{"id":10601,"date":"2024-04-27T10:46:22","date_gmt":"2024-04-27T03:16:22","guid":{"rendered":"https:\/\/abtvn.com\/?p=10601"},"modified":"2025-08-06T14:18:13","modified_gmt":"2025-08-06T06:48:13","slug":"tach-chiet-bang-hat-tu-tinh-tu-thu-cong-den-tu-dong-hoa","status":"publish","type":"post","link":"https:\/\/abtvn.com\/en\/tach-chiet-bang-hat-tu-tinh-tu-thu-cong-den-tu-dong-hoa\/","title":{"rendered":"Ph\u01b0\u01a1ng ph\u00e1p t\u00e1ch chi\u1ebft nucleic acid b\u1eb1ng h\u1ea1t t\u1eeb t\u00ednh t\u1eeb th\u1ee7 c\u00f4ng \u0111\u1ebfn t\u1ef1 \u0111\u1ed9ng h\u00f3a<\/strong>"},"content":{"rendered":"

M\u1ed9t quy tr\u00ecnh ch\u1ea9n \u0111o\u00e1n ph\u00e2n t\u1eed ho\u00e0n ch\u1ec9nh s\u1ebd bao g\u1ed3m thu m\u1eabu, x\u1eed l\u00fd m\u1eabu, t\u00e1ch chi\u1ebft nucleic acid, ph\u00e2n t\u00edch ph\u00e2n t\u1eed v\u00e0 \u0111\u00e1nh gi\u00e1, di\u1ec5n gi\u1ea3i k\u1ebft qu\u1ea3. Trong \u0111\u00f3, b\u01b0\u1edbc t\u00e1ch chi\u1ebft s\u1ebd ph\u00e2n l\u1eadp v\u00e0 l\u00e0m s\u1ea1ch ph\u00e2n t\u1eed nucleic acid m\u1ee5c ti\u00eau. \u0110\u00e2y \u0111\u01b0\u1ee3c coi l\u00e0 b\u01b0\u1edbc kh\u00f4ng th\u1ec3 thi\u1ebfu trong x\u00e9t nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed.<\/p>\n\n\n\n

Nucleic acid sau khi \u0111\u01b0\u1ee3c ly tr\u00edch s\u1ebd tr\u1edf th\u00e0nh ngu\u1ed3n nguy\u00ean li\u1ec7u \u0111\u1ea7u v\u00e0o v\u00e0 quy\u1ebft \u0111\u1ecbnh ch\u1ea5t l\u01b0\u1ee3ng k\u1ebft qu\u1ea3 cho c\u00e1c th\u00ed nghi\u1ec7m ti\u1ebfp theo sau \u0111\u00f3 nh\u01b0 PCR, gi\u1ea3i tr\u00ecnh t\u1ef1, Western blotting hay microarray. T\u00f9y theo lo\u1ea1i m\u1eabu, m\u1ee5c \u0111\u00edch v\u00e0 y\u00eau c\u1ea7u \u0111\u1ed1i v\u1edbi nucleic acid sau ly tr\u00edch, c\u00f3 th\u1ec3 c\u00f3 nhi\u1ec1u ph\u01b0\u01a1ng ph\u00e1p ly tr\u00edch kh\u00e1c nhau. Hi\u1ec7n nay, c\u00f3 nhi\u1ec1u ph\u01b0\u01a1ng ph\u00e1p t\u00e1ch chi\u1ebft nh\u01b0 d\u00f9ng phenol-chloroform, c\u1ed9t silica, h\u1ea1t t\u1eeb t\u00ednh, \u2026<\/p>\n\n\n\n

Trong \u0111\u00f3, h\u1ea1t t\u1eeb \u0111\u01b0\u1ee3c coi l\u00e0 nguy\u00ean li\u1ec7u hi\u1ec7u qu\u1ea3 trong ph\u00e1t tri\u1ec3n c\u00e1c ph\u01b0\u01a1ng ph\u00e1p t\u00e1ch chi\u1ebft t\u1eeb<\/a><\/strong> th\u1ee7 c\u00f4ng \u0111\u1ebfn t\u1ef1 \u0111\u1ed9ng. V\u1edbi m\u1ed7i ph\u01b0\u01a1ng ph\u00e1p t\u00e1ch chi\u1ebft d\u00f9 l\u00e0 th\u1ee7 c\u00f4ng hay t\u1ef1 \u0111\u1ed9ng \u0111\u1ec1u s\u1ebd c\u00f3 \u01b0u nh\u01b0\u1ee3c \u0111i\u1ec3m ri\u00eang. Do v\u1eady, vi\u1ec7c l\u1ef1a ch\u1ecdn ph\u01b0\u01a1ng ph\u00e1p t\u00e1ch h\u1ea1t t\u1eeb ph\u1ee5 thu\u1ed9c v\u00e0o y\u00eau c\u1ea7u c\u1ee5 th\u1ec3 c\u1ee7a \u1ee9ng d\u1ee5ng, \u0111i\u1ec1u ki\u1ec7n th\u00ed nghi\u1ec7m, kh\u1ea3 n\u0103ng c\u1ee7a k\u1ef9 thu\u1eadt vi\u00ean v\u00e0 m\u1ee9c \u0111\u1ed9 t\u1ef1 \u0111\u1ed9ng h\u00f3a mong mu\u1ed1n. Tuy nhi\u00ean, c\u1ea3 hai ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ec1u \u0111\u00f3ng vai tr\u00f2 quan tr\u1ecdng trong vi\u1ec7c c\u1ea3i thi\u1ec7n hi\u1ec7u su\u1ea5t v\u00e0 hi\u1ec7u qu\u1ea3 c\u1ee7a c\u00e1c x\u00e9t nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed.<\/p>\n\n\n\n

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1. T\u00ednh n\u0103ng c\u1ee7a h\u1ea1t t\u1eeb t\u00ednh<\/strong><\/h2>\n\n\n\n

