{"id":10314,"date":"2024-04-08T11:56:28","date_gmt":"2024-04-08T04:26:28","guid":{"rendered":"https:\/\/abtvn.com\/?p=10314"},"modified":"2025-08-06T14:44:20","modified_gmt":"2025-08-06T07:14:20","slug":"tieu-chuan-phong-thi-nghiem-sinh-hoc-phan-tu","status":"publish","type":"post","link":"https:\/\/abtvn.com\/en\/tieu-chuan-phong-thi-nghiem-sinh-hoc-phan-tu\/","title":{"rendered":"Ti\u00eau chu\u1ea9n ph\u00f2ng th\u00ed nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed<\/strong>"},"content":{"rendered":"

1. X\u00e9t nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed v\u00e0 ph\u00f2ng th\u00ed nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed<\/strong><\/h2>\n\n\n\n

Sinh h\u1ecdc ph\u00e2n t\u1eed l\u00e0 m\u1ed9t l\u0129nh v\u1ef1c khoa h\u1ecdc ti\u00ean ti\u1ebfn, s\u1eed d\u1ee5ng c\u00e1c k\u1ef9 thu\u1eadt ph\u00e2n t\u00edch tr\u00ean c\u00e1c ch\u1ec9 th\u1ecb sinh h\u1ecdc \u1edf m\u1ee9c \u0111\u1ed9 ph\u00e2n t\u1eed nh\u01b0 DNA, RNA v\u00e0 protein. C\u00e1c ph\u01b0\u01a1ng ph\u00e1p n\u00e0y kh\u00f4ng ch\u1ec9 gi\u00fap \u0111\u00e1nh gi\u00e1 ch\u1ee9c n\u0103ng, m\u1ee9c \u0111\u1ed9 bi\u1ec3u hi\u1ec7n v\u00e0 c\u00e1c bi\u1ebfn \u0111\u1ed5i gen m\u00e0 c\u00f2n \u0111\u1eb7c bi\u1ec7t hi\u1ec7u qu\u1ea3 trong vi\u1ec7c ph\u00e1t hi\u1ec7n s\u1ef1 t\u1ed3n t\u1ea1i c\u1ee7a c\u00e1c ch\u1ec9 th\u1ecb sinh h\u1ecdc m\u1ee5c ti\u00eau li\u00ean quan \u0111\u1ebfn c\u00e1c b\u1ec7nh l\u00fd v\u00e0 t\u00ecnh tr\u1ea1ng s\u1ee9c kh\u1ecfe. Ph\u00f2ng th\u00ed nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed \u0111\u00f3ng vai tr\u00f2 then ch\u1ed1t trong vi\u1ec7c th\u1ef1c hi\u1ec7n c\u00e1c x\u00e9t nghi\u1ec7m n\u00e0y, t\u1eeb ch\u1ea9n \u0111o\u00e1n b\u1ec7nh, theo d\u00f5i qu\u00e1 tr\u00ecnh \u0111i\u1ec1u tr\u1ecb \u0111\u1ebfn nghi\u00ean c\u1ee9u gen v\u00e0 c\u00e1c ch\u1ec9 th\u1ecb sinh h\u1ecdc kh\u00e1c.<\/p>\n\n\n\n

C\u00e1c k\u1ef9 thu\u1eadt ph\u1ed5 bi\u1ebfn nh\u01b0 PCR<\/a><\/strong>, Real-time PCR<\/a><\/strong>, reverse dot blot<\/a><\/strong>, western blot, northern blot, southern blot v\u00e0 gi\u1ea3i tr\u00ecnh t\u1ef1 \u0111\u1ec1u \u0111\u01b0\u1ee3c tri\u1ec3n khai trong c\u00e1c ph\u00f2ng th\u00ed nghi\u1ec7m n\u00e0y \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o \u0111\u1ed9 nh\u1ea1y v\u00e0 \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u cao. \u0110\u1eb7c bi\u1ec7t, PCR v\u00e0 Real-time PCR<\/strong><\/a> l\u00e0 hai k\u1ef9 thu\u1eadt ch\u00ednh trong x\u00e9t nghi\u1ec7m l\u00e2m s\u00e0ng \u1ee9ng d\u1ee5ng sinh h\u1ecdc ph\u00e2n t\u1eed, mang l\u1ea1i k\u1ebft qu\u1ea3 c\u00f3 \u0111\u1ed9 ch\u00ednh x\u00e1c cao.<\/p>\n\n\n\n

