1ef3nh quang cho \u0111\u1ea7u d\u00f2 Taqman, c\u00e1c ch\u1ea5t ph\u00e1t hu\u1ef3nh quang (Reporter) \u0111\u01b0\u1ee3c l\u1ef1a ch\u1ecdn ph\u1ee5 thu\u1ed9c v\u00e0o \u0111\u1ed9 d\u00e0i s\u00f3ng c\u1ee7a ngu\u1ed3n s\u00e1ng k\u00edch th\u00edch, k\u00eanh m\u00e0u tr\u00ean thi\u1ebft b\u1ecb Real-time \u0111ang s\u1eed d\u1ee5ng v\u00e0 c\u00e1c ch\u1ea5t h\u1ea5p th\u1ee5 t\u01b0\u01a1ng \u1ee9ng. C\u00e1c ch\u1ea5t h\u1ea5p th\u1ee5 (Quencher) trong \u0111\u1ea7u d\u00f2 Taqman g\u1ed3m 2 lo\u1ea1i ch\u1ea5t h\u1ea5p th\u1ee5 ph\u00e1t hu\u1ef3nh quang (TAMRA) v\u00e0 ch\u1ea5t h\u1ea5p th\u1ee5 ph\u00e1t nhi\u1ec7t (DABCYL,\u00a0 BHQ1, BHQ2, BBQ). Trong \u0111\u00f3, nh\u00f3m ch\u1ea5t h\u1ea5p th\u1ee5 ph\u00e1t nhi\u1ec7t th\u01b0\u1eddng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng v\u00ec kh\u1ea3 n\u0103ng h\u1ea5p th\u1ee5 hu\u1ef3nh quang r\u1ea5t m\u1ea1nh kh\u00f4ng cho b\u1ea5t c\u1ee9 hu\u1ef3nh quang n\u00e0o ph\u00e1t ra \u0111\u01b0\u1ee3c t\u1eeb ch\u1ea5t ph\u00e1t hu\u1ef3nh quang. \u0110\u1ed3ng th\u1eddi d\u1ec5 d\u00e0ng \u0111\u1ec3 thi\u1ebft k\u1ebf \u0111\u1ea7u d\u00f2 taqman cho ph\u1ea3n \u1ee9ng Multiplex m\u00e0 kh\u00f4ng lo s\u1ef1 tr\u00f9ng l\u1eafp ph\u1ed5 hu\u1ef3nh quang gi\u1eefa ch\u1ea5t ph\u00e1t hu\u1ef3nh quang v\u00e0 ch\u1ea5t h\u1ea5p th\u1ee5.<\/p>\n\n\n\n

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V\u00ec sao l\u1ea1i s\u1eed d\u1ee5ng \u0111\u1ea7u d\u00f2 TaqMan trong b\u1ed9 kit ABT?<\/strong><\/h2>\n\n\n\n