Hi\u1ec7n nay, c\u00f4ng ngh\u1ec7 nano \u0111ang ng\u00e0y c\u00e0ng ph\u00e1t tri\u1ec3n v\u00e0 \u0111\u1ea1t \u0111\u01b0\u1ee3c nhi\u1ec1u th\u00e0nh t\u1ef1u to l\u1edbn kh\u00f4ng ch\u1ec9 trong l\u0129nh v\u1ef1c nghi\u00ean c\u1ee9u m\u00e0 c\u00f2n m\u1edf r\u1ed9ng ph\u1ea1m vi \u1ee9ng d\u1ee5ng trong nhi\u1ec1u l\u0129nh v\u1ef1c kh\u00e1c nhau.<\/p>\n\n\n\n

Trong \u0111\u00f3, h\u1ea1t nano oxit s\u1eaft t\u1eeb \u0111\u01b0\u1ee3c \u1ee9ng d\u1ee5ng r\u1ed9ng r\u00e3i trong c\u00e1c l\u0129nh v\u1ef1c khoa h\u1ecdc k\u1ef9 thu\u1eadt, x\u1eed l\u00fd m\u00f4i tr\u01b0\u1eddng v\u00e0 \u0111\u1eb7c bi\u1ec7t l\u00e0 l\u0129nh v\u1ef1c y sinh. Hai lo\u1ea1i h\u1ea1t oxit s\u1eaft ph\u1ed5 bi\u1ebfn \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng l\u00e0 maghemite (\u03b3 -Fe2<\/sub>O3<\/sub>) v\u00e0 magnetite (Fe3<\/sub>O4<\/sub>), v\u1edbi k\u00edch th\u01b0\u1edbc ch\u1ec9 kho\u1ea3ng 20 \u0111\u1ebfn 30 nm. Ch\u00fang l\u00e0 c\u00e1c v\u1eadt li\u1ec7u nano n\u1ed5i b\u1eadt v\u1edbi c\u00e1c t\u00ednh ch\u1ea5t nh\u01b0 t\u00ednh si\u00eau thu\u1eadn t\u1eeb, t\u00ednh t\u01b0\u01a1ng th\u00edch sinh h\u1ecdc, t\u00ednh \u1ed5n \u0111\u1ecbnh, thu\u1ed9c t\u00ednh h\u00f3a l\u00fd v\u00e0 c\u00e1c thu\u1ed9c t\u00ednh c\u00f3 li\u00ean quan kh\u00e1c. C\u00e1c h\u1ea1t n\u00e0y \u0111\u01b0\u1ee3c b\u1ecdc m\u1ed9t l\u1edbp ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t, cho ph\u00e9p ch\u00fang ph\u00e2n t\u00e1n trong m\u1ed9t dung m\u00f4i \u0111\u1ed3ng nh\u1ea5t \u0111\u01b0\u1ee3c g\u1ecdi l\u00e0 ch\u1ea5t l\u1ecfng t\u1eeb. C\u00e1c hi\u1ec7u \u1ee9ng l\u01b0\u1ee3ng t\u1eed t\u1eeb vi\u1ec7c gi\u1ea3m k\u00edch th\u01b0\u1edbc v\u00e0 di\u1ec7n t\u00edch b\u1ec1 m\u1eb7t l\u1edbn c\u1ee7a c\u00e1c h\u1ea1t nano \u0111\u00e3 thay \u0111\u1ed5i m\u1ed9t s\u1ed1 \u0111\u1eb7c t\u00ednh t\u1eeb \u0111\u1eb7c tr\u01b0ng c\u1ee7a v\u1eadt li\u1ec7u, l\u00e0m n\u1ed5i b\u1eadt hi\u1ec7n t\u01b0\u1ee3ng si\u00eau thu\u1eadn t\u1eeb. <\/p>\n\n\n\n

\u0110\u1eb7c t\u00ednh n\u00e0y gi\u00fap c\u00e1c h\u1ea1t si\u00eau thu\u1eadn t\u1eeb ch\u1ec9 th\u1ec3 hi\u1ec7n t\u00ednh t\u1eeb khi c\u00f3 t\u1eeb tr\u01b0\u1eddng b\u00ean ngo\u00e0i v\u00e0 l\u00e0m n\u1ed5i b\u1eadt s\u1ef1 kh\u00e1c bi\u1ec7t so v\u1edbi c\u00e1c lo\u1ea1i s\u1eaft t\u1eeb ph\u1ed5 bi\u1ebfn kh\u00e1c. \u0110i\u1ec1u n\u00e0y gi\u00fap h\u1ea1n ch\u1ebf t\u1ed1i \u0111a s\u1ef1 k\u1ebft t\u1ee5 kh\u00f4ng mong mu\u1ed1n khi \u1edf ngo\u00e0i l\u1ef1c t\u1eeb tr\u01b0\u1eddng. Ngo\u00e0i ra, k\u00edch th\u01b0\u1edbc nh\u1ecf cho ph\u00e9p c\u00e1c h\u1ea1t n\u00e0y tr\u1edf n\u00ean t\u00e1ch bi\u1ec7t khi \u1edf d\u1ea1ng huy\u1ec1n ph\u00f9 trong dung d\u1ecbch.<\/p>\n\n\n\n

V\u1ec1 c\u1ea5u tr\u00fac, ph\u1ea7n l\u00f5i c\u1ee7a c\u00e1c h\u1ea1t t\u1eeb l\u00e0 oxit s\u1eaft v\u00e0 \u0111\u01b0\u1ee3c bao ph\u1ee7 b\u1edfi c\u00e1c l\u1edbp v\u1ecf ch\u1ee9c n\u0103ng kh\u00e1c nhau, t\u1ea1o ra c\u00e1c \u0111\u1eb7c t\u00ednh li\u00ean k\u1ebft \u0111\u1eb7c bi\u1ec7t cho h\u1ea1t t\u1eeb. B\u1ec1 m\u1eb7t c\u1ee7a c\u00e1c h\u1ea1t n\u00e0y \u0111\u01b0\u1ee3c \u0111i\u1ec1u ch\u1ec9nh th\u00f4ng qua vi\u1ec7c b\u1ecdc m\u1ed9t v\u00e0i l\u1edbp nguy\u00ean t\u1eed c\u1ee7a c\u00e1c polime h\u1eefu c\u01a1, kim lo\u1ea1i (Au), c\u00e1c oxit v\u00f4 c\u01a1 (nh\u01b0 SiO2<\/sub>, Al2<\/sub>O3<\/sub>) v\u00e0 c\u0169ng c\u00f3 th\u1ec3 ch\u1ee9c h\u00f3a b\u1eb1ng c\u00e1ch g\u1eafn c\u00e1c ph\u00e2n t\u1eed c\u00f3 ho\u1ea1t t\u00ednh sinh h\u1ecdc kh\u00e1c nhau. B\u00ean c\u1ea1nh \u0111\u00f3, l\u1edbp v\u1ecf gi\u00fap tr\u00e1nh s\u1ef1 k\u1ebft t\u1ee5, gi\u1ea3m s\u1ef1 l\u1eafng \u0111\u1ecdng, h\u1ea1n ch\u1ebf s\u1ef1 oxy h\u00f3a c\u1ee7a h\u1ea1t nano c\u0169ng nh\u01b0 t\u1ea1o cho b\u1ec1 m\u1eb7t c\u1ee7a ch\u00fang c\u00f3 nh\u1eefng \u0111\u1eb7c th\u00f9 kh\u00e1c bi\u1ec7t \u0111\u1ec3 ph\u00f9 h\u1ee3p v\u1edbi t\u1eebng \u1ee9ng d\u1ee5ng kh\u00e1c nhau.<\/p>\n\n\n\n