V\u00ec v\u1eady, ph\u00f2ng th\u00ed nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed, c\u00e1c trung t\u00e2m x\u00e9t nghi\u1ec7m ki\u1ec3m chu\u1ea9n, ph\u00f2ng x\u00e9t nghi\u1ec7m m\u1eabu b\u1ec7nh tr\u00ean th\u00fa y, ng\u01b0\u1eddi, th\u1ee7y s\u1ea3n, th\u1ef1c ph\u1ea9m c\u1ea7n \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf v\u00e0 x\u00e2y d\u1ef1ng \u0111\u1ea1t chu\u1ea9n nh\u1eb1m \u0111\u1ea3m b\u1ea3o kh\u00f4ng gian ph\u00f9 h\u1ee3p nh\u1ea5t \u0111\u1ec3 th\u1ef1c hi\u1ec7n c\u00e1c x\u00e9t nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed v\u00e0 tu\u00e2n theo c\u00e1c Ngh\u1ecb \u0111\u1ecbnh c\u1ee7a Ch\u00ednh Ph\u1ee7 v\u00e0 Th\u00f4ng t\u01b0 c\u1ee7a B\u1ed9 Y t\u1ebf. Ngo\u00e0i ra ch\u00fang c\u1ea7n \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf, trang b\u1ecb c\u00e1c thi\u1ebft b\u1ecb v\u00e0 m\u00e1y m\u00f3c c\u1ea7n thi\u1ebft cho c\u00e1c k\u1ef9 thu\u1eadt sinh h\u1ecdc ph\u00e2n t\u1eed theo c\u00e1c h\u01b0\u1edbng d\u1eabn t\u1eeb T\u1ed5 ch\u1ee9c Y t\u1ebf Th\u1ebf gi\u1edbi \u2013 WHO.<\/p>\n\n\n\n

<\/div>\n\n\n\n

2. C\u1ea5p \u0111\u1ed9 v\u00e0 ti\u00eau chu\u1ea9n ph\u00f2ng th\u00ed nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed<\/strong><\/h2>\n\n\n\n

Ph\u00f2ng th\u00ed nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed \u0111\u01b0\u1ee3c x\u00e2y d\u1ef1ng \u0111\u1ec3 thao t\u00e1c tr\u00ean c\u00e1c vi sinh v\u1eadt g\u00e2y b\u1ec7nh, v\u00ec v\u1eady ph\u00f2ng c\u1ea7n \u0111\u1ea1t \u0111\u01b0\u1ee3c c\u00e1c c\u1ea5p \u0111\u1ed9 an to\u00e0n sinh h\u1ecdc ph\u00f9 h\u1ee3p t\u00f9y v\u00e0o \u0111\u1ed1i t\u01b0\u1ee3ng vi sinh v\u1eadt th\u1ef1c hi\u1ec7n.<\/p>\n\n\n\n

Theo ngh\u1ecb \u0111\u1ecbnh 103 c\u1ee7a Ch\u00ednh Ph\u1ee7 (103\/2016\/N\u0110-CP), c\u00e1c vi sinh v\u1eadt c\u00f3 nguy c\u01a1 g\u00e2y b\u1ec7nh truy\u1ec1n nhi\u1ec5m cho ng\u01b0\u1eddi \u0111\u01b0\u1ee3c chia th\u00e0nh 4 nh\u00f3m (nh\u00f3m 1, nh\u00f3m 2, nh\u00f3m 3 v\u00e0 nh\u00f3m 4) t\u00f9y theo nguy c\u01a1 l\u00e2y nhi\u1ec5m cho c\u00e1 th\u1ec3 v\u00e0 c\u1ed9ng \u0111\u1ed3ng, kh\u1ea3 n\u0103ng g\u00e2y b\u1ec7nh, c\u00f3 hay kh\u00f4ng bi\u1ec7n ph\u00e1p ph\u00f2ng ch\u1ed1ng l\u00e2y nhi\u1ec5m v\u00e0 \u0111i\u1ec1u tr\u1ecb.<\/p>\n\n\n\n