Vi\u1ec7c ph\u00e1t hi\u1ec7n v\u00e0 \u0111\u1ecbnh l\u01b0\u1ee3ng m\u1ea7m b\u1ec7nh \u0111\u00f2i h\u1ecfi \u0111\u1ed9 nh\u1ea1y, \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u v\u00e0 \u0111\u1ed9 t\u00e1i l\u1eadp cao. Th\u00f4ng th\u01b0\u1eddng, c\u00e1c ch\u1ea5t hu\u1ef3nh quang g\u1eafn ch\u00e8n (SYBR Green) l\u00e0 nh\u1eefng ph\u00e2n t\u1eed nh\u1ecf n\u1eb1m xen k\u1ebd c\u00e1c s\u1ee3i DNA m\u1ea1ch \u0111\u00f4i m\u1edbi h\u00ecnh th\u00e0nh, d\u1eabn \u0111\u1ebfn vi\u1ec7c t\u0103ng c\u01b0\u1eddng t\u00edn hi\u1ec7u hu\u1ef3nh quang. \u01afu \u0111i\u1ec3m ch\u1ea5t hu\u1ef3nh quang g\u1eafn ch\u00e8n l\u00e0 chi ph\u00ed r\u1ebb, kh\u00f4ng c\u1ea7n thi\u1ebft k\u1ebf c\u00f3 th\u1ec3 theo d\u00f5i qu\u00e1 tr\u00ecnh khu\u1ebfch \u0111\u1ea1i c\u1ee7a s\u1ee3i DNA m\u1ea1ch \u0111\u00f4i m\u00e0 kh\u00f4ng c\u1ea7n \u0111\u1ea7u d\u00f2, \u0111i\u1ec1u n\u00e0y gi\u00fap gi\u1ea3m thi\u1ec3u chi ph\u00ed thi\u1ebft l\u1eadp v\u00e0 v\u1eadn h\u00e0nh th\u00ed nghi\u1ec7m. Tuy nhi\u00ean, b\u1ea5t k\u1ef3 s\u1ea3n ph\u1ea9m kh\u00f4ng \u0111\u1eb7c hi\u1ec7u n\u00e0o nh\u01b0 Primer-dimer t\u1ea1o ra trong ph\u1ea3n \u1ee9ng \u0111\u1ec1u c\u00f3 th\u1ec3 li\u00ean k\u1ebft v\u1edbi thu\u1ed1c nhu\u1ed9m d\u1eabn \u0111\u1ebfn k\u1ebft qu\u1ea3 d\u01b0\u01a1ng t\u00ednh gi\u1ea3 v\u00e0 \u0111\u1ecbnh l\u01b0\u1ee3ng kh\u00f4ng ch\u00ednh x\u00e1c. V\u00ec v\u1eady ch\u1ea5t hu\u1ef3nh quang g\u1eafn ch\u00e8n c\u00f3 nhi\u1ec1u h\u1ea1n ch\u1ebf trong qPCR \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh ch\u1ea5t l\u01b0\u1ee3ng bi\u1ec3u hi\u1ec7n gene v\u00e0 ch\u1ea9n \u0111o\u00e1n ph\u00e2n t\u1eed.<\/p>\n\n\n\n

H\u1ec7 th\u1ed1ng Real-time PCR \u0111\u01b0\u1ee3c c\u1ea3i thi\u1ec7n b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng \u0111\u1ea7u d\u00f2 \u0111\u00e1nh d\u1ea5u hu\u1ef3nh quang Taqman probe. \u0110\u1ea7u d\u00f2 c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c \u0111\u00e1nh d\u1ea5u b\u1eb1ng c\u00e1c ch\u1ea5t ph\u00e1t hu\u1ef3nh quang kh\u00e1c nhau cho ph\u00e9p khu\u1ebfch \u0111\u1ea1i \u0111\u1eb7c hi\u1ec7u c\u00e1c s\u1ea3n ph\u1ea9m khu\u1ebfch \u0111\u1ea1i trong ph\u1ea3n \u1ee9ng. \u0110i\u1ec1u n\u00e0y l\u00e0m t\u0103ng \u0111\u1ed9 nh\u1ea1y v\u00e0 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u gi\u1eefa \u0111\u1ea7u d\u00f2 v\u00e0 m\u1ee5c ti\u00eau \u0111\u1ec3 t\u1ea1o ra t\u00edn hi\u1ec7u hu\u1ef3nh quang. Do \u0111\u00f3, \u0111\u1ea7u d\u00f2 TaqMan \u0111\u00e3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 ph\u00e1t tri\u1ec3n ph\u01b0\u01a1ng ph\u00e1p Real-time PCR \u0111\u01a1n k\u00eanh v\u00e0 \u0111a k\u00eanh nh\u1eb1m ph\u00e1t hi\u1ec7n m\u1ed9t ho\u1eb7c nhi\u1ec1u t\u00e1c nh\u00e2n g\u00e2y b\u1ec7nh c\u00f9ng l\u00fac trong m\u1eabu. Ngo\u00e0i ra, \u0111\u1ea7u d\u00f2 Taqman \u1ee9ng d\u1ee5ng trong c\u00e1c th\u1eed nghi\u1ec7m li\u00ean quan \u0111\u1ebfn bi\u1ec3u hi\u1ec7n gene, \u0111\u1ecbnh l\u01b0\u1ee3ng DNA, Chip, X\u00e1c \u0111\u1ecbnh ki\u1ec3u gene SNP, Ph\u00e2n t\u00edch con \u0111\u01b0\u1eddng microRNA v\u00e0 RNA nh\u1ecf, ph\u00e1t hi\u1ec7n \u0111\u1ed9t bi\u1ebfn v\u00e0 ph\u00e2n t\u00edch protein.<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