D\u1ef1a tr\u00ean c\u00e1c \u0111\u1eb7c t\u00ednh \u0111\u00f3 c\u00e1c h\u1ea1t nano si\u00eau thu\u1eadn t\u1eeb m\u1edf ra ti\u1ec1m n\u0103ng l\u1edbn cho nh\u1eefng \u1ee9ng d\u1ee5ng trong l\u0129nh v\u1ef1c y sinh, bao g\u1ed3m tinh s\u1ea1ch c\u00e1c ph\u00e2n t\u1eed sinh h\u1ecdc, t\u0103ng \u0111\u1ed9 t\u01b0\u01a1ng ph\u1ea3n trong m\u00e1y c\u1ed9ng h\u01b0\u1edfng t\u1eeb h\u1ea1t nh\u00e2n, ph\u00e2n t\u00e1ch v\u00e0 ch\u1ecdn l\u1ecdc t\u1ebf b\u00e0o, hi\u1ec7u \u1ee9ng nhi\u1ec7t v\u00e0 ph\u00e2n ph\u00e1t thu\u1ed1c. Trong \u0111\u00f3, h\u1ea1t t\u1eeb t\u00ednh ph\u1ee7 silica \u0111\u00e3 \u0111\u01b0\u1ee3c \u1ee9ng d\u1ee5ng m\u1ed9t c\u00e1ch hi\u1ec7u qu\u1ea3 trong vi\u1ec7c ly tr\u00edch v\u00e0 tinh s\u1ea1ch nucleic acid t\u1eeb c\u00e1c m\u1eabu th\u00f4 v\u00e0 t\u1eeb nhi\u1ec1u lo\u1ea1i m\u1eabu kh\u00e1c nhau.<\/p>\n\n\n\n

\"H\u00ecnh
Figure 1. <\/strong>H\u1ea1t t\u1eeb t\u00ednh ph\u1ee7 silica<\/em><\/figcaption><\/figure>\n\n\n\n

2. Nguy\u00ean t\u1eafc ph\u01b0\u01a1ng ph\u00e1p t\u00e1ch chi\u1ebft nucleic acid b\u1eb1ng h\u1ea1t t\u1eeb t\u00ednh<\/strong><\/h2>\n\n\n\n

Trong t\u00e1ch chi\u1ebft nucleic acid, h\u1ea1t t\u1eeb t\u00ednh v\u1edbi l\u00f5i oxit s\u1eaft \u0111\u01b0\u1ee3c bao ph\u1ee7 b\u1ec1 m\u1eb7t v\u1edbi silica l\u00e0 v\u1eadt li\u1ec7u ph\u00f9 h\u1ee3p \u0111\u1ec3 g\u1eafn DNA\/RNA. Silica v\u1edbi \u0111\u1eb7c t\u00ednh kh\u00f4ng \u0111\u1ed9c h\u1ea1i c\u00f3 kh\u1ea3 n\u0103ng t\u1ea1o polymer li\u00ean k\u1ebft ngang r\u1ed9ng r\u00e3i, h\u00ecnh th\u00e0nh m\u1ed9t l\u1edbp v\u1ecf b\u1ea3o v\u1ec7 tr\u01a1 b\u00ean ngo\u00e0i c\u00e1c h\u1ea1t nano v\u00e0 ch\u00fang r\u1ea5t t\u01b0\u01a1ng th\u00edch sinh h\u1ecdc b\u1edfi silica th\u01b0\u1eddng \u0111\u01b0\u1ee3c k\u1ebft th\u00fac b\u1eb1ng m\u1ed9t nh\u00f3m silanol c\u00f3 th\u1ec3 ph\u1ea3n \u1ee9ng v\u1edbi c\u00e1c t\u00e1c nh\u00e2n kh\u00e1c nhau \u0111\u1ec3 g\u1eafn c\u1ed9ng h\u00f3a tr\u1ecb c\u00e1c ph\u1ed1i t\u1eed c\u1ee5 th\u1ec3 v\u00e0o b\u1ec1 m\u1eb7t c\u1ee7a c\u00e1c h\u1ea1t nano t\u1eeb t\u00ednh n\u00e0y.<\/p>\n\n\n\n

H\u1ea1t t\u1eeb ph\u1ee7 silica c\u00f3 \u01b0u \u0111i\u1ec3m l\u00e0 x\u1ed1p, di\u1ec7n t\u00edch b\u1ec1 m\u1eb7t ri\u00eang l\u1edbn, kh\u00f4ng \u0111\u1ed9c v\u00e0 kh\u00e1 tr\u01a1 v\u1ec1 m\u1eb7t h\u00f3a h\u1ecdc, v\u00ec v\u1eady silica c\u00f3 kh\u1ea3 n\u0103ng h\u1ea5p ph\u1ee5 v\u00e0 kh\u1ea3 n\u0103ng \u1ee9ng d\u1ee5ng cao. So v\u1edbi c\u00e1c h\u1ea1t nano kh\u00e1c, h\u1ea1t t\u1eeb ph\u1ee7 silica c\u00f3 \u01b0u \u0111i\u1ec3m l\u00e0 d\u1ec5 ph\u00e2n t\u00e1n h\u01a1n trong c\u00e1c polymer. V\u1edbi nh\u1eefng t\u00ednh ch\u1ea5t \u0111\u1eb7c tr\u01b0ng \u0111\u00f3 c\u1ee7a h\u1ea1t t\u1eeb ph\u1ee7 silica \u0111\u00e3 \u0111\u01b0\u1ee3c \u1ee9ng d\u1ee5ng hi\u1ec7u qu\u1ea3 trong t\u00e1ch chi\u1ebft nucleic acid. <\/p>\n\n\n\n