T\u1eeb \u0111\u00f3 c\u01a1 s\u1edf x\u00e9t nghi\u1ec7m \u0111\u01b0\u1ee3c ph\u00e2n lo\u1ea1i theo 4 c\u1ea5p \u0111\u1ed9 an to\u00e0n sinh h\u1ecdc (c\u1ea5p 1, c\u1ea5p 2, c\u1ea5p 3 v\u00e0 c\u1ea5p 4) ph\u1ee5 thu\u1ed9c v\u00e0o nh\u00f3m vi sinh v\u1eadt th\u1ef1c hi\u1ec7n. Trong \u0111\u00f3 c\u00e1c t\u00e1c nh\u00e2n g\u00e2y b\u1ec7nh (virus, vi khu\u1ea9n, k\u00ed sinh tr\u00f9ng,\u2026) c\u1ea7n x\u00e9t nghi\u1ec7m ch\u1ee7 y\u1ebfu thu\u1ed9c nh\u00f3m nguy c\u01a1 2 theo ban h\u00e0nh danh m\u1ee5c vi sinh v\u1eadt g\u00e2y b\u1ec7nh truy\u1ec1n nhi\u1ec5m \u0111\u01b0\u1ee3c quy \u0111\u1ecbnh trong th\u00f4ng t\u01b0 s\u1ed1 41\/2016\/TT-BYT<\/a>. Ch\u00ednh v\u00ec v\u1eady ph\u00f2ng th\u00ed nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed ch\u1ee7 y\u1ebfu \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf \u0111\u1ec3 \u0111\u1ea1t c\u01a1 s\u1edf x\u00e9t nghi\u1ec7m an to\u00e0n sinh h\u1ecdc c\u1ea5p 2<\/strong> v\u00e0 th\u1ef1c hi\u1ec7n tr\u00ean c\u00e1c \u0111\u1ed1i t\u01b0\u1ee3ng g\u1ed3m c\u00e1c vi sinh v\u1eadt thu\u1ed9c nh\u00f3m 1 v\u00e0 nh\u00f3m 2 \u0111\u01b0\u1ee3c quy \u0111\u1ecbnh trong th\u00f4ng t\u01b0 s\u1ed1 41\/2016\/TT-BYT<\/p>\n\n\n\n

<\/div>\n\n\n\n

C\u01a1 s\u1edf x\u00e9t nghi\u1ec7m an to\u00e0n sinh h\u1ecdc c\u1ea5p II ph\u1ea3i \u0111\u00e1p \u1ee9ng c\u00e1c ti\u00eau chu\u1ea9n sau:<\/em><\/strong><\/p>\n\n\n\n

\u25ba Ti\u00eau chu\u1ea9n v\u1ec1 c\u01a1 s\u1edf v\u1eadt ch\u1ea5t:<\/strong><\/h3>\n\n\n\n
    \n
  1. C\u00e1c ti\u00eau chu\u1ea9n v\u1ec1 s\u00e0n; t\u01b0\u1eddng; b\u00e0n x\u00e9t nghi\u1ec7m; b\u1ed3n n\u01b0\u1edbc r\u1eeda tay; d\u1ee5ng c\u1ee5 r\u1eeda m\u1eaft; h\u1ec7 th\u1ed1ng \u0111i\u1ec7n, n\u01b0\u1edbc, \u00e1nh s\u00e1ng; thi\u1ebft b\u1ecb ph\u00f2ng, ch\u1ed1ng ch\u00e1y n\u1ed5; h\u1ec7 th\u1ed1ng thu gom, x\u1eed l\u00fd ho\u1eb7c trang thi\u1ebft b\u1ecb x\u1eed l\u00fd n\u01b0\u1edbc th\u1ea3i \u0111\u01b0\u1ee3c quy \u0111\u1ecbnh trong \u0111i\u1ec3m a, b, c, d, \u0111, e kho\u1ea3n 2 \u0111i\u1ec1u 5 c\u1ee7a Ngh\u1ecb \u0111\u1ecbnh 103\/2016\/N\u0110-CP<\/li>\n\n\n\n
  2. Ph\u1ea3i ri\u00eang bi\u1ec7t v\u1edbi c\u00e1c ph\u00f2ng kh\u00e1c c\u1ee7a c\u01a1 s\u1edf x\u00e9t nghi\u1ec7m;<\/li>\n\n\n\n
  3. C\u00f3 bi\u1ec3n b\u00e1o nguy hi\u1ec3m sinh h\u1ecdc tr\u00ean c\u1eeda ra v\u00e0o c\u1ee7a khu v\u1ef1c x\u00e9t nghi\u1ec7m<\/li>\n<\/ol>\n\n\n\n