ABT s\u1eed d\u1ee5ng \u0111\u1ea7u d\u00f2 Taqman trong ph\u00e1t tri\u1ec3n c\u00e1c b\u1ed9 kit Real-time PCR nh\u1eb1m ch\u1ea9n \u0111o\u00e1n b\u1ec7nh \u1edf ng\u01b0\u1eddi, th\u00fa y, th\u1ee7y s\u1ea3n, vi sinh th\u1ef1c ph\u1ea9m v\u00e0 GMO. C\u00e1c ch\u1ea5t ph\u00e1t quang th\u01b0\u1eddng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng bao g\u1ed3m k\u00eanh FAM, HEX, Texas Red, v\u00e0 Cy5. \u0110\u00e2y l\u00e0 c\u00e1c ch\u1ea5t  c\u00f3 b\u01b0\u1edbc s\u00f3ng hu\u1ef3nh quang t\u1ed1i \u0111a c\u00e1ch xa nhau nh\u1ea5t v\u00e0 ph\u00f9 h\u1ee3p v\u1edbi ngu\u1ed3n s\u00e1ng k\u00edch th\u00edch v\u00e0 hu\u1ef3nh quang ph\u00e1t ra t\u1eeb m\u00e1y Real-time PCR. T\u1ed5ng th\u1eddi gian th\u1ef1c hi\u1ec7n trung b\u00ecnh l\u00e0 1 gi\u1edd 30 ph\u00fat. C\u00e1c b\u1ed9 kit TaqMan qPCR \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf \u0111\u1eb7c hi\u1ec7u v\u1edbi tr\u00ecnh t\u1ef1 m\u1ee5c ti\u00eau, s\u1eed d\u1ee5ng ho\u1ea1t t\u00ednh nuclease 5′ c\u1ee7a Taq Polymerase v\u00e0 \u0111\u1ea7u d\u00f2 Taqman \u0111\u1ec3 t\u0103ng \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u cho vi\u1ec7c ph\u00e1t hi\u1ec7n DNA m\u1ee5c ti\u00eau. \u0110i\u1ec1u n\u00e0y mang l\u1ea1i k\u1ebft qu\u1ea3 ch\u00ednh x\u00e1c, \u0111\u1ed9 nh\u1ea1y cao, d\u1ec5 s\u1eed d\u1ee5ng v\u00e0 h\u1ea1n ch\u1ebf nguy c\u01a1 ngo\u1ea1i nhi\u1ec5m.<\/p>\n\n\n\n

<\/div>\n\n\n\n

T\u00e0i li\u1ec7u tham kh\u1ea3o<\/strong><\/h2>\n\n\n\n

[1] Artika, I. M., Dewi, Y. P., Nainggolan, I. M., Siregar, J. E., & Antonjaya, U. (2022). Real-Time Polymerase Chain Reaction: Current Techniques, Applications, and Role in COVID-19 Diagnosis. Genes, 13(12), 2387. https:\/\/doi.org\/10.3390\/genes13122387<\/a><\/p>\n\n\n\n

[2] Zhang, M., Liu, K., Hu, Y. et al. A novel quantitative PCR mediated by high-fidelity DNA polymerase. Sci Rep 7, 10365 (2017). https:\/\/doi.org\/10.1038\/s41598-017-10782-4<\/a><\/p>\n\n\n\n

[3] ThermoFisher Sciencetific. TaqMan vs. SYBR Chemistry for Real-Time PCR. ThermoFisher Sciencetific. https:\/\/www.thermofisher.com\/vn\/en\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-learning-center\/real-time-pcr-basics\/taqman-vs-sybr-chemistry-real-time -pcr.<\/a><\/p>\n\n\n\n

[4] GeneLink. Fluorescent Dyes Applications. https:\/\/www.genelink.com\/oligo_modifications_reference\/OMR_mod_category_applications.asp?mod_sp_cat_id=18<\/a><\/p>\n\n\n\n

[5] C\u00d4NG TY TNHH GI\u1ea2I PH\u00c1P Y SINH ABT. (2023). T\u1ed5ng H\u1ee3p Ki\u1ebfn Th\u1ee9c Chi Ti\u1ebft V\u1ec1 K\u1ef9 Thu\u1eadt Real-Time PCR. https:\/\/abtvn.com\/real-time-pcr\/<\/a><\/p>","protected":false},"excerpt":{"rendered":"

