TopSENSI® HBV qPCR KIT (SQH-101)

Purpose: using for quantification of Hepatitis B virus (HBV) DNA in human serum samples by Real-time PCR

Storage conditions: -20°CoC

Packing: chemicals are not ready or not divided

Manufacturer: ABT (Vietnam)

Code: SQH-101 Categories ,

Introduction:

Hepatis B Virus is a member of the Hepadnaviridae family of viruses with a double-stranded DNA structure. It is estimated that 350 million people are chronically infected with HBV each year. Patients with chronic hepatitis can develop cirrhosis and liver cancer. Therefore, accurate detection and quantification of HBV in blood will make treatment easier for the patients.

TopSENSI ® HBV qPCR Kit is a test kit based on real-time PCR method that allows accurate detection and quantification of HBV concentration in human serum/plasma. The standard curve is built based on 5 points which helps provide the most accurate quantitative results. The kit has the

 

HIGHLIGHTS

  • Simple process easy manipulation
  • Process The time to perform PCR is fast, only about 1 hour and 30 minutes, and it's easy to do
  • High specificity, sensitivity (Detectability up to 25 IU/mL)
  • Taqman probe technology

 

SPECIFICATIONS

Target Hepatitis B Virus (HBV)
Input sample DNA after extraction from initial serum and plasma samples
Input sample volume 10µL DNA
Color channel detection FAM: detect HBV

HEX: detect internal control

Sensitivity 25 IU/ ml
Linear range 10⁹ – 2.5×10 copies/mL
Technology TaqMan probe
Standard curve (E1: 10¹) (E2: 10²) (E3: 10³) (E4: 10⁴)(E5: 10⁵)
PCR time 1h30m
Specificity only detect HBV
Ingredient HBV qPCR mix, HBV standard ( E1, E2,E3,E4,E5), Negative control, Internal control, Tube PCR.
Storage 12 months with -20°CoC

 

 

DATA EXPERIMENT

kit HBV

Figure 1Real-time PCR results to assess the sensitivity of the HBV test kit (ABT)

kit HBV

Fig 2Real-time PCR results evaluate the repeatability of the HBV test kit (ABT)

kit-HBV

Figure 3Real-time PCR results of patients infected with Hepatitis B virus.

kit HBV

Figure 4: : Real-time PCR analysis on HBV samples using LightCycler® 96 system at a standard laboratory.Results of quality assessment between the ABT kit and the Rocher supplier's kit on a sample of 86 patients who were positive for HBV. The results show that the TopSENSI ® HBV qPCR kit has the same quality as Roche (Assessment on the same reference system).

SQH-101.C

50 tests, Chỉ master mix (không tách chiết). Mix dạng chia sẵn

SQH-101.D

50 tests, Chỉ master mix (không tách chiết). Mix dạng chưa chia

Manual

Click here: SQH-101

ABT BIOLOGICAL SOLUTIONS COMPANY LIMITED

ABT is a biotechnology company specializing in providing leading support solutions in the field of molecular bio diagnostics. At the same time, we are also a bridge to bring biological research achievements closer to reality, especially in preventing the Covid-19 pandemic.

Contact us

Workshop 5.02 – 6.07, Lot L2, Long Hau - Hiep Phuoc Street, Long Hau Industrial Park, Long Hau Ward, Can Giuoc Ward, Long An Province.

Số 9, Đường 17B, Phường Bình Trị Đông B, Quận Bình Tân, TP.HCM

0903 307 258​

sales@abt-vn.net
info@abt-vn.net​

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