Introduction:
Hepatis B Virus is a member of the Hepadnaviridae family of viruses with a double-stranded DNA structure. It is estimated that 350 million people are chronically infected with HBV each year. Patients with chronic hepatitis can develop cirrhosis and liver cancer. Therefore, accurate detection and quantification of HBV in blood will make treatment easier for the patients.
TopSENSI ® HBV qPCR Kit is a test kit based on real-time PCR method that allows accurate detection and quantification of HBV concentration in human serum/plasma. The standard curve is built based on 5 points which helps provide the most accurate quantitative results. The kit has the
HIGHLIGHTS
- Simple process easy manipulation
- Process The time to perform PCR is fast, only about 1 hour and 30 minutes, and it's easy to do
- High specificity, sensitivity (Detectability up to 25 IU/mL)
- Taqman probe technology
SPECIFICATIONS
| Target | Hepatitis B Virus (HBV) |
| Input sample | DNA after extraction from initial serum and plasma samples |
| Input sample volume | 10µL DNA |
| Color channel detection | FAM: detect HBV
HEX: detect internal control |
| Sensitivity | 25 IU/ ml |
| Linear range | 10⁹ – 2.5×10 copies/mL |
| Technology | TaqMan probe |
| Standard curve | (E1: 10¹) (E2: 10²) (E3: 10³) (E4: 10⁴)(E5: 10⁵) |
| PCR time | 1h30m |
| Specificity | only detect HBV |
| Ingredient | HBV qPCR mix, HBV standard ( E1, E2,E3,E4,E5), Negative control, Internal control, Tube PCR. |
| Storage | 12 months with -20°CoC |
DATA EXPERIMENT
Figure 1Real-time PCR results to assess the sensitivity of the HBV test kit (ABT)
Fig 2Real-time PCR results evaluate the repeatability of the HBV test kit (ABT)
Figure 3Real-time PCR results of patients infected with Hepatitis B virus.
Figure 4: : Real-time PCR analysis on HBV samples using LightCycler® 96 system at a standard laboratory.Results of quality assessment between the ABT kit and the Rocher supplier's kit on a sample of 86 patients who were positive for HBV. The results show that the TopSENSI ® HBV qPCR kit has the same quality as Roche (Assessment on the same reference system).