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Quy tr\u00ecnh t\u00e1ch chi\u1ebft nucleic acid b\u1eb1ng h\u1ea1t t\u1eeb t\u00ednh bao g\u1ed3m 4 b\u01b0\u1edbc ch\u00ednh: (1) ly gi\u1ea3i t\u1ebf b\u00e0o, (2) c\u1ed1 \u0111\u1ecbnh nucleic acid l\u00ean b\u1ec1 m\u1eb7t h\u1ea1t t\u1eeb, (3) lo\u1ea1i b\u1ecf t\u1ea1p ch\u1ea5t, (4) thu nh\u1eadn nucleic acid <\/em><\/strong><\/p>\n\n\n\n

(1) Ly gi\u1ea3i t\u1ebf b\u00e0o:<\/strong> M\u1eabu \u0111\u01b0\u1ee3c ly gi\u1ea3i \u0111\u1ec3 ph\u00e1 v\u1ee1 m\u00e0ng t\u1ebf b\u00e0o v\u00e0 gi\u1ea3i ph\u00f3ng nucleic acid b\u1eb1ng c\u00e1c ch\u1ea5t ph\u00e1 m\u00e0ng nh\u01b0 Triton X-100, SDS, Tween 20, proteinase K, \u2026<\/p>\n\n\n\n

(2) C\u1ed1 \u0111\u1ecbnh nucleic acid l\u00ean b\u1ec1 m\u1eb7t h\u1ea1t t\u1eeb:<\/strong> Mu\u1ed1i trao \u0111\u1ed5i ion \u1edf n\u1ed3ng \u0111\u1ed9 cao hay c\u00f2n g\u1ecdi l\u00e0 mu\u1ed1i chaotropic (guanidine hydrochloride, guanidine thiocyanate, urea,\u2026) v\u00e0 ethanol \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 l\u00e0m t\u0103ng t\u00ednh k\u1ef5 n\u01b0\u1edbc c\u1ee7a nucleic acid. L\u00fac n\u00e0y trong dung d\u1ecbch h\u00ecnh th\u00e0nh c\u00e1c c\u1ea7u n\u1ed1i cation gi\u1eefa DNA\/RNA v\u00e0 b\u1ec1 m\u1eb7t silica c\u1ee7a h\u1ea1t t\u1eeb (H\u00ecnh 2)<\/em><\/strong>. Th\u1eddi gian h\u00fat\/nh\u1ea3 h\u1ea1t t\u1eeb c\u0169ng \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn hi\u1ec7u qu\u1ea3 t\u00e1ch chi\u1ebft<\/p>\n\n\n\n

(3) Lo\u1ea1i b\u1ecf t\u1ea1p ch\u1ea5t:<\/strong> C\u00e1c th\u00e0nh ph\u1ea7n nh\u01b0 protein, lipid v\u00e0 c\u00e1c t\u1ea1p ch\u1ea5t kh\u00e1c s\u1ebd \u0111\u01b0\u1ee3c lo\u1ea1i b\u1ecf b\u1eb1ng c\u00e1c dung d\u1ecbch r\u1eeda. Trong qu\u00e1 tr\u00ecnh r\u1eeda v\u1eabn ph\u1ea3i \u0111\u1ea3m b\u1ea3o s\u1ef1 g\u1eafn ch\u1eb7t c\u1ee7a nucleic acid v\u00e0o m\u00e0ng silica n\u00ean trong c\u00e1c dung d\u1ecbch r\u1eeda th\u01b0\u1eddng b\u1ed5 sung ethanol.<\/p>\n\n\n\n

(4) Thu nh\u1eadn nucleic acid:<\/strong> B\u01b0\u1edbc n\u00e0y nucleic acid \u0111\u01b0\u1ee3c v\u00e0o dung d\u1ecbch \u0111\u1ec7m \u0111\u1ec3 ph\u00e1 v\u1ee1 li\u00ean k\u1ebft v\u1edbi m\u00e0ng silica. Theo \u0111\u00f3, n\u01b0\u1edbc x\u1eed l\u00fd DEPC cho RNA ho\u1eb7c dung d\u1ecbch TE c\u00f3 pH 8-9 \u0111\u1ec3 thu nh\u1eadn v\u00e0 b\u1ea3o qu\u1ea3n DNA.<\/p>\n\n\n\n

