Introduction to TopSPEC® WSSV – MBV qPCR Kit
White spot disease caused by the virus responsible for White Spot Syndrome (White Spot Syndrome Virus – WSSV). This is a highly virulent, double-stranded DNA (dsDNA) virus, capable of causing up to 100% mortality in shrimp populations within 3 to 10 days post-onset of clinical symptoms. Due to its rapid horizontal and vertical transmission rates, WSSV poses a primary threat to aquaculture ponds.
Shrimp stunting disease is caused by MBV (Monodon Baculovirus), a circular double-stranded DNA virus. They specifically parasitize and damage the epithelial structure of the hepatopancreatic tubules. Although the direct mortality rate is low, MBV induces immunosuppression, leading to stunted growth, severe size disparity, and increased susceptibility to secondary bacterial infections.
TopSPEC® WSSV – MBV qPCR Kit facilitates the detection of WSSV (VP466 gene) and MBV (Hypothetical protein gene) in shrimp samples using qPCR technology. This kit serves as an optimized diagnostic tool for post-larvae quality control and early pathogen detection during the pre-clinical stage.
TopSPEC® WSSV – MBV qPCR Kit
Highlights
- Simple procedure
- Optimize the PCR time to 1.5 hours.
- Various input sample types
- High specificity and sensitivity
Specification
| Targets |
|
| Input sample | DNA after extraction |
| Thể tích DNA đầu vào | 5 µL |
| Color channel detection | FAM: MBV detection Cy5: WSSV detection HEX: internal control (IC) |
| Technology | TaqMan probe |
| PCR time | 1.5h |
| Specificity | Multiplex kit for simultaneous detection of WSSV and MBV |
| Component | MW qPCR mix, Negative Control, MW Positive Control, Internal Control (IC), PCR tubes |
| Storage | 12 months at -20oC |
Recommended extraction kit: TopPURE® Highpure Viral Extraction Kit
Read more: Instructional Video for Qualitative Real-time PCR Kit
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