Real-time PCR l\u00e0 g\u00ec? Real-time PCR, hay c\u00f2n g\u1ecdi l\u00e0 PCR \u0111\u1ecbnh l\u01b0\u1ee3ng theo th\u1eddi gian th\u1ef1c (qPCR), \u0111\u01b0\u1ee3c ph\u00e1t tri\u1ec3n v\u00e0o n\u0103m 1993 \u0111\u1ec3 ph\u00e1t hi\u1ec7n v\u00e0 \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic. C\u00f4ng ngh\u1ec7 n\u00e0y \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng r\u1ed9ng r\u00e3i trong h\u1ea7u h\u1ebft c\u00e1c l\u0129nh v\u1ef1c nghi\u00ean c\u1ee9u y sinh, n\u00f4ng nghi\u1ec7p, th\u1ef1c ph\u1ea9m v\u00e0 […]<\/p>","protected":false},"author":1,"featured_media":11381,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[228,270,266,267],"tags":[498],"class_list":["post-11346","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-bai-viet","category-kien-thuc","category-sinh-hoc-phan-tu","category-sinh-hoc-phan-tu-bai-viet","tag-taqman-probe"],"uagb_featured_image_src":{"full":["https:\/\/abtvn.com\/wp-content\/uploads\/2024\/06\/Banner-blog-taqman-probe.png",3840,2160,false],"thumbnail":["https:\/\/abtvn.com\/wp-content\/uploads\/2024\/06\/Banner-blog-taqman-probe-150x150.png",150,150,true],"medium":["https:\/\/abtvn.com\/wp-content\/uploads\/2024\/06\/Banner-blog-taqman-probe-300x169.png",300,169,true],"medium_large":["https:\/\/abtvn.com\/wp-content\/uploads\/2024\/06\/Banner-blog-taqman-probe-768x432.png",768,432,true],"large":["https:\/\/abtvn.com\/wp-content\/uploads\/2024\/06\/Banner-blog-taqman-probe-1024x576.png",1024,576,true],"1536x1536":["https:\/\/abtvn.com\/wp-content\/uploads\/2024\/06\/Banner-blog-taqman-probe-1536x864.png",1536,864,true],"2048x2048":["https:\/\/abtvn.com\/wp-content\/uploads\/2024\/06\/Banner-blog-taqman-probe-2048x1152.png",2048,1152,true],"trp-custom-language-flag":["https:\/\/abtvn.com\/wp-content\/uploads\/2024\/06\/Banner-blog-taqman-probe-18x10.png",18,10,true],"woocommerce_thumbnail":["https:\/\/abtvn.com\/wp-content\/uploads\/2024\/06\/Banner-blog-taqman-probe-1707x960.png",1707,960,true],"woocommerce_single":["https:\/\/abtvn.com\/wp-content\/uploads\/2024\/06\/Banner-blog-taqman-probe-2560x1440.png",2560,1440,true],"woocommerce_gallery_thumbnail":["https:\/\/abtvn.com\/wp-content\/uploads\/2024\/06\/Banner-blog-taqman-probe-100x100.png",100,100,true]},"uagb_author_info":{"display_name":"ABT Team","author_link":"https:\/\/abtvn.com\/en\/author\/admin\/"},"uagb_comment_info":0,"uagb_excerpt":"Real-time PCR l\u00e0 g\u00ec? Real-time PCR, hay c\u00f2n g\u1ecdi l\u00e0 PCR \u0111\u1ecbnh l\u01b0\u1ee3ng theo th\u1eddi gian th\u1ef1c (qPCR), \u0111\u01b0\u1ee3c ph\u00e1t tri\u1ec3n v\u00e0o n\u0103m 1993 \u0111\u1ec3 ph\u00e1t hi\u1ec7n v\u00e0 \u0111\u1ecbnh l\u01b0\u1ee3ng axit nucleic. C\u00f4ng ngh\u1ec7 n\u00e0y \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng r\u1ed9ng r\u00e3i trong h\u1ea7u h\u1ebft c\u00e1c l\u0129nh v\u1ef1c nghi\u00ean c\u1ee9u y sinh, n\u00f4ng nghi\u1ec7p, th\u1ef1c ph\u1ea9m v\u00e0…","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/11346","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=11346"}],"version-history":[{"count":6,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/11346\/revisions"}],"predecessor-version":[{"id":13080,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/11346\/revisions\/13080"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/11381"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=11346"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/categories?post=11346"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/tags?post=11346"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}