About TopSENSI ® Enterovirus71 RT-qPCR Kit
Bệnh tay chân miệng là bệnh truyền nhiễm do virus đường ruột thuộc Chi Enterovirus, họ Picornaviridae gây ra, trong đó thường gặp nhất là Coxsackie virus A6, A10, A16 và Enterovirus 71 (EV71). Các virus này không có vỏ bọc và có vật chất di truyền là RNA đơn dương (ssRNA+).
They typically propagate rapidly through the fecal-oral route or via direct contact with respiratory secretions, saliva, or the feces of infected individuals. Upon infection, these viruses cause mucocutaneous lesions in areas such as the oral cavity, palms, soles, buttocks, and knees. Notably, EV71 is frequently associated with severe complications, including neurological pathologies and fatalities. While no specific therapeutic treatment currently exists, vaccines have been developed and implemented for disease prevention.
TopSENSI ® Enterovirus71 RT-qPCR Kit is a diagnostic assay designed for the detection of the Enterovirus 71 - VP1 gene, the causative agent of HFMD, utilizing the RT-qPCR technique. The kit offers several advantages, including a streamlined and rapid workflow, along with high sensitivity and specificity.
HIGHLIGHTS
- Simple process easy manipulation
- The time to perform PCR is fast, only about 1 hour and 30 minutes
- High specificity and sensitivity

TopSENSI® Enterovirus71 RT-qPCR Kit (SQH-020)
SPECIFICATIONS
| Targets | Enterovirus 71 (EV71) – VP1 gene |
| Extraction sample type |
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| PCR sample volume | 10 µL |
| Fluorescent channels | FAM: Enterovirus 71 (EV71) – VP1 gene HEX: Internal control |
| Technology | TaqMan probe |
| PCR time | 1 hour 45 minutes |
| Sensitivity | 30,00 copies/reaction |
| Analytical Specificity | The kit is specifically designed for the detection of pathogens associated with hand, foot, and mouth disease. |
| Storage | 12 months at -20oC |
| Kit components | EV71 RT-qPCR Master Mix. Negative Control, Positive Control, Internal Control (IC), Tube PCR |
Recommended extraction kit
| Silica column extraction | TopPURE® Stool DNA Extraction Kit |
| TopPURE® Viral DNA/RNA Extraction Kit | |
| Automatic magbead extraction | TopPURE® Maga Viral DNA/RNA Extraction Kit |
Read more: Instructional Video for Qualitative Real-time PCR Kit




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