    \u25ba Ti\u00eau chu\u1ea9n v\u1ec1 trang thi\u1ebft b\u1ecb:<\/strong><\/h3>\n\n\n\n
      \n
    1. C\u00e1c trang thi\u1ebft b\u1ecb c\u1ea7n thi\u1ebft \u0111\u01b0\u1ee3c quy \u0111\u1ecbnh trong \u0111i\u1ec3m a, b kho\u1ea3n 2 \u0111i\u1ec1u 5 c\u1ee7a Ngh\u1ecb \u0111\u1ecbnh 103\/2016\/N\u0110-CP<\/li>\n\n\n\n
    2. C\u00f3 t\u1ee7 an to\u00e0n sinh h\u1ecdc; thi\u1ebft b\u1ecb h\u1ea5p ch\u1ea5t th\u1ea3i y t\u1ebf l\u00e2y nhi\u1ec5m ho\u1eb7c thi\u1ebft b\u1ecb kh\u1eed khu\u1ea9n; \u0111\u1ea7u t\u01b0 c\u00e1c trang thi\u1ebft b\u1ecb b\u1ea3o h\u1ed9 c\u00e1 nh\u00e2n ph\u00f9 h\u1ee3p v\u1edbi lo\u1ea1i k\u1ef9 thu\u1eadt x\u00e9t nghi\u1ec7m.<\/li>\n<\/ol>\n\n\n\n

      \u25ba Ti\u00eau chu\u1ea9n v\u1ec1 nh\u00e2n s\u1ef1:<\/strong><\/h3>\n\n\n\n
        \n
      1. C\u00f3 \u00edt nh\u1ea5t 02 nh\u00e2n vi\u00ean x\u00e9t nghi\u1ec7m. Nh\u00e2n vi\u00ean tr\u1ef1c ti\u1ebfp th\u1ef1c hi\u1ec7n ph\u1ea3i c\u00f3 v\u0103n b\u1eb1ng, ch\u1ee9ng ch\u1ec9 \u0111\u00e0o t\u1ea1o ph\u00f9 h\u1ee3p v\u1edbi lo\u1ea1i h\u00ecnh x\u00e9t nghi\u1ec7m.<\/li>\n\n\n\n
      2. Nh\u00e2n vi\u00ean ph\u00f2ng th\u00ed nghi\u1ec7m, ng\u01b0\u1eddi ch\u1ecbu tr\u00e1ch nhi\u1ec7m v\u1ec1 an to\u00e0n sinh h\u1ecdc ph\u1ea3i \u0111\u01b0\u1ee3c t\u1eadp hu\u1ea5n v\u1ec1 an to\u00e0n sinh h\u1ecdc t\u1eeb c\u1ea5p II tr\u1edf l\u00ean v\u00e0 nh\u1eefng ng\u01b0\u1eddi kh\u00e1c l\u00e0m vi\u1ec7c trong khu v\u1ef1c x\u00e9t nghi\u1ec7m ph\u1ea3i \u0111\u01b0\u1ee3c h\u01b0\u1edbng d\u1eabn v\u1ec1 an to\u00e0n sinh h\u1ecdc ph\u00f9 h\u1ee3p v\u1edbi c\u00f4ng vi\u1ec7c.<\/li>\n<\/ol>\n\n\n\n

        \u25ba Ti\u00eau chu\u1ea9n v\u1ec1 quy \u0111\u1ecbnh th\u1ef1c h\u00e0nh:<\/strong><\/h3>\n\n\n\n
          \n
        1. C\u00f3 quy \u0111\u1ecbnh ra v\u00e0o khu v\u1ef1c x\u00e9t nghi\u1ec7m; quy \u0111\u1ecbnh ch\u1ebf \u0111\u1ed9 b\u00e1o c\u00e1o; quy tr\u00ecnh l\u01b0u tr\u1eef h\u1ed3 s\u01a1; quy tr\u00ecnh x\u00e9t nghi\u1ec7m; h\u01b0\u1edbng d\u1eabn s\u1eed d\u1ee5ng c\u00e1c trang thi\u1ebft b\u1ecb; quy tr\u00ecnh v\u1ec1 kh\u1eed nhi\u1ec5m v\u00e0 x\u1eed l\u00fd ch\u1ea5t th\u1ea3i; quy \u0111\u1ecbnh gi\u00e1m s\u00e1t s\u1ee9c kh\u1ecfe v\u00e0 y t\u1ebf; quy \u0111\u1ecbnh l\u01b0u gi\u1eef, b\u1ea3o qu\u1ea3n m\u1eabu b\u1ec7nh ph\u1ea9m, t\u00e1c nh\u00e2n g\u00e2y b\u1ec7nh truy\u1ec1n nhi\u1ec5m.<\/li>\n\n\n\n
        2. C\u00f3 k\u1ebf ho\u1ea1ch \u0111\u00e0o t\u1ea1o, t\u1eadp hu\u1ea5n nh\u00e2n vi\u00ean l\u00e0m vi\u1ec7c t\u1ea1i khu v\u1ef1c x\u00e9t nghi\u1ec7m;<\/li>\n\n\n\n
        3. C\u00f3 k\u1ebf ho\u1ea1ch \u0111\u00e1nh gi\u00e1 nguy c\u01a1 x\u1ea3y ra s\u1ef1 c\u1ed1 an to\u00e0n sinh h\u1ecdc t\u1ea1i c\u01a1 s\u1edf x\u00e9t nghi\u1ec7m v\u00e0 x\u00e2y d\u1ef1ng k\u1ebf ho\u1ea1ch ph\u00f2ng ng\u1eeba, x\u1eed l\u00fd s\u1ef1 c\u1ed1 an to\u00e0n sinh h\u1ecdc.<\/li>\n<\/ol>\n\n\n\n
          <\/div>\n\n\n\n