Nh\u01b0 v\u1eady, c\u01a1 ch\u1ebf c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p t\u00e1ch chi\u1ebft nucleic acid b\u1eb1ng h\u1ea1t t\u1eeb ph\u1ee7 silica d\u1ef1a tr\u00ean s\u1ef1 t\u01b0\u01a1ng t\u00e1c gi\u1eefa nucleic acid v\u00e0 silica \u1edf \u0111i\u1ec1u ki\u1ec7n pH v\u00e0 n\u1ed3ng \u0111\u1ed9 mu\u1ed1i cao. H\u1ea1t t\u1eeb ph\u1ee7 silica c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c \u0111i\u1ec1u ch\u1ec9nh k\u00edch th\u01b0\u1edbc v\u00e0 h\u00f3a t\u00ednh b\u1ec1 m\u1eb7t, t\u1ea1o \u0111i\u1ec1u ki\u1ec7n thu\u1eadn l\u1ee3i cho t\u00e1ch chi\u1ebft nucleic acid tr\u00ean nhi\u1ec1u lo\u1ea1i n\u1ec1n m\u1eabu kh\u00e1c nhau. Tr\u01b0\u1edbc \u0111\u00e2y, ph\u01b0\u01a1ng ph\u00e1p n\u00e0y c\u0169ng \u0111\u01b0\u1ee3c ti\u1ebfn h\u00e0nh th\u1ee7 c\u00f4ng ho\u00e0n to\u00e0n nh\u01b0 c\u00e1c ph\u01b0\u01a1ng ph\u00e1p truy\u1ec1n th\u1ed1ng kh\u00e1c, \u01b0u \u0111i\u1ec3m l\u1edbn c\u1ee7a t\u00e1ch chi\u1ebft h\u1ea1t t\u1eeb th\u1ee7 c\u00f4ng l\u00e0 d\u1ec5 d\u00e0ng th\u1ef1c hi\u1ec7n, d\u1ee5ng c\u1ee5 th\u00ed nghi\u1ec7m \u0111\u01a1n gi\u1ea3n, \u00edt \u0111\u1ed9c h\u1ea1i, kh\u00f4ng y\u00eau c\u1ea7u m\u00e1y ly t\u00e2m tr\u1ee3 l\u1ef1c v\u00e0 c\u00f3 th\u1ec3 th\u1ef1c hi\u1ec7n ngo\u00e0i th\u1ef1c \u0111\u1ecba.<\/p>\n\n\n\n

Sau n\u00e0y v\u1edbi s\u1ef1 ph\u00e1t tri\u1ec3n c\u1ee7a c\u00f4ng ngh\u1ec7 t\u1ef1 \u0111\u1ed9ng h\u00f3a, h\u1ea1t t\u1eeb ph\u1ee7 silica \u0111\u01b0\u1ee3c \u1ee9ng d\u1ee5ng v\u00e0o c\u00e1c thi\u1ebft b\u1ecb trong qu\u00e1 tr\u00ecnh t\u00e1ch chi\u1ebft nucleic acid gi\u00fap gia t\u0103ng hi\u1ec7u su\u1ea5t v\u00e0 \u0111\u1ed9 ch\u00ednh x\u00e1c cao h\u01a1n, \u0111\u1ed3ng th\u1eddi gi\u1ea3m thi\u1ec3u s\u1ef1 can thi\u1ec7p c\u1ee7a con ng\u01b0\u1eddi. D\u00f9 l\u00e0 h\u00ecnh th\u1ee9c th\u1ef1c hi\u1ec7n th\u1ee7 c\u00f4ng hay t\u1ef1 \u0111\u1ed9ng, ph\u01b0\u01a1ng ph\u00e1p t\u00e1ch chi\u1ebft nucleic acid b\u1eb1ng h\u1ea1t t\u1eeb \u0111ang \u0111\u01b0\u1ee3c \u1ee9ng d\u1ee5ng r\u1ed9ng r\u00e3i trong th\u00ed nghi\u1ec7m v\u1ec1 sinh h\u1ecdc ph\u00e2n t\u1eed.<\/p>\n\n\n\n

\"H\u00ecnh
Figure 2. <\/strong>H\u1ea1t t\u1eeb t\u00ednh ph\u1ee7 silica h\u00ecnh th\u00e0nh li\u00ean k\u1ebft v\u1edbi nucleic acid<\/em><\/figcaption><\/figure>\n\n\n\n

3. Ph\u01b0\u01a1ng ph\u00e1p t\u00e1ch chi\u1ebft h\u1ea1t t\u1eeb th\u1ee7 c\u00f4ng<\/strong><\/h2>\n\n\n\n

Qu\u00e1 tr\u00ecnh th\u1ef1c hi\u1ec7n t\u00e1ch chi\u1ebft nucleic acid b\u1eb1ng h\u1ea1t t\u1eeb theo d\u1ea1ng th\u1ee7 c\u00f4ng \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n ho\u00e0n to\u00e0n b\u1eb1ng tay, t\u1eeb chu\u1ea9n b\u1ecb m\u1eabu \u0111\u1ebfn thu nh\u1eadn nucleic acid. H\u00ecnh 3 m\u00f4 t\u1ea3 qu\u00e1 tr\u00ecnh th\u1ef1c hi\u1ec7n t\u00e1ch chi\u1ebft nucleic acid th\u1ee7 c\u00f4ng b\u1eb1ng h\u1ea1t t\u1eeb t\u00ednh. \u0110\u1ea7u ti\u00ean, m\u1eabu \u0111\u01b0\u1ee3c h\u00f2a tr\u1ed9n v\u1edbi dung d\u1ecbch ly gi\u1ea3i \u0111\u1ec3 gi\u1ea3i ph\u00f3ng nucleic acid ra kh\u1ecfi t\u1ebf b\u00e0o. Ti\u1ebfp theo, h\u1ea1t t\u1eeb s\u1ebd \u0111\u01b0\u1ee3c cho v\u00e0o tube m\u1eabu \u0111\u00e3 ly gi\u1ea3i, DNA\/RNA l\u00fac n\u00e0y s\u1ebd g\u1eafn k\u1ebft l\u00ean b\u1ec1 m\u1eb7t h\u1ea1t t\u1eeb v\u1edbi s\u1ef1 c\u00f3 m\u1eb7t c\u1ee7a mu\u1ed1i v\u00e0 ethanol. Sau \u0111\u00f3, c\u00e1c tube dung d\u1ecbch s\u1ebd \u0111\u01b0\u1ee3c g\u1eafn v\u00e0o ch\u00e2n \u0111\u1ebf nam ch\u00e2m (magnetic stand), l\u00fac n\u00e0y c\u00e1c h\u1ea1t t\u1eeb g\u1eafn DNA\/RNA s\u1ebd \u0111\u01b0\u1ee3c c\u00f4 l\u1eadp ra th\u00e0nh c\u1ee5m tr\u00ean th\u00e0nh tube do t\u1eeb tr\u01b0\u1eddng t\u00e1c \u0111\u1ed9ng b\u00ean ngo\u00e0i. <\/p>\n\n\n\n