          3. Thi\u1ebft k\u1ebf v\u00e0 trang thi\u1ebft b\u1ecb ph\u00f2ng th\u00ed nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed<\/strong><\/h2>\n\n\n\n

          Theo h\u01b0\u1edbng d\u1eabn t\u1eeb WHO, m\u1ed9t ph\u00f2ng th\u00ed nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed d\u00f9ng cho x\u00e9t nghi\u1ec7m ch\u1ea9n \u0111o\u00e1n b\u1eb1ng k\u1ef9 thu\u1eadt Real-time PCR \u0111\u01b0\u1ee3c chia th\u00e0nh \u00edt nh\u1ea5t ba ph\u00f2ng ri\u00eang bi\u1ec7t (H\u00ecnh 1)<\/em><\/strong><\/p>\n\n\n\n

          \"Ti\u00eau
          Figure 1 <\/strong>S\u01a1 \u0111\u1ed3 ph\u00f2ng x\u00e9t nghi\u1ec7m PCR theo khuy\u1ebfn c\u00e1o c\u1ee7a WHO<\/em><\/figcaption><\/figure>\n\n\n\n

          \u25ba Ph\u00f2ng chu\u1ea9n b\u1ecb h\u00f3a ch\u1ea5t:<\/strong><\/p>\n\n\n\n

            \n
          • Ch\u1ee9c n\u0103ng: d\u00f9ng \u0111\u1ec3 b\u1ea3o qu\u1ea3n v\u00e0 pha h\u00f3a ch\u1ea5t cho ph\u1ea3n \u1ee9ng real-time PCR. \u0110\u00e2y l\u00e0 ph\u00f2ng n\u00ean thao t\u00e1c \u0111\u1ea7u ti\u00ean trong quy tr\u00ecnh th\u1ef1c hi\u1ec7n.<\/li>\n\n\n\n
          • Thi\u1ebft b\u1ecb d\u1ee5ng c\u1ee5 c\u1ea7n c\u00f3:<\/li>\n<\/ul>\n\n\n\n

            T\u1ee7 PCR<\/strong><\/em><\/p>\n\n\n\n

            \"\"<\/figure>\n\n\n\n

            T\u1ee7 \u0111\u00f4ng \u00e2m s\u00e2u \u0111\u1ec3 b\u1ea3o qu\u1ea3n h\u00f3a ch\u1ea5t (t\u1ed1i thi\u1ec3u -20 \u0111\u1ed9 C)<\/em><\/p>\n\n\n\n

            T\u1ee7 \u0111\u00f4ng \u00e2m s\u00e2u \u0111\u1ec3 b\u1ea3o qu\u1ea3n m\u1eabu (-20 \u0111\u1ed9 C) ho\u1eb7c t\u1ee7 m\u00e1t (2-8 \u0111\u1ed9 C)<\/em><\/strong><\/p>\n\n\n\n

            \"\"
            T\u1ee7 \u00e2m s\u00e2u -20 \u0111\u1ed9 C<\/em><\/figcaption><\/figure>\n\n\n\n

            M\u00e1y ly t\u00e2m mini (Spindown)<\/strong><\/em><\/p>\n\n\n\n

            \"\"
            M\u00e1y ly t\u00e2m mini (h\u00e3ng DLAB)<\/em><\/figcaption><\/figure>\n\n\n\n

            Micropipette<\/strong><\/em><\/p>\n\n\n\n

            \"\"
            Micropipette h\u00e3ng DLAB (ch\u1ee7 y\u1ebfu lo\u1ea1i 2-20uL)<\/em><\/figcaption><\/figure>\n\n\n\n

            \u25ba Ph\u00f2ng chu\u1ea9n b\u1ecb m\u1eabu<\/strong> (l\u00fd t\u01b0\u1edfng nh\u1ea5t l\u00e0 chia th\u00e0nh 2 ph\u00f2ng nh\u1ecf l\u00e0 ph\u00f2ng t\u00e1ch chi\u1ebft v\u00e0 ph\u00f2ng n\u1ea1p m\u1eabu ho\u1eb7c hai khu v\u1ef1c th\u1ef1c hi\u1ec7n ri\u00eang bi\u1ec7t):<\/p>\n\n\n\n

              \n
            • Ch\u1ee9c n\u0103ng: x\u1eed l\u00fd, t\u00e1ch chi\u1ebft v\u00e0 n\u1ea1p m\u1eabu DNA\/RNA sau t\u00e1ch chi\u1ebft v\u00e0o mix real-time PCR \u0111\u00e3 chu\u1ea9n b\u1ecb. Vi\u1ec7c ph\u00e2n chia nh\u01b0 v\u1eady s\u1ebd kh\u00f4ng l\u00e0m nhi\u1ec5m c\u00e1c t\u1ea1p ch\u1ea5t hay DNA\/RNA t\u1eeb t\u00e1c nh\u00e2n vi sinh v\u1eadt v\u00e0o b\u1ed9 h\u00f3a ch\u1ea5t real-time PCR (\u0111\u01b0\u1ee3c chu\u1ea9n b\u1ecb t\u1eeb ph\u00f2ng pha h\u00f3a ch\u1ea5t)<\/li>\n\n\n\n
            • Thi\u1ebft b\u1ecb d\u1ee5ng c\u1ee5 c\u1ea7n c\u00f3:<\/li>\n<\/ul>\n\n\n\n

              T\u1ee7 an to\u00e0n sinh h\u1ecdc c\u1ea5p 2 (d\u00f9ng cho qu\u00e1 tr\u00ecnh x\u1eed l\u00fd v\u00e0 t\u00e1ch chi\u1ebft m\u1eabu)<\/em><\/strong><\/p>\n\n\n\n

              \"\"
              T\u1ee7 an to\u00e0n sinh h\u1ecdc c\u1ea5p II (h\u00e3ng ABT)<\/em><\/figcaption><\/figure>\n\n\n\n

              T\u1ee7 PCR (d\u00f9ng cho qu\u00e1 tr\u00ecnh n\u1ea1p m\u1eabu DNA\/RNA sau t\u00e1ch chi\u1ebft v\u00e0o h\u00f3a ch\u1ea5t real-time PCR \u0111\u00e3 chu\u1ea9n b\u1ecb)<\/em><\/strong><\/p>\n\n\n\n

              \"\"
              T\u1ee7 PCR (h\u00e3ng ABT)<\/em><\/figcaption><\/figure>\n\n\n\n

              M\u00e1y t\u00e1ch chi\u1ebft acid nucleic t\u1ef1 \u0111\u1ed9ng (cho gi\u1ea3i ph\u00e1p t\u00e1ch chi\u1ebft t\u1ef1 \u0111\u1ed9ng)<\/strong><\/em><\/p>\n\n\n\n

              \"\"
              M\u00e1y t\u00e1ch chi\u1ebft t\u1ef1 \u0111\u1ed9ng (H\u00e3ng ABT \u2013 t\u1ed1i \u0111a 32 m\u1eabu 1 l\u1ea7n ch\u1ea1y)<\/em><\/figcaption><\/figure>\n\n\n\n

              M\u00e1y tr\u1ed9n m\u1eabu – vortex<\/em><\/strong>, M\u00e1y Spindown<\/em><\/strong><\/p>\n\n\n\n

              \"\"
              M\u00e1y tr\u1ed9n m\u1eabu \u2013 vortex (h\u00e3ng DLAB)<\/em><\/figcaption><\/figure>\n\n\n\n

              M\u00e1y \u1ee7 nhi\u1ec7t kh\u00f4 v\u00e0 m\u00e1y ly t\u00e2m t\u1ed1c \u0111\u1ed9 cao (cho gi\u1ea3i ph\u00e1p t\u00e1ch chi\u1ebft th\u1ee7 c\u00f4ng)<\/em><\/strong>, m\u00e1y ly t\u00e2m \u1ed1ng 15ml (d\u00f9ng ly t\u00e2m thu huy\u1ebft thanh \/ huy\u1ebft t\u01b0\u01a1ng)<\/em><\/strong><\/p>\n\n\n\n