Dung d\u1ecbch ch\u1ee9a c\u00e1c t\u1ea1p ch\u1ea5t s\u1ebd \u0111\u01b0\u1ee3c h\u00fat b\u1ecf ra b\u00ean ngo\u00e0i. \u1ede b\u01b0\u1edbc ti\u1ebfp theo, c\u00e1c dung d\u1ecbch r\u1eeda \u0111\u01b0\u1ee3c cho v\u00e0o tube \u0111\u1ec3 lo\u1ea1i b\u1ecf t\u1ea1p ch\u1ea5t, kh\u1ed1i h\u1ea1t t\u1eeb – nucleic acid l\u1ea7n l\u01b0\u1ee3t \u0111\u01b0\u1ee3c th\u1ea3 v\u00e0 h\u00fat l\u1ea1i b\u1eb1ng c\u00e1ch g\u1eafn v\u00e0o ch\u00e2n \u0111\u1ebf nam ch\u00e2m. Cu\u1ed1i c\u00f9ng dung d\u1ecbch \u0111\u1ec7m \u0111\u01b0\u1ee3c cho v\u00e0o gi\u00fap gi\u1ea3i ph\u00f3ng li\u00ean k\u1ebft gi\u1eefa h\u1ea1t t\u1eeb v\u00e0 nucleic acid, DNA\/RNA \u0111\u01b0\u1ee3c thu nh\u1eadn v\u00e0o tube m\u1edbi, h\u1ea1t t\u1eeb \u0111\u01b0\u1ee3c gom l\u1ea1i v\u00e0 lo\u1ea1i b\u1ecf. <\/p>\n\n\n\n

Nh\u00ecn chung, quy tr\u00ecnh th\u1ee7 c\u00f4ng th\u1ef1c hi\u1ec7n kh\u00e1 \u0111\u01a1n gi\u1ea3n, ng\u01b0\u1eddi th\u1ef1c hi\u1ec7n ch\u1ec9 c\u1ea7n th\u00eam h\u1ea1t t\u1eeb v\u00e0o m\u1eabu, sau \u0111\u00f3 \u0111\u1eb7t tube m\u1eabu l\u00ean ch\u00e2n \u0111\u1ebf nam ch\u00e2m \u0111\u1ec3 t\u00e1ch h\u1ea1t t\u1eeb d\u01b0\u1edbi s\u1ef1 t\u00e1c \u0111\u1ed9ng c\u1ee7a t\u1eeb tr\u01b0\u1eddng, h\u1ea1n ch\u1ebf \u00e1p l\u1ef1c m\u1eabu qua m\u00e0ng l\u1ecdc g\u00e2y t\u1eafc ngh\u1ebdn. Nh\u1edd v\u1eady ph\u01b0\u01a1ng ph\u00e1p n\u00e0y kh\u00f4ng c\u1ea7n l\u1ef1c ly t\u00e2m h\u1ed7 tr\u1ee3 n\u00ean c\u00f3 th\u1ec3 gi\u1ea3m \u0111\u01b0\u1ee3c chi ph\u00ed cho qu\u00e1 tr\u00ecnh t\u00e1ch chi\u1ebft. B\u00ean c\u1ea1nh \u0111\u00f3, ph\u01b0\u01a1ng ph\u00e1p n\u00e0y hi\u1ec7n nay c\u00f2n \u0111\u01b0\u1ee3c m\u1edf r\u1ed9ng \u1ee9ng d\u1ee5ng v\u00e0o c\u00e1c b\u1ed9 x\u00e9t nghi\u1ec7m di \u0111\u1ed9ng ph\u00f9 h\u1ee3p v\u1edbi m\u00f4i tr\u01b0\u1eddng th\u1ef1c \u0111\u1ecba, kh\u00f4ng y\u00eau c\u1ea7u cao v\u1ec1 \u0111i\u1ec1u ki\u1ec7n th\u00ed nghi\u1ec7m. \u0110i\u1ec1u l\u01b0u \u00fd do \u0111\u00e2y l\u00e0 ph\u01b0\u01a1ng ph\u00e1p th\u1ee7 c\u00f4ng n\u00ean ch\u1ea5t l\u01b0\u1ee3ng th\u00ed nghi\u1ec7m ph\u1ee5 thu\u1ed9c kh\u00e1 nhi\u1ec1u v\u00e0o k\u1ef9 thu\u1eadt c\u1ee7a ng\u01b0\u1eddi th\u1ef1c hi\u1ec7n, \u0111\u1eb7c bi\u1ec7t l\u00e0 thao t\u00e1c h\u00fat nh\u1ea3 h\u00f3a ch\u1ea5t b\u1eb1ng micropipette.<\/p>\n\n\n\n

\"H\u00ecnh
Figure 3.<\/strong> Quy tr\u00ecnh t\u00e1ch chi\u1ebft nucleic acid b\u1eb1ng h\u1ea1t t\u1eeb th\u1ee7 c\u00f4ng<\/em><\/figcaption><\/figure>\n\n\n\n