              \"\"
              M\u00e1y \u1ee7 nhi\u1ec7t kh\u00f4, m\u00e1y ly t\u00e2m t\u1ed1c \u0111\u1ed9 cao, m\u00e1y ly t\u00e2m <\/em><\/figcaption><\/figure>\n\n\n\n
              <\/div>\n\n\n\n

              \u25ba Ph\u00f2ng Real-time PCR\/PCR:<\/strong><\/p>\n\n\n\n

                \n
              • Ch\u1ee9c n\u0103ng: s\u1eed d\u1ee5ng \u0111\u1ec3 ch\u1ea1y real-time PCR\/PCR v\u00e0 ph\u00e2n t\u00edch k\u1ebft qu\u1ea3.<\/li>\n\n\n\n
              • Thi\u1ebft b\u1ecb d\u1ee5ng c\u1ee5 c\u1ea7n c\u00f3:<\/li>\n<\/ul>\n\n\n\n

                M\u00e1y real-time PCR ho\u1eb7c PCR<\/em><\/strong><\/p>\n\n\n\n

                \"\"
                M\u00e1y real-time PCR (H\u00e3ng Bioer \u2013 l\u1ea7n l\u01b0\u1ee3t 16 48 v\u00e0 96 m\u1eabu m\u1ed9t l\u1ea7n ch\u1ea1y)<\/em><\/figcaption><\/figure>\n\n\n\n

                B\u1ed9 m\u00e1y t\u00ednh ph\u00e2n t\u00edch k\u1ebft qu\u1ea3 (m\u00e1y in n\u1ebfu c\u1ea7n)<\/em><\/strong><\/p>\n\n\n\n

                <\/div>\n\n\n\n

                M\u1ed7i khu v\u1ef1c l\u00e0m vi\u1ec7c c\u1ea7n \u0111\u01b0\u1ee3c b\u1ed1 tr\u00ed \u0111\u1ea7y \u0111\u1ee7 trang thi\u1ebft b\u1ecb, d\u1ee5ng c\u1ee5, b\u1ea3o h\u1ed9 c\u00e1 nh\u00e2n, kh\u00f4ng s\u1eed d\u1ee5ng l\u1eabn gi\u1eefa c\u00e1c khu v\u1ef1c. V\u1ec1 quy tr\u00ecnh th\u1ef1c hi\u1ec7n ph\u1ea3i theo quy t\u1eafc 1 chi\u1ec1u (quy tr\u00ecnh nh\u01b0 S\u01a1 \u0111\u1ed3 1<\/em><\/strong>). C\u00e1c ph\u00f2ng s\u1ebd th\u00f4ng v\u1edbi nhau qua passbox \u0111\u1ec3 di chuy\u1ec3n m\u1eabu th\u00ed nghi\u1ec7m \u0111i qua t\u1eebng ph\u00f2ng, tr\u00e1nh nhi\u1ec5m ch\u00e9o, \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn k\u1ebft qu\u1ea3 th\u00ed nghi\u1ec7m v\u00e0 nhi\u1ec5m ra khu v\u1ef1c h\u00e0nh lang ho\u1eb7c khu v\u1ef1c b\u00ean ngo\u00e0i. Ngo\u00e0i ra c\u1ea7n thi\u1ebft c\u00f3 th\u00eam ph\u00f2ng \u0111\u1ec7m nh\u1eb1m m\u1ee5c \u0111\u00edch l\u00e0 khu v\u1ef1c \u0111\u1ec3 c\u00e1c trang thi\u1ebft b\u1ecb b\u1ea3o h\u1ed9 nh\u01b0 \u00e1o blouse, b\u1ed3n r\u1eeda tay ho\u1eb7c l\u00e0 khu v\u1ef1c \u0111\u1ec3 di chuy\u1ec3n qua c\u00e1c ph\u00f2ng theo quy t\u1eafc m\u1ed9t chi\u1ec1u.<\/p>\n\n\n\n

                \"\"
                S\u01a1 \u0111\u1ed3 1: <\/strong>Quy t\u1eafc m\u1ed9t chi\u1ec1u trong quy tr\u00ecnh th\u1ef1c hi\u1ec7n real-time PCR trong ch\u1ea9n \u0111o\u00e1n t\u00e1c nh\u00e2n g\u00e2y b\u1ec7nh<\/em><\/figcaption><\/figure>\n\n\n\n