4. Ph\u01b0\u01a1ng ph\u00e1p t\u00e1ch chi\u1ebft h\u1ea1t t\u1eeb t\u1ef1 \u0111\u1ed9ng<\/strong><\/h2>\n\n\n\n

V\u1ec1 nguy\u00ean t\u1eafc ph\u01b0\u01a1ng ph\u00e1p t\u1ef1 \u0111\u1ed9ng c\u0169ng gi\u1ed1ng v\u1edbi th\u1ee7 c\u00f4ng, \u0111i\u1ec3m kh\u00e1c bi\u1ec7t ch\u00ednh \u0111\u00f3 l\u00e0 c\u00e1c b\u01b0\u1edbc th\u1ef1c hi\u1ec7n ph\u1ea7n l\u1edbn \u0111\u01b0\u1ee3c t\u1ef1 \u0111\u1ed9ng h\u00f3a ho\u00e0n to\u00e0n. Quy tr\u00ecnh s\u1eed d\u1ee5ng h\u1ec7 th\u1ed1ng t\u00e1ch chi\u1ebft t\u1ef1 \u0111\u1ed9ng th\u01b0\u1eddng bao g\u1ed3m ba b\u01b0\u1edbc ch\u00ednh: Cho m\u1eabu v\u00e0 h\u00f3a ch\u1ea5t v\u00e0o tube\/\u0111\u0129a tr\u01b0\u1edbc khi b\u1eaft \u0111\u1ea7u, sau \u0111\u00f3 c\u00e0i \u0111\u1eb7t ch\u01b0\u01a1ng tr\u00ecnh v\u00e0 v\u1eadn h\u00e0nh thi\u1ebft b\u1ecb, cu\u1ed1i c\u00f9ng thu nh\u1eadn nucleic acid (H\u00ecnh 4). Trong t\u00e1ch chi\u1ebft t\u1eeb th\u1ee7 c\u00f4ng, h\u1ea1t t\u1eeb \u1edf d\u1ea1ng \u201cb\u1ecb \u0111\u1ed9ng\u201d ch\u1ecbu t\u00e1c d\u1ee5ng c\u1ee7a l\u1ef1c t\u1eeb tr\u01b0\u1eddng b\u00ean ngo\u00e0i, trong khi \u0111\u00f3 v\u1edbi c\u00e1c thi\u1ebft b\u1ecb t\u1ef1 \u0111\u1ed9ng d\u00f9ng c\u00e1nh tay robot \u0111i\u1ec1u khi\u1ec3n c\u00e1c thanh t\u1eeb (vai tr\u00f2 gi\u1ed1ng nam ch\u00e2m) h\u00fat\/nh\u1ea3 h\u1ea1t t\u1eeb khi c\u1ea7n thi\u1ebft, nh\u01b0 v\u1eady h\u1ea1t t\u1eeb s\u1ebd l\u1ea7n l\u01b0\u1ee3t di chuy\u1ec3n m\u1ed9t c\u00e1ch \u201cch\u1ee7 \u0111\u1ed9ng\u201d qua c\u00e1c b\u01b0\u1edbc t\u00e1ch chi\u1ebft theo ch\u01b0\u01a1ng tr\u00ecnh c\u00e0i s\u1eb5n. \u0110i\u1ec3m kh\u00e1c bi\u1ec7t n\u00e0y gi\u00fap gia t\u0103ng hi\u1ec7u qu\u1ea3 tinh s\u1ea1ch nucleic acid c\u0169ng nh\u01b0 gi\u1ea3m s\u1ef1 can thi\u1ec7p c\u1ee7a con ng\u01b0\u1eddi v\u00e0 cung c\u1ea5p \u0111\u1ea7u ra ch\u1ea5t l\u01b0\u1ee3ng cao cho c\u00e1c th\u1eed nghi\u1ec7m ti\u1ebfp theo.<\/p>\n\n\n\n

Ngo\u00e0i ra, vi\u1ec7c th\u1ef1c hi\u1ec7n ho\u00e0n to\u00e0n trong bu\u1ed3ng m\u00e1y c\u00f3 k\u00e8m theo h\u1ec7 th\u1ed1ng UV kh\u1eed tr\u00f9ng gi\u00fap gi\u1ea3m thi\u1ec3u l\u00e2y nhi\u1ec5m ch\u00e9o. C\u00e1c h\u1ec7 th\u1ed1ng t\u1ef1 \u0111\u1ed9ng n\u00e0y c\u00f2n gi\u00fap d\u1ec5 d\u00e0ng l\u1eb7p l\u1ea1i th\u00ed nghi\u1ec7m v\u1edbi c\u00e1c ch\u01b0\u01a1ng tr\u00ecnh \u0111\u00e3 \u0111\u01b0\u1ee3c l\u01b0u l\u1ea1i, ki\u1ec3m so\u00e1t t\u1ed1t h\u01a1n c\u00e1c th\u00f4ng s\u1ed1 th\u00ed nghi\u1ec7m gi\u00fap t\u1ed1i \u01b0u h\u00f3a quy tr\u00ecnh t\u00e1ch chi\u1ebft. So v\u1edbi c\u00e1c ph\u01b0\u01a1ng ph\u00e1p th\u1ee7 c\u00f4ng truy\u1ec1n th\u1ed1ng, c\u00e1c h\u1ec7 th\u1ed1ng t\u00e1ch chi\u1ebft t\u1ef1 \u0111\u1ed9ng mang l\u1ea1i kh\u1ea3 n\u0103ng x\u1eed l\u00fd s\u1ed1 l\u01b0\u1ee3ng l\u1edbn m\u1eabu m\u1ed9t c\u00e1ch nhanh ch\u00f3ng, ti\u1ebft ki\u1ec7m th\u1eddi gian v\u00e0 c\u00f4ng s\u1ee9c cho nh\u00e2n vi\u00ean ph\u00f2ng th\u00ed nghi\u1ec7m m\u00e0 c\u00f2n n\u00e2ng cao \u0111\u1ed9 ch\u00ednh x\u00e1c c\u1ee7a k\u1ebft qu\u1ea3 ph\u00e2n t\u00edch.<\/p>\n\n\n\n

\"H\u00ecnh
Figure 4. <\/strong>Quy tr\u00ecnh t\u00e1ch chi\u1ebft nucleic acid b\u1eb1ng h\u1ea1t t\u1eeb t\u1ef1 \u0111\u1ed9ng<\/em><\/figcaption><\/figure>\n\n\n\n

Nh\u01b0 v\u1eady ph\u01b0\u01a1ng ph\u00e1p t\u00e1ch chi\u1ebft nucleic acid s\u1eed d\u1ee5ng h\u1ea1t t\u1eeb t\u00ednh \u0111\u00e3 c\u00f3 m\u1ed9t h\u00e0nh tr\u00ecnh ph\u00e1t tri\u1ec3n \u0111\u00e1ng k\u1ec3 trong l\u0129nh v\u1ef1c nghi\u00ean c\u1ee9u v\u00e0 \u1ee9ng d\u1ee5ng sinh h\u1ecdc ph\u00e2n t\u1eed. T\u1eeb vi\u1ec7c th\u1ef1c hi\u1ec7n m\u1ed7i b\u01b0\u1edbc c\u00f4ng vi\u1ec7c b\u1eb1ng tay \u0111\u1ebfn vi\u1ec7c s\u1eed d\u1ee5ng c\u00e1c h\u1ec7 th\u1ed1ng t\u1ef1 \u0111\u1ed9ng hi\u1ec7n \u0111\u1ea1i. M\u1ed7i ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ec1u c\u00f3 \u01b0u v\u00e0 nh\u01b0\u1ee3c \u0111i\u1ec3m ri\u00eang, t\u00f9y v\u00e0o nhu c\u1ea7u v\u00e0 \u0111i\u1ec1u ki\u1ec7n th\u00ed nghi\u1ec7m \u0111\u1ec3 c\u00f3 nh\u1eefng l\u1ef1a ch\u1ecdn ph\u00f9 h\u1ee3p trong qu\u00e1 tr\u00ecnh t\u00e1ch chi\u1ebft nucleic acid. B\u1ea3ng 1<\/em><\/strong> t\u00f3m g\u1ecdn l\u1ea1i c\u00e1c \u01b0u nh\u01b0\u1ee3c \u0111i\u1ec3m c\u1ee7a c\u1ea3 2 ph\u01b0\u01a1ng ph\u00e1p t\u00e1ch chi\u1ebft b\u1eb1ng h\u1ea1t t\u1eeb t\u00ednh th\u1ee7 c\u00f4ng v\u00e0 t\u1ef1 \u0111\u1ed9ng.<\/p>\n\n\n\n

B\u1ea3ng 1. <\/strong>So s\u00e1nh \u01b0u v\u00e0 nh\u01b0\u1ee3c \u0111i\u1ec3m c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p t\u00e1ch chi\u1ebft b\u1eb1ng h\u1ea1t t\u1eeb t\u00ednh th\u1ee7 c\u00f4ng v\u00e0 t\u1ef1 \u0111\u1ed9ng<\/em><\/p>\n\n\n\n

Ph\u01b0\u01a1ng ph\u00e1p t\u00e1ch chi\u1ebft h\u1ea1t t\u1eeb t\u00ednh<\/strong><\/td>Th\u1ee7 c\u00f4ng<\/strong><\/td>T\u1ef1 \u0111\u1ed9ng<\/strong><\/td><\/tr>
Highlights<\/strong> <\/td>– Y\u00eau c\u1ea7u d\u1ee5ng c\u1ee5 \u0111\u01a1n gi\u1ea3n, kh\u00f4ng c\u1ea7n h\u1ec7 th\u1ed1ng m\u00e1y ly t\u00e2m
– Chi ph\u00ed th\u1ea5p
– C\u00f3 th\u1ec3 \u1ee9ng d\u1ee5ng trong x\u00e9t nghi\u1ec7m di \u0111\u1ed9ng ngo\u00e0i th\u1ef1c \u0111\u1ecba<\/td>		– \u0110\u01a1n gi\u1ea3n, d\u1ec5 th\u1ef1c hi\u1ec7n, kh\u00f4ng y\u00eau c\u1ea7u thao t\u00e1c tay nhi\u1ec1u
– X\u1eed l\u00fd \u0111\u1ed3ng th\u1eddi s\u1ed1 l\u01b0\u1ee3ng m\u1eabu l\u1edbn, gi\u00fap ti\u1ebft ki\u1ec7m th\u1eddi gian
– H\u1ea1n ch\u1ebf nhi\u1ec5m ch\u00e9o
– Ki\u1ec3m so\u00e1t c\u00e1c th\u00f4ng s\u1ed1 d\u1ec5 d\u00e0ng
– D\u1ec5 d\u00e0ng t\u1ed1i \u01b0u h\u00f3a quy tr\u00ecnh<\/td><\/tr>
Nh\u01b0\u1ee3c \u0111i\u1ec3m<\/td>– Y\u00eau c\u1ea7u thao t\u00e1c k\u1ef9 thu\u1eadt vi\u00ean t\u1ed1t
– H\u1ea1n ch\u1ebf khi x\u1eed l\u00fd s\u1ed1 l\u01b0\u1ee3ng m\u1eabu nhi\u1ec1u<\/td>		– Chi ph\u00ed cao<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

5. T\u00e0i li\u1ec7u tham kh\u1ea3o<\/strong><\/h2>\n\n\n\n

1. Li, Y., Liu, S., Wang, Y., Wang, Y., Li, S., He, N., … & Chen, Z. (2023). Research on a Magnetic Separation-Based Rapid Nucleic Acid Extraction System and Its Detection Applications.<\/a> Biosensors<\/em>, 13<\/em>(10), 903.<\/p>\n\n\n\n

2. L\u01b0\u01a1ng Hu\u1ef3nh V\u1ee7 Thanh, Th\u1ea1ch Tr\u1ea7n Ph\u01b0\u01a1ng Anh, Ng\u00f4 Tu\u1ea5n Ki\u1ec7t v\u00e0 L\u00fd \u0110\u1ee9c (2021). T\u1ed5ng h\u1ee3p v\u1eadt li\u1ec7u nano Fe3O4@ SiO2 c\u1ea5u tr\u00fac l\u00f5i v\u1ecf c\u00f3 \u0111\u1ed9 t\u1eeb h\u00f3a cao<\/a>. T\u1ea1p ch\u00ed Khoa h\u1ecdc \u0110\u1ea1i h\u1ecdc c\u1ea7n Th\u01a1<\/em>, 57<\/em>(3), 53-64.<\/p>\n\n\n\n

<\/p>","protected":false},"excerpt":{"rendered":"

M\u1ed9t quy tr\u00ecnh ch\u1ea9n \u0111o\u00e1n ph\u00e2n t\u1eed ho\u00e0n ch\u1ec9nh s\u1ebd bao g\u1ed3m thu m\u1eabu, x\u1eed l\u00fd m\u1eabu, t\u00e1ch chi\u1ebft nucleic acid, ph\u00e2n t\u00edch ph\u00e2n t\u1eed v\u00e0 \u0111\u00e1nh gi\u00e1, di\u1ec5n gi\u1ea3i k\u1ebft qu\u1ea3. Trong \u0111\u00f3, b\u01b0\u1edbc t\u00e1ch chi\u1ebft s\u1ebd ph\u00e2n l\u1eadp v\u00e0 l\u00e0m s\u1ea1ch ph\u00e2n t\u1eed nucleic acid m\u1ee5c ti\u00eau. \u0110\u00e2y \u0111\u01b0\u1ee3c coi l\u00e0 b\u01b0\u1edbc kh\u00f4ng th\u1ec3 thi\u1ebfu trong x\u00e9t nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed.<\/p>","protected":false},"author":1,"featured_media":10792,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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