                Nh\u00ecn chung, vi\u1ec7c thi\u1ebft k\u1ebf ph\u00f2ng x\u00e9t nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed \u1ee9ng d\u1ee5ng c\u00e1c k\u1ef9 thu\u1eadt PCR\/qPCR s\u1ebd ph\u1ee5 thu\u1ed9c nhi\u1ec1u v\u00e0o di\u1ec7n t\u00edch v\u00e0 khu v\u1ef1c thi\u1ebft k\u1ebf. Th\u00f4ng th\u01b0\u1eddng khu v\u1ef1c ph\u00f2ng th\u00ed nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed s\u1ebd c\u00f3 di\u1ec7n t\u00edch t\u1ed1i thi\u1ec3u 20m2<\/sup> tr\u1edf l\u00ean.<\/p>\n\n\n\n

                \"\"
                Figure 2: <\/strong>S\u01a1 \u0111\u1ed3 m\u1eabu c\u00e1c ph\u00f2ng \u0111\u01b0\u1ee3c ph\u00e2n chia trong ph\u00f2ng th\u00ed nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed<\/em><\/figcaption><\/figure>\n\n\n\n

                4. D\u1ecbch v\u1ee5 c\u1ee7a ABT t\u01b0 v\u1ea5n thi\u1ebft k\u1ebf ph\u00f2ng th\u00ed nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed \u0111\u1ea1t chu\u1ea9n<\/strong><\/h2>\n\n\n\n

                ABT cung c\u1ea5p d\u1ecbch v\u1ee5 t\u01b0 v\u1ea5n thi\u1ebft k\u1ebf v\u00e0 x\u00e2y d\u1ef1ng ph\u00f2ng th\u00ed nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed \u0111\u1ea1t chu\u1ea9n theo t\u1eebng nhu c\u1ea7u v\u00e0 c\u1ea5u tr\u00fac khu v\u1ef1c c\u1ee7a kh\u00e1ch h\u00e0ng. V\u1edbi \u0111\u1ed9i ng\u0169 k\u1ef9 thu\u1eadt gi\u00e0u kinh nghi\u1ec7m, ch\u00fang t\u00f4i lu\u00f4n s\u1eb5n s\u00e0ng \u0111\u1ebfn n\u01a1i kh\u1ea3o s\u00e1t, h\u1ed7 tr\u1ee3 t\u01b0 v\u1ea5n mi\u1ec5n ph\u00ed c\u0169ng nh\u01b0 thi\u1ebft k\u1ebf s\u01a1 \u0111\u1ed3 c\u00e1c ph\u00f2ng, v\u1ecb tr\u00ed trang thi\u1ebft b\u1ecb ph\u00f9 h\u1ee3p v\u00e0 t\u1ed1i \u01b0u nh\u1ea5t.<\/p>\n\n\n\n

                For the fastest support, please contact hotline 0903.307.258<\/p>","protected":false},"excerpt":{"rendered":"

                Kh\u00e1m ph\u00e1 c\u00e1c ti\u00eau chu\u1ea9n c\u1ea7n thi\u1ebft \u0111\u1ec3 duy tr\u00ec ph\u00f2ng th\u00ed nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed an to\u00e0n v\u00e0 hi\u1ec7u qu\u1ea3. H\u01b0\u1edbng d\u1eabn chi ti\u1ebft v\u1ec1 an to\u00e0n, thi\u1ebft k\u1ebf, quy tr\u00ecnh v\u00e0 qu\u1ea3n l\u00fd ch\u1ea5t l\u01b0\u1ee3ng.<\/p>","protected":false},"author":1,"featured_media":11243,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Team","author_link":"https:\/\/abtvn.com\/en\/author\/admin\/"},"uagb_comment_info":0,"uagb_excerpt":"Kh\u00e1m ph\u00e1 c\u00e1c ti\u00eau chu\u1ea9n c\u1ea7n thi\u1ebft \u0111\u1ec3 duy tr\u00ec ph\u00f2ng th\u00ed nghi\u1ec7m sinh h\u1ecdc ph\u00e2n t\u1eed an to\u00e0n v\u00e0 hi\u1ec7u qu\u1ea3. H\u01b0\u1edbng d\u1eabn chi ti\u1ebft v\u1ec1 an to\u00e0n, thi\u1ebft k\u1ebf, quy tr\u00ecnh v\u00e0 qu\u1ea3n l\u00fd ch\u1ea5t l\u01b0\u1ee3ng.","_links":{"self":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/10314","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/comments?post=10314"}],"version-history":[{"count":15,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/10314\/revisions"}],"predecessor-version":[{"id":13098,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/posts\/10314\/revisions\/13098"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media\/11243"}],"wp:attachment":[{"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/media?parent=10314"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/categories?post=10314"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/abtvn.com\/en\/wp-json\/wp\/v2\/tags?post=10314